Abstract:
:In-gel peptide digestion has become a widely used technique for characterizing proteins resolved by two-dimensional gel electrophoresis. Peptides generated from gel pieces are frequently contaminated with detergent and salts. Prior to matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysis, these contaminants are removed using micro scale C18 sample preparation columns. In this paper, data are presented to demonstrate the application of a solvent resistant MultiScreen 96-well plate with a low peptide binding membrane and ZipTip micropipette based sample preparation. Recoveries of peptides (m/z of 1000 to 5000 Da) derived from standard protein protease digests, were estimated at various stages of the analytical process. An optimized protocol has been established and all the reagents and consumables have been packaged in a ready to use commercial kit. Data will be presented to show the application of this technology package to accelerate the throughput of protein characterization by protease fragmentation.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Pluskal MG,Bogdanova A,Lopez M,Gutierrez S,Pitt AMdoi
10.1002/1615-9861(200202)2:2<145::aid-prot145>3.0.subject
Has Abstractpub_date
2002-02-01 00:00:00pages
145-50issue
2eissn
1615-9853issn
1615-9861pii
10.1002/1615-9861(200202)2:2<145::AID-PROT145>3.0.journal_volume
2pub_type
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