Abstract:
:Panax ginseng is an important herb that has clear effects on the treatment of diverse diseases. Until now, the natural peptide constitution of this herb remains unclear. Here, we conduct an extensive characterization of Ginseng peptidome using MS-based data mining and sequencing. The screen on the charge states of precursor ions indicated that Ginseng is a peptide-rich herb in comparison of a number of commonly used herbs. The Ginseng peptides were then extracted and submitted to nano-LC-MS/MS analysis using different fragmentation modes, including CID, high-energy collisional dissociation, and electron transfer dissociation. Further database search and de novo sequencing allowed the identification of total 308 peptides, some of which might have important biological activities. This study illustrates the abundance and sequences of endogenous Ginseng peptides, thus providing the information of more candidates for the screening of active compounds for future biological research and drug discovery studies.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Ye X,Zhao N,Yu X,Han X,Gao H,Zhang Xdoi
10.1002/pmic.201600183subject
Has Abstractpub_date
2016-11-01 00:00:00pages
2788-2791issue
21eissn
1615-9853issn
1615-9861journal_volume
16pub_type
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