Cobalt Phthalocyanine Tetracarboxylic Acid Functionalized Polymer Monolith for Selective Enrichment of Glycopeptides and Glycans.

abstract：:The function of a protein is modulated by its abundance and its degree of specific post-translational modifications such as phosphorylation, glycosylation or truncation. Consequently, changes of protein concentration and the extent of their post-translational modifications has a great influence on the activity of intr...

journal_title：Proteomics

authors： Moritz B,Meyer HE

更新日期：2003-11-01 00:00:00

abstract：:Neurons present a highly polarized morphology, often displaying a significantly imbalanced distribution of the cytoplasm between the somatic and axonal domains. This imbalance requires cell-specific mechanisms for the maintenance of the axoplasmic mass during development, neuronal homeostasis, and recovery after injur...

journal_title：Proteomics

authors： Farias J,Sotelo JR,Sotelo-Silveira J

更新日期：2019-08-01 00:00:00

abstract：:Transcription factors (TFs) are a family of DNA-binding proteins whose gene regulatory capabilities are of vital importance in defining the molecular state of a cell. Despite their biological significance, our understanding of TF behavior and function is still limited. This is because we have so far mostly relied on g...

journal_title：Proteomics

authors： Simicevic J,Deplancke B

更新日期：2017-02-01 00:00:00

abstract：:In recent years, proteomics research has gained importance due to increasingly powerful techniques in protein purification, mass spectrometry and identification, and due to the development of extensive protein and DNA databases from various organisms. Nevertheless, current identification methods from spectrometric dat...

journal_title：Proteomics

authors： Hernandez P,Gras R,Frey J,Appel RD

更新日期：2003-06-01 00:00:00

abstract：:Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional, immunological and structural aspects of both eukaryotic and prokaryotic glycoproteins. Traditional strategies used to analyse glycoprotein O-glycans involve glycoprotein isolation, followed by glycan release using solu...

journal_title：Proteomics

authors： Taylor AM,Holst O,Thomas-Oates J

更新日期：2006-05-01 00:00:00

abstract：:Protein quantification at proteome-wide scale is an important aim, enabling insights into fundamental cellular biology and serving to constrain experiments and theoretical models. While proteome-wide quantification is not yet fully routine, many datasets approaching proteome-wide coverage are becoming available throug...

journal_title：Proteomics

authors： Wang M,Herrmann CJ,Simonovic M,Szklarczyk D,von Mering C

更新日期：2015-09-01 00:00:00

abstract：:Lysine crotonylation (Kcr) is a recently discovered post-translational modification that potentially regulates multiple biological processes. With an objective to expand the available crotonylation datasets, LC-MS/MS is performed using mouse liver samples under normal physiological conditions to obtain in vivo crotony...

journal_title：Proteomics

authors： Liu JF,Wu SF,Liu S,Sun X,Wang XM,Xu P,Chen HZ,Yang JT

更新日期：2020-10-01 00:00:00

abstract：:Sporozoites of the malaria parasite Plasmodium are transmitted by mosquitoes and infect the liver for an initial and obligatory round of replication, before exponential multiplication in the blood and onset of the disease. Sporozoites and liver stages provide attractive targets for malaria vaccines and prophylactic dr...

journal_title：Proteomics

authors： Hamada S,Pionneau C,Parizot C,Silvie O,Chardonnet S,Marinach C

更新日期：2021-01-16 00:00:00

abstract：:Green tea polyphenols exhibit multiple antitumor activities, and the mechanisms of action are not completely understood. Previously, we reported that green tea extract (GTE)-induced actin remolding is associated with increased cell adhesion and decreased motility in A549 lung cancer cells. To identify the cellular tar...

journal_title：Proteomics

authors： Lu QY,Yang Y,Jin YS,Zhang ZF,Heber D,Li FP,Dubinett SM,Sondej MA,Loo JA,Rao JY

