Characterization of heat shock protein 27 phosphorylation sites in renal cell carcinoma.

abstract：:Plants exhibit resistance against incompatible pathogens, via localized and systemic responses as part of an integrated defense mechanism. To study the compatible and incompatible interactions between rice and bacteria, a proteomic approach was applied. Rice cv. Java 14 seedlings were inoculated with compatible (Xo743...

journal_title：Proteomics

authors： Mahmood T,Jan A,Kakishima M,Komatsu S

更新日期：2006-11-01 00:00:00

abstract：:Selective activation of T helper subsets 1 (Th1) and 2 (Th2) plays a crucial role in different pathological conditions. Th1 cell response is involved in pathogenesis of autoimmune diseases, such as type II diabetes and multiple sclerosis, and Th2 cell response in pathogenesis of allergy and asthma. Cytokine interleuki...

journal_title：Proteomics

authors： Rosengren AT,Nyman TA,Lahesmaa R

更新日期：2005-08-01 00:00:00

abstract：:Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +8...

journal_title：Proteomics

authors： Grozav AG,Willard BB,Kozuki T,Chikamori K,Micluta MA,Petrescu AJ,Kinter M,Ganapathi R,Ganapathi MK

更新日期：2011-03-01 00:00:00

abstract：:Recent advances in two-dimensional electrophoresis (2-DE) such as fluorescent 2-D differential gel electrophoresis (2-D DIGE) has made it possible to detect and quantitate the critical changes involved in disease pathogenesis. We have previously identified novel proteins with altered expression in primary colorectal c...

journal_title：Proteomics

authors： Nishimori T,Tomonaga T,Matsushita K,Oh-Ishi M,Kodera Y,Maeda T,Nomura F,Matsubara H,Shimada H,Ochiai T

更新日期：2006-02-01 00:00:00

abstract：:Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage ...

journal_title：Proteomics

authors： Franco CF,Santos R,Coelho AV

更新日期：2011-09-01 00:00:00

abstract：:Cerebrotendinous xanthomatosis (CTX) is an autosomal recessive lipid-storage disorder caused by a deficiency of the mitochondrial sterol 27-hydroxylase. Genetic analysis utilizing SSCP and direct DNA sequencing identified a new mutation. One base-pair of cytosine was deleted at codon 326 on exon 2 of CYP27 in all CTX ...

journal_title：Proteomics

authors： Wang PW,Chang WN,Lu CH,Chao D,Schrag C,Pan TL

更新日期：2006-02-01 00:00:00

abstract：:Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...

journal_title：Proteomics

authors： Ahrné E,Müller M,Lisacek F

更新日期：2010-02-01 00:00:00

abstract：:An aim of proteome research is to identify the entire complement of proteins expressed in defined cell types of humans, animals, plants, and microorganisms. The approach requires searching for low abundant or even rarely expressed proteins in many cell types, as well as the determination of the protein expression leve...

journal_title：Proteomics

authors： Wildgruber R,Weber G,Wise P,Grimm D,Bauer J

更新日期：2014-03-01 00:00:00

abstract：:Human embryonic stem cells (hESCs) have received considerable attention due to their therapeutic potential and usefulness in understanding early development and cell fate commitment. In order to appreciate the unique properties of these pluripotent, self-renewing cells, we have performed an in-depth multidimensional f...

journal_title：Proteomics

authors： Zhao P,Schulz TC,Sherrer ES,Weatherly DB,Robins AJ,Wells L

更新日期：2015-01-01 00:00:00

abstract：:Cancer research is striving toward new frontiers of assigning the correct personalized drug(s) to a given patient. However, extensive tumor heterogeneity poses a major obstacle. Tumors of the same type often respond differently to therapy, due to patient-specific molecular aberrations and/or untargeted tumor subpopula...

journal_title：Proteomics

authors： Kravchenko-Balasha N

更新日期：2020-07-01 00:00:00

abstract：:During the peri-implantation period, molecular signaling between embryo and endometrium (layer of tissue lining the uterus lumen) is supposed to be crucial for the maintenance of pregnancy. To investigate embryo-induced alterations in the proteome of bovine endometrium in the preattachment period (day 18), we used mon...

