Immunoproteomics of the active degradome to identify biomarkers for Trichomonas vaginalis.

abstract：:Post-translational methylation of the epsilon-amino group of lysine residues regulates a number of protein functions. Calmodulin, a key modulator of intracellular calcium signaling, is methylated on lysine 115 in many species. Although the amino acid sequence of calmodulin is highly conserved in eukaryotes, it has bee...

journal_title：Proteomics

authors： Takemori N,Komori N,Thompson JN Jr,Yamamoto MT,Matsumoto H

更新日期：2007-08-01 00:00:00

abstract：:The determination of possible biomarkers in nasal secretion of healthy subjects can have a role in early diagnosis of diseases such as rhinosinusitis. For this purpose, nasal lavage fluids (NLFs) from ten volunteers, collected before and after they had been submitted to nasal provocations, were investigated. Separatio...

journal_title：Proteomics

authors： Casado B,Pannell LK,Iadarola P,Baraniuk JN

更新日期：2005-07-01 00:00:00

abstract：:Leishmaniasis is a zoonotic disease caused by the species of the genus Leishmania, flagellated protozoa that multiply inside mammalian macrophages and are transmitted by the bite of the sandfly. The disease is widespread and due to the lack of fully effective treatment and vaccination the search for new drugs and immu...

journal_title：Proteomics

authors： Dea-Ayuela MA,Rama-Iñiguez S,Bolás-Fernández F

更新日期：2006-07-01 00:00:00

abstract：:The recent development in immune checkpoint inhibitors and chimeric antigen receptor (CAR) T-cells in the treatment of cancer has not only demonstrated the potency of utilizing T-cell reactivity for cancer therapy, but has also highlighted the need for developing new approaches to discover targets suitable for such no...

journal_title：Proteomics

authors： Ternette N,Olde Nordkamp MJM,Müller J,Anderson AP,Nicastri A,Hill AVS,Kessler BM,Li D

更新日期：2018-06-01 00:00:00

abstract：:Large-scale interaction studies contribute the largest fraction of protein interactions information in databases. However, co-purification of non-specific or indirect ligands, often results in data sets that are affected by a considerable number of false positives. For the fraction of interactions mediated by short li...

journal_title：Proteomics

authors： Panni S,Montecchi-Palazzi L,Kiemer L,Cabibbo A,Paoluzi S,Santonico E,Landgraf C,Volkmer-Engert R,Bachi A,Castagnoli L,Cesareni G

更新日期：2011-01-01 00:00:00

abstract：:Plant epidermal trichomes are as varied in morphology as they are in function. In the halophyte Mesembryanthemum crystallinum, specialized trichomes called epidermal bladder cells (EBC) line the surface of leaves and stems, and increase dramatically in size and volume upon plant salt-treatment. These cells have been p...

journal_title：Proteomics

authors： Barkla BJ,Vera-Estrella R,Pantoja O

更新日期：2012-09-01 00:00:00

abstract：:Top-down proteomics have recently started to gain attention as a novel method to provide insight into the structure of proteins in their native state, specifically the number and location of disulfide bridges. However, previous techniques still relied on complex and time-consuming protein purification and reduction re...

journal_title：Proteomics

authors： Khairallah RJ,Sadayappan S

更新日期：2013-11-01 00:00:00

abstract：:We compared peptide identification by database (DB) search methods with de novo sequencing results for proteomics study in an organism without genome sequence information. When the former was done by searching the Expressed Sequence Tag (EST) DB of the sample organism or the NCBI nonredundant (nr) protein DB of green ...

journal_title：Proteomics

authors： Kwon KH,Kim M,Kim JY,Kim KW,Kim SI,Park YM,Yoo JS

更新日期：2003-12-01 00:00:00

abstract：:Selected or multiple reaction monitoring is a targeted mass spectrometry method (S/MRM-MS), in which many peptides are simultaneously and consistently analyzed during a single liquid chromatography-mass spectrometry (LC-S/MRM-MS) measurement. These capabilities make S/MRM-MS an attractive method to monitor a consisten...

