In vivo proteome dynamics during early bovine myogenesis.

abstract：:Most MS-based glycomic and glycoproteomic analyses focus on identifying changes in terminal glyco-epitopes represented by sialylation and fucosylation at specific positions of the terminal N-acetyllactosamine units. Much less attention was accorded to the underlying linear or branched poly-N-acetyllactosamine extensio...

journal_title：Proteomics

authors： Wang SH,Wu SW,Khoo KH

更新日期：2011-07-01 00:00:00

abstract：:The explosion of site- and context-specific in vivo phosphorylation events presents a potentially rich source of biological knowledge and calls for novel data analysis and modeling paradigms. Perhaps the most immediate challenge is delineating detected phosphorylation sites to their effector kinases. This is important...

journal_title：Proteomics

authors： Tan CS,Linding R

更新日期：2009-12-01 00:00:00

abstract：:Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high-throughput ...

journal_title：Proteomics

authors： Dam S,Dyrlund TF,Ussatjuk A,Jochimsen B,Nielsen K,Goffard N,Ventosa M,Lorentzen A,Gupta V,Andersen SU,Enghild JJ,Ronson CW,Roepstorff P,Stougaard J

更新日期：2014-02-01 00:00:00

abstract：:Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage ...

journal_title：Proteomics

authors： Franco CF,Santos R,Coelho AV

更新日期：2011-09-01 00:00:00

abstract：:We present here the first proteomics analysis of uveal melanoma (UM) cells. These cells represent a good model for the identification of polypeptide markers, which could be developed as diagnostic tools. UM is the most common primary intraocular tumour in adults. In contrast to other cancers, the survival rate of pati...

journal_title：Proteomics

authors： Pardo M,García A,Thomas B,Piñeiro A,Akoulitchev A,Dwek RA,Zitzmann N

更新日期：2005-12-01 00:00:00

abstract：:"Liquid biopsies" have received attention as a complementary tool for traditional tissue biopsies that may enhance the spectrum of analysis for tumor-derived factors. One such factor gaining prominence in the liquid biopsy field is extracellular vesicles (EVs), membrane-bound nanovesicles which are secreted by cells i...

journal_title：Proteomics

authors： Ruhen O,Meehan K

更新日期：2019-01-01 00:00:00

abstract：:The main goal of many proteomics experiments is an accurate and rapid quantification and identification of regulated proteins in complex biological samples. The bottleneck in quantitative proteomics remains the availability of efficient software to evaluate and quantify the tremendous amount of mass spectral data acqu...

journal_title：Proteomics

authors： Brunner A,Keidel EM,Dosch D,Kellermann J,Lottspeich F

更新日期：2010-01-01 00:00:00

abstract：:Cofilin, an actin-binding protein, regulates the rate, nature and extent of assembly of the actin cytoskeleton. Native Phast gels show that the addition of cofilin to an actin-DNase I complex (74 kDa) results in the formation of a ternary complex of 94 kDa indicating an equimolar stoichiometry in the ternary complex. ...

journal_title：Proteomics

authors： Nosworthy NJ,Kekic M,dos Remedios CG

更新日期：2001-12-01 00:00:00

abstract：:Total parenteral nutrition (TPN) is provided as the primary nitrogen source to manage patients with intestinal failure who were not able to sustain themselves on enteral feeds. The most common complication of long-term TPN use is hepatitis. A proteomic approach was used to identify proteins that are differentially exp...

journal_title：Proteomics

authors： Tsai JJ,Kuo HC,Lee KF,Tsai TH

更新日期：2015-11-01 00:00:00

abstract：:MALDI-MS Imaging is a novel label-free technique that can be used to visualize the changes in multiple mass responses following treatment. Following treatment with proinflammatory cytokine interleukin-22 (IL-22), the epidermal differentiation of Labskin, a living skin equivalent (LSE), successfully modeled psoriasis i...

journal_title：Proteomics

authors： Harvey A,Cole LM,Day R,Bartlett M,Warwick J,Bojar R,Smith D,Cross N,Clench MR

