Phosphorylation of the SSBP2 and ABL proteins by the ZNF198-FGFR1 fusion kinase seen in atypical myeloproliferative disorders as revealed by phosphopeptide-specific MS.

abstract：:The comprehension of sexually different responses in dioecious plants to excess manganese (Mn) stress requires molecular explanation. Physiological and proteomic changes in leaves of Populus cathayana males and females were analyzed after 4 wk of exposure to Mn stress. Under excess Mn conditions, shoot height and phot...

journal_title：Proteomics

authors： Chen F,Zhang S,Zhu G,Korpelainen H,Li C

更新日期：2013-08-01 00:00:00

abstract：:The quality of samples and of pre-analytical steps are crucial in all biological tests, this is dramatically true in proteomics analysis. In renal cell carcinoma preparation for two-dimensional gel electrophoresis the time elapsed between sample collection and treatment, and the heterogeneity of tissues are considered...

journal_title：Proteomics

authors： Sarto C,Valsecchi C,Mocarelli P

更新日期：2002-11-01 00:00:00

abstract：:Exercise training has been recommended as a nonpharmacological strategy for the prevention and attenuation of skeletal muscle atrophy in distinct pathophysiological conditions. Despite the well-established phenotypic alterations, the molecular mechanisms underlying exercise-induced skeletal muscle remodeling are poorl...

journal_title：Proteomics

authors： Padrão AI,Ferreira R,Amado F,Vitorino R,Duarte JA

更新日期：2016-03-01 00:00:00

abstract：:In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...

journal_title：Proteomics

authors： Juan HF,Chen JH,Hsu WT,Huang SC,Chen ST,Yi-Chung Lin J,Chang YW,Chiang CY,Wen LL,Chan DC,Liu YC,Chen YJ

更新日期：2004-09-01 00:00:00

abstract：:Extracellular vesicles (EVs) are lipid-bilayered vesicles that are released by multiple cell types and contain nucleic acids and proteins. Very little is known about how the cargo is packaged into EVs. Ubiquitination of proteins is a key posttranslational modification that regulates protein stability and trafficking t...

journal_title：Proteomics

authors： Anand S,Foot N,Ang CS,Gembus KM,Keerthikumar S,Adda CG,Mathivanan S,Kumar S

更新日期：2018-09-01 00:00:00

abstract：:CID has become a routine method for fragmentation of peptides in shotgun proteomics, whereas electron transfer dissociation (ETD) has been described as a preferred method for peptides carrying labile PTMs. Though both of these fragmentation techniques have their obvious advantages, they also have their own drawbacks. ...

journal_title：Proteomics

authors： Kim MS,Zhong J,Kandasamy K,Delanghe B,Pandey A

更新日期：2011-06-01 00:00:00

abstract：:With advances in liquid chromatography coupled to tandem mass spectrometry technologies combined with the continued goals of biomarker discovery, clinical applications of established biomarkers, and integrating large multiomic datasets (i.e. "big data"), there remains an urgent need for robust tools to assess instrume...

journal_title：Proteomics

authors： Bereman MS

更新日期：2015-03-01 00:00:00

abstract：:The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better unders...

journal_title：Proteomics

authors： Victor B,Kanobana K,Gabriël S,Polman K,Deckers N,Dorny P,Deelder AM,Palmblad M

更新日期：2012-06-01 00:00:00

abstract：:The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...

journal_title：Proteomics

authors： Marengo E,Robotti E,Antonucci F,Cecconi D,Campostrini N,Righetti PG

更新日期：2005-02-01 00:00:00

abstract：:We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chrom...

journal_title：Proteomics

authors： Baek MC,Kim SJ,Yea K,Kim Y,Lee BD,Kim J,Lee HJ,Kang MH,Choi SK,Kim JI,Lee TG,Suh PG,Ryu SH

更新日期：2006-03-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a major complication of chronic viral hepatitis C. Therapy for HCC is still disappointing. It is thus of great importance to identify novel HCC markers for early detection of the disease, and tumor-specific proteins as potential therapeutic targets. We have used a proteomic approach t...

