Identification of proteins regulated by interferon-alpha in resistant and sensitive malignant melanoma cell lines.

abstract：:The sample preparation procedures established for Prorocentrum triestinum were adapted to cover both thecate and athecate dinoflagellates. Further, whether trichloroacetic acid (TCA) precipitation can be used to fix and preserve the harmful or nuisance species from local waters that they infest was tested. Optimized t...

journal_title：Proteomics

authors： Chan LL,Hodgkiss IJ,Lu S,Lo SC

更新日期：2004-01-01 00:00:00

abstract：:To implement the 2-DE database of serogroup A Neisseria meningitidis (MenA) and improve its potential of investigation in bacterial biology, cell extracts were separated by tricine-SDS-PAGE and 131 novel proteins were identified by microLC-ESI-IT-MS/MS. These identifications extended to 404, the number of MenA gene ex...

journal_title：Proteomics

authors： Bernardini G,Arena S,Braconi D,Scaloni A,Santucci A

更新日期：2007-09-01 00:00:00

abstract：:Post-translational methylation of the epsilon-amino group of lysine residues regulates a number of protein functions. Calmodulin, a key modulator of intracellular calcium signaling, is methylated on lysine 115 in many species. Although the amino acid sequence of calmodulin is highly conserved in eukaryotes, it has bee...

journal_title：Proteomics

authors： Takemori N,Komori N,Thompson JN Jr,Yamamoto MT,Matsumoto H

更新日期：2007-08-01 00:00:00

abstract：:Diurnal physiological acclimation regulated by a circadian system is an advantage for plant fitness. The circadian system is composed of a signal input, the clock and output pathways. Understanding the regulation mechanism of the output pathways remains a major challenge. Diurnal proteomic change reflects the state of...

journal_title：Proteomics

authors： Wang Z,Wang T

更新日期：2011-01-01 00:00:00

abstract：:Familial hemiplegic migraine type 1 (FHM1) is caused by missense mutations in the CACNA1A gene that encodes the alpha1A pore-forming subunit of Ca(V)2.1 Ca(2+) channels. Knock-in (KI) transgenic mice expressing Ca(V)2.1 Ca(2+) channels with a human pathogenic FHM1 mutation reveal enhanced glutamatergic neurotransmissi...

journal_title：Proteomics

authors： Klychnikov OI,Li KW,Sidorov IA,Loos M,Spijker S,Broos LA,Frants RR,Ferrari MD,Mayboroda OA,Deelder AM,Smit AB,van den Maagdenberg AM

更新日期：2010-07-01 00:00:00

abstract：:Scanning MS by MALDI MS imaging (MALDI-MSI) creates large volumetric global datasets that describe the location and identity of ions registered at each sampling location. While thousands of ion peaks are recorded in a typical whole-tissue analysis, only a fraction of these measured molecules are purposefully scrutiniz...

journal_title：Proteomics

authors： Fehniger TE,Suits F,Végvári Á,Horvatovich P,Foster M,Marko-Varga G

更新日期：2014-04-01 00:00:00

abstract：:Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt-tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV-irradiated Agropyron elongatum (...

journal_title：Proteomics

authors： Wang MC,Peng ZY,Li CL,Li F,Liu C,Xia GM

更新日期：2008-04-01 00:00:00

abstract：:As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restr...

journal_title：Proteomics

authors： Ge P,Hao P,Cao M,Guo G,Lv D,Subburaj S,Li X,Yan X,Xiao J,Ma W,Yan Y

更新日期：2013-10-01 00:00:00

abstract：:Two new iodoacetamide-substituted cyanines, C3NIASO3 and C5NIASO3, were synthesized starting from hemicyanine and were utilized for labeling plasma proteins. Specificity, sensitivity and feasibility for SH residues was tested utilizing an equimolar mixture of standard proteins and with normal plasma. Oxidized plasma p...

journal_title：Proteomics

authors： Bruschi M,Grilli S,Candiano G,Fabbroni S,Della Ciana L,Petretto A,Santucci L,Urbani A,Gusmano R,Scolari F,Ghiggeri GM

