Abstract:
:This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused on protein identification using the bottom-up shotgun approach. Protein extracts derived from both samples were trypsinized and analyzed separately by 2-D LC and MS. In a complementary approach, the tryptic digests were analyzed directly by LC-ESI-MS/MS and gas-phase fractionation in the mass spectrometer. Taken together, both proteomic approaches in combination with a stringent evaluation process, resulted in the confident identification of 209 proteins in the human brain samples under investigation.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Dumont D,Noben JP,Verhaert P,Stinissen P,Robben Jdoi
10.1002/pmic.200600080subject
Has Abstractpub_date
2006-09-01 00:00:00pages
4967-77issue
18eissn
1615-9853issn
1615-9861journal_volume
6pub_type
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