Abstract:
:Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient sensitivity for, e.g. detecting low-abundance biomarkers likely present at the low ng/mL to pg/mL range in human blood plasma or serum, or extremely low-abundance signaling proteins in cells or tissues. Herein, we review recent advances in methods and technologies, including front-end immunoaffinity depletion, fractionation, selective enrichment of target proteins/peptides including posttranslational modifications, as well as advances in MS instrumentation which have significantly enhanced the overall sensitivity of SRM assays and enabled the detection of low-abundance proteins at low- to sub-ng/mL level in human blood plasma or serum. General perspectives on the potential of achieving sufficient sensitivity for detection of pg/mL level proteins in plasma are also discussed.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Shi T,Su D,Liu T,Tang K,Camp DG 2nd,Qian WJ,Smith RDdoi
10.1002/pmic.201100436subject
Has Abstractpub_date
2012-04-01 00:00:00pages
1074-92issue
8eissn
1615-9853issn
1615-9861journal_volume
12pub_type
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