更新日期：2009-01-01 00:00:00

abstract：:Identification and analysis of types of biological protein-protein interactions and their interfaces to predict obligate and non-obligate complexes is a problem that has drawn the attention of the research community in the past few years. In this paper, we propose a prediction approach to predict these two types of co...

journal_title：Proteomics

authors： Aziz MM,Maleki M,Rueda L,Raza M,Banerjee S

更新日期：2011-10-01 00:00:00

abstract：:A characteristic of two-dimensional proteomics gels is a general bimodal distribution of isoelectric (pI) values. Discussion of this feature has focussed on the balance of acidic and basic ionisable residues, and potential relationships between pI distributions and organism classification or protein subcellular locati...

journal_title：Proteomics

authors： Chan P,Lovrić J,Warwicker J

更新日期：2006-06-01 00:00:00

abstract：:To date, proteomics approaches have aimed to either identify novel proteins or change in protein expression/modification in various organisms under normal or disease conditions. One major aspect of functional proteomics is to identify protein biological properties in a given context, however, forward proteomics approa...

journal_title：Proteomics

authors： Palcy S,Chevet E

更新日期：2006-10-01 00:00:00

abstract：:Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% of CW mass) and proteins (5-10%) that play major roles in CW plasticity during development and in response to environmental cues. Here, we present CW proteomics data of Arabidopsis thaliana roots. Plants were cultivated in hydroponic conditio...

journal_title：Proteomics

authors： Nguyen-Kim H,San Clemente H,Balliau T,Zivy M,Dunand C,Albenne C,Jamet E

更新日期：2016-02-01 00:00:00

abstract：:Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate...

journal_title：Proteomics

authors： Molle V,Leiba J,Zanella-Cléon I,Becchi M,Kremer L

更新日期：2010-11-01 00:00:00

abstract：:Plant tissues contain large amounts of secondary compounds that significantly interfere with protein extraction and 2DE analysis. Thus, sample preparation is a crucial step prior to 2DE in plant proteomics. This tutorial highlights the guidelines that need to be followed to perform an adequate total protein extraction...

journal_title：Proteomics

authors： Wu X,Gong F,Wang W

更新日期：2014-03-01 00:00:00

abstract：:Egr1 (Zif268) is an immediate early gene encoding an inducible transcription factor involved in synaptic plasticity and several forms of memory in rodents. Using 2-DE and MS, we compared proteomes of hippocampal subregions and cortex in Egr1-deficient and wild-type littermates. Two significant differences were identif...

journal_title：Proteomics

authors： Chardonnet S,Decottignies P,Amar L,Le Caer JP,Davis S,Laroche S,Le Maréchal P

更新日期：2007-01-01 00:00:00

abstract：:With recent progress in the analysis of the salivary proteome, the number of salivary proteins identified has increased dramatically. However, the physiological functions of many of the newly discovered proteins remain unclear. Closely related to the study of a protein's function is the identification of its interacti...

journal_title：Proteomics

authors： Siqueira WL,Lee YH,Xiao Y,Held K,Wong W

更新日期：2012-11-01 00:00:00

abstract：:In prokaryotes, acyl carrier protein (ACP) is a cofactor central to a myriad of syntheses, including fatty acid and phospholipid synthesis. To fulfill its function, ACP must therefore interact with a multitude of different enzymes, which includes the thioesterase YbgC. We found a specific interaction between ACP and Y...

journal_title：Proteomics

authors： Gully D,Bouveret E

更新日期：2006-01-01 00:00:00

abstract：:Collision-activated dissociation and electron-transfer dissociation (ETD) each produce spectra containing unique features. Though several database search algorithms (e.g. SEQUEST, MASCOT, and Open Mass Spectrometry Search Algorithm) have been modified to search ETD data, this consists chiefly of the ability to search ...

journal_title：Proteomics

authors： Good DM,Wenger CD,Coon JJ

更新日期：2010-01-01 00:00:00

abstract：:Membrane proteins are key molecules in the cell, and are important targets for pharmaceutical drugs. Few three-dimensional structures of membrane proteins have been obtained, which makes computational prediction of membrane proteins crucial for studies of these key molecules. Here, seven membrane protein topology pred...

journal_title：Proteomics

authors： Fagerberg L,Jonasson K,von Heijne G,Uhlén M,Berglund L

更新日期：2010-03-01 00:00:00

abstract：:Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...

