An innovative standard for LC-MS-based HCP-profiling and accurate quantity assessment: Application to batch consistency in viral vaccine samples.

abstract：:We present here the first proteomics analysis of uveal melanoma (UM) cells. These cells represent a good model for the identification of polypeptide markers, which could be developed as diagnostic tools. UM is the most common primary intraocular tumour in adults. In contrast to other cancers, the survival rate of pati...

journal_title：Proteomics

authors： Pardo M,García A,Thomas B,Piñeiro A,Akoulitchev A,Dwek RA,Zitzmann N

更新日期：2005-12-01 00:00:00

abstract：:We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...

journal_title：Proteomics

authors： Cramer R,Corless S

更新日期：2005-02-01 00:00:00

abstract：:Isolated from Elephantopus scaber L., a Chinese medicinal herb that is widely used to prevent and treat cancers in China, isodeoxyelephantopin (ESI) exerted antitumor effects on several cancer cells. However, its antitumor mechanism is still not clear. In this study, we found that ESI could induce G2/M arrest and subs...

journal_title：Proteomics

authors： Yan GR,Tan Z,Wang Y,Xu ML,Yu G,Li Y,He QY

更新日期：2013-11-01 00:00:00

abstract：:Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and sub-tropical oceans. It is unique in that it exclusively fixes N(2) at daytime, although it belongs to the non-heterocystous filamentous segment of the...

journal_title：Proteomics

authors： Sandh G,Ran L,Xu L,Sundqvist G,Bulone V,Bergman B

更新日期：2011-02-01 00:00:00

abstract：:The Annual Spring workshop of the HUPO-PSI was this year held at the EMBL International Centre for Advanced Training (EICAT) in Heidelberg, Germany. Delegates briefly reviewed the successes of the group to date. These include the wide spread implementation of the molecular interaction data exchange formats, PSI-MI XML...

journal_title：Proteomics

authors： Orchard S,Albar JP,Deutsch EW,Eisenacher M,Vizcaíno JA,Hermjakob H

更新日期：2011-11-01 00:00:00

abstract：:A novel strain of influenza A H1N1 emerged in the spring of 2009 and has spread rapidly throughout the world. Although vaccines have recently been developed that are expected to be protective, their availability was delayed until well into the influenza season. Although anti-influenza drugs such as neuraminidase inhib...

journal_title：Proteomics

authors： Chen CH,Zhang XQ,Lo CW,Liu PF,Liu YT,Gallo RL,Hsieh MF,Schooley RT,Huang CM

更新日期：2010-06-01 00:00:00

abstract：:Heat shock proteins (HSP) are families of highly conserved proteins which are induced in cells and tissues upon exposure to extreme conditions causing acute or chronic stress. They exhibit distinct functions and have been implicated in the pathogenesis of a number of diseases, including cancer. A causal relationship b...

journal_title：Proteomics

authors： Lichtenfels R,Kellner R,Bukur J,Beck J,Brenner W,Ackermann A,Seliger B

更新日期：2002-05-01 00:00:00

abstract：:Mass spectrometry-based proteomics is used to gain insight into the abundance and subcellular localization of cellular signaling components, the composition of molecular complexes and the regulation of signaling pathways. Multicellular organisms have evolved signaling networks and fast responses to stimuli that can be...

journal_title：Proteomics

authors： Kaufmann K,Smaczniak C,de Vries S,Angenent GC,Karlova R

更新日期：2011-02-01 00:00:00

abstract：:Many proteomic studies focus on quantitative aspects, using different stable isotope labeling techniques that require specialized software to analyze the generated data. Here we present jTraqX, an easy-to-use tool for processing and visualizing protein quantification data. jTraqX is platform independent and is compati...

journal_title：Proteomics

authors： Muth T,Keller D,Puetz SM,Martens L,Sickmann A,Boehm AM

更新日期：2010-03-01 00:00:00

abstract：:Histidine phosphorylation is a reversible post-translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N-linked phosphorylated amino acids. In an effort to help elucidate the heretofore hi...

