Abstract:
:The aims of the study were to: (i) identify differentially regulated proteins in cerebrospinal fluid (CSF) between multiple sclerosis (MS) patients and non-MS controls; (ii) examine the effect of matching the CSF samples on either total protein amount or volume, and compare four protein normalization strategies for CSF protein quantification. CSF from MS patients (n = 37) and controls (n = 64), consisting of other noninflammatory neurological diseases (n = 50) and non neurological spinal anesthetic subjects (n = 14), were analyzed using label-free proteomics, quantifying almost 800 proteins. In total, 122 proteins were significantly regulated (p < 0.05), where 77 proteins had p-value <0.01 or AUC value >0.75. Hierarchical clustering indicated that there were two main groups of MS patients, those with increased levels of inflammatory response proteins and decreased levels of proteins involved in neuronal tissue development (n = 30), and those with normal protein levels for both of these protein groups (n = 7). The main subgroup of controls clustering with the MS patients showing increased inflammation and decreased neuronal tissue development were patients suffering from chronic fatigue. Our data indicate that the preferable way to quantify proteins in CSF is to first match the samples on total protein amount and then normalize the data based on the median intensities, preferably from the CNS-enriched proteins.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Opsahl JA,Vaudel M,Guldbrandsen A,Aasebø E,Van Pesch V,Franciotta D,Myhr KM,Barsnes H,Berle M,Torkildsen Ø,Kroksveen AC,Berven FSdoi
10.1002/pmic.201500284subject
Has Abstractpub_date
2016-04-01 00:00:00pages
1154-65issue
7eissn
1615-9853issn
1615-9861journal_volume
16pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Mitochondria consist of four compartments, outer membrane, intermembrane space, inner membrane, and matrix; each harboring specific functions and structures. In this study, we used LC-MS/MS to characterize the protein composition of Trypanosoma brucei mitochondrial (mt) membranes, which were enriched by different bioc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900354
更新日期:2009-12-01 00:00:00
abstract::The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis proce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100313
更新日期:2012-01-01 00:00:00
abstract::In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400785
更新日期:2004-09-01 00:00:00
abstract::Simpson-Golabi-Behmel syndrome (SGBS) is an X-linked condition shown to be the result of deletions of the glypican-3 (GPC3) gene. GPC3 is a proteoglycan localized to the cell membrane via a glycosylphosphatidyl-inositol (GPI) anchor. To further elucidate the GPC3 function(s), we have screened various cell lines for pr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600654
更新日期:2007-06-01 00:00:00
abstract::Recently (Cytometry 2003, 56A, 71-80), we reported that direct cell-to-cell contact is required for stimulating proliferation of bystander rat liver cells (WB-F344) cocultured with irradiated cells, and neither functional gap junction intercellular communication nor long-range extracellular factors appear to be involv...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600948
更新日期:2007-06-01 00:00:00
abstract::Long-lived conifer trees depend on both constitutive and induced defenses for resistance against a myriad of potential pathogens and herbivores. In species of spruce (Picea spp.), several of the late events of pathogen-, insect-, or elicitor-induced defense responses have previously been characterized at the anatomica...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800252
更新日期:2009-01-01 00:00:00
abstract::Many proteomic studies focus on quantitative aspects, using different stable isotope labeling techniques that require specialized software to analyze the generated data. Here we present jTraqX, an easy-to-use tool for processing and visualizing protein quantification data. jTraqX is platform independent and is compati...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900374
更新日期:2010-03-01 00:00:00
abstract::The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900836
更新日期:2010-05-01 00:00:00
abstract::The lipid droplet (LD) is an organelle with vital functions found in nearly all organisms. LD proteomic research has provided fundamentally important insights into this organelle's functions. The review provides a summary of LD proteomic studies conducted across diverse organisms and cell and tissue types. The accumul...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201700223
更新日期:2019-05-01 00:00:00
abstract::The identification of specific protein markers for colorectal cancer would provide the basis for early diagnosis and detection, as well as clues for understanding the molecular mechanisms governing cancer progression. In this report, we describe the proteomic analysis of the samples of colorectal cancer corresponding ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401196
更新日期:2005-07-01 00:00:00
abstract::We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HP...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500635
更新日期:2006-04-01 00:00:00
abstract::Secreted proteins play a pivotal role in cellular functions. To better understand malignant behavior, we adapted stable isotopic labeling with amino acids in cell culture technology to identify and quantify proteins differentially released into the extracellular media by a pair of normal and malignant breast-cancer ce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700957
更新日期:2009-01-01 00:00:00
abstract::Chinese tarantula, Chilobrachys jingzhao is one of the most venomous spiders in southern China and its venom is a mixture of various compounds with diversified biological activities. The proteome of C. jingzhao venom was analyzed by proteomic techniques. Proteins with molecular weight of over 10 kDa, indicated by gel-...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600785
更新日期:2007-06-01 00:00:00
