Abstract:
:Production of membrane proteins (MPs) is a challenging task as their hydrophobic nature and their specific requirements in cellular expression systems frequently prevent an efficient synthesis. Cell-free (CF) expression systems have been developed in recent times as promising tools by offering completely new approaches to synthesize MPs directly into artificial hydrophobic environments. A considerable variety of CF produced MPs has been characterized by functional and structural approaches and the high success rates and the rapidly accumulating data on quality and expression efficiencies increasingly attract attention. In addition, CF expression is a highly dynamic and versatile technique and new modifications for improved performance as well as for extended applications for the labeling, throughput expression and proteomic analysis of MPs are rapidly emerging.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Schwarz D,Dötsch V,Bernhard Fdoi
10.1002/pmic.200800171subject
Has Abstractpub_date
2008-10-01 00:00:00pages
3933-46issue
19eissn
1615-9853issn
1615-9861journal_volume
8pub_type
杂志文章,评审相关文献
PROTEOMICS文献大全abstract::The skin provides protection against environmental stress. However, intrinsic and extrinsic aging causes significant alteration to skin structure and components, which subsequently impairs molecular characteristics and biochemical processes. Here, we have conducted an immunohistological investigation and established t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600141
更新日期:2016-10-01 00:00:00
abstract::"Liquid biopsies" have received attention as a complementary tool for traditional tissue biopsies that may enhance the spectrum of analysis for tumor-derived factors. One such factor gaining prominence in the liquid biopsy field is extracellular vesicles (EVs), membrane-bound nanovesicles which are secreted by cells i...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800155
更新日期:2019-01-01 00:00:00
abstract::Despite affecting up to 70% of HIV-positive patients and being the leading cause of dementia in patients under 40 years, the molecular mechanisms involved in the onset of HIV-associated neurocognitive disorders (HAND) are not well understood. To address this, we performed SILAC-based quantitative proteomic analysis on...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600236
更新日期:2017-03-01 00:00:00
abstract::Induced sputum is a readily accessible biological fluid whose composition may alter as a consequence of disease. To date, however, the proteins that routinely populate this biofluid are largely unknown, in part due to the technical difficulties in processing such mucin-rich samples. To provide a catalogue of sputum pr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600011
更新日期:2006-08-01 00:00:00
abstract::Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400610
更新日期:2015-09-01 00:00:00
abstract::The ascomycete Botrytis cinerea is a phytopathogenic fungus infecting and causing significant yield losses in a number of crops. Moreover, in the last few years, B. cinerea has been adopted as an important model system in molecular phytopathology. In spite of these contributions, the molecular basis of the infection c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800540
更新日期:2009-05-01 00:00:00
abstract::The ubiquitin-proteasome system allows the targeted degradation of proteins and plays a critical role in the regulation of many cellular processes. Proteasome inhibition is a recent antitumor therapeutic strategy and bortezomib was the first proteasome inhibitor approved for clinical use. In this study, we used the NB...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200233
更新日期:2013-01-01 00:00:00
abstract::In recent years, proteomics research has gained importance due to increasingly powerful techniques in protein purification, mass spectrometry and identification, and due to the development of extensive protein and DNA databases from various organisms. Nevertheless, current identification methods from spectrometric dat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300402
更新日期:2003-06-01 00:00:00
abstract::We describe a new approach for the characterization of a digested protein complex with quantitative aspects. Accurate masses of tryptic peptides in the digested complex were acquired by nano-liquid chromatography Fourier transform-ion cyclotron resonance mass spectrometry (MS). The conditions of the electrospray ion s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300812
更新日期:2004-09-01 00:00:00
abstract::The protein components of urine are useful indicators of renal function and human health in general. Urine samples are easily attainable making them ideal substrates for biomarker research. Analysis of the urine proteome however, has been hindered by the great variability of the urine specimens, and the presence of va...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500671
更新日期:2006-08-01 00:00:00
abstract::In the present study, changes in protein synthesis patterns after salt shock visualized by 35S-methionine labeling and the changed protein composition in salt-acclimated cells of the cyanobacterium Synechocystis sp. strain PCC 6803 were analyzed by a combination of 2-DE for protein separation and PMF for protein ident...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500538
更新日期:2006-05-01 00:00:00
abstract::Employing classical two-dimensional electrophoresis (2-DE), amino acid sequencing and immunoblot analysis, we examine for the first time the effect of ozone, a highly notorious environmental pollutant, on rice seedling proteins. Drastic visible necrotic damage to leaf by ozone and consequent increase in ascorbate pero...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200208)2:8<947::AID-PROT947>3.0.
