Shotgun proteomic analysis of human-induced sputum.

abstract：:The endothelium is a single layer of cells lining the inside face of all blood vessels. It constitutes a major metabolic organ which is critically involved in the generation and the regulation of multiple physiological and pathological processes such as coagulation, hemostasis, inflammation, atherosclerosis, angiogene...

journal_title：Proteomics

authors： Bruneel A,Labas V,Mailloux A,Sharma S,Vinh J,Vaubourdolle M,Baudin B

更新日期：2003-05-01 00:00:00

abstract：:Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and sub-tropical oceans. It is unique in that it exclusively fixes N(2) at daytime, although it belongs to the non-heterocystous filamentous segment of the...

journal_title：Proteomics

authors： Sandh G,Ran L,Xu L,Sundqvist G,Bulone V,Bergman B

更新日期：2011-02-01 00:00:00

abstract：:Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...

journal_title：Proteomics

authors： Corrigan L,Jefferies C,Clive Lee T,Daly J

更新日期：2011-08-01 00:00:00

abstract：:Abnormal phosphorylation contributes to the formation of neurofibrillary tangles in Alzheimer's disease (AD), but may play other signaling roles during AD pathogenesis. In this study, we employed IMAC followed by LC-MS/MS to identify phosphopeptides from eight individual AD and eight age-matched control postmortem hum...

journal_title：Proteomics

authors： Dammer EB,Lee AK,Duong DM,Gearing M,Lah JJ,Levey AI,Seyfried NT

更新日期：2015-01-01 00:00:00

abstract：:The current treatment for glioblastoma includes temozolomide (TMZ) chemotherapy, yet the mechanism of action of TMZ is not thoroughly understood. Here, we investigated the TMZ-induced changes in the proteome of the glioma-derived cell line (U251) by 2D DIGE. We found 95 protein spots to be significantly altered in the...

journal_title：Proteomics

authors： Kumar DM,Patil V,Ramachandran B,Nila MV,Dharmalingam K,Somasundaram K

更新日期：2013-07-01 00:00:00

abstract：:The aims of the study were to: (i) identify differentially regulated proteins in cerebrospinal fluid (CSF) between multiple sclerosis (MS) patients and non-MS controls; (ii) examine the effect of matching the CSF samples on either total protein amount or volume, and compare four protein normalization strategies for CS...

journal_title：Proteomics

authors： Opsahl JA,Vaudel M,Guldbrandsen A,Aasebø E,Van Pesch V,Franciotta D,Myhr KM,Barsnes H,Berle M,Torkildsen Ø,Kroksveen AC,Berven FS

更新日期：2016-04-01 00:00:00

abstract：:In this study, a method, based on dual column affinity chromatography hyphenated to isotope dilution inductively coupled plasma-quadrupole MS, was developed for selenium determination in selenoprotein P, glutathione peroxidase, and selenoalbumin in human serum samples from a group of healthy volunteers (n=399). Method...

journal_title：Proteomics

authors： Letsiou S,Lu Y,Nomikos T,Antonopoulou S,Panagiotakos D,Pitsavos C,Stefanadis C,Pergantis SA

更新日期：2010-10-01 00:00:00

abstract：:The computational simulation of complete proteomic data sets and their utility to validate detection and interpretation algorithms, to aid in the design of experiments and to assess protein and peptide false discovery rates is presented. The simulation software has been developed for emulating data originating from da...

journal_title：Proteomics

authors： Geromanos SJ,Hughes C,Golick D,Ciavarini S,Gorenstein MV,Richardson K,Hoyes JB,Vissers JP,Langridge JI

更新日期：2011-03-01 00:00:00

abstract：:We here present the jmzReader library: a collection of Java application programming interfaces (APIs) to parse the most commonly used peak list and XML-based mass spectrometry (MS) data formats: DTA, MS2, MGF, PKL, mzXML, mzData, and mzML (based on the already existing API jmzML). The library is optimized to be used i...

journal_title：Proteomics

authors： Griss J,Reisinger F,Hermjakob H,Vizcaíno JA

更新日期：2012-03-01 00:00:00

abstract：:The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was...

journal_title：Proteomics

authors： Sinnige JC,de Been M,Zhou M,Bonten MJ,Willems RJ,Top J

更新日期：2015-11-01 00:00:00

abstract：:Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...