journal_title：Proteomics

authors： Gao Y,Lee H,Kwon OK,Cheng Z,Tan M,Kim KT,Lee S

更新日期：2019-05-01 00:00:00

abstract：:In the era of quantitative proteomics, where mass spectrometry plays a pivotal role, in particular associated with the use of data-independent acquisition, it is time to perform an overview of this growing field with special focus on one of the most promising approaches: SWATH-MS, and to present future perspectives fo...

journal_title：Proteomics

authors： Anjo SI,Santa C,Manadas B

更新日期：2017-02-01 00:00:00

abstract：:Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...

journal_title：Proteomics

authors： Hare NJ,Chan B,Chan E,Kaufman KL,Britton WJ,Saunders BM

更新日期：2015-09-01 00:00:00

abstract：:In this study, a method, based on dual column affinity chromatography hyphenated to isotope dilution inductively coupled plasma-quadrupole MS, was developed for selenium determination in selenoprotein P, glutathione peroxidase, and selenoalbumin in human serum samples from a group of healthy volunteers (n=399). Method...

journal_title：Proteomics

authors： Letsiou S,Lu Y,Nomikos T,Antonopoulou S,Panagiotakos D,Pitsavos C,Stefanadis C,Pergantis SA

更新日期：2010-10-01 00:00:00

abstract：:The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...

journal_title：Proteomics

authors： Ortsäter H,Sundsten T,Lin JM,Bergsten P

更新日期：2007-09-01 00:00:00

abstract：:Total parenteral nutrition (TPN) is provided as the primary nitrogen source to manage patients with intestinal failure who were not able to sustain themselves on enteral feeds. The most common complication of long-term TPN use is hepatitis. A proteomic approach was used to identify proteins that are differentially exp...

journal_title：Proteomics

authors： Tsai JJ,Kuo HC,Lee KF,Tsai TH

更新日期：2015-11-01 00:00:00

abstract：:Recent advances in two-dimensional electrophoresis (2-DE) such as fluorescent 2-D differential gel electrophoresis (2-D DIGE) has made it possible to detect and quantitate the critical changes involved in disease pathogenesis. We have previously identified novel proteins with altered expression in primary colorectal c...

journal_title：Proteomics

authors： Nishimori T,Tomonaga T,Matsushita K,Oh-Ishi M,Kodera Y,Maeda T,Nomura F,Matsubara H,Shimada H,Ochiai T

更新日期：2006-02-01 00:00:00

abstract：:The present review deals with the use of combinatorial ligand libraries, composed by hexapeptides, in the capture and concentration of the low-abundance proteome. This method, first reported in 2005, is compared with other current methodologies aimed at exploring the "deep proteome", such as: depletion of high-abundan...

journal_title：Proteomics

authors： Boschetti E,Righetti PG

更新日期：2009-03-01 00:00:00

abstract：:Treatment of patients with malignant melanoma with interferon-alpha achieves a response in a small but significant subset of patients. Currently, although much is known about interferon biology, little is known about either the particular mechanisms of interferon-alpha activity that are crucial for response or why onl...

journal_title：Proteomics

authors： Craven RA,Stanley AJ,Hanrahan S,Totty N,Jackson DP,Popescu R,Taylor A,Frey J,Selby PJ,Patel PM,Banks RE

更新日期：2004-12-01 00:00:00

abstract：:Toxic oil syndrome (TOS) is a disease that appeared in Spain in 1981. It affected more than 20 000 people and produced over 300 deaths in the first 2 years. In this paper, a prospective study on the differences in gene expression in sera between a control versus a TOS-affected population, both originally exposed to th...

journal_title：Proteomics

authors： Quero C,Colomé N,Prieto MR,Carrascal M,Posada M,Gelpí E,Abian J

更新日期：2004-02-01 00:00:00

abstract：:The sample preparation procedures established for Prorocentrum triestinum were adapted to cover both thecate and athecate dinoflagellates. Further, whether trichloroacetic acid (TCA) precipitation can be used to fix and preserve the harmful or nuisance species from local waters that they infest was tested. Optimized t...