journal_title：Proteomics

authors： Ahsan N,Renaut J,Komatsu S

更新日期：2009-05-01 00:00:00

abstract：:Proteins determine the quality of barley in malting and brewing end-uses. In this regard, water-soluble barley proteins play a major role in the formation, stability, and texture of head foams. Our objective was to survey the barley seed proteins that could be involved in the foaming properties of beer. Therefore, two...

journal_title：Proteomics

authors： Perrocheau L,Rogniaux H,Boivin P,Marion D

更新日期：2005-07-01 00:00:00

abstract：:In this study, various proteomics-based methods were utilized to examine the 14-3-3 protein family in Arabidopsis thaliana. A protein extract was prepared from an Arabidopsis hypocotyl suspension culture and analyzed by two-dimensional gel electrophoresis and immunoblotting with a 14-3-3 monoclonal antibody that recog...

journal_title：Proteomics

authors： Fuller B,Stevens SM Jr,Sehnke PC,Ferl RJ

更新日期：2006-05-01 00:00:00

abstract：:Plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. Some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergisti...

journal_title：Proteomics

authors： da Silva AJ,Gómez-Mendoza DP,Junqueira M,Domont GB,Ximenes Ferreira Filho E,de Sousa MV,Ricart CA

更新日期：2012-08-01 00:00:00

abstract：:Automated multidimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly applied in various large scale proteome profiling efforts. However, comprehensive global proteome analysis remains technically challenging due to issues associated with sample complexity and dynamic ran...

journal_title：Proteomics

authors： Liu T,Qian WJ,Chen WN,Jacobs JM,Moore RJ,Anderson DJ,Gritsenko MA,Monroe ME,Thrall BD,Camp DG 2nd,Smith RD

更新日期：2005-04-01 00:00:00

abstract：:Oxidation of thiol proteins, which results in conversion of cysteine residues to cysteine sulfenic, sulfinic or sulfonic acids, is an important posttranslational control of protein function in cells. To facilitate the analysis of this process with MALDI-MS, we have developed a method for selective enrichment and ident...

journal_title：Proteomics

authors： Chang YC,Huang CN,Lin CH,Chang HC,Wu CC

更新日期：2010-08-01 00:00:00

abstract：:With advances in liquid chromatography coupled to tandem mass spectrometry technologies combined with the continued goals of biomarker discovery, clinical applications of established biomarkers, and integrating large multiomic datasets (i.e. "big data"), there remains an urgent need for robust tools to assess instrume...

journal_title：Proteomics

authors： Bereman MS

更新日期：2015-03-01 00:00:00

abstract：:Tyrosol (TYR, 2-(4-hydroxyphenyl)ethanol), one of the main phenols in olive oil and olive fruit, significantly strengthens resistance to thermal and oxidative stress in the nematode Caenorhabditis elegans and extends its lifespan. To elucidate the cellular functions regulated by TYR, we have used a proteomic procedure...

journal_title：Proteomics

authors： Cañuelo A,Peragón J

更新日期：2013-10-01 00:00:00

abstract：:Most information on molecular processes accompanying and driving adipocyte differentiation are derived from rodent models. Here, we provide a comprehensive analysis of combined transcriptomic and proteomic alterations during adipocyte differentiation in Simpson-Golabi-Behmel Syndrome (SGBS) cells. The SGBS cells are a...

journal_title：Proteomics

authors： Kalkhof S,Krieg L,Büttner P,Wabitsch M,Küntzel C,Friebe D,Landgraf K,Hanschkow M,Schubert K,Kiess W,Krohn K,Blüher M,von Bergen M,Körner A

更新日期：2020-05-08 00:00:00

abstract：:There is ample evidence for the involvement of protein phosphorylation on serine/threonine/tyrosine in bacterial signaling and regulation, but very few exact phosphorylation sites have been experimentally determined. Recently, gel-free high accuracy MS studies reported over 150 phosphorylation sites in two bacterial m...

journal_title：Proteomics

authors： Miller ML,Soufi B,Jers C,Blom N,Macek B,Mijakovic I

更新日期：2009-01-01 00:00:00