Abstract:
:High-density lipoprotein (HDL) is the most abundant lipoprotein particle in the plasma and a negative risk factor of atherosclerosis. By using a proteomic approach it is possible to obtain detailed information about its protein content and protein modifications that may give new information about the physiological roles of HDL. In this study the two subfractions; HDL(2) and HDL(3), were isolated by two-step discontinuous density-gradient ultracentrifugation and the proteins were separated with two-dimensional gel electrophoresis and identified with peptide mass fingerprinting, using matrix-assisted laser desorption/ionisation time of flight mass spectrometry. Identified proteins in HDL were: the dominating apo A-I as six isoforms, four of them with a glycosylation pattern and one of them with retained propeptide, apolipoprotein (apo) A-II, apo A-IV, apo C-I, apo C-II, apo C-III (two isoforms), apo E (five isoforms), the recently discovered apo M (two isoforms), serum amyloid A (two isoforms) and serum amyloid A-IV (six isoforms). Furthermore, alpha-1-antitrypsin was identified in HDL for the first time. Additionally, salivary alpha-amylase was identified as two isoforms in HDL(2), and apo L and a glycosylated apo A-II were identified in HDL(3). Besides confirming the presence of different apolipoproteins, this study indicates new patterns of glycosylated apo A-I and apo A-II. Furthermore, the study reveals new proteins in HDL; alpha-1-antitrypsin and salivary alpha-amylase. Further investigations about these proteins may give new insight into the functional role of HDL in coronary artery diseases.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Karlsson H,Leanderson P,Tagesson C,Lindahl Mdoi
10.1002/pmic.200401010subject
Has Abstractpub_date
2005-04-01 00:00:00pages
1431-45issue
5eissn
1615-9853issn
1615-9861journal_volume
5pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse live...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000126
更新日期:2010-11-01 00:00:00
abstract::Staphylococcus aureus Clp ATPases (molecular chaperones) alter normal physiological functions including an aconitase-mediated effect on post-stationary growth, acetate catabolism, and entry into death phase (Chatterjee et al., J. Bacteriol. 2005, 187, 4488-4496). In the present study, the global function of ClpC in ph...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800586
更新日期:2009-03-01 00:00:00
abstract::We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chrom...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500511
更新日期:2006-03-01 00:00:00
abstract::Phage display techniques using random peptide interactions have supported the role of mammalian glutathione transferase (GST) as part of a signalling pathway for both oxidative stress and an apoptosis pathway. Little is known about the interaction of nonmammalian GST with other proteins. GSTs have been implicated in t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300719
更新日期:2004-07-01 00:00:00
abstract::Protein expression changes induced in thioglycolate-elicited peritoneal murine macrophages (M Phi) by infection with type III Group B Streptococcus (GBS) are described. Proteins from control M Phi and M Phi incubated 2 h with live or heat-inactivated GBS were separated by 2-DE. Proteins whose expression was significan...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900642
更新日期:2010-06-01 00:00:00
abstract::Caulobacter crescentus, a Gram negative alpha-purple bacterium that displays an invariant asymmetric cell division pattern, has become a key model system for the study of bacterial development. Membrane proteins play key roles in cell cycle events, both as components of landmark morphological structures and as critica...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200104)1:5<705::AID-PROT705>3.0.