journal_title：Proteomics

authors： Chan LL,Hodgkiss IJ,Lu S,Lo SC

更新日期：2004-01-01 00:00:00

abstract：:The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better unders...

journal_title：Proteomics

authors： Victor B,Kanobana K,Gabriël S,Polman K,Deckers N,Dorny P,Deelder AM,Palmblad M

更新日期：2012-06-01 00:00:00

abstract：:The soluble fraction of nuclear proteins is a functionally significant fraction, since it has been shown that it contains ribonucleoproteins active in nuclear RNA metabolism. The aim of this work was to detect variations associated with cell proliferation, by comparing two-dimensional proteomes obtained from the solub...

journal_title：Proteomics

authors： González-Camacho F,Medina FJ

更新日期：2004-02-01 00:00:00

abstract：:Two new iodoacetamide-substituted cyanines, C3NIASO3 and C5NIASO3, were synthesized starting from hemicyanine and were utilized for labeling plasma proteins. Specificity, sensitivity and feasibility for SH residues was tested utilizing an equimolar mixture of standard proteins and with normal plasma. Oxidized plasma p...

journal_title：Proteomics

authors： Bruschi M,Grilli S,Candiano G,Fabbroni S,Della Ciana L,Petretto A,Santucci L,Urbani A,Gusmano R,Scolari F,Ghiggeri GM

更新日期：2009-01-01 00:00:00

abstract：:Aluminum (Al) toxicity is a major constraint for plant root development and growth as well as crop yield in acidic soils, which constitute approximately 40% of the potentially arable lands worldwide. The mechanisms of Al tolerance in plants are not well understood. As a whole systems approach, proteomic techniques hav...

journal_title：Proteomics

authors： Zheng L,Lan P,Shen RF,Li WF

更新日期：2014-03-01 00:00:00

abstract：:Experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. Channel catfish herpesvirus (CCV) is a simple comparative model for understanding herp...

journal_title：Proteomics

authors： Kunec D,Nanduri B,Burgess SC

更新日期：2009-05-01 00:00:00

abstract：:Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to allev...

journal_title：Proteomics

authors： Colignon B,Raes M,Dieu M,Delaive E,Mauro S

更新日期：2013-07-01 00:00:00

abstract：:Automated multidimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly applied in various large scale proteome profiling efforts. However, comprehensive global proteome analysis remains technically challenging due to issues associated with sample complexity and dynamic ran...

journal_title：Proteomics

authors： Liu T,Qian WJ,Chen WN,Jacobs JM,Moore RJ,Anderson DJ,Gritsenko MA,Monroe ME,Thrall BD,Camp DG 2nd,Smith RD

更新日期：2005-04-01 00:00:00

abstract：:The quality of samples and of pre-analytical steps are crucial in all biological tests, this is dramatically true in proteomics analysis. In renal cell carcinoma preparation for two-dimensional gel electrophoresis the time elapsed between sample collection and treatment, and the heterogeneity of tissues are considered...

journal_title：Proteomics

authors： Sarto C,Valsecchi C,Mocarelli P

更新日期：2002-11-01 00:00:00

abstract：:Phosphorylation of proteins is one of the most prominent PTMs and for instance a key regulator of signal transduction. In order to improve our understanding of cellular phosphorylation events, considerable effort has been devoted to improving the analysis of phosphorylation by MS-based proteomics. Different enrichment...

journal_title：Proteomics

authors： Eyrich B,Sickmann A,Zahedi RP

更新日期：2011-02-01 00:00:00

abstract：:The hepatitis B virus X-protein (HBx), a multifunctional viral regulator, participates in the viral life cycle and in the development of hepatocellular carcinoma (HCC). We previously reported a high incidence of HCC in transgenic mice expressing HBx. In this study, proteomic analysis was performed to identify proteins...

journal_title：Proteomics

authors： Kim SY,Lee PY,Shin HJ,Kim DH,Kang S,Moon HB,Kang SW,Kim JM,Park SG,Park BC,Yu DY,Bae KH,Lee SC

更新日期：2009-11-01 00:00:00