更新日期：2016-06-01 00:00:00

abstract：:As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restr...

journal_title：Proteomics

authors： Ge P,Hao P,Cao M,Guo G,Lv D,Subburaj S,Li X,Yan X,Xiao J,Ma W,Yan Y

更新日期：2013-10-01 00:00:00

abstract：:Panax ginseng is an important herb that has clear effects on the treatment of diverse diseases. Until now, the natural peptide constitution of this herb remains unclear. Here, we conduct an extensive characterization of Ginseng peptidome using MS-based data mining and sequencing. The screen on the charge states of pre...

journal_title：Proteomics

authors： Ye X,Zhao N,Yu X,Han X,Gao H,Zhang X

更新日期：2016-11-01 00:00:00

abstract：:A low virulent Candida albicans mutant, CNC13, deleted in the Mitogen Activated Protein (MAP) kynase HOG1 was used to immunize BALB/c mice. Hog1p is essential for the oxidative stress and hyperosmolarity responses. Several doses and immunization procedures were employed. The protection capacity of the different sera g...

journal_title：Proteomics

authors： Fernández-Arenas E,Molero G,Nombela C,Diez-Orejas R,Gil C

更新日期：2004-04-01 00:00:00

abstract：:Off-line coupling of capillary IEF (CIEF) with matrix-assisted laser desorption/ionization mass spectrometry was utilized for the analysis of human blood serum. Serum proteins were initially separated by CIEF, and fractions of the isoelectric separation were eluted sequentially to a MALDI-TOF MS sample target. During ...

journal_title：Proteomics

authors： Crowley TA,Hayes MA

更新日期：2005-09-01 00:00:00

abstract：:Mitochondria consist of four compartments, outer membrane, intermembrane space, inner membrane, and matrix; each harboring specific functions and structures. In this study, we used LC-MS/MS to characterize the protein composition of Trypanosoma brucei mitochondrial (mt) membranes, which were enriched by different bioc...

journal_title：Proteomics

authors： Acestor N,Panigrahi AK,Ogata Y,Anupama A,Stuart KD

更新日期：2009-12-01 00:00:00

abstract：:CREC proteins constitute a family of EF-hand calcium binding proteins localized to the secretory pathway. Calumenin is the only member known to be secreted. Recently, it was shown that thrombin-activated thrombocytes liberate calumenin, which also is found in atherosclerotic lesions but not in normal vasculature. To s...

journal_title：Proteomics

authors： Ostergaard M,Hansen GA,Vorum H,Honoré B

更新日期：2006-06-01 00:00:00

abstract：:Abnormalities of the anterior cingulate cortex have previously been described in schizophrenia, major depressive disorder and bipolar disorder. In this study 2-DE was performed followed by mass spectrometric sequencing to identify disease-specific protein changes within the anterior cingulate cortex in these psychiatr...

journal_title：Proteomics

authors： Beasley CL,Pennington K,Behan A,Wait R,Dunn MJ,Cotter D

更新日期：2006-06-01 00:00:00

abstract：:Aging is associated with a progressive loss of skeletal muscular function that often leads to progressive disability and loss of independence. Although muscle aging is well documented, the molecular mechanisms of this condition still remain unclear. To gain greater insight into the changes associated with aging of ske...

journal_title：Proteomics

authors： Hwang CY,Kim K,Choi JY,Bahn YJ,Lee SM,Kim YK,Lee C,Kwon KS

更新日期：2014-01-01 00:00:00

abstract：:This study describes the gel-free phosphoproteomic analysis of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea grown in vitro under nonlimiting conditions. Using a combination of strong cation exchange and IMAC prior to LC-MS, we identified over 1350 phosphopeptides per fungus representing over ...

journal_title：Proteomics

authors： Davanture M,Dumur J,Bataillé-Simoneau N,Campion C,Valot B,Zivy M,Simoneau P,Fillinger S

更新日期：2014-07-01 00:00:00

abstract：:Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate...

