Abstract:
:Sequencing of the human genome opened the way to the exploration of the proteome and this has lead to the identification of large numbers of proteins in complex biological samples. The identification of diagnostic patterns in samples taken from patients to aid diagnosis is in the early stages of development. The solution to many of the technical challenges in proteomics and protein based molecular diagnostics will be found in new applications of nanomaterials. This review describes some of the physical and chemical principles underlying nanomaterials and devices and outlines how they can be used in proteomics; developments which are establishing nanoproteomics as a new field. Nanoproteomics will provide the platform for the discovery of next generation biomarkers. The field of molecular diagnostics will then come of age.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Johnson CJ,Zhukovsky N,Cass AE,Nagy JMdoi
10.1002/pmic.200700665subject
Has Abstractpub_date
2008-02-01 00:00:00pages
715-30issue
4eissn
1615-9853issn
1615-9861journal_volume
8pub_type
杂志文章,评审相关文献
PROTEOMICS文献大全abstract::Several lines of evidence suggest that hnRNPs A/B (hnRNPs A1, A2/B1, and A3) play an important role in proliferation, although the functional overlap among members of hnRNPs A/B remains largely unknown. In this study, we have employed RNAi knockdown and proteomic approaches to investigate the biological functions of h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300549
更新日期:2014-06-01 00:00:00
abstract::The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this st...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600417
更新日期:2017-09-01 00:00:00
abstract::Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human thyroid cells were investigated ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200070
更新日期:2012-08-01 00:00:00
abstract::We here present a user-friendly and extremely lightweight tool that can serve as a stand-alone front-end for the Open MS Search Algorithm (OMSSA) search engine, or that can directly be used as part of an informatics processing pipeline for MS driven proteomics. The OMSSA graphical user interface (OMSSAGUI) tool is wri...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701126
更新日期:2008-06-01 00:00:00
abstract::The useful structural features of class II MHC molecules are rarely integrated into T-cell epitope predictions. We propose an approach that applies a novel expectation-maximization algorithm to align the naturally processed peptides selected by the class II MHC I-A(g7) molecule - focusing on the five MHC-specific anch...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600584
更新日期:2007-02-01 00:00:00
abstract::Myogenesis is a complex process of which the underlying mechanisms are conserved between species, including birds and mammals. Despite a good understanding of the stages of myogenesis, many of the mechanisms involved in the regulation of proliferation of the successive myoblast generations, the cellular transitions ce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701101
更新日期:2008-10-01 00:00:00
abstract::The quantification of changes in protein abundance in complex biological specimens is essential for proteomic studies in basic and applied research. Here we report on the development and validation of the DeepQuanTR software for identification and quantification of differentially expressed proteins using LC-MALDI-MS. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900634
更新日期:2010-07-01 00:00:00
abstract::Cells have a rich inner structure that is commonly explored by microscopy. Classical biochemical methods that break apart the cells and fractionate them along a gradient have now gotten a new lease on life through modern methods of mass spectrometry-based proteomics. Their common principle is to comprehensively measur...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900330
更新日期:2020-08-03 00:00:00
abstract::Off-line coupling of capillary IEF (CIEF) with matrix-assisted laser desorption/ionization mass spectrometry was utilized for the analysis of human blood serum. Serum proteins were initially separated by CIEF, and fractions of the isoelectric separation were eluted sequentially to a MALDI-TOF MS sample target. During ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401212
更新日期:2005-09-01 00:00:00
abstract::We report on a novel strategy for identification of specific sulfation motifs in chondroitin/dermatan sulfate (CS/DS) chain derived from decorin (Dcn), based on enzyme cleavage and multistage MS (MS(n)). Released CS/DS chains were digested with chondroitin B and in parallel with AC I lyases to obtain oligosaccharides ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800440
更新日期:2009-07-01 00:00:00
abstract::The PIK3CA gene encodes for the p110 alpha isoform of PI3 kinase and is one of the most frequently mutated oncogenes in human cancers. However, the mechanisms by which PIK3CA mutations activate cell signaling are not fully understood. Here we used a phosphoproteomic approach to compare differential phosphorylation pat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400342
更新日期:2015-01-01 00:00:00
abstract::The spring workshop of the HUPO-PSI convened in Siena to further progress the data standards which are already making an impact on data exchange and deposition in the field of proteomics. Separate work groups pushed forward existing XML standards for the exchange of Molecular Interaction data (PSI-MI, MIF) and Mass Sp...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200500626
更新日期:2005-09-01 00:00:00
abstract::Extracellular vesicles (EVs) are lipid-bilayered vesicles that are released by multiple cell types and contain nucleic acids and proteins. Very little is known about how the cargo is packaged into EVs. Ubiquitination of proteins is a key posttranslational modification that regulates protein stability and trafficking t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800266