journal_title：Proteomics

authors： Ebhardt HA,Sabidó E,Hüttenhain R,Collins B,Aebersold R

更新日期：2012-04-01 00:00:00

abstract：:FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...

journal_title：Proteomics

authors： Gattiker A,Bienvenut WV,Bairoch A,Gasteiger E

更新日期：2002-10-01 00:00:00

abstract：:We present a novel dataset assessing the specificity of protein-protein interactions between 69 transmitter and receiver domains from two-component system (TCS)-signalling pathways. TCS require a conserved protein-protein interaction between partner transmitter and receiver domains for signal transduction. The complex...

journal_title：Proteomics

authors： Whitworth DE,Millard A,Hodgson DA,Hawkins PF

更新日期：2008-05-01 00:00:00

abstract：:Glycan-binding proteins (GBPs) play an important role in cell adhesion, bacterial/viral infection, and cellular signaling pathways. However, little is known about the precision alteration of GBPs referred to pathological changes in hepatic stellate cells (HSCs) during liver fibrosis. Here, the carbohydrate microarrays...

journal_title：Proteomics

authors： Zhong Y,Qin Y,Dang L,Jia L,Zhang Z,Wu H,Cui J,Bian H,Li Z

更新日期：2015-10-01 00:00:00

abstract：:Scanning MS by MALDI MS imaging (MALDI-MSI) creates large volumetric global datasets that describe the location and identity of ions registered at each sampling location. While thousands of ion peaks are recorded in a typical whole-tissue analysis, only a fraction of these measured molecules are purposefully scrutiniz...

journal_title：Proteomics

authors： Fehniger TE,Suits F,Végvári Á,Horvatovich P,Foster M,Marko-Varga G

更新日期：2014-04-01 00:00:00

abstract：:Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...

journal_title：Proteomics

authors： Hare NJ,Chan B,Chan E,Kaufman KL,Britton WJ,Saunders BM

更新日期：2015-09-01 00:00:00

abstract：:Entamoeba histolytica is known for its extraordinary capacity to destroy human tissues, leading to invasive diseases such as ulcerative colitis or extra-intestinal abscesses. In order to identify the virulence factors of this parasite phenotypes and proteomes of two recently identified genetically related cell lines (...

journal_title：Proteomics

authors： Biller L,Schmidt H,Krause E,Gelhaus C,Matthiesen J,Handal G,Lotter H,Janssen O,Tannich E,Bruchhaus I

更新日期：2009-09-01 00:00:00

abstract：:Immune proteomics is an increasingly powerful tool for the investigation of the adaptive immune response to natural encounters between micro-organisms and their hosts. The versatile species Staphylococcus aureus serves to illustrate how these techniques can be employed to appreciate the complexity and diversity of the...

journal_title：Proteomics

authors： Bröker BM,van Belkum A

更新日期：2011-08-01 00:00:00

abstract：:Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient se...

journal_title：Proteomics

authors： Shi T,Su D,Liu T,Tang K,Camp DG 2nd,Qian WJ,Smith RD

更新日期：2012-04-01 00:00:00

abstract：:The lipid droplet (LD) is an organelle with vital functions found in nearly all organisms. LD proteomic research has provided fundamentally important insights into this organelle's functions. The review provides a summary of LD proteomic studies conducted across diverse organisms and cell and tissue types. The accumul...

journal_title：Proteomics

authors： Zhang C,Liu P

更新日期：2019-05-01 00:00:00

abstract：:MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...

journal_title：Proteomics

authors： Helsens K,Martens L,Vandekerckhove J,Gevaert K

更新日期：2007-02-01 00:00:00

abstract：:Mulberry dwarf (MD) is a serious infectious disease of mulberry caused by phytoplasma. Infection with MD phytoplasma results in stress phenotypes of yellowing, phyllody, stunting, proliferation, and witches' broom. Physiological and biochemical analysis has shown that infection with MD phytoplasma causes an increase i...

journal_title：Proteomics

authors： Ji X,Gai Y,Zheng C,Mu Z

更新日期：2009-12-01 00:00:00

abstract：:Geobacter sulfurreducens, generally considered to be a strict anaerobe, is a predominant microbe in subsurface environments, where it utilizes available metals as electron acceptors. To better understand the metabolic processes involved in the metal-reduction capability of this microbe, the proteins expressed by cells...

