Abstract:
:Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to alleviate comigration associated drawbacks. Spot resolution is strikingly improved following this simple and rapid method and the positive impact on protein and peptide identification from MS/MS data, on the analysis of relative changes in protein abundance, and on the detection of PTM is described.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Colignon B,Raes M,Dieu M,Delaive E,Mauro Sdoi
10.1002/pmic.201200494subject
Has Abstractpub_date
2013-07-01 00:00:00pages
2077-82issue
14eissn
1615-9853issn
1615-9861journal_volume
13pub_type
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