journal_title：Proteomics

authors： ten Have S,Boulon S,Ahmad Y,Lamond AI

更新日期：2011-03-01 00:00:00

abstract：:Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct...

journal_title：Proteomics

authors： Beaudette P,Popp O,Dittmar G

更新日期：2016-01-01 00:00:00

abstract：:We monitored rates of growth, ATP-synthesis, respiration, and death to assess the sensitivity of the model organism Pseudomonas putida KT2440 to methyl tert-butyl ether (MTBE), and its degree of toxicity. The physiological data obtained suggested that the energy conservation system was the most sensitive site. However...

journal_title：Proteomics

authors： Krayl M,Benndorf D,Loffhagen N,Babel W

更新日期：2003-08-01 00:00:00

abstract：:A simple and rapid method for characterizing hydrophobic integral membrane proteins and its utility for membrane proteomics using microcapillary liquid chromatography coupled on-line with tandem mass spectrometry (microLC-MS/MS) is described. The present technique does not rely on the use of detergents, strong organic...

journal_title：Proteomics

authors： Blonder J,Conrads TP,Yu LR,Terunuma A,Janini GM,Issaq HJ,Vogel JC,Veenstra TD

更新日期：2004-01-01 00:00:00

abstract：:Sample preparation is a critical step in the proteomic workflow. Numerous different approaches are used, tailored to the type of sample, the aims of the experiment, analytical method, and to an extent, user preference. This has resulted in large variation in reported protein abundances. In this study, the complementar...

journal_title：Proteomics

authors： Howard M,Achour B,Al-Majdoub Z,Rostami-Hodjegan A,Barber J

更新日期：2018-12-01 00:00:00

abstract：:In theory, proteases with broad cleavage specificity could be applied to digest protein samples to improve the phosphoproteomic analysis coverage. However, in practice this approach is seldom employed. This is because the identification of phosphopeptides without enzyme specificity by conventional database search stra...

journal_title：Proteomics

authors： Dong M,Ye M,Cheng K,Dong J,Zhu J,Qin H,Bian Y,Zou H

更新日期：2014-11-01 00:00:00

abstract：:Daphnia pulex is a keystone species for aquatic habitats and an ecological/evolution model organism. Although significant progress has been made on characterizing its genome, the D. pulex proteome remains largely uncharacterized partially due to abnormally high protein degradation during homogenization and emphasis on...

journal_title：Proteomics

authors： Steevensz A,Gombar R,Vergilino R,Cristescu ME,Vacratsis PO

更新日期：2018-08-01 00:00:00

abstract：:Plant tissues contain large amounts of secondary compounds that significantly interfere with protein extraction and 2DE analysis. Thus, sample preparation is a crucial step prior to 2DE in plant proteomics. This tutorial highlights the guidelines that need to be followed to perform an adequate total protein extraction...

journal_title：Proteomics

authors： Wu X,Gong F,Wang W

更新日期：2014-03-01 00:00:00

abstract：:Biological membranes are compartmentalized into microdomains that exhibit particular lipid and protein compositions. Membrane microdomains, such as tetraspanin-enriched microdomains and lipid rafts, have been suggested to play a role in a variety of physiological and pathological processes. Therefore, the characteriza...

journal_title：Proteomics

authors： Le Naour F,André M,Boucheix C,Rubinstein E

更新日期：2006-12-01 00:00:00

abstract：:It becomes increasingly clear that separation of pure cell populations provides a uniquely sensitive and accurate approach to protein profiling in biological systems and opens up a new area for proteomic analysis. The method we described could simultaneously isolate population of hepatocytes (HCs), hepatic stellate ce...

journal_title：Proteomics

authors： Liu W,Hou Y,Chen H,Wei H,Lin W,Li J,Zhang M,He F,Jiang Y

更新日期：2011-09-01 00:00:00

abstract：:Leishmaniasis is a zoonotic disease caused by the species of the genus Leishmania, flagellated protozoa that multiply inside mammalian macrophages and are transmitted by the bite of the sandfly. The disease is widespread and due to the lack of fully effective treatment and vaccination the search for new drugs and immu...