更新日期:2002-08-01 00:00:00
abstract::Daphnia pulex is a keystone species for aquatic habitats and an ecological/evolution model organism. Although significant progress has been made on characterizing its genome, the D. pulex proteome remains largely uncharacterized partially due to abnormally high protein degradation during homogenization and emphasis on...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700460
更新日期:2018-08-01 00:00:00
abstract::Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500510
更新日期:2016-06-01 00:00:00
abstract::Egr1 (Zif268) is an immediate early gene encoding an inducible transcription factor involved in synaptic plasticity and several forms of memory in rodents. Using 2-DE and MS, we compared proteomes of hippocampal subregions and cortex in Egr1-deficient and wild-type littermates. Two significant differences were identif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600513
更新日期:2007-01-01 00:00:00
abstract::By means of a degradomic approach applying proteomic techniques, we previously suggested that apolipoprotein E (apoE) is a substrate of matrix metalloproteinase-14 (MMP-14). Here we confirm that apoE is, in fact, a substrate of MMP-14 and also of MMP-7 and MMP-2 to a lesser extent. The 34 kDa apoE protein was initiall...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700487
更新日期:2008-07-01 00:00:00
abstract::A novel type of isobaric internal peptide standard for quantitative proteomics is described. The standard is a synthetic peptide derived from the target peptide by positional permutation of two amino acids. This type of internal standard is denominated minimally permutated peptide analog (MIPA). MIPA can be differenti...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900695
更新日期:2010-04-01 00:00:00
abstract::Four rhoptry proteins (ROP) of Toxoplasma gondii previously identified with mAb have been affinity purified and analyzed by MS; the data obtained allowed the genomic sequences to be assigned to these proteins. As previously suggested for some of them by antibody crossreactivity, these proteins were shown to belong to ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600187
更新日期:2006-11-01 00:00:00
abstract::Citrulline (Cit) actions on muscle metabolism remain unclear. Those latter were investigated using a proteomic approach on Tibialis muscles from male Sprague-Dawley rats. At 23 months of age, rats were either fed ad libitum (AL group) or subjected to dietary restriction for 12 weeks. At the end of the restriction peri...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200262
更新日期:2013-07-01 00:00:00
abstract::Quantitative proteomics technology based on isobaric tags is playing an important role in proteomic investigation. In this paper, we present an automated software, named IQuant, which integrates a postprocessing tool of protein identification and advanced statistical algorithms to process the MS/MS signals generated f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300361
更新日期:2014-10-01 00:00:00
abstract::Blood plasma is the most complex human-derived proteome, containing other tissue proteomes as subsets. This proteome has only been partially characterized due to the extremely wide dynamic range of the plasma proteins of more than ten orders of magnitude. Thus, the reduction in sample complexity prior to mass spectrom...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700166
更新日期:2007-12-01 00:00:00
abstract::The ectopic expression of the gene SEL1L in the human breast carcinoma cell line MCF-7 resulted in a reduction of the aggressive behaviour of these cells in vitro. In addition, in vivo analysis on a series of primary breast carcinomas revealed an association between the SEL1L protein levels and the patient's overall s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401283
更新日期:2005-06-01 00:00:00
abstract::Oviductal fluid (ODF) proteins modulate and support reproductive processes in the oviduct. In the present study, proteins involved in the biological events that precede fertilization have been identified in the rabbit ODF proteome, isolated from the ampulla and isthmus of the oviduct at different time points within 8 ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800319
更新日期:2019-03-01 00:00:00
abstract::Surface-enhanced laser desorption/ionization (SELDI) time of flight (TOF) is a mass spectrometry technology for measuring the composition of a sampled protein mixture. A mass spectrum contains peaks corresponding to proteins in the sample. The peak areas are proportional to the measured concentrations of the correspon...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600181
更新日期:2006-10-01 00:00:00
abstract::Compared with N-linked glycosylation, the analysis of O-GalNAc glycosylation is extremely challenging due to the high structure diversity of glycans and lack of glycosidases to release O-GalNAc glycans. In this work, a glycoform simplification strategy by combining HILIC enrichment with chemical de-sialylation to char...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800042
更新日期:2018-09-01 00:00:00
abstract::With recent progress in the analysis of the salivary proteome, the number of salivary proteins identified has increased dramatically. However, the physiological functions of many of the newly discovered proteins remain unclear. Closely related to the study of a protein's function is the identification of its interacti...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100665
更新日期:2012-11-01 00:00:00
abstract::A diverse range of proteins are modified by the post-translational covalent attachment of a glycosyl-phosphatidylinositol (GPI) membrane anchor. Hydropathy plots and other computer algorithms can be used to predict the presence of a GPI anchor attachment signal in the nascent polypeptide chain. However, the presence o...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/1615-9861(200106)1:6<748::AID-PROT748>3.0.
更新日期:2001-06-01 00:00:00
abstract::Human galectin-3 (hGal-3) is a mammalian lectin involved in regulation of RNA splicing, apoptosis, cell differentiation, and proliferation. Multimerized extracellular hGal-3 is thought to crosslink cells by binding to glycoproteins and glycosylated cancer antigens on the cell surface or extracellular matrix. Fluoresce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900581
更新日期:2010-05-01 00:00:00
abstract::On 23 and 24 July, 2007, the Centre for Economic and Social Aspects of Genomics (CESAGen) held its first sociomics workshop at the Wellcome Trust Genome Campus, Hinxton, UK. The topic was transformation of knowledge production. Participants included social scientists together with those working on different elements o...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200700929
更新日期:2008-01-01 00:00:00
abstract::The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this st...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600417
更新日期:2017-09-01 00:00:00