NetPhosBac - a predictor for Ser/Thr phosphorylation sites in bacterial proteins.

abstract：:Cardiac conduction defects were found in transgenic mice deficient in urea transporter UT-B. To investigate the molecular mechanisms of the conduction defects caused by UT-B deletion, we studied the protein expression profiles of heart tissue (comprising most conduction system) in wild-type versus UT-B null mice at di...

journal_title：Proteomics

authors： Yu H,Meng Y,Wang LS,Jin X,Gao LF,Zhou L,Ji K,Li Y,Zhao LJ,Chen GQ,Zhao XJ,Yang B

更新日期：2009-02-01 00:00:00

abstract：:High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to...

journal_title：Proteomics

authors： Majoul T,Bancel E,Triboï E,Ben Hamida J,Branlard G

更新日期：2003-02-01 00:00:00

abstract：:As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restr...

journal_title：Proteomics

authors： Ge P,Hao P,Cao M,Guo G,Lv D,Subburaj S,Li X,Yan X,Xiao J,Ma W,Yan Y

更新日期：2013-10-01 00:00:00

abstract：:A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...

journal_title：Proteomics

authors： Huelseweh B,Ehricht R,Marschall HJ

更新日期：2006-05-01 00:00:00

abstract：:The gut microbiome has been shown to play a significant role in human healthy and diseased states. The dynamic signaling that occurs between the host and microbiome is critical for the maintenance of host homeostasis. Analyzing the human microbiome with metaproteomics, metabolomics, and integrative multi-omics analyse...

journal_title：Proteomics

authors： Peters DL,Wang W,Zhang X,Ning Z,Mayne J,Figeys D

更新日期：2019-08-01 00:00:00

abstract：:The discovery of PTMs in proteins by MS requires nearly complete sequence coverage of the detected proteolytic peptides. Unfortunately, mass spectrometric analysis of the desired sequence fragments is often impeded due to low ionization efficiency and/or signal suppression in complex samples. When several lysine resid...

journal_title：Proteomics

authors： Markoutsa S,Bahr U,Papasotiriou DG,Häfner AK,Karas M,Sorg BL

更新日期：2014-03-01 00:00:00

abstract：:Prior to analysis by mass spectrometry, protein samples are often digested. Maximizing the peptide yield from digestion can increase the number of peptides detected and the confidence in protein identification. To determine the optimal conditions for digestion, the Michaelis-Menten kinetic parameters for Promega seque...

journal_title：Proteomics

authors： Finehout EJ,Cantor JR,Lee KH

更新日期：2005-06-01 00:00:00

abstract：:We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly to room temperature for short to medium periods of time. Sections from mouse brain were cut in a cryostat microtome, placed on a MALDI target and allowed to warm to room tempera...

journal_title：Proteomics

authors： Goodwin RJ,Dungworth JC,Cobb SR,Pitt AR

更新日期：2008-09-01 00:00:00

abstract：:In the post-genomic era, mass spectrometry is destined to fulfil a central role in biomedical research, and it is in the area of protein identification that mass spectrometry is now most rapidly expanding. An important identification method is to subject a protein to proteolysis and determine the resulting peptide mas...

journal_title：Proteomics

authors： Griffiths WJ,Jonsson AP

更新日期：2001-08-01 00:00:00

abstract：:In recent years, a new paradigm for genome annotation has emerged, termed "proteogenomics," that leverages peptide MS to annotate a genome. This is achieved by mapping peptides to a six-frame translation of a genome, including available splice databases, which may suggest refinements to gene models. Using this approac...

journal_title：Proteomics

authors： Chapman B,Bellgard M

更新日期：2014-12-01 00:00:00

abstract：:Rice is susceptible to cold stress and with a future of climatic instability we will be unable to produce enough rice to satisfy increasing demand. A thorough understanding of the molecular responses to thermal stress is imperative for engineering cultivars, which have greater resistance to low temperature stress. In ...

journal_title：Proteomics

authors： Neilson KA,Mariani M,Haynes PA

更新日期：2011-05-01 00:00:00

abstract：:The tuber of potato (Solanum tuberosum) is commonly used as a model for underground storage organs. In this study, changes in the proteome were followed from tuberization, through tuber development and storage into the sprouting phase. Data interrogation using principal component analysis was able to clearly discrimin...

