Abstract:
:Identification and characterization of biomarkers in body fluids such as serum or urine serve as a basis for early detection of diseases, particularly of cancer. Performing 2-DE with subsequent MS analyses, conventional immunoblotting and immunohistochemistry we identified two proteins, orosomucoid (ORM) and human zinc-alpha(2)-glycoprotein (ZAG), which were increased in the urine samples of patients with bladder cancer in comparison to the urine samples of healthy volunteers. The highest amount of both proteins was found in invasive bladder cancer stages such as pT2-3. Immunohistochemical studies showed ORM in inflammatory cells but also in endothelial cells of blood vessels within or adjacent to the tumor area and in part of the tumor cells. ZAG was prominent in tumor cells at the tumor invasion front. Additionally, ZAG was localized at the luminal surface of normal urothelium, which switches to the basal side when a superficial papillary tumor was observed. These results show that we have been able to identify two new proteins that may be related to the development of superficial bladder cancer and to its switch to an invasive phenotype.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Irmak S,Tilki D,Heukeshoven J,Oliveira-Ferrer L,Friedrich M,Huland H,Ergün Sdoi
10.1002/pmic.200402005subject
Has Abstractpub_date
2005-11-01 00:00:00pages
4296-304issue
16eissn
1615-9853issn
1615-9861journal_volume
5pub_type
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