Abstract:
:After hind limb suspension, a remodeling of postural muscle phenotype is observed. This remodeling results in a shift of muscle profile from slow-oxidative to fast-glycolytic. These metabolic changes and fiber type shift increase muscle fatigability. Acetyl-L-carnitine (ALCAR) influences the skeletal muscle phenotype of soleus muscle suggesting a positive role of dietary supplementation of ALCAR during unloading. In the present study, we applied a 2-D DIGE, mass spectrometry and biochemical assays, to assess qualitative and quantitative differences in the proteome of rat slow-twitch soleus muscle subjected to disuse. Meanwhile, the effects of ALCAR administration on muscle proteomic profile in both unloading and normal-loading conditions were evaluated. The results indicate a modulation of troponin I and tropomyosin complex to regulate fiber type transition. Associated, or induced, metabolic changes with an increment of glycolytic enzymes and a decreased capacity of fat oxidation are observed. These metabolic changes appear to be counteracted by ALCAR treatment, which restores the mitochondrial mass and decreases the glycolytic enzyme expression, suggesting a normalization of the metabolic shift observed in unloaded animals. This normalization is accompanied by a maintenance of body weight and seems to prevent a switch of fiber type.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Moriggi M,Cassano P,Vasso M,Capitanio D,Fania C,Musicco C,Pesce V,Gadaleta MN,Gelfi Cdoi
10.1002/pmic.200701176subject
Has Abstractpub_date
2008-09-01 00:00:00pages
3588-604issue
17eissn
1615-9853issn
1615-9861journal_volume
8pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic F. oxysporum strains. In this study, F. oxysporum wild-type (WT) MSA 35, a biocontrol multi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900716
更新日期:2010-09-01 00:00:00
abstract::The array of biomolecules generated by a functioning ecosystem represents both a potential resource for sustainable harvest and a potential indicator of ecosystem health and function. The cupped leaves of the carnivorous pitcher plant, Sarracenia purpurea, harbor a dynamic food web of aquatic invertebrates in a fully ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000256
更新日期:2011-06-01 00:00:00
abstract::Myostatin plays a major role in muscle growth and development and animals with disruption of this gene display marked increases in muscle mass. Little is known about muscle physiological adaptations in relation to this muscle hypertrophy. To provide a more comprehensive view, we analyzed bovine muscles from control, h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400925
更新日期:2005-02-01 00:00:00
abstract::We present here the first proteomics analysis of uveal melanoma (UM) cells. These cells represent a good model for the identification of polypeptide markers, which could be developed as diagnostic tools. UM is the most common primary intraocular tumour in adults. In contrast to other cancers, the survival rate of pati...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500030
更新日期:2005-12-01 00:00:00
abstract::Hpn is a small histidine-rich cytoplasmic protein from Helicobacter pylori and has been recognized as a high-risk factor for several cancers including gastric cancer, colorectal cancer, and MALT lymphoma. However, the relationship between Hpn and cancers remains elusive. In this study, we discovered that Hpn protein e...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600350
更新日期:2017-06-01 00:00:00
abstract::Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high-throughput ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300353
更新日期:2014-02-01 00:00:00
abstract::MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200501332
更新日期:2006-01-01 00:00:00
abstract::We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HP...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500635
更新日期:2006-04-01 00:00:00
abstract::Recently, we introduced a novel proteomics method employing a metalloendopeptidase with Lys-N specificity to produce proteolytic peptides. Fragmentation spectra generated by electron transfer dissociation, for a large proportion of the Lys-N proteolytic peptides, were found to be dominated by extensive series of c-typ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900405
更新日期:2010-03-01 00:00:00
abstract::Antibiotics disturb the physiological homeostasis of bacterial cells by interfering with essential cellular functions or structures. The bacterial proteome adjusts quickly in response to antibiotic challenge. This physiological response is specifically tailored to overcome the inflicted damage and, thus, closely linke...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100046
更新日期:2011-08-01 00:00:00
abstract::To better understand the mechanism underlying hepatocellular carcinoma (HCC) metastasis and to search for potential markers for HCC prognosis, differential proteome analysis on two HCC cell strains with high and low metastatic potentials, MHCC97-H and MHCC97-L, was conducted using two-dimensional (2-D) gel electrophor...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300653
更新日期:2004-04-01 00:00:00
abstract::Site-specific isomerization of uridines into pseudouridines in RNAs is catalyzed either by stand-alone enzymes or by box H/ACA ribonucleoprotein particles (sno/sRNPs). The archaeal box H/ACA sRNPs are five-component complexes that consist of a guide RNA and the aCBF5, aNOP10, L7Ae, and aGAR1 proteins. In this study, w...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400529
更新日期:2015-08-01 00:00:00
abstract::Human galectin-3 (hGal-3) is a mammalian lectin involved in regulation of RNA splicing, apoptosis, cell differentiation, and proliferation. Multimerized extracellular hGal-3 is thought to crosslink cells by binding to glycoproteins and glycosylated cancer antigens on the cell surface or extracellular matrix. Fluoresce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900581