journal_title：Proteomics

authors： Lehesranta SJ,Davies HV,Shepherd LV,Koistinen KM,Massat N,Nunan N,McNicol JW,Kärenlampi SO

更新日期：2006-11-01 00:00:00

abstract：:Total parenteral nutrition (TPN) is provided as the primary nitrogen source to manage patients with intestinal failure who were not able to sustain themselves on enteral feeds. The most common complication of long-term TPN use is hepatitis. A proteomic approach was used to identify proteins that are differentially exp...

journal_title：Proteomics

authors： Tsai JJ,Kuo HC,Lee KF,Tsai TH

更新日期：2015-11-01 00:00:00

abstract：:To exploit advances in proteomics for drug discovery, high-throughout methods for target validation that directly address the cellular roles of proteins are required. To do this, we have characterized fluorophore-assisted light inactivation (FALI) which uses coherent or diffuse light targeted by fluorescein-labeled pr...

journal_title：Proteomics

authors： Beck S,Sakurai T,Eustace BK,Beste G,Schier R,Rudert F,Jay DG

更新日期：2002-03-01 00:00:00

abstract：:Exosomes are deliverers of critically functional proteins, capable of transforming target cells in numerous cancers, including hepatocellular carcinoma (HCC). We hypothesize that the motility of HCC cells can be featured by comparative proteome of exosomes. Hence, we performed the super-SILAC-based MS analysis on the ...

journal_title：Proteomics

authors： Zhang J,Lu S,Zhou Y,Meng K,Chen Z,Cui Y,Shi Y,Wang T,He QY

更新日期：2017-07-01 00:00:00

abstract：:Protein-ligand interactions serve as fundamental regulators of numerous biological processes. Among protein-ligand pairs, glycan binding proteins (GBPs) and the glycans they recognize represent unique and highly complex interactions implicated in a broad range of regulatory activities. With few exceptions, cell surfac...

journal_title：Proteomics

authors： Kamili NA,Arthur CM,Gerner-Smidt C,Tafesse E,Blenda A,Dias-Baruffi M,Stowell SR

更新日期：2016-12-01 00:00:00

abstract：:We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HP...

journal_title：Proteomics

authors： Ro HS,Koh BH,Jung SO,Park HK,Shin YB,Kim MG,Chung BH

更新日期：2006-04-01 00:00:00

abstract：:In this work, we first immobilized tin(IV) ion on polydopamine-coated magnetic graphene (magG@PDA) to synthesize Sn4+ -immobilized magG@PDA (magG@PDA-Sn4+ ) and successfully applied the material to highly selective enrichment of phosphopeptides. The material gathered the advantages of large surface area of graphene, s...

journal_title：Proteomics

authors： Lin H,Deng C

更新日期：2016-11-01 00:00:00

abstract：:The authors present pathwayPCA, an R/Bioconductor package for integrative pathway analysis that utilizes modern statistical methodology, including supervised and adaptive, elastic-net, sparse principal component analysis. pathwayPCA can be applied to continuous, binary, and survival outcomes in studies with multiple c...

journal_title：Proteomics

authors： Odom GJ,Ban Y,Colaprico A,Liu L,Silva TC,Sun X,Pico AR,Zhang B,Wang L,Chen X

更新日期：2020-11-01 00:00:00

abstract：:Attribution of the most probable functions to proteins identified by proteomics is a significant challenge that requires extensive literature analysis. We have developed a system for automated prediction of implicit and explicit biologically meaningful functions for a proteomics study of the nucleolus. This approach u...

journal_title：Proteomics

authors： Schuemie M,Chichester C,Lisacek F,Coute Y,Roes PJ,Sanchez JC,Kors J,Mons B

更新日期：2007-03-01 00:00:00

abstract：:The nucleus is the organelle where basically all DNA-related processes take place in eukaryotes, such as replication, transcription, and splicing as well as epigenetic regulation. The identification and description of the nuclear proteins is one of the requisites toward a comprehensive understanding of the biological ...

