Abstract:
:In this study, a method, based on dual column affinity chromatography hyphenated to isotope dilution inductively coupled plasma-quadrupole MS, was developed for selenium determination in selenoprotein P, glutathione peroxidase, and selenoalbumin in human serum samples from a group of healthy volunteers (n=399). Method improvement was achieved using methanol-enhanced isotope dilution which resulted in improved sensitivity and removal of isobaric interferences. Although no human serum reference materials are currently certified for their selenium species levels, method development was conducted using human serum reference material BCR 637 and 639 as their Se species content has been reported in the previous studies, and thus comparisons were possible. The mean selenium concentrations determined for the 399 healthy volunteer serum samples were 23 ± 10 ng Se mL(-1) for glutathione peroxidase, 49 ± 15 ng Se mL(-1) for selenoprotein P and 11 ± 4 ng Se mL(-1) for selenoalbumin. These values are found to be in close agreement with published values for a limited number of healthy volunteer samples, and to establish baseline Se levels in serum proteins for an apparently healthy group of individuals, thus allowing for subsequent comparisons with respective values determined for groups of individuals with selenium related health issues, as well as assist in the discovery of potential selenium biomarkers. Also, the relationship between Se serum protein levels and some anthropometric characteristics of the volunteer population were investigated. Additionally, further development of the analytical method used in this study was achieved by adding a size exclusion chromatography column after the two affinity columns via a switching valve. This allowed for the separation of small selenium-containing molecules from glutathione peroxidase and thus enhanced the overall confidence in its identification.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Letsiou S,Lu Y,Nomikos T,Antonopoulou S,Panagiotakos D,Pitsavos C,Stefanadis C,Pergantis SAdoi
10.1002/pmic.200900677subject
Has Abstractpub_date
2010-10-01 00:00:00pages
3447-57issue
19eissn
1615-9853issn
1615-9861journal_volume
10pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::The effects of di(2-ethylhexyl) phthalate (DEHP) on proteins secreted by HepG2 cells were studied using a proteomic approach. HepG2 cells were exposed to various concentrations of DEHP (0, 2.5, 5, 10, 25, 50, 100, and 250 microM) for 24 or 48 h. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900674
更新日期:2010-05-01 00:00:00
abstract::Production of membrane proteins (MPs) is a challenging task as their hydrophobic nature and their specific requirements in cellular expression systems frequently prevent an efficient synthesis. Cell-free (CF) expression systems have been developed in recent times as promising tools by offering completely new approache...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800171
更新日期:2008-10-01 00:00:00
abstract::Cardiac conduction defects were found in transgenic mice deficient in urea transporter UT-B. To investigate the molecular mechanisms of the conduction defects caused by UT-B deletion, we studied the protein expression profiles of heart tissue (comprising most conduction system) in wild-type versus UT-B null mice at di...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701079
更新日期:2009-02-01 00:00:00
abstract::Protein phosphorylation on serine, threonine, and tyrosine is known to be involved in a wide variety of cellular processes, signal transduction, and bacterial virulence. We characterized, for the first time, the extracellular phosphoproteins of the Pseudomonas aeruginosa PA14 strain. We identified 28 phosphoproteins (...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400190
更新日期:2014-09-01 00:00:00
abstract::We compared peptide identification by database (DB) search methods with de novo sequencing results for proteomics study in an organism without genome sequence information. When the former was done by searching the Expressed Sequence Tag (EST) DB of the sample organism or the NCBI nonredundant (nr) protein DB of green ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300620
更新日期:2003-12-01 00:00:00
abstract::Advances in gel-based nonradioactive protein expression and PTM detection using fluorophores has served as the impetus for developing analytical instrumentation with improved imaging capabilities. We describe a CCD camera-based imaging instrument, equipped with both a high-pressure Xenon arc lamp and a UV transillumin...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500062
更新日期:2005-11-01 00:00:00
abstract::Since the identification of approximately 25,000 proteins from the draft human genome assembly in 2001, estimates of the total have oscillated between 30,000 and 70,000. The recently announced genome closure has not generated a consensus gene count despite this being a key parameter for many areas of biology including...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200300700
更新日期:2004-06-01 00:00:00
abstract::Liquid Chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS) is a powerful analytical technique for the identification and mass analysis of complex protein mixtures. Here, we present a combination of methods developed for the extensive/deep proteomic analysis of purified ribosome/mRNA particles assembled i...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400628
更新日期:2015-07-01 00:00:00
abstract::Scanning MS by MALDI MS imaging (MALDI-MSI) creates large volumetric global datasets that describe the location and identity of ions registered at each sampling location. While thousands of ion peaks are recorded in a typical whole-tissue analysis, only a fraction of these measured molecules are purposefully scrutiniz...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300431
更新日期:2014-04-01 00:00:00
abstract::Spatial organisation of proteins according to their function plays an important role in the specificity of their molecular interactions. Emerging proteomics methods seek to assign proteins to sub-cellular locations by partial separation of organelles and computational analysis of protein abundance distributions among ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000359