更新日期:2010-05-01 00:00:00
abstract::Voltage-dependent anion channel (VDAC) proteins are small, abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apopt...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300734
更新日期:2004-05-01 00:00:00
abstract::The existence of a gamma-glutamyl cycle consisting of intracellular GSH synthesis, extrusion to the apoplastic space and recovery by gamma-glutamyl transferase (GGT)-assisted degradation into its constituent amino acids, has been demonstrated in plants. To address the significance of this cycle in plant cells, we perf...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200479
更新日期:2013-06-01 00:00:00
abstract::Panax ginseng is an important herb that has clear effects on the treatment of diverse diseases. Until now, the natural peptide constitution of this herb remains unclear. Here, we conduct an extensive characterization of Ginseng peptidome using MS-based data mining and sequencing. The screen on the charge states of pre...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600183
更新日期:2016-11-01 00:00:00
abstract::A method for quantitative proteomic analysis based on the selective isolation of multiply charged peptides (RH peptides) containing arginine and histidine residues is described. Two pools of proteins are digested in tandem with lysyl-endopeptidase and trypsin and the primary amino groups of proteolytic peptides are se...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500836
更新日期:2006-08-01 00:00:00
abstract::MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600682
更新日期:2007-02-01 00:00:00
abstract::Rhodococcus sp. strain TFB is a versatile gram-positive bacterium able to grow on a wide variety of aromatic compounds as carbon and energy sources. Since the strain is refractory to genetic analysis, a proteomic approach was used to study the metabolic pathways involved in the catabolism of such compounds by analyzin...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500422
更新日期:2006-04-01 00:00:00
abstract::The senescence-accelerated mouse (SAM) is a useful animal model to study aging or age-associated disorders due to its inherited aging phenotype. To investigate proteins involved in the aging process in liver, we compared the young (4- or 20-week old) and the aged group (50-week-old) of SAMP8 (short life span) and SAMR...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300562
更新日期:2003-10-01 00:00:00
abstract::This study describes the gel-free phosphoproteomic analysis of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea grown in vitro under nonlimiting conditions. Using a combination of strong cation exchange and IMAC prior to LC-MS, we identified over 1350 phosphopeptides per fungus representing over ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300541
更新日期:2014-07-01 00:00:00
abstract::Immobilized metal affinity chromatography (IMAC) is a commonly used technique for phosphoproteome analysis due to its high affinity for adsorption of phosphopeptides. Miniaturization of IMAC column is essential for the analysis of a small amount of sample. Nanoscale IMAC column was prepared by chemical modification of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600045
更新日期:2007-02-01 00:00:00
abstract::The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900109
更新日期:2019-11-01 00:00:00
abstract::Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A4...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400835
更新日期:2004-10-01 00:00:00
abstract::By mining publicly accessible databases, we have developed a collection of tissue-specific predictive protein expression maps as a function of cancer histological state. Data analysis is applied to the differential expression of gene products in pooled libraries from the normal to the altered state(s). We wish to repo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300488
更新日期:2003-08-01 00:00:00
abstract::Ubiquitin-dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two-hybrid analysis of their interactions with each other. We observed that there are 114 interacting-pairs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700588
更新日期:2008-02-01 00:00:00
abstract::A diverse range of proteins are modified by the post-translational covalent attachment of a glycosyl-phosphatidylinositol (GPI) membrane anchor. Hydropathy plots and other computer algorithms can be used to predict the presence of a GPI anchor attachment signal in the nascent polypeptide chain. However, the presence o...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/1615-9861(200106)1:6<748::AID-PROT748>3.0.
更新日期:2001-06-01 00:00:00
abstract::Myelination of the CNS is performed by oligodendrocytes (OLs), which have been implicated in brain disorders, such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an OL reference proteome database. Proteins were prefractionationated by SDS-PAGE and after i...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300201
更新日期:2013-12-01 00:00:00
abstract::The large interactive surfaces of nanoparticles (NPs) increase the opportunities to develop NPs for vascular targeting. Proteomic analysis of endothelial cells exposed to NPs reveals the cellular response and turns the focus into the impairment of the endothelial permeability. Here, quantitative proteomics and transcr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800228
更新日期:2019-03-01 00:00:00
abstract::Clostridium cellulolyticum is a model mesophilic anaerobic bacterium that efficiently degrades plant cell walls. The recent genome release offers the opportunity to analyse its complete degradation system. A total of 148 putative carbohydrate-active enzymes were identified, and their modular structures and activities ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900311
更新日期:2010-02-01 00:00:00