Abstract:
:We monitored rates of growth, ATP-synthesis, respiration, and death to assess the sensitivity of the model organism Pseudomonas putida KT2440 to methyl tert-butyl ether (MTBE), and its degree of toxicity. The physiological data obtained suggested that the energy conservation system was the most sensitive site. However, with the help of proteomic analysis we obtained further information and deeper insight into the molecular mechanisms involved. This analysis indicated that sensitivity involves oxidative stress since alkylhydroperoxide reductase C (AhpC) and two superoxide dismutases (SodM, SodF) were amplified in the presence of MTBE. Thus, proteomics has major advantages in ecotoxicological investigations where the aims include elucidation of the molecular mechanisms as well as characterization of the ecostress and the potency of the stressor(s).
journal_name
Proteomicsjournal_title
Proteomicsauthors
Krayl M,Benndorf D,Loffhagen N,Babel Wdoi
10.1002/pmic.200300477subject
Has Abstractpub_date
2003-08-01 00:00:00pages
1544-52issue
8eissn
1615-9853issn
1615-9861journal_volume
3pub_type
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