abstract::Identification and characterization of biomarkers in body fluids such as serum or urine serve as a basis for early detection of diseases, particularly of cancer. Performing 2-DE with subsequent MS analyses, conventional immunoblotting and immunohistochemistry we identified two proteins, orosomucoid (ORM) and human zin...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200402005
更新日期:2005-11-01 00:00:00
abstract::The determination of possible biomarkers in nasal secretion of healthy subjects can have a role in early diagnosis of diseases such as rhinosinusitis. For this purpose, nasal lavage fluids (NLFs) from ten volunteers, collected before and after they had been submitted to nasal provocations, were investigated. Separatio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401172
更新日期:2005-07-01 00:00:00
abstract::The recombinant plasmid pASK18 carries a Streptomyces DNA fragment which includes an open reading frame, designated psfS (putative sigma factor, Streptomyces), as its putative product showed a high degree of similarity with RNA polymerase sigma factors. Previous results showed that PsfS causes transcription initiation...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300403
更新日期:2003-06-01 00:00:00
abstract::CID has become a routine method for fragmentation of peptides in shotgun proteomics, whereas electron transfer dissociation (ETD) has been described as a preferred method for peptides carrying labile PTMs. Though both of these fragmentation techniques have their obvious advantages, they also have their own drawbacks. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000547
更新日期:2011-06-01 00:00:00
abstract::Canonical isoforms in different databases have been defined as the most prevalent, most conserved, most expressed, longest, or the one with the clearest description of domains or posttranslational modifications. In this article, we revisit these definitions of canonical isoforms based on functional genomics and proteo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400170
更新日期:2014-12-01 00:00:00
abstract::Aedes aegypti females ingest sugar or blood to obtain the nutrients needed to maintain cellular homeostasis. During human blood ingestion, female mosquitoes may transmit different viruses such as dengue, yellow fever and, more recently, zika and chikungunya. Here, we report changes in protein expression in the heads o...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600126
更新日期:2016-10-01 00:00:00
abstract::Ischemia/reperfusion (I/R) injury is a major consequence of a cardiovascular intervention. The study of changes of the left and right ventricle proteomes from hearts subjected to I/R may be a key to revealing the pathological mechanisms underlying I/R-induced heart contractile dysfunction. Isolated rat hearts were per...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100604
更新日期:2012-08-01 00:00:00
abstract::Data-independent acquisition (DIA) offers several advantages over data-dependent acquisition (DDA) schemes for characterizing complex protein digests analyzed by LC-MS/MS. In contrast to the sequential detection, selection, and analysis of individual ions during DDA, DIA systematically parallelizes the fragmentation o...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201400323
更新日期:2015-03-01 00:00:00
abstract::On 23 and 24 July, 2007, the Centre for Economic and Social Aspects of Genomics (CESAGen) held its first sociomics workshop at the Wellcome Trust Genome Campus, Hinxton, UK. The topic was transformation of knowledge production. Participants included social scientists together with those working on different elements o...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200700929
更新日期:2008-01-01 00:00:00
abstract::The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500138
更新日期:2015-11-01 00:00:00
abstract::White spot syndrome virus (WSSV) is a pathogen that causes considerable mortality of the farmed shrimp, Penaeus monodon. Candidate 'vaccines', WSSV envelope protein VP28 and formalin-inactivated WSSV, can provide short-lived protection against the virus. In this study, P. monodon was orally intubated with the aforemen...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300405
更新日期:2014-07-01 00:00:00
abstract::Previous studies have found that some first onset schizophrenia patients show signs of impaired insulin signaling. Also, epidemiological studies have shown that periods of suboptimal nutrition including protein deficiencies during pregnancy can lead to increased incidence of metabolic conditions and psychiatric disord...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200376
更新日期:2012-12-01 00:00:00
abstract::Systematic detection of low-abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently comm...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401228
更新日期:2005-08-01 00:00:00
abstract::A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000218
更新日期:2021-01-01 00:00:00
abstract::Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt-tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV-irradiated Agropyron elongatum (...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700569
更新日期:2008-04-01 00:00:00
abstract::Changing smooth muscle phenotype and abnormal cell proliferation are important features of vascular pathology, including the failure of saphenous vein bypass grafts. We have characterised and mapped protein expression in human saphenous vein medial smooth muscle, using two-dimensional (2-D) polyacrylamide gel electrop...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200111)1:11<1405::AID-PROT1405>3
更新日期:2001-11-01 00:00:00
abstract::While metastasis is the primary cause of colorectal cancer (CRC) mortality, the molecular mechanisms underpinning it remains elusive. Metastasis is propagated through driver oncogene/suppressor gene mutations, accompanied by passenger mutations and underlying genomic instability. To understand cancer biology, a unifyi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900026
更新日期:2019-11-01 00:00:00