journal_title：Proteomics

authors： Khare T,Esteve-Núñez A,Nevin KP,Zhu W,Yates JR 3rd,Lovley D,Giometti CS

更新日期：2006-01-01 00:00:00

abstract：:Plasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two-thirds of the known protein targets for existing drugs. Consequently, defining membrane proteomes ...

journal_title：Proteomics

authors： Josic D,Clifton JG

更新日期：2007-08-01 00:00:00

abstract：:Phosphorylation of proteins is one of the most prominent PTMs and for instance a key regulator of signal transduction. In order to improve our understanding of cellular phosphorylation events, considerable effort has been devoted to improving the analysis of phosphorylation by MS-based proteomics. Different enrichment...

journal_title：Proteomics

authors： Eyrich B,Sickmann A,Zahedi RP

更新日期：2011-02-01 00:00:00

abstract：:As an initial screen for novel markers of renal cancer and to minimise background heterogeneity, we have compared the within-patient profiles of serum samples from seven patients pre- and post-nephrectomy. Samples were depleted of six of the most abundant proteins using Agilent's multiple affinity removal system (MARS...

journal_title：Proteomics

authors： Vasudev NS,Ferguson RE,Cairns DA,Stanley AJ,Selby PJ,Banks RE

更新日期：2008-12-01 00:00:00

abstract：:We have developed a proteome database (DB), BiomarkerDigger (http://biomarkerdigger.org) that automates data analysis, searching, and metadata-gathering function. The metadata-gathering function searches proteome DBs for protein-protein interaction, Gene Ontology, protein domain, Online Mendelian Inheritance in Man, a...

journal_title：Proteomics

authors： Jeong SK,Kwon MS,Lee EY,Lee HJ,Cho SY,Kim H,Yoo JS,Omenn GS,Aebersold R,Hanash S,Paik YK

更新日期：2009-07-01 00:00:00

abstract：:Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...

journal_title：Proteomics

authors： Ahsan N,Renaut J,Komatsu S

更新日期：2009-05-01 00:00:00

abstract：:Oxidation of thiol proteins, which results in conversion of cysteine residues to cysteine sulfenic, sulfinic or sulfonic acids, is an important posttranslational control of protein function in cells. To facilitate the analysis of this process with MALDI-MS, we have developed a method for selective enrichment and ident...

journal_title：Proteomics

authors： Chang YC,Huang CN,Lin CH,Chang HC,Wu CC

更新日期：2010-08-01 00:00:00

abstract：:The International Protein Index (IPI) database has been one of the most widely used protein databases in MS proteomics approaches. Recently, the closure of IPI in September 2011 was announced. Its recommended replacement is the new UniProt Knowledgebase (UniProtKB) "complete proteome" sets, launched in May 2011. Here,...

journal_title：Proteomics

authors： Griss J,Martín M,O'Donovan C,Apweiler R,Hermjakob H,Vizcaíno JA

更新日期：2011-11-01 00:00:00

abstract：:The hepatitis B virus X-protein (HBx), a multifunctional viral regulator, participates in the viral life cycle and in the development of hepatocellular carcinoma (HCC). We previously reported a high incidence of HCC in transgenic mice expressing HBx. In this study, proteomic analysis was performed to identify proteins...

journal_title：Proteomics

authors： Kim SY,Lee PY,Shin HJ,Kim DH,Kang S,Moon HB,Kang SW,Kim JM,Park SG,Park BC,Yu DY,Bae KH,Lee SC

更新日期：2009-11-01 00:00:00

abstract：:FtsH is the only membrane-bound and essential protease in Escherichia coli. It is responsible for the degradation of regulatory proteins and enzymes such as the heat-shock sigma factor RpoH or LpxC, the key enzyme of lipopolysaccharide biosynthesis. To find new FtsH targets, we trapped substrates in E. coli cells from...

journal_title：Proteomics

authors： Arends J,Thomanek N,Kuhlmann K,Marcus K,Narberhaus F

更新日期：2016-12-01 00:00:00