journal_title：Proteomics

authors： Bigeard J,Rayapuram N,Bonhomme L,Hirt H,Pflieger D

更新日期：2014-10-01 00:00:00

abstract：:In theory, proteases with broad cleavage specificity could be applied to digest protein samples to improve the phosphoproteomic analysis coverage. However, in practice this approach is seldom employed. This is because the identification of phosphopeptides without enzyme specificity by conventional database search stra...

journal_title：Proteomics

authors： Dong M,Ye M,Cheng K,Dong J,Zhu J,Qin H,Bian Y,Zou H

更新日期：2014-11-01 00:00:00

abstract：:Sepsis is an extreme host response to infection that leads to loss of organ function and cardiovascular integrity. Mortality from sepsis is on the rise. Despite more than three decades of research and clinical trials, specific diagnostic and therapeutic strategies for sepsis are still absent. The use of LFQ- and TMT-b...

journal_title：Proteomics

authors： Pimienta G,Heithoff DM,Rosa-Campos A,Tran M,Esko JD,Mahan MJ,Marth JD,Smith JW

更新日期：2019-03-01 00:00:00

abstract：:Neurons present a highly polarized morphology, often displaying a significantly imbalanced distribution of the cytoplasm between the somatic and axonal domains. This imbalance requires cell-specific mechanisms for the maintenance of the axoplasmic mass during development, neuronal homeostasis, and recovery after injur...

journal_title：Proteomics

authors： Farias J,Sotelo JR,Sotelo-Silveira J

更新日期：2019-08-01 00:00:00

abstract：:Heat shock proteins (HSP) are families of highly conserved proteins which are induced in cells and tissues upon exposure to extreme conditions causing acute or chronic stress. They exhibit distinct functions and have been implicated in the pathogenesis of a number of diseases, including cancer. A causal relationship b...

journal_title：Proteomics

authors： Lichtenfels R,Kellner R,Bukur J,Beck J,Brenner W,Ackermann A,Seliger B

更新日期：2002-05-01 00:00:00

abstract：:Many tumors arising from epithelial tissues produce mucins, which readily come into contact with infiltrating cells in cancer tissues. MUC2 mucins were purified from the conditioned medium of a colorectal cancer cell line, LS180 cells. It is known that in cancer patients, the number of dendritic cells (DCs) is reduced...

journal_title：Proteomics

authors： Ishida A,Ohta M,Toda M,Murata T,Usui T,Akita K,Inoue M,Nakada H

更新日期：2008-08-01 00:00:00

abstract：:DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent ...

journal_title：Proteomics

authors： Timms JF,Cramer R

更新日期：2008-12-01 00:00:00

abstract：:Trichomonas vaginalis, a sexually transmitted parasite, has many cysteine proteinases (CPs); some are involved in trichomonal pathogenesis, express during infection, and antibodies against CPs have been detected in patient sera. The goal of this study was to identify the antigenic proteinases of T. vaginalis as potent...

journal_title：Proteomics

authors： Ramón-Luing LA,Rendón-Gandarilla FJ,Cárdenas-Guerra RE,Rodríguez-Cabrera NA,Ortega-López J,Avila-González L,Angel-Ortiz C,Herrera-Sánchez CN,Mendoza-García M,Arroyo R

更新日期：2010-02-01 00:00:00

abstract：:A curated set of 81 proven and 44 predicted lipoproteins of Escherichia coli K-12 was defined with the combined use of a literature survey, a variety of predictive tools and human expertise. The well-documented Gram-negative proteome of E. coli K-12 was chosen to assess how the different approaches complement each oth...

journal_title：Proteomics

authors： Gonnet P,Rudd KE,Lisacek F

更新日期：2004-06-01 00:00:00

abstract：:A simple and rapid method for characterizing hydrophobic integral membrane proteins and its utility for membrane proteomics using microcapillary liquid chromatography coupled on-line with tandem mass spectrometry (microLC-MS/MS) is described. The present technique does not rely on the use of detergents, strong organic...

journal_title：Proteomics

authors： Blonder J,Conrads TP,Yu LR,Terunuma A,Janini GM,Issaq HJ,Vogel JC,Veenstra TD

更新日期：2004-01-01 00:00:00