更新日期：2009-02-01 00:00:00

abstract：:LTQ Orbitrap data analyzed with ProteinPilot can be further improved by MaxQuant raw data processing, which utilizes precursor-level high mass accuracy data for peak processing and MGF creation. In particular, ProteinPilot results from MaxQuant-processed peaklists for Orbitrap data sets resulted in improved spectral u...

journal_title：Proteomics

authors： Jagtap P,Bandhakavi S,Higgins L,McGowan T,Sa R,Stone MD,Chilton J,Arriaga EA,Seymour SL,Griffin TJ

更新日期：2012-06-01 00:00:00

abstract：:The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...

journal_title：Proteomics

authors： Marengo E,Robotti E,Antonucci F,Cecconi D,Campostrini N,Righetti PG

更新日期：2005-02-01 00:00:00

abstract：:Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at ...

journal_title：Proteomics

authors： Rampitsch C,Günel A,Beimcik E,Mauthe W

更新日期：2015-04-01 00:00:00

abstract：:Proteins determine the quality of barley in malting and brewing end-uses. In this regard, water-soluble barley proteins play a major role in the formation, stability, and texture of head foams. Our objective was to survey the barley seed proteins that could be involved in the foaming properties of beer. Therefore, two...

journal_title：Proteomics

authors： Perrocheau L,Rogniaux H,Boivin P,Marion D

更新日期：2005-07-01 00:00:00

abstract：:The lipid droplet (LD) is an organelle with vital functions found in nearly all organisms. LD proteomic research has provided fundamentally important insights into this organelle's functions. The review provides a summary of LD proteomic studies conducted across diverse organisms and cell and tissue types. The accumul...

journal_title：Proteomics

authors： Zhang C,Liu P

更新日期：2019-05-01 00:00:00

abstract：:Despite affecting up to 70% of HIV-positive patients and being the leading cause of dementia in patients under 40 years, the molecular mechanisms involved in the onset of HIV-associated neurocognitive disorders (HAND) are not well understood. To address this, we performed SILAC-based quantitative proteomic analysis on...

journal_title：Proteomics

authors： Ganief T,Gqamana P,Garnett S,Hoare J,Stein DJ,Joska J,Soares N,Blackburn JM

更新日期：2017-03-01 00:00:00

abstract：:The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...

journal_title：Proteomics

authors： Tully B,Balleine RL,Hains PG,Zhong Q,Reddel RR,Robinson PJ

更新日期：2019-11-01 00:00:00

abstract：:Human galectin-3 (hGal-3) is a mammalian lectin involved in regulation of RNA splicing, apoptosis, cell differentiation, and proliferation. Multimerized extracellular hGal-3 is thought to crosslink cells by binding to glycoproteins and glycosylated cancer antigens on the cell surface or extracellular matrix. Fluoresce...

journal_title：Proteomics

authors： Bogoeva VP,Varriale A,John CM,D'Auria S

更新日期：2010-05-01 00:00:00

abstract：:Rhodococcus sp. strain TFB is a versatile gram-positive bacterium able to grow on a wide variety of aromatic compounds as carbon and energy sources. Since the strain is refractory to genetic analysis, a proteomic approach was used to study the metabolic pathways involved in the catabolism of such compounds by analyzin...

journal_title：Proteomics

authors： Tomás-Gallardo L,Canosa I,Santero E,Camafeita E,Calvo E,López JA,Floriano B

更新日期：2006-04-01 00:00:00

abstract：:FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...

journal_title：Proteomics

authors： Gattiker A,Bienvenut WV,Bairoch A,Gasteiger E

更新日期：2002-10-01 00:00:00

abstract：:Trophoblastic cell lines are widely used in in vitro studies of placental function as a surrogate for primary trophoblasts. To date, no reference proteomics dataset exists to directly compare the shared and unique characteristics of these cells. Here, we performed comparative proteomic profiling of the BeWo and HTR8/S...

journal_title：Proteomics

authors： Szklanna PB,Wynne K,Nolan M,Egan K,Áinle FN,Maguire PB

更新日期：2017-05-01 00:00:00

abstract：:More than 300 different types of protein post-translational modifications (PTMs) have been described, many of which are known to have pivotal roles in cellular physiology and disease. Nevertheless, only a handful of PTMs have been extensively investigated at the proteome level. Knowledge of protein substrates and thei...