更新日期:2018-09-01 00:00:00
abstract::The tuber of potato (Solanum tuberosum) is commonly used as a model for underground storage organs. In this study, changes in the proteome were followed from tuberization, through tuber development and storage into the sprouting phase. Data interrogation using principal component analysis was able to clearly discrimin...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600383
更新日期:2006-11-01 00:00:00
abstract::O-GlcNAcylation is a dynamic PTM of nuclear and cytoplasmic proteins, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase, which catalyze the addition and removal of O-GlcNAc, respectively. This modification is associated with glucose metabolism, which plays important roles in many diseases including cancer. Altho...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200126
更新日期:2013-07-01 00:00:00
abstract::A method is introduced to evaluate protein concentrations using the height sum of all MALDI-MS peaks that unambiguously match theoretic tryptic peptide masses of the protein sought after. The method uses native chromatographic protein fractionation prior to digestion but does not require any depletion, labeling, deriv...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500747
更新日期:2006-07-01 00:00:00
abstract::The Japanese red and pink corals are known to be precious because of their commercial value resulting from their use in ornaments, jewelry, and medicine. Precious corals are very interesting models for biomineralization studies and possess two different skeletal structures: an axial skeleton and an endoskeleton (scler...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300519
更新日期:2014-11-01 00:00:00
abstract::Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900502
更新日期:2010-02-01 00:00:00
abstract::In recent years, a new paradigm for genome annotation has emerged, termed "proteogenomics," that leverages peptide MS to annotate a genome. This is achieved by mapping peptides to a six-frame translation of a genome, including available splice databases, which may suggest refinements to gene models. Using this approac...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400185
更新日期:2014-12-01 00:00:00
abstract::Protein phosphorylation on serine, threonine, and tyrosine is known to be involved in a wide variety of cellular processes, signal transduction, and bacterial virulence. We characterized, for the first time, the extracellular phosphoproteins of the Pseudomonas aeruginosa PA14 strain. We identified 28 phosphoproteins (...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400190
更新日期:2014-09-01 00:00:00
abstract::Plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. Some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergisti...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200048
更新日期:2012-08-01 00:00:00
abstract::We monitored rates of growth, ATP-synthesis, respiration, and death to assess the sensitivity of the model organism Pseudomonas putida KT2440 to methyl tert-butyl ether (MTBE), and its degree of toxicity. The physiological data obtained suggested that the energy conservation system was the most sensitive site. However...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300477
更新日期:2003-08-01 00:00:00
abstract::The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200601019
更新日期:2007-09-01 00:00:00
abstract::Precise protein quantification is essential in comparative proteomics. Currently, quantification bias is inevitable when using proteotypic peptide-based quantitative proteomics strategy for the differences in peptides measurability. To improve quantification accuracy, we proposed an "empirical rule for linearly correl...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300032
更新日期:2014-07-01 00:00:00
abstract::To exploit advances in proteomics for drug discovery, high-throughout methods for target validation that directly address the cellular roles of proteins are required. To do this, we have characterized fluorophore-assisted light inactivation (FALI) which uses coherent or diffuse light targeted by fluorescein-labeled pr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200203)2:3<247::aid-prot247>3.0.
更新日期:2002-03-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is the second cause of cancer-related deaths worldwide. A clearer understanding of the molecular mechanisms underlying tumor growth and invasiveness remains crucial for developing new therapies. Here, the expression of tetraspanins, a family of plasma membrane organizers involved in tumo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900025
更新日期:2019-11-01 00:00:00
abstract::Total parenteral nutrition (TPN) is provided as the primary nitrogen source to manage patients with intestinal failure who were not able to sustain themselves on enteral feeds. The most common complication of long-term TPN use is hepatitis. A proteomic approach was used to identify proteins that are differentially exp...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500128
更新日期:2015-11-01 00:00:00
abstract::Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200356
更新日期:2013-03-01 00:00:00
abstract::Candida glabrata is a fungal pathogen that causes a variety of mucosal and systemic infections among compromised patient populations with higher mortality rates. Previous studies have shown that biofilm mode of the growth of the fungus is highly resistant to antifungal agents compared with the free-floating or plankto...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900611
更新日期:2010-04-01 00:00:00
abstract::The CpG motif of bacterial DNA (CpG-DNA) is a potent immunostimulating agent whose mechanism of action is not yet clear. Here, we used both DNA microarray and proteomic approaches to investigate the effects of oligodeoxynucleotides containing the CpG motif (CpG-ODN) on gene transcription and protein expression profile...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401144
更新日期:2005-03-01 00:00:00