更新日期:2001-05-01 00:00:00
abstract::Seed coat color is an important attribute determining consumption of soybean seeds. Soybean cultivar Mallikong (M) has yellow seed coat while its naturally mutated cultivar Mallikong mutant (MM), has brown colored seed coat. We used integrated proteomics and metabolomics approach to investigate the differences between...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400453
更新日期:2015-05-01 00:00:00
abstract::Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600373
更新日期:2006-12-01 00:00:00
abstract::The Annual 2014 Spring Workshop of the Proteomics Standards Initiative (PSI) of the Human Proteome Organization (HUPO) was held this year jointly with the metabolomics COordination of Standards in MetabOlomicS (COSMOS) group. The range of existing MS standards (mzML, mzIdentML, mzQuantML, mzTab, TraML) was reviewed an...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.201470164
更新日期:2014-11-01 00:00:00
abstract::The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was dev...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500001
更新日期:2005-11-01 00:00:00
abstract::More than 300 different types of protein post-translational modifications (PTMs) have been described, many of which are known to have pivotal roles in cellular physiology and disease. Nevertheless, only a handful of PTMs have been extensively investigated at the proteome level. Knowledge of protein substrates and thei...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200900398
更新日期:2009-10-01 00:00:00
abstract::Campylobacter jejuni is a major gastrointestinal pathogen that colonizes host mucosa via interactions with extracellular matrix proteins, such as fibronectin (Fn). Fn-binding is mediated by a 37 kDa outer membrane protein termed Campylobacter adherence Factor (CadF). The outer membrane protein profile of a recent gast...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900440
更新日期:2010-01-01 00:00:00
abstract::We have developed a proteome database (DB), BiomarkerDigger (http://biomarkerdigger.org) that automates data analysis, searching, and metadata-gathering function. The metadata-gathering function searches proteome DBs for protein-protein interaction, Gene Ontology, protein domain, Online Mendelian Inheritance in Man, a...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800593
更新日期:2009-07-01 00:00:00
abstract::The quantitative analysis of signaling networks requires highly sensitive methods for the time-resolved determination of protein phosphorylation. For this reason, we developed a quantitative protein microarray that monitors the activation of multiple signaling pathways in parallel, and at high temporal resolution. A l...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800112
更新日期:2008-11-01 00:00:00
abstract::Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000745
更新日期:2011-09-01 00:00:00
abstract::A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500721
更新日期:2006-05-01 00:00:00
abstract::The ZNF198-fibroblast growth factor receptor-1 (FGFR1) fusion kinase is a constitutively activated tyrosine kinase associated with a specific atypical myeloproliferative disease. The chimeric protein localizes to the cytoplasm, unlike the wild type FGFR1 receptor kinase, and presumably inappropriately phosphorylates s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800852
更新日期:2009-08-01 00:00:00
abstract::Green tea polyphenols exhibit multiple antitumor activities, and the mechanisms of action are not completely understood. Previously, we reported that green tea extract (GTE)-induced actin remolding is associated with increased cell adhesion and decreased motility in A549 lung cancer cells. To identify the cellular tar...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800019
更新日期:2009-02-01 00:00:00
abstract::Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% of CW mass) and proteins (5-10%) that play major roles in CW plasticity during development and in response to environmental cues. Here, we present CW proteomics data of Arabidopsis thaliana roots. Plants were cultivated in hydroponic conditio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500129
更新日期:2016-02-01 00:00:00
abstract::Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800009
更新日期:2008-07-01 00:00:00
abstract::MS/MS has been used to improve genome annotation in various organisms. The classical approach is to construct comprehensive theoretical peptide database with six frame translation model from the whole ORF of a genome and search against this database with real MS/MS spectra. In this work we took a more focused approach...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400174
更新日期:2014-12-01 00:00:00
abstract::In this work, for the first time, perfluorinated magnetic mesoporous microspheres were designed and synthesized for the highly specific enrichment of fluorous-derivatized phosphopeptides through the unique fluorine-fluorine interactions. The perfluorinated magnetic mesoporous microspheres were prepared through a surfa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500323
更新日期:2016-04-01 00:00:00
abstract::Protein arginine methylation is a PTM catalyzed by an evolutionarily conserved family of enzymes called protein arginine methyltransferases (PRMTs), with PRMT1 being the most conserved member of this enzyme family. This modification has emerged to be an important regulator of protein functions. To better understand th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200132
更新日期:2012-11-01 00:00:00
abstract::In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400785
更新日期:2004-09-01 00:00:00
abstract::Identification of mucin-type O-glycosylated proteins with known functions in model organisms like Drosophila could provide keys to elucidate functions of the O-glycan moiety and proteomic analyses of O-glycoproteins in higher eukaryotes remain a challenge due to structural heterogeneity and a lack of efficient tools f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600793
更新日期:2007-09-01 00:00:00
abstract::FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200210)2:10<1435::AID-PROT1435>3
更新日期:2002-10-01 00:00:00
abstract::SELDI-TOF-MS is rapidly gaining popularity as a screening tool for clinical applications of proteomics. Application of adequate statistical techniques in all the stages from measurement to information is obligatory. One of the statistical methods often used in proteomics is classification: the assignment of subjects t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700046
更新日期:2007-10-01 00:00:00
abstract::Tectona grandis L. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree select...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100183
更新日期:2012-04-01 00:00:00
abstract::The Japanese red and pink corals are known to be precious because of their commercial value resulting from their use in ornaments, jewelry, and medicine. Precious corals are very interesting models for biomineralization studies and possess two different skeletal structures: an axial skeleton and an endoskeleton (scler...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300519
更新日期:2014-11-01 00:00:00
abstract::In this study, a method, based on dual column affinity chromatography hyphenated to isotope dilution inductively coupled plasma-quadrupole MS, was developed for selenium determination in selenoprotein P, glutathione peroxidase, and selenoalbumin in human serum samples from a group of healthy volunteers (n=399). Method...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900677
更新日期:2010-10-01 00:00:00