Abstract:
:Mycobacterium avium subsp. paratuberculosis has long been recognized as the causative agent of Johne's disease, a chronic inflammatory intestinal disease of sheep, cattle and other ruminants. Mycobacterial cells are extremely hardy, and proteomic analyses require the use of harsh conditions to effect their disruption. We compared the effectiveness of bead beating and sonication as cell lysis methods for the extraction of the proteomes of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis. Broad and narrow range two-dimensional gel electrophoresis was used to compare the numbers of silver stained protein spots that were observed in mycobacterial lysates. Despite differences in the yield of total protein from either species, and at different ages, the two methods appeared to give similar representations of the mycobacterial proteomes analyzed. Bead beating therefore represents a rapid and effective method of extracting the proteomes of mycobacterial species without the risks associated with an open tube sonication procedure.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Lanigan MD,Vaughan JA,Shiell BJ,Beddome GJ,Michalski WPdoi
10.1002/pmic.200300672subject
Has Abstractpub_date
2004-04-01 00:00:00pages
1094-100issue
4eissn
1615-9853issn
1615-9861journal_volume
4pub_type
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