Abstract:
:Green tea polyphenols exhibit multiple antitumor activities, and the mechanisms of action are not completely understood. Previously, we reported that green tea extract (GTE)-induced actin remolding is associated with increased cell adhesion and decreased motility in A549 lung cancer cells. To identify the cellular targets responsible for green tea-induced actin remodeling, we performed 2-DE LC-MS/MS of A549 cells before and after GTE exposure. We have identified 14 protein spots that changed in expression (> or =2-fold) after GTE treatment. These proteins are involved in calcium-binding, cytoskeleton and motility, metabolism, detoxification, or gene regulation. In particular we found upregulation of several genes that modulate actin remodeling and cell migration, including lamin A/C. Our data indicated that GTE-induced lamin A/C upregulation appears to be at the transcriptional level and the increased expression results in the decrease in cell motility, as confirmed by siRNA. The result of the study demonstrates that GTE alters the levels of many proteins involved in growth, motility and apoptosis of A549 cells and their identification may explain the multiple antitumor activities of GTE.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Lu QY,Yang Y,Jin YS,Zhang ZF,Heber D,Li FP,Dubinett SM,Sondej MA,Loo JA,Rao JYdoi
10.1002/pmic.200800019subject
Has Abstractpub_date
2009-02-01 00:00:00pages
757-67issue
3eissn
1615-9853issn
1615-9861journal_volume
9pub_type
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