journal_title：Proteomics

authors： Berendt FJ,Fröhlich T,Schmidt SE,Reichenbach HD,Wolf E,Arnold GJ

更新日期：2005-07-01 00:00:00

abstract：:Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected by Citrus exocortis viroid (CEVd). Among the differentially expressed proteins detected, 45 were su...

journal_title：Proteomics

authors： Lisón P,Tárraga S,López-Gresa P,Saurí A,Torres C,Campos L,Bellés JM,Conejero V,Rodrigo I

更新日期：2013-03-01 00:00:00

abstract：:Simpson-Golabi-Behmel syndrome (SGBS) is an X-linked condition shown to be the result of deletions of the glypican-3 (GPC3) gene. GPC3 is a proteoglycan localized to the cell membrane via a glycosylphosphatidyl-inositol (GPI) anchor. To further elucidate the GPC3 function(s), we have screened various cell lines for pr...

journal_title：Proteomics

authors： Davoodi J,Kelly J,Gendron NH,MacKenzie AE

更新日期：2007-06-01 00:00:00

abstract：:Head and neck cancers, including oral squamous cell carcinoma (OSCC), are the sixth most common malignancy in the world and are characterized by poor prognosis and a low survival rate. Saliva is oral fluid with intimate contact with OSCC. Besides non-invasive, simple, and rapid to collect, saliva is a potential source...

journal_title：Proteomics

authors： Kawahara R,Bollinger JG,Rivera C,Ribeiro AC,Brandão TB,Paes Leme AF,MacCoss MJ

更新日期：2016-01-01 00:00:00

abstract：:MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...

journal_title：Proteomics

authors： Helsens K,Martens L,Vandekerckhove J,Gevaert K

更新日期：2007-02-01 00:00:00

abstract：:Various types of unwanted and uncontrollable signal variations in MS-based metabolomics and proteomics datasets severely disturb the accuracies of metabolite and protein profiling. Therefore, pooled quality control (QC) samples are often employed in quality management processes, which are indispensable to the success ...

journal_title：Proteomics

authors： Wang S,Yang H

更新日期：2019-10-01 00:00:00

abstract：:Neurons present a highly polarized morphology, often displaying a significantly imbalanced distribution of the cytoplasm between the somatic and axonal domains. This imbalance requires cell-specific mechanisms for the maintenance of the axoplasmic mass during development, neuronal homeostasis, and recovery after injur...

journal_title：Proteomics

authors： Farias J,Sotelo JR,Sotelo-Silveira J

更新日期：2019-08-01 00:00:00

abstract：:The quantitative analysis of signaling networks requires highly sensitive methods for the time-resolved determination of protein phosphorylation. For this reason, we developed a quantitative protein microarray that monitors the activation of multiple signaling pathways in parallel, and at high temporal resolution. A l...

journal_title：Proteomics

authors： Korf U,Derdak S,Tresch A,Henjes F,Schumacher S,Schmidt C,Hahn B,Lehmann WD,Poustka A,Beissbarth T,Klingmüller U

更新日期：2008-11-01 00:00:00

abstract：:Gliadins and glutenins are the main protein fractions present in wheat gluten. They are responsible for technological and nutritional quality of wheat based products. In particular, glutenins are mainly responsible for dough visco-elastic properties, whereas gliadins confer extensibility to dough and are the most impo...

journal_title：Proteomics

authors： Ferrante P,Masci S,D'Ovidio R,Lafiandra D,Volpi C,Mattei B

更新日期：2006-03-01 00:00:00

abstract：:Off-line coupling of capillary IEF (CIEF) with matrix-assisted laser desorption/ionization mass spectrometry was utilized for the analysis of human blood serum. Serum proteins were initially separated by CIEF, and fractions of the isoelectric separation were eluted sequentially to a MALDI-TOF MS sample target. During ...

journal_title：Proteomics

authors： Crowley TA,Hayes MA

更新日期：2005-09-01 00:00:00

abstract：:Selected or multiple reaction monitoring is a targeted mass spectrometry method (S/MRM-MS), in which many peptides are simultaneously and consistently analyzed during a single liquid chromatography-mass spectrometry (LC-S/MRM-MS) measurement. These capabilities make S/MRM-MS an attractive method to monitor a consisten...