journal_title：Proteomics

authors： Blanc JF,Lalanne C,Plomion C,Schmitter JM,Bathany K,Gion JM,Bioulac-Sage P,Balabaud C,Bonneu M,Rosenbaum J

更新日期：2005-09-01 00:00:00

abstract：:Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...

journal_title：Proteomics

authors： Ahsan N,Renaut J,Komatsu S

更新日期：2009-05-01 00:00:00

abstract：:Roots, leaves, and intermediate sections between roots and leaves (ISRL) of wheat seedlings show different physiological functions at the protein level. We performed the first integrative proteomic analysis of different tissues of the drought-tolerant wheat cultivar Hanxuan 10 (HX-10) and drought-sensitive cultivar Ch...

journal_title：Proteomics

authors： Hao P,Zhu J,Gu A,Lv D,Ge P,Chen G,Li X,Yan Y

更新日期：2015-05-01 00:00:00

abstract：:Uni- or multidimensional microcapillary liquid chromatography (microLC) matrix-assisted laser desorption/ionization (MALDI) tandem mass spectrometry (MS/MS) approaches have gained significant attention for quantifying and identifying proteins in complex biological samples. The off-line coupling of microLC with MS quan...

journal_title：Proteomics

authors： Graber A,Juhasz PS,Khainovski N,Parker KC,Patterson DH,Martin SA

更新日期：2004-02-01 00:00:00

abstract：:Over the last decade, an increasing prevalence of peanut allergies was observed worldwide. Peanuts are meanwhile categorized among the most dangerous food allergens. This is particularly relevant since peanut-derived ingredients are widely used in industrial food production. To minimize the problem of hidden food alle...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Latendorf T,Nebendahl M,Petersen A,Krause S,Leippe M,Becker WM,Janssen O

更新日期：2009-07-01 00:00:00

abstract：:An improved method for sample preparation for MALDI-MS and MS/MS using AnchorChip targets is presented. The method, termed the SMW method (sample, matrix wash), results in better sensitivity for peptide mass fingerprinting as well as for sequencing by MS/MS than previously published methods. The method allows up-conce...

journal_title：Proteomics

authors： Zhang X,Shi L,Shu S,Wang Y,Zhao K,Xu N,Liu S,Roepstorff P

更新日期：2007-07-01 00:00:00

abstract：:High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to...

journal_title：Proteomics

authors： Majoul T,Bancel E,Triboï E,Ben Hamida J,Branlard G

更新日期：2003-02-01 00:00:00

abstract：:The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...

journal_title：Proteomics

authors： Ortsäter H,Sundsten T,Lin JM,Bergsten P

更新日期：2007-09-01 00:00:00

abstract：:Biology is increasingly dependent on large-scale analysis, such as proteomics, creating a requirement for efficient bioinformatics. Bioinformatic predictions of biological functions rely upon correctly annotated database sequences, and the presence of inaccurately annotated or otherwise poorly described sequences intr...

journal_title：Proteomics

authors： Bengtsson-Palme J,Boulund F,Edström R,Feizi A,Johnning A,Jonsson VA,Karlsson FH,Pal C,Pereira MB,Rehammar A,Sanchez J,Sanli K,Thorell K

更新日期：2016-09-01 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis has long been recognized as the causative agent of Johne's disease, a chronic inflammatory intestinal disease of sheep, cattle and other ruminants. Mycobacterial cells are extremely hardy, and proteomic analyses require the use of harsh conditions to effect their disruption....

journal_title：Proteomics

authors： Lanigan MD,Vaughan JA,Shiell BJ,Beddome GJ,Michalski WP

更新日期：2004-04-01 00:00:00

abstract：:Human embryonic stem cells (hESCs) have received considerable attention due to their therapeutic potential and usefulness in understanding early development and cell fate commitment. In order to appreciate the unique properties of these pluripotent, self-renewing cells, we have performed an in-depth multidimensional f...

