Single cell proteomics in biomedicine: High-dimensional data acquisition, visualization, and analysis.

abstract：:Protein complexes carry out almost the entire signaling and functional processes in the cell. The protein complex complement of a cell, and its network of complex-complex interactions, is referred to here as the complexome. Computational methods to predict protein complexes from proteomics data, resulting in network r...

journal_title：Proteomics

authors： Clancy T,Hovig E

更新日期：2014-01-01 00:00:00

abstract：:In this work, for the first time, perfluorinated magnetic mesoporous microspheres were designed and synthesized for the highly specific enrichment of fluorous-derivatized phosphopeptides through the unique fluorine-fluorine interactions. The perfluorinated magnetic mesoporous microspheres were prepared through a surfa...

journal_title：Proteomics

authors： Zhao M,Deng C

更新日期：2016-04-01 00:00:00

abstract：:High-throughput shotgun metaproteomic approaches on environmental or medical microbiomes are producing huge amounts of tandem mass spectrometry data. These can be interpreted either with a general protein sequence database comprising tens of thousands of sequenced genomes or with a more customized database such as tho...

journal_title：Proteomics

authors： Pible O,Armengaud J

更新日期：2015-10-01 00:00:00

abstract：:Intracellular serine protease-1 (Isp-1) is a major intracellular serine protease of Bacillus subtilis, whose functions still remain largely unknown. Furthermore, physiological substrates are yet to be determined. To identify Isp-1 substrates, we digested extract obtained from an Isp-1 deficient Bacillus mutant with pu...

journal_title：Proteomics

authors： Lee AY,Goo Park S,Kho CW,Young Park S,Cho S,Lee SC,Lee DH,Myung PK,Park BC

更新日期：2004-11-01 00:00:00

abstract：:Blood plasma is the most complex human-derived proteome, containing other tissue proteomes as subsets. This proteome has only been partially characterized due to the extremely wide dynamic range of the plasma proteins of more than ten orders of magnitude. Thus, the reduction in sample complexity prior to mass spectrom...

journal_title：Proteomics

authors： Nissum M,Kuhfuss S,Hauptmann M,Obermaier C,Sukop U,Wildgruber R,Weber G,Eckerskorn C,Malmström J

更新日期：2007-12-01 00:00:00

abstract：:Scab, caused by Fusarium graminearum, is a serious spike disease in wheat. To identify proteins in resistant wheat cultivar Wangshuibai induced by F. graminearum infection, proteins extracted from spikes 6, 12 and 24 h after inoculation were separated by 2-DE. Thirty protein spots showing 3-fold change in abundance wh...

journal_title：Proteomics

authors： Wang Y,Yang L,Xu H,Li Q,Ma Z,Chu C

更新日期：2005-11-01 00:00:00

abstract：:On 23 and 24 July, 2007, the Centre for Economic and Social Aspects of Genomics (CESAGen) held its first sociomics workshop at the Wellcome Trust Genome Campus, Hinxton, UK. The topic was transformation of knowledge production. Participants included social scientists together with those working on different elements o...

journal_title：Proteomics

authors： McNally R

更新日期：2008-01-01 00:00:00

abstract：:Realistic in vitro models are critical in the drug development process. In this study, a novel in vitro platform is employed to assess drug penetration and metabolism. This platform, which utilizes a 3D printed fluidic device, allows for dynamic dosing of three dimensional cell cultures, also known as spheroids. The p...

journal_title：Proteomics

authors： LaBonia GJ,Lockwood SY,Heller AA,Spence DM,Hummon AB

更新日期：2016-06-01 00:00:00

abstract：:Low levels and long term exposure to benzene is associated with hematotoxicity including aplastic anemia, acute myelogenous leukemia, and lymphoma. Current biomonitoring methods such as urinary phenol, S-phenylmercapturic acid, and trans-trans muconic acid were found to be unreliable as analytical methods to detect be...

journal_title：Proteomics

authors： Joo WA,Kang MJ,Son WK,Lee HJ,Lee DY,Lee E,Kim CW

更新日期：2003-12-01 00:00:00

abstract：:Top-down proteomics have recently started to gain attention as a novel method to provide insight into the structure of proteins in their native state, specifically the number and location of disulfide bridges. However, previous techniques still relied on complex and time-consuming protein purification and reduction re...

journal_title：Proteomics

authors： Khairallah RJ,Sadayappan S

更新日期：2013-11-01 00:00:00

abstract：:Adiponectin is a fat cell-secreted hormone with antidiabetic and anti-inflammatory activities. The reduced adiponectin levels are associated with obesity-related metabolic syndrome. Replenishment of this hormone into animal models can improve insulin sensitivity, decrease blood glucose and lipid levels, and prevent th...

journal_title：Proteomics

authors： Wang Y,Xu LY,Lam KS,Lu G,Cooper GJ,Xu A

更新日期：2006-07-01 00:00:00

abstract：:Despite increasing evidence of behavioural manipulation of their vectors by pathogens, the underlying mechanisms causing infected vectors to act in ways that benefit pathogen transmission remain enigmatic in most cases. Here, 2-D DIGE coupled with MS were employed to analyse and compare the head proteome of mosquitoes...

journal_title：Proteomics

authors： Lefevre T,Thomas F,Schwartz A,Levashina E,Blandin S,Brizard JP,Le Bourligu L,Demettre E,Renaud F,Biron DG

更新日期：2007-06-01 00:00:00

abstract：:Autosomal dominant tubulointerstitial kidney disease associated to the MUC1 gene (ADTKD-MUC1; formerly MCKD1) belongs to a heterogeneous group of rare hereditary kidney diseases that is prototypically caused by frameshift mutations in the MUC1 repeat domain. The mutant MUC1 (insC) lacks the transmembrane domaine, exhi...

journal_title：Proteomics

authors： Staubach S,Wenzel A,Beck BB,Rinschen MM,Müller S,Hanisch FG

更新日期：2018-04-01 00:00:00

abstract：:The spring workshop of the HUPO-PSI convened in Siena to further progress the data standards which are already making an impact on data exchange and deposition in the field of proteomics. Separate work groups pushed forward existing XML standards for the exchange of Molecular Interaction data (PSI-MI, MIF) and Mass Sp...

journal_title：Proteomics

authors： Orchard S,Hermjakob H,Taylor CF,Potthast F,Jones P,Zhu W,Julian RK Jr,Apweiler R

更新日期：2005-09-01 00:00:00

abstract：:Suberoylanilide hydroxamic acid (SAHA) is a potent histone deacetylase (HDAC) inhibitor. Inhibitors of HDACs are used in cancer therapy based on the role HDACs play in transcription by regulating chromatin compaction and non-histone proteins such as transcription factors. Profiling of HDAC expression is of interest in...

journal_title：Proteomics

authors： Fischer JJ,Michaelis S,Schrey AK,Diehl A,Graebner OY,Ungewiss J,Horzowski S,Glinski M,Kroll F,Dreger M,Koester H

更新日期：2011-10-01 00:00:00

abstract：:Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage ...

journal_title：Proteomics

authors： Franco CF,Santos R,Coelho AV

更新日期：2011-09-01 00:00:00

abstract：:CD28 delivers a co-stimulatory signal for T cell antigen receptor induced activation of T cells through a mechanism which remains mostly elusive to date. In order to try and gain insight into CD28 function, we therefore applied state-of-the-art mass spectrometric protein identification technology to the analysis of CD...

journal_title：Proteomics

authors： Heller M,Watts JD,Aebersold R

更新日期：2001-01-01 00:00:00

abstract：:The discovery of PTMs in proteins by MS requires nearly complete sequence coverage of the detected proteolytic peptides. Unfortunately, mass spectrometric analysis of the desired sequence fragments is often impeded due to low ionization efficiency and/or signal suppression in complex samples. When several lysine resid...

journal_title：Proteomics

authors： Markoutsa S,Bahr U,Papasotiriou DG,Häfner AK,Karas M,Sorg BL

更新日期：2014-03-01 00:00:00

abstract：:Identification of differential proteomic responses to cadmium (Cd) would provide a means for better understanding the survival mechanisms of the earthworm, Eisenia fetida, living in a Cd-polluted environment, and the stress responses can be assessed by ecotoxiptoromics approaches. Extracts of whole earthworm collected...

journal_title：Proteomics

authors： Wang X,Chang L,Sun Z,Zhang Y,Yao L

更新日期：2010-12-01 00:00:00

abstract：:A multiplexed fluorescence immunoassay using a novel planar waveguide technology-based microarray system, ZeptoMARK (Zeptosens), was developed to detect simultaneously urokinase-type plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1), and vascular endothelial growth factor (VEGF) in extracts of bre...