journal_title：Proteomics

authors： Dea-Ayuela MA,Rama-Iñiguez S,Bolás-Fernández F

更新日期：2006-07-01 00:00:00

abstract：:Proteolysis affects every protein at some point in its life cycle. Many biomarkers of disease or cancer are stable proteolytic fragments in biological fluids. There is great interest and a challenge in proteolytically modified protein study to identify physiologic protease-substrate relationships and find potential bi...

journal_title：Proteomics

authors： Shen C,Yu Y,Li H,Yan G,Liu M,Shen H,Yang P

更新日期：2012-06-01 00:00:00

abstract：:Plants exhibit resistance against incompatible pathogens, via localized and systemic responses as part of an integrated defense mechanism. To study the compatible and incompatible interactions between rice and bacteria, a proteomic approach was applied. Rice cv. Java 14 seedlings were inoculated with compatible (Xo743...

journal_title：Proteomics

authors： Mahmood T,Jan A,Kakishima M,Komatsu S

更新日期：2006-11-01 00:00:00

abstract：:During early stages of embryo development, the brain cavity is filled with embryonic cerebrospinal fluid (E-CSF), a complex fluid containing different protein fractions that contributes to the regulation of the survival, proliferation and neurogenesis of the neuroectodermal stem cells. Using 2-DE, protein sequencing a...

journal_title：Proteomics

authors： Parada C,Gato A,Aparicio M,Bueno D

更新日期：2006-01-01 00:00:00

abstract：:T helper cells (Th) are divided into Th1 and Th2 subsets based upon their cytokine profiles and function. Naïve Th cells differentiate into Th1 and Th2 subsets depending on the antigens, costimulatory molecules, and cytokines they encounter. Cytokine interleukin (IL)-12 enhances the generation of Th1 lymphocytes and i...

journal_title：Proteomics

authors： Filén JJ,Nyman TA,Korhonen J,Goodlett DR,Lahesmaa R

更新日期：2005-12-01 00:00:00

abstract：:Realistic in vitro models are critical in the drug development process. In this study, a novel in vitro platform is employed to assess drug penetration and metabolism. This platform, which utilizes a 3D printed fluidic device, allows for dynamic dosing of three dimensional cell cultures, also known as spheroids. The p...

journal_title：Proteomics

authors： LaBonia GJ,Lockwood SY,Heller AA,Spence DM,Hummon AB

更新日期：2016-06-01 00:00:00

abstract：:Immune proteomics is an increasingly powerful tool for the investigation of the adaptive immune response to natural encounters between micro-organisms and their hosts. The versatile species Staphylococcus aureus serves to illustrate how these techniques can be employed to appreciate the complexity and diversity of the...

journal_title：Proteomics

authors： Bröker BM,van Belkum A

更新日期：2011-08-01 00:00:00

abstract：:The PIK3CA gene encodes for the p110 alpha isoform of PI3 kinase and is one of the most frequently mutated oncogenes in human cancers. However, the mechanisms by which PIK3CA mutations activate cell signaling are not fully understood. Here we used a phosphoproteomic approach to compare differential phosphorylation pat...

journal_title：Proteomics

authors： Blair BG,Wu X,Zahari MS,Mohseni M,Cidado J,Wong HY,Beaver JA,Cochran RL,Zabransky DJ,Croessmann S,Chu D,Toro PV,Cravero K,Pandey A,Park BH

更新日期：2015-01-01 00:00:00

abstract：:The HUPO Brain Proteome Project (HUPO BPP) held its 12th workshop in Toronto on 26 September 2009 prior to the HUPO VIII World Congress. The principal aim of this project is to obtain a better understanding of neurodiseases and ageing, with the ultimate objective of discovering prognostic and diagnostic biomarkers, in...

journal_title：Proteomics

authors： Gröttrup B,Eisenacher M,Stephan C,Marcus K,Lee B,Meyer HE,Park YM

更新日期：2010-06-01 00:00:00

abstract：:Mass spectrometry-based proteomics is used to gain insight into the abundance and subcellular localization of cellular signaling components, the composition of molecular complexes and the regulation of signaling pathways. Multicellular organisms have evolved signaling networks and fast responses to stimuli that can be...

journal_title：Proteomics

authors： Kaufmann K,Smaczniak C,de Vries S,Angenent GC,Karlova R

更新日期：2011-02-01 00:00:00