Abstract:
:Separation or prefractionation of proteins by gel electrophoresis, followed by in-gel proteolytic digest and analysis by MS is a typical standard application in proteomics. For many laboratories, it is not cost-effective to use a robot with all its drawbacks. On the other hand, manual digest is time consuming and not free of error if many samples are processed at the same time. The OASIS® HLB μElution plate allows the handling of 96 samples for protein in-gel digest and peptide desalting in a semiautomatic way. Using multichannel pipettes, solutions are added quickly, and with the use of the positive pressure-96 stand all solutions are removed simultaneously. In this article, a complete and detailed protocol for the use of the OASIS® HLB μElution plate is introduced and its effectiveness is shown with 2D- and 1D-gel samples.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Franz T,Li Xdoi
10.1002/pmic.201100354subject
Has Abstractpub_date
2012-08-01 00:00:00pages
2487-92issue
15-16eissn
1615-9853issn
1615-9861journal_volume
12pub_type
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