Abstract:
:MALDI-MS Imaging is a novel label-free technique that can be used to visualize the changes in multiple mass responses following treatment. Following treatment with proinflammatory cytokine interleukin-22 (IL-22), the epidermal differentiation of Labskin, a living skin equivalent (LSE), successfully modeled psoriasis in vitro. Masson's trichrome staining enabled visualization and quantification of epidermal differentiation between the untreated and IL-22 treated psoriatic LSEs. Matrix-assisted laser desorption ionization mass spectrometry imaging was used to observe the spatial location of the psoriatic therapy drug acetretin following 48 h treatments within both psoriatic and normal LSEs. After 24 h, the drug was primarily located in the epidermal regions of both the psoriatic and nonpsoriatic LSE models whereas after 48 h it was detectible in the dermis.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Harvey A,Cole LM,Day R,Bartlett M,Warwick J,Bojar R,Smith D,Cross N,Clench MRdoi
10.1002/pmic.201600036subject
Has Abstractpub_date
2016-06-01 00:00:00pages
1718-25issue
11-12eissn
1615-9853issn
1615-9861journal_volume
16pub_type
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