Abstract:
:The discovery of PTMs in proteins by MS requires nearly complete sequence coverage of the detected proteolytic peptides. Unfortunately, mass spectrometric analysis of the desired sequence fragments is often impeded due to low ionization efficiency and/or signal suppression in complex samples. When several lysine residues are in close proximity tryptic peptides may be too short for mass analysis. Moreover, modified peptides often appear in low stoichiometry and need to be enriched before analysis. We present here how the use of sulfo-NHS-SS-biotin derivatization of lysine side chain can help to detect PTMs in lysine-rich proteins. This label leads to a mass shift which can be adjusted by reduction of the SS bridge and alkylation with different reagents. Low intensity peptides can be enriched by use of streptavidin beads. Using this method, the functionally relevant protein kinase A phosphorylation site in 5-lipoxygenase was detected for the first time by MS. Additionally, methylation and acetylation could be unambiguously determined in histones.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Markoutsa S,Bahr U,Papasotiriou DG,Häfner AK,Karas M,Sorg BLdoi
10.1002/pmic.201300309subject
Has Abstractpub_date
2014-03-01 00:00:00pages
659-67issue
6eissn
1615-9853issn
1615-9861journal_volume
14pub_type
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