journal_title：Proteomics

authors： Zhao Y,Jensen ON

更新日期：2009-10-01 00:00:00

abstract：:In the postgenomic era, many researchers and organizations have been engaged in structural and functional analyses of proteins. As a part of these efforts, searching for small organic compounds that bind specifically to target proteins is quite important. In this study, we have developed a rational strategy for ligand...

journal_title：Proteomics

authors： Yoshitani N,Satou K,Saito K,Suzuki S,Hatanaka H,Seki M,Shinozaki K,Hirota H,Yokoyama S

更新日期：2005-04-01 00:00:00

abstract：:We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chrom...

journal_title：Proteomics

authors： Baek MC,Kim SJ,Yea K,Kim Y,Lee BD,Kim J,Lee HJ,Kang MH,Choi SK,Kim JI,Lee TG,Suh PG,Ryu SH

更新日期：2006-03-01 00:00:00

abstract：:Bottom-up proteomics is a mass spectrometric (MS)-based approach for the characterization of peptides obtained from in-solution protein digestion. MS is favored over other methods for peptide and protein analysis because of its better sensitivity and high throughput. Inorganic ions and surfactants present in the sampl...

journal_title：Proteomics

authors： Tubaon RM,Haddad PR,Quirino JP

更新日期：2017-10-01 00:00:00

abstract：:Cardiac conduction defects were found in transgenic mice deficient in urea transporter UT-B. To investigate the molecular mechanisms of the conduction defects caused by UT-B deletion, we studied the protein expression profiles of heart tissue (comprising most conduction system) in wild-type versus UT-B null mice at di...

journal_title：Proteomics

authors： Yu H,Meng Y,Wang LS,Jin X,Gao LF,Zhou L,Ji K,Li Y,Zhao LJ,Chen GQ,Zhao XJ,Yang B

更新日期：2009-02-01 00:00:00

abstract：:During the peri-implantation period, molecular signaling between embryo and endometrium (layer of tissue lining the uterus lumen) is supposed to be crucial for the maintenance of pregnancy. To investigate embryo-induced alterations in the proteome of bovine endometrium in the preattachment period (day 18), we used mon...

journal_title：Proteomics

authors： Berendt FJ,Fröhlich T,Schmidt SE,Reichenbach HD,Wolf E,Arnold GJ

更新日期：2005-07-01 00:00:00

abstract：:As a consequence of their poor solubility during isoelectric focusing, integral membrane proteins are generally absent from two-dimensional gel proteome maps. In order to analyze the yeast plasma membrane proteome, a plasma membrane purification protocol was optimized in order to reduce contaminating membranes and cyt...

journal_title：Proteomics

authors： Navarre C,Degand H,Bennett KL,Crawford JS,Mørtz E,Boutry M

更新日期：2002-12-01 00:00:00

abstract：:Lectin microarray is an emerging technique, which will accelerate glycan profiling and discovery of glycan-related biomarkers. One of the most important stages in realizing the potential of the technique is to achieve sufficiently high sensitivity to detect even the low concentrations of some target glycoproteins whic...

journal_title：Proteomics

authors： Uchiyama N,Kuno A,Tateno H,Kubo Y,Mizuno M,Noguchi M,Hirabayashi J

更新日期：2008-08-01 00:00:00

abstract：:Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...

journal_title：Proteomics

authors： Ahrné E,Müller M,Lisacek F

更新日期：2010-02-01 00:00:00

abstract：:Mitochondria play a key role as major regulators of cellular energy homeostasis, but in the context of mitochondrial dysfunction, mitochondria may generate reactive oxidative species and induce cellular apoptosis. Indeed, altered mitochondrial status has been linked to the pathogenesis of several metabolic disorders a...

journal_title：Proteomics

authors： Peinado JR,Diaz-Ruiz A,Frühbeck G,Malagon MM

更新日期：2014-03-01 00:00:00