Quantitative proteomics characterization on the antitumor effects of isodeoxyelephantopin against nasopharyngeal carcinoma.


:Isolated from Elephantopus scaber L., a Chinese medicinal herb that is widely used to prevent and treat cancers in China, isodeoxyelephantopin (ESI) exerted antitumor effects on several cancer cells. However, its antitumor mechanism is still not clear. In this study, we found that ESI could induce G2/M arrest and subsequently stimulate cell apoptosis in dose- and time-dependent manners. We used SILAC quantitative proteomics to identify ESI-regulated proteins in cancer cells, and found that 124 proteins were significantly altered in expression. Gene ontology and Ingenuity Pathway Analysis revealed that these proteins were mainly involved in the regulation of oxidative stress and inflammation response. Functional studies demonstrated that ESI induced G2/M arrest and apoptosis by inducing ROS generation, and that antioxidant N-acetyl-l-cysteine could block the ESI-induced antitumor effects. Accumulated ROS resulted in DNA breakage, subsequent G2/M arrest and mitochondrial-mediated apoptosis. ESI upregulated the expression of anticancer inflammation factors IL-12a, IFN-α, and IFN-β through ROS-dependent and independent pathways. The current work reveals that ESI exerts its antitumor effects through ROS-dependent DNA damage, mitochondrial-mediated apoptosis mechanism and antitumor inflammation factor pathway.






Yan GR,Tan Z,Wang Y,Xu ML,Yu G,Li Y,He QY




Has Abstract


2013-11-01 00:00:00












  • Identification of the degradome of Isp-1, a major intracellular serine protease of Bacillus subtilis, by two-dimensional gel electrophoresis and matrix- assisted laser desorption/ionization-time of flight analysis.

    abstract::Intracellular serine protease-1 (Isp-1) is a major intracellular serine protease of Bacillus subtilis, whose functions still remain largely unknown. Furthermore, physiological substrates are yet to be determined. To identify Isp-1 substrates, we digested extract obtained from an Isp-1 deficient Bacillus mutant with pu...


    pub_type: 杂志文章


    authors: Lee AY,Goo Park S,Kho CW,Young Park S,Cho S,Lee SC,Lee DH,Myung PK,Park BC

    更新日期:2004-11-01 00:00:00

  • Characterization of microsomal fraction proteome in human lymphoblasts reveals the down-regulation of galectin-1 by interleukin-12.

    abstract::T helper cells (Th) are divided into Th1 and Th2 subsets based upon their cytokine profiles and function. Naïve Th cells differentiate into Th1 and Th2 subsets depending on the antigens, costimulatory molecules, and cytokines they encounter. Cytokine interleukin (IL)-12 enhances the generation of Th1 lymphocytes and i...


    pub_type: 杂志文章


    authors: Filén JJ,Nyman TA,Korhonen J,Goodlett DR,Lahesmaa R

    更新日期:2005-12-01 00:00:00

  • Numerical approaches for quantitative analysis of two-dimensional maps: a review of commercial software and home-made systems.

    abstract::The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...


    pub_type: 杂志文章,评审


    authors: Marengo E,Robotti E,Antonucci F,Cecconi D,Campostrini N,Righetti PG

    更新日期:2005-02-01 00:00:00

  • Proteasome subunit proteins and neuropathology in tauopathies and synucleinopathies: Consequences for proteomic analyses.

    abstract::Accumulation of proteins in inclusions in neurological disorders is partly due to dysfunction of the ubiquitin-proteasome system. Proteasomal dysfunction may be caused by misexpression of one or more of its subunits. A large number of antibodies reactive with proteasome subunits were screened on material from patients...


    pub_type: 杂志文章


    authors: Zouambia M,Fischer DF,Hobo B,De Vos RA,Hol EM,Varndell IM,Sheppard PW,Van Leeuwen FW

    更新日期:2008-03-01 00:00:00

  • GlycoMod--a software tool for determining glycosylation compositions from mass spectrometric data.

    abstract::GlycoMod (http://www.expasy.ch/tools/glycomod/) is a software tool designed to find all possible compositions of a glycan structure from its experimentally determined mass. The program can be used to predict the composition of any glycoprotein-derived oligosaccharide comprised of either underivatised, methylated or ac...