更新日期:2010-12-01 00:00:00
abstract::Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human thyroid cells were investigated ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200070
更新日期:2012-08-01 00:00:00
abstract::Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000548
更新日期:2011-03-01 00:00:00
abstract::DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800298
更新日期:2008-12-01 00:00:00
abstract::Pathogenic mycobacteria persist and replicate within phagosomes of host phagocytes by inhibiting phagosome maturation at an early endosome stage. The molecular basis for this behavior is not understood. To identify proteins of Mycobacterium tuberculosis unique to the intraphagosomal phase, mycobacteria were purified f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500547
更新日期:2006-04-01 00:00:00
abstract::Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800935
更新日期:2009-05-01 00:00:00
abstract::Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and sub-tropical oceans. It is unique in that it exclusively fixes N(2) at daytime, although it belongs to the non-heterocystous filamentous segment of the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000382
更新日期:2011-02-01 00:00:00
abstract::Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800009
更新日期:2008-07-01 00:00:00
abstract::This is the first broad proteomic description of Gluconacetobacter diazotrophicus, an endophytic bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in tropical regions. Proteomic coverage of G. diazotrophicus PAL5 was obtained by two independent approaches: 2-DE followed by MA...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700912
更新日期:2008-04-01 00:00:00
abstract::Despite increasing evidence of behavioural manipulation of their vectors by pathogens, the underlying mechanisms causing infected vectors to act in ways that benefit pathogen transmission remain enigmatic in most cases. Here, 2-D DIGE coupled with MS were employed to analyse and compare the head proteome of mosquitoes...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200601021
更新日期:2007-06-01 00:00:00
abstract::In shotgun proteomics, proteins can be fractionated by 1-D gel electrophoresis and digested into peptides, followed by liquid chromatography to separate the peptide mixture. Mass spectrometry generates hundreds of thousands of tandem mass spectra from these fractions, and proteins are identified by database searching....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500318
更新日期:2006-02-01 00:00:00
abstract::The solubilization of a large array of proteins before electrophoresis itself is a very critical point for proteomic analyses. We compared the efficiency of several different solubilization buffers. From this work, we defined a very efficient solubilization buffer, including two chaotropes, two reducing agents (R2), t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300450
更新日期:2003-07-01 00:00:00
abstract::After hind limb suspension, a remodeling of postural muscle phenotype is observed. This remodeling results in a shift of muscle profile from slow-oxidative to fast-glycolytic. These metabolic changes and fiber type shift increase muscle fatigability. Acetyl-L-carnitine (ALCAR) influences the skeletal muscle phenotype ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701176
更新日期:2008-09-01 00:00:00
abstract::Progress in MS-based methods for veterinary research and diagnostics is lagging behind compared to the human research, and proteome data of domestic animals is still not well represented in open source data repositories. This is particularly true for the equine species. Here we present a first Equine PeptideAtlas enco...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300398
更新日期:2014-03-01 00:00:00
abstract::Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, inclu...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700556
更新日期:2008-04-01 00:00:00
abstract::The characterization of peptides bound to human leukocyte antigen (HLA) class I is of fundamental importance for understanding CD8+ T cell-driven immunological processes and for the development of immunomodulatory therapeutic strategies. However, until now, the mass spectrometric analysis of HLA-bound peptides has typ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500445
更新日期:2016-05-01 00:00:00
abstract::Methyl parathion (MP) is a widely used organophosphorus pesticide, which has been related to a broad spectrum of toxic effects on environmental organisms. The present study investigated the changes in the protein profile of enriched membrane fraction from zebrafish (Danio rerio) brain exposed to three concentrations (...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100084
更新日期:2011-09-01 00:00:00
abstract::In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400785
更新日期:2004-09-01 00:00:00
abstract::In this study, various proteomics-based methods were utilized to examine the 14-3-3 protein family in Arabidopsis thaliana. A protein extract was prepared from an Arabidopsis hypocotyl suspension culture and analyzed by two-dimensional gel electrophoresis and immunoblotting with a 14-3-3 monoclonal antibody that recog...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500729
更新日期:2006-05-01 00:00:00
abstract::Gliadins and glutenins are the main protein fractions present in wheat gluten. They are responsible for technological and nutritional quality of wheat based products. In particular, glutenins are mainly responsible for dough visco-elastic properties, whereas gliadins confer extensibility to dough and are the most impo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500236
更新日期:2006-03-01 00:00:00
abstract::Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt-tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV-irradiated Agropyron elongatum (...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700569
更新日期:2008-04-01 00:00:00