journal_title：Proteomics

authors： Zhao P,Schulz TC,Sherrer ES,Weatherly DB,Robins AJ,Wells L

更新日期：2015-01-01 00:00:00

abstract：:A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...

journal_title：Proteomics

authors： Liu X,Gygi SP,Paulo JA

更新日期：2021-01-01 00:00:00

abstract：:"Liquid biopsies" have received attention as a complementary tool for traditional tissue biopsies that may enhance the spectrum of analysis for tumor-derived factors. One such factor gaining prominence in the liquid biopsy field is extracellular vesicles (EVs), membrane-bound nanovesicles which are secreted by cells i...

journal_title：Proteomics

authors： Ruhen O,Meehan K

更新日期：2019-01-01 00:00:00

abstract：:A simple and rapid method for characterizing hydrophobic integral membrane proteins and its utility for membrane proteomics using microcapillary liquid chromatography coupled on-line with tandem mass spectrometry (microLC-MS/MS) is described. The present technique does not rely on the use of detergents, strong organic...

journal_title：Proteomics

authors： Blonder J,Conrads TP,Yu LR,Terunuma A,Janini GM,Issaq HJ,Vogel JC,Veenstra TD

更新日期：2004-01-01 00:00:00

abstract：:To investigate the dynamic cellular response to a condition change, selective labeling of the nascent proteome is necessary. Here, we report a method combining click chemistry protein labeling with 2D DIGE. To test the relevance of the method, we compared nascent proteomes of actively growing bacterial cells with that...

journal_title：Proteomics

authors： Osterman IA,Ustinov AV,Evdokimov DV,Korshun VA,Sergiev PV,Serebryakova MV,Demina IA,Galyamina MA,Govorun VM,Dontsova OA

更新日期：2013-01-01 00:00:00

abstract：:Proteomics is increasingly being used to understand enzyme expression and regulatory mechanisms involved in the accumulation of storage reserves in crops with sequenced genomes. During the past six years, considerable progress has been made to characterize proteomes of both mature and developing seeds, particularly oi...

journal_title：Proteomics

authors： Hajduch M,Matusova R,Houston NL,Thelen JJ

更新日期：2011-05-01 00:00:00

abstract：:The proteomic profile of hypothalamus, a key organ of CNS, is explored here by employing two widely used MS techniques, i.e. HPLC/ESI-ion trap and HPLC/ESI-quadrupole-TOF MS. Strong cation exchange is used for the fractionation of peptides and protein search engine MASCOT is employed for data query. One hundred and th...

journal_title：Proteomics

authors： Iqbal J,Li W,Ullah K,Hasan M,Linna G,Awan U,Zhang Y,Batool S,Qing H,Deng Y

更新日期：2013-08-01 00:00:00

abstract：:Proteolytic modification of components of the extracellular milieu by metalloproteinases plays important roles in the regulation of multiple cellular and physiological processes and pathological conditions. ADAMTS1 is a secreted enzyme of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) fami...

journal_title：Proteomics

authors： Canals F,Colomé N,Ferrer C,Plaza-Calonge Mdel C,Rodríguez-Manzaneque JC

更新日期：2006-04-01 00:00:00

abstract：:Recent advances in two-dimensional electrophoresis (2-DE) such as fluorescent 2-D differential gel electrophoresis (2-D DIGE) has made it possible to detect and quantitate the critical changes involved in disease pathogenesis. We have previously identified novel proteins with altered expression in primary colorectal c...

journal_title：Proteomics

authors： Nishimori T,Tomonaga T,Matsushita K,Oh-Ishi M,Kodera Y,Maeda T,Nomura F,Matsubara H,Shimada H,Ochiai T

更新日期：2006-02-01 00:00:00

abstract：:The proteins recovered in exhaled breath condensate (EBC) might be used to non-invasively monitor respiratory diseases. However, the range of proteins and their source are still unresolved and contamination by saliva or a similar protein pattern in the nasal and bronchial compartments may make interpretation of the da...

journal_title：Proteomics

authors： Griese M,Noss J,von Bredow C

更新日期：2002-06-01 00:00:00