    pub_type: 杂志文章


    authors: Cooper CA,Gasteiger E,Packer NH

    更新日期:2001-02-01 00:00:00

  • Two-dimensional separation of human plasma proteins using iterative free-flow electrophoresis.

    abstract::Blood plasma is the most complex human-derived proteome, containing other tissue proteomes as subsets. This proteome has only been partially characterized due to the extremely wide dynamic range of the plasma proteins of more than ten orders of magnitude. Thus, the reduction in sample complexity prior to mass spectrom...


    pub_type: 杂志文章


    authors: Nissum M,Kuhfuss S,Hauptmann M,Obermaier C,Sukop U,Wildgruber R,Weber G,Eckerskorn C,Malmström J

    更新日期:2007-12-01 00:00:00

  • The use of ubiquitin lysine mutants to characterize E2-E3 linkage specificity: Mass spectrometry offers a cautionary "tail".

    abstract::Oligomeric ubiquitin structures (i.e. ubiquitin "chains") may be formed through any of seven different lysine residues in the polypeptide, or via the amine group of Met 1. Different types of ubiquitin chains can confer very different biological outcomes to a protein substrate, yet the structural characteristics of E2s...


    pub_type: 杂志文章


    authors: Hong JH,Ng D,Srikumar T,Raught B

    更新日期:2015-09-01 00:00:00

  • Rapid protein identification using monolithic enzymatic microreactor and LC-ESI-MS/MS.

    abstract::A monolithic enzymatic microreactor was prepared in a fused-silica capillary by in situ polymerization of acrylamide, glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) in the presence of a binary porogenic mixture of dodecanol and cyclohexanol, followed by ammonia solution treatment, glutaraldehyde activa...


    pub_type: 杂志文章


    authors: Duan J,Liang Z,Yang C,Zhang J,Zhang L,Zhang W,Zhang Y

    更新日期:2006-01-01 00:00:00

  • Extracellular Ser/Thr/Tyr phosphorylated proteins of Pseudomonas aeruginosa PA14 strain.

    abstract::Protein phosphorylation on serine, threonine, and tyrosine is known to be involved in a wide variety of cellular processes, signal transduction, and bacterial virulence. We characterized, for the first time, the extracellular phosphoproteins of the Pseudomonas aeruginosa PA14 strain. We identified 28 phosphoproteins (...


    pub_type: 杂志文章


    authors: Ouidir T,Jarnier F,Cosette P,Jouenne T,Hardouin J

    更新日期:2014-09-01 00:00:00

  • Analysis of earthworm Eisenia fetida proteomes during cadmium exposure: An ecotoxicoproteomics approach.

    abstract::Identification of differential proteomic responses to cadmium (Cd) would provide a means for better understanding the survival mechanisms of the earthworm, Eisenia fetida, living in a Cd-polluted environment, and the stress responses can be assessed by ecotoxiptoromics approaches. Extracts of whole earthworm collected...


    pub_type: 杂志文章


    authors: Wang X,Chang L,Sun Z,Zhang Y,Yao L

    更新日期:2010-12-01 00:00:00

  • Characterization of the human urine proteome by preparative electrophoresis in combination with 2-DE.

    abstract::The protein components of urine are useful indicators of renal function and human health in general. Urine samples are easily attainable making them ideal substrates for biomarker research. Analysis of the urine proteome however, has been hindered by the great variability of the urine specimens, and the presence of va...


    pub_type: 杂志文章


    authors: Zerefos PG,Vougas K,Dimitraki P,Kossida S,Petrolekas A,Stravodimos K,Giannopoulos A,Fountoulakis M,Vlahou A

    更新日期:2006-08-01 00:00:00

  • Virulence of Erwinia amylovora, a prevalent apple pathogen: Outer membrane proteins and type III secreted effectors increase fitness and compromise plant defenses.

    abstract::Until now, no data are available on the outer membrane (OM) proteome of Erwinia amylovora, a Gram-negative plant pathogen, causing fire blight in most of the members of the Rosaceae family. Since the OM forms the interface between the bacterial cell and its environment it is in direct contact with the host. