Characterization of a digested protein complex with quantitative aspects: an approach based on accurate mass chromatographic analysis with Fourier transform-ion cyclotron resonance mass spectrometry.

abstract：:Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...

journal_title：Proteomics

authors： Poot AJ,Ruijter E,Nuijens T,Dirksen EH,Heck AJ,Slijper M,Rijkers DT,Liskamp RM

更新日期：2006-12-01 00:00:00

abstract：:Antibiotics disturb the physiological homeostasis of bacterial cells by interfering with essential cellular functions or structures. The bacterial proteome adjusts quickly in response to antibiotic challenge. This physiological response is specifically tailored to overcome the inflicted damage and, thus, closely linke...

journal_title：Proteomics

authors： Wenzel M,Bandow JE

更新日期：2011-08-01 00:00:00

abstract：:Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at ...

journal_title：Proteomics

authors： Rampitsch C,Günel A,Beimcik E,Mauthe W

更新日期：2015-04-01 00:00:00

abstract：:Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic func...

journal_title：Proteomics

authors： Guergues J,Zhang P,Liu B,Stevens SM Jr

更新日期：2019-06-01 00:00:00

abstract：:Abnormalities of the anterior cingulate cortex have previously been described in schizophrenia, major depressive disorder and bipolar disorder. In this study 2-DE was performed followed by mass spectrometric sequencing to identify disease-specific protein changes within the anterior cingulate cortex in these psychiatr...

journal_title：Proteomics

authors： Beasley CL,Pennington K,Behan A,Wait R,Dunn MJ,Cotter D

更新日期：2006-06-01 00:00:00

abstract：:This review provides a brief overview of the development of data-independent acquisition (DIA) mass spectrometry-based proteomics and selected DIA data analysis tools. Various DIA acquisition schemes for proteomics are summarized first including Shotgun-CID, DIA, MSE , PAcIFIC, AIF, SWATH, MSX, SONAR, WiSIM, BoxCar, S...

journal_title：Proteomics

authors： Zhang F,Ge W,Ruan G,Cai X,Guo T

更新日期：2020-09-01 00:00:00

abstract：:Eukaryotic cells plasma membranes are organized into microdomains of specialized function such as lipid rafts and caveolae, with a specific lipid composition highly enriched in cholesterol and glycosphingolipids. In addition to their role in regulating signal transduction, multiple functions have been proposed, such a...

journal_title：Proteomics

authors： Banfi C,Brioschi M,Wait R,Begum S,Gianazza E,Fratto P,Polvani G,Vitali E,Parolari A,Mussoni L,Tremoli E

更新日期：2006-03-01 00:00:00

abstract：:Four rhoptry proteins (ROP) of Toxoplasma gondii previously identified with mAb have been affinity purified and analyzed by MS; the data obtained allowed the genomic sequences to be assigned to these proteins. As previously suggested for some of them by antibody crossreactivity, these proteins were shown to belong to ...

journal_title：Proteomics

authors： El Hajj H,Demey E,Poncet J,Lebrun M,Wu B,Galéotti N,Fourmaux MN,Mercereau-Puijalon O,Vial H,Labesse G,Dubremetz JF

更新日期：2006-11-01 00:00:00

abstract：:Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 microM Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification....

journal_title：Proteomics

authors： Kieffer P,Dommes J,Hoffmann L,Hausman JF,Renaut J

更新日期：2008-06-01 00:00:00

abstract：:Separation or prefractionation of proteins by gel electrophoresis, followed by in-gel proteolytic digest and analysis by MS is a typical standard application in proteomics. For many laboratories, it is not cost-effective to use a robot with all its drawbacks. On the other hand, manual digest is time consuming and not ...

journal_title：Proteomics

authors： Franz T,Li X

更新日期：2012-08-01 00:00:00

abstract：:Campylobacter jejuni is a major gastrointestinal pathogen that colonizes host mucosa via interactions with extracellular matrix proteins, such as fibronectin (Fn). Fn-binding is mediated by a 37 kDa outer membrane protein termed Campylobacter adherence Factor (CadF). The outer membrane protein profile of a recent gast...

journal_title：Proteomics

authors： Scott NE,Marzook NB,Deutscher A,Falconer L,Crossett B,Djordjevic SP,Cordwell SJ

更新日期：2010-01-01 00:00:00

abstract：:Adiponectin is a fat cell-secreted hormone with antidiabetic and anti-inflammatory activities. The reduced adiponectin levels are associated with obesity-related metabolic syndrome. Replenishment of this hormone into animal models can improve insulin sensitivity, decrease blood glucose and lipid levels, and prevent th...

journal_title：Proteomics

authors： Wang Y,Xu LY,Lam KS,Lu G,Cooper GJ,Xu A

更新日期：2006-07-01 00:00:00

abstract：:Sporozoites of the malaria parasite Plasmodium are transmitted by mosquitoes and infect the liver for an initial and obligatory round of replication, before exponential multiplication in the blood and onset of the disease. Sporozoites and liver stages provide attractive targets for malaria vaccines and prophylactic dr...

journal_title：Proteomics

authors： Hamada S,Pionneau C,Parizot C,Silvie O,Chardonnet S,Marinach C

更新日期：2021-01-16 00:00:00

abstract：:Identification and analysis of types of biological protein-protein interactions and their interfaces to predict obligate and non-obligate complexes is a problem that has drawn the attention of the research community in the past few years. In this paper, we propose a prediction approach to predict these two types of co...

journal_title：Proteomics

authors： Aziz MM,Maleki M,Rueda L,Raza M,Banerjee S

更新日期：2011-10-01 00:00:00

abstract：:Low levels and long term exposure to benzene is associated with hematotoxicity including aplastic anemia, acute myelogenous leukemia, and lymphoma. Current biomonitoring methods such as urinary phenol, S-phenylmercapturic acid, and trans-trans muconic acid were found to be unreliable as analytical methods to detect be...

journal_title：Proteomics

authors： Joo WA,Kang MJ,Son WK,Lee HJ,Lee DY,Lee E,Kim CW

更新日期：2003-12-01 00:00:00

abstract：:Since its discovery more than 50 years ago, post-translational modification (PTM) of proteins via methylation has grown in prominence, its involvement having been recognised in a number of central processes in the cell. Of these, the best characterised is its role in the epigenetic code. However, there is increasing e...

journal_title：Proteomics

authors： Erce MA,Pang CN,Hart-Smith G,Wilkins MR

更新日期：2012-02-01 00:00:00

abstract：:Antibody microarrays have great potential for significant value in biological research. Cancer research in particular could benefit from the unique experimental capabilities of this technology. This article examines the current state of antibody microarray technological developments and assay formats, along with a rev...

journal_title：Proteomics

authors： Haab BB

更新日期：2003-11-01 00:00:00

abstract：:A low virulent Candida albicans mutant, CNC13, deleted in the Mitogen Activated Protein (MAP) kynase HOG1 was used to immunize BALB/c mice. Hog1p is essential for the oxidative stress and hyperosmolarity responses. Several doses and immunization procedures were employed. The protection capacity of the different sera g...

journal_title：Proteomics

authors： Fernández-Arenas E,Molero G,Nombela C,Diez-Orejas R,Gil C

更新日期：2004-04-01 00:00:00

abstract：:In this study, various proteomics-based methods were utilized to examine the 14-3-3 protein family in Arabidopsis thaliana. A protein extract was prepared from an Arabidopsis hypocotyl suspension culture and analyzed by two-dimensional gel electrophoresis and immunoblotting with a 14-3-3 monoclonal antibody that recog...

journal_title：Proteomics

authors： Fuller B,Stevens SM Jr,Sehnke PC,Ferl RJ

更新日期：2006-05-01 00:00:00

abstract：:Myogenesis is a complex process of which the underlying mechanisms are conserved between species, including birds and mammals. Despite a good understanding of the stages of myogenesis, many of the mechanisms involved in the regulation of proliferation of the successive myoblast generations, the cellular transitions ce...

journal_title：Proteomics

authors： Chaze T,Meunier B,Chambon C,Jurie C,Picard B

更新日期：2008-10-01 00:00:00

abstract：:Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...

journal_title：Proteomics

authors： Chaker S,Kashat L,Voisin S,Kaur J,Kak I,MacMillan C,Ozcelik H,Siu KW,Ralhan R,Walfish PG

更新日期：2013-03-01 00:00:00

abstract：:We previously reported that NSC606985, a new camptothecin analog, induces apoptosis of acute myeloid leukemic cells, which is triggered by proteolytic activation of protein kinase C delta (PKC delta). Here, we performed quantitative proteomic analysis of NSC606985-treated and untreated leukemic U937 cells with two-dim...

journal_title：Proteomics

authors： Zheng Y,Wang LS,Xia L,Han YH,Liao SH,Wang XL,Cheng JK,Chen GQ

更新日期：2009-04-01 00:00:00

abstract：:Acute kidney injury (AKI) is one of the more frequent and lethal pathological conditions seen in intensive care units. Currently available treatments are not totally effective but stem cell-based therapies are emerging as promising alternatives, especially the use of mesenchymal stromal cells (MSC), although the signa...

journal_title：Proteomics

authors： da Costa MR,Pizzatti L,Lindoso RS,Sant'Anna JF,DuRocher B,Abdelhay E,Vieyra A

更新日期：2014-06-01 00:00:00

abstract：:Prevotella intermedia binds and invades a variety of host cells. This binding is most probably mediated through cell surface proteins termed adhesins. To identify proteins binding to the host extracellular matrix (ECM) component, fibronectin, and study the molecular mechanism underlying bacterial colonization, we appl...

journal_title：Proteomics

authors： Yu F,Iyer D,Anaya C,Lewis JP

更新日期：2006-11-01 00:00:00

abstract：:In this study we investigated the cellular response to the application of high hydrostatic pressure. High pressure is increasingly used for food preservation. With high resolution 2-D electrophoresis we compared the protein patterns of atmospherically grown Lactobacillus sanfranciscensis with those pressure treated up...

journal_title：Proteomics

authors： Drews O,Weiss W,Reil G,Parlar H,Wait R,Görg A

更新日期：2002-06-01 00:00:00

abstract：:Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized ...

journal_title：Proteomics

authors： Holzlechner M,Reitschmidt S,Gruber S,Zeilinger S,Marchetti-Deschmann M

更新日期：2016-06-01 00:00:00

abstract：:To implement the 2-DE database of serogroup A Neisseria meningitidis (MenA) and improve its potential of investigation in bacterial biology, cell extracts were separated by tricine-SDS-PAGE and 131 novel proteins were identified by microLC-ESI-IT-MS/MS. These identifications extended to 404, the number of MenA gene ex...

journal_title：Proteomics

authors： Bernardini G,Arena S,Braconi D,Scaloni A,Santucci A

更新日期：2007-09-01 00:00:00

abstract：:Infection with the human liver fluke, Opisthorchis viverrini, is a serious public health problem in Thailand, Laos and nearby locations in Southeast Asia. Both experimental and epidemiological evidence strongly implicate liver fluke infection in the etiology of one of the liver cancer subtypes, cholangiocarcinoma (CCA...

journal_title：Proteomics

authors： Mulvenna J,Sripa B,Brindley PJ,Gorman J,Jones MK,Colgrave ML,Jones A,Nawaratna S,Laha T,Suttiprapa S,Smout MJ,Loukas A

更新日期：2010-03-01 00:00:00

abstract：:The large interactive surfaces of nanoparticles (NPs) increase the opportunities to develop NPs for vascular targeting. Proteomic analysis of endothelial cells exposed to NPs reveals the cellular response and turns the focus into the impairment of the endothelial permeability. Here, quantitative proteomics and transcr...

journal_title：Proteomics

authors： Kuruvilla J,Bayat N,Cristobal S

更新日期：2019-03-01 00:00:00

abstract：:Normal Chinese Liver Proteome Expression Profile is one of the major parts of Human Liver Proteome Project. Before starting the studies, it is necessary to examine the interindividual variation of normal liver proteome and evaluate the minimal size of samples for proteomic analysis. In this study, normal liver samples...

journal_title：Proteomics

authors： Zhang X,Guo Y,Song Y,Sun W,Yu C,Zhao X,Wang H,Jiang H,Li Y,Qian X,Jiang Y,He F

更新日期：2006-10-01 00:00:00