journal_title：Proteomics

authors： Ebhardt HA,Sabidó E,Hüttenhain R,Collins B,Aebersold R

更新日期：2012-04-01 00:00:00

abstract：:Protein arginine methyltransferase 5 (PRMT5) is a major enzyme responsible for generating monomethyl and symmetric dimethyl arginine in proteins. PRMT5 is essential for cell viability and development, and its overexpression is observed in a variety of cancers. In the present study, it is found that levels of PRMT5 pro...

journal_title：Proteomics

authors： Lim Y,Park YE,Ha S,Lee JE,Kim HC

更新日期：2020-05-28 00:00:00

abstract：:Sirtuins are a family of NAD(+)-dependent protein deacetylases that regulate cellular functions through deacetylation of a wide range of protein targets. Overexpression of Sir2, the first gene discovered in this family, is able to extend the life span in various organisms. The anti-aging effects of human homologues of...

journal_title：Proteomics

authors： Law IK,Liu L,Xu A,Lam KS,Vanhoutte PM,Che CM,Leung PT,Wang Y

更新日期：2009-05-01 00:00:00

abstract：:Mass spectrometry-based proteomics is used to gain insight into the abundance and subcellular localization of cellular signaling components, the composition of molecular complexes and the regulation of signaling pathways. Multicellular organisms have evolved signaling networks and fast responses to stimuli that can be...

journal_title：Proteomics

authors： Kaufmann K,Smaczniak C,de Vries S,Angenent GC,Karlova R

更新日期：2011-02-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry (MS/MS) were used to determine the multiple acetylation sites in the histone acetyltransferase (HAT): p300-HAT. Partial cleavage of the peptides containing acetylated lysine residues by trypsin provided a set of nested sequences...

journal_title：Proteomics

authors： Wang D,Thompson P,Cole PA,Cotter RJ

更新日期：2005-06-01 00:00:00

abstract：:Diurnal physiological acclimation regulated by a circadian system is an advantage for plant fitness. The circadian system is composed of a signal input, the clock and output pathways. Understanding the regulation mechanism of the output pathways remains a major challenge. Diurnal proteomic change reflects the state of...

journal_title：Proteomics

authors： Wang Z,Wang T

更新日期：2011-01-01 00:00:00

abstract：:Prostate cancer is one of the leading cancers in men. Taking dietary supplements, such as fish oil (FO), which is rich in n-3 polyunsaturated fatty acids (PUFAs), has been employed as a strategy to lower prostate cancer risk and control disease progression. In this study, we investigated the global phosphoproteomic ch...

journal_title：Proteomics

authors： Zhao H,Pflug BR,Lai X,Wang M

更新日期：2016-09-01 00:00:00

abstract：:High-density lipoprotein (HDL) is the most abundant lipoprotein particle in the plasma and a negative risk factor of atherosclerosis. By using a proteomic approach it is possible to obtain detailed information about its protein content and protein modifications that may give new information about the physiological rol...

journal_title：Proteomics

authors： Karlsson H,Leanderson P,Tagesson C,Lindahl M

更新日期：2005-04-01 00:00:00

abstract：:The aims of the study were to: (i) identify differentially regulated proteins in cerebrospinal fluid (CSF) between multiple sclerosis (MS) patients and non-MS controls; (ii) examine the effect of matching the CSF samples on either total protein amount or volume, and compare four protein normalization strategies for CS...

journal_title：Proteomics

authors： Opsahl JA,Vaudel M,Guldbrandsen A,Aasebø E,Van Pesch V,Franciotta D,Myhr KM,Barsnes H,Berle M,Torkildsen Ø,Kroksveen AC,Berven FS

更新日期：2016-04-01 00:00:00

abstract：:A novel strain of influenza A H1N1 emerged in the spring of 2009 and has spread rapidly throughout the world. Although vaccines have recently been developed that are expected to be protective, their availability was delayed until well into the influenza season. Although anti-influenza drugs such as neuraminidase inhib...

journal_title：Proteomics

authors： Chen CH,Zhang XQ,Lo CW,Liu PF,Liu YT,Gallo RL,Hsieh MF,Schooley RT,Huang CM

更新日期：2010-06-01 00:00:00