journal_title：Proteomics

authors： Weissenstein U,Schneider MJ,Pawlak M,Cicenas J,Eppenberger-Castori S,Oroszlan P,Ehret S,Geurts-Moespot A,Sweep FC,Eppenberger U

更新日期：2006-03-01 00:00:00

abstract：:A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...

journal_title：Proteomics

authors： Huelseweh B,Ehricht R,Marschall HJ

更新日期：2006-05-01 00:00:00

abstract：:Protein phosphorylation on serine, threonine, and tyrosine is known to be involved in a wide variety of cellular processes, signal transduction, and bacterial virulence. We characterized, for the first time, the extracellular phosphoproteins of the Pseudomonas aeruginosa PA14 strain. We identified 28 phosphoproteins (...

journal_title：Proteomics

authors： Ouidir T,Jarnier F,Cosette P,Jouenne T,Hardouin J

更新日期：2014-09-01 00:00:00

abstract：:Cnidarians are widely distributed basal metazoans that play an important role in the marine ecosystem. Their genetic diversity and dispersal depends on successful oogenesis, fertilization and embryogenesis. To understand the processes that lead to successful embryogenesis in these basal organisms, we conducted compara...

journal_title：Proteomics

authors： Levitan S,Sher N,Brekhman V,Ziv T,Lubzens E,Lotan T

更新日期：2015-12-01 00:00:00

abstract：:Proteomics is increasingly being used to understand enzyme expression and regulatory mechanisms involved in the accumulation of storage reserves in crops with sequenced genomes. During the past six years, considerable progress has been made to characterize proteomes of both mature and developing seeds, particularly oi...

journal_title：Proteomics

authors： Hajduch M,Matusova R,Houston NL,Thelen JJ

更新日期：2011-05-01 00:00:00

abstract：:Acute kidney injury (AKI) is one of the more frequent and lethal pathological conditions seen in intensive care units. Currently available treatments are not totally effective but stem cell-based therapies are emerging as promising alternatives, especially the use of mesenchymal stromal cells (MSC), although the signa...

journal_title：Proteomics

authors： da Costa MR,Pizzatti L,Lindoso RS,Sant'Anna JF,DuRocher B,Abdelhay E,Vieyra A

更新日期：2014-06-01 00:00:00

abstract：:A brief period of ischemia followed by timely reperfusion may lead to prolonged, yet reversible, contractile dysfunction (myocardial stunning). Damage to the myocardium occurs not only during ischemia, but also during reperfusion, where a massive release of oxygen-free radicals (OFR) occurs. We have previously utilize...

journal_title：Proteomics

authors： White MY,Tchen AS,McCarron HC,Hambly BD,Jeremy RW,Cordwell SJ

更新日期：2006-12-01 00:00:00

abstract：:Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A4...

journal_title：Proteomics

authors： Castagna A,Antonioli P,Astner H,Hamdan M,Righetti SC,Perego P,Zunino F,Righetti PG

更新日期：2004-10-01 00:00:00

abstract：:A novel strain of influenza A H1N1 emerged in the spring of 2009 and has spread rapidly throughout the world. Although vaccines have recently been developed that are expected to be protective, their availability was delayed until well into the influenza season. Although anti-influenza drugs such as neuraminidase inhib...

journal_title：Proteomics

authors： Chen CH,Zhang XQ,Lo CW,Liu PF,Liu YT,Gallo RL,Hsieh MF,Schooley RT,Huang CM

更新日期：2010-06-01 00:00:00

abstract：:A two-dimensional liquid-phase separation scheme coupled with mass spectrometry (MS) is presented for proteomic analysis of cell lysates from normal and malignant breast epithelial cell lines. Liquid-phase separations consist of isoelectric focusing as the first dimension and nonporous silica reverse-phase high-perfor...

journal_title：Proteomics

authors： Hamler RL,Zhu K,Buchanan NS,Kreunin P,Kachman MT,Miller FR,Lubman DM

更新日期：2004-03-01 00:00:00

abstract：:The characterization of peptides bound to human leukocyte antigen (HLA) class I is of fundamental importance for understanding CD8+ T cell-driven immunological processes and for the development of immunomodulatory therapeutic strategies. However, until now, the mass spectrometric analysis of HLA-bound peptides has typ...

journal_title：Proteomics

authors： Ritz D,Gloger A,Weide B,Garbe C,Neri D,Fugmann T

更新日期：2016-05-01 00:00:00