journal_title：Proteomics

authors： Molle V,Leiba J,Zanella-Cléon I,Becchi M,Kremer L

更新日期：2010-11-01 00:00:00

abstract：:Proteomic analysis on sperm has been restricted to only a few model organisms. We present here a 2DE PAGE proteome map of sperm cells from a nonmodel organism, the marine mussel Mytilus edulis, a free-spawning marine invertebrate with external fertilization. Ninety-six protein spots showing high expression were select...

journal_title：Proteomics

authors： Diz AP,Dudley E,Skibinski DO

更新日期：2012-06-01 00:00:00

abstract：:The characterization of peptides bound to human leukocyte antigen (HLA) class I is of fundamental importance for understanding CD8+ T cell-driven immunological processes and for the development of immunomodulatory therapeutic strategies. However, until now, the mass spectrometric analysis of HLA-bound peptides has typ...

journal_title：Proteomics

authors： Ritz D,Gloger A,Weide B,Garbe C,Neri D,Fugmann T

更新日期：2016-05-01 00:00:00

abstract：:Voltage-gated Kv7.2 potassium channels regulate neuronal excitability. The gating of these channels is tightly controlled by various mediators and neurotransmitters acting via G protein-coupled receptors; the underlying signaling cascades involve phosphatidylinositol-4,5-bisphosphate (PIP2 ), Ca2+ /calmodulin, and pho...

journal_title：Proteomics

authors： Erdem FA,Salzer I,Heo S,Chen WQ,Jung G,Lubec G,Boehm S,Yang JW

更新日期：2017-10-01 00:00:00

abstract：:Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro-organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance ...

journal_title：Proteomics

authors： Christie-Oleza JA,Scanlan DJ,Armengaud J

更新日期：2015-10-01 00:00:00

abstract：:The senescence-accelerated mouse (SAM) is a useful animal model to study aging or age-associated disorders due to its inherited aging phenotype. To investigate proteins involved in the aging process in liver, we compared the young (4- or 20-week old) and the aged group (50-week-old) of SAMP8 (short life span) and SAMR...

journal_title：Proteomics

authors： Cho YM,Bae SH,Choi BK,Cho SY,Song CW,Yoo JK,Paik YK

更新日期：2003-10-01 00:00:00

abstract：:High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effec...

journal_title：Proteomics

authors： Hörmann S,Scheyhing C,Behr J,Pavlovic M,Ehrmann M,Vogel RF

更新日期：2006-03-01 00:00:00

abstract：:Brain development and aging is a complex process involving proliferation, differentiation and apoptosis. Elucidating proteome changes in these processes can help to understand the mechanisms of brain development and maintenance as well as neurodegenerative diseases. The research reported here is a contribution to the ...

journal_title：Proteomics

authors： Föcking M,Boersema PJ,O'Donoghue N,Lubec G,Pennington SR,Cotter DR,Dunn MJ

更新日期：2006-09-01 00:00:00

abstract：:Identification and analysis of types of biological protein-protein interactions and their interfaces to predict obligate and non-obligate complexes is a problem that has drawn the attention of the research community in the past few years. In this paper, we propose a prediction approach to predict these two types of co...

journal_title：Proteomics

authors： Aziz MM,Maleki M,Rueda L,Raza M,Banerjee S

更新日期：2011-10-01 00:00:00

abstract：:The transfection of glycoprotein glycosyltransferase genes into cells leads to modification of both the structure and function of the glycoproteins and as a result, changes in glycome patterns. N-glycan branching enzymes hold some promise as a model system for the identification of glycome patterns. Both N-acetylgluco...

journal_title：Proteomics

authors： Taniguchi N,Ekuni A,Ko JH,Miyoshi E,Ikeda Y,Ihara Y,Nishikawa A,Honke K,Takahashi M

更新日期：2001-02-01 00:00:00

abstract：:Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...

journal_title：Proteomics

authors： Corrigan L,Jefferies C,Clive Lee T,Daly J

更新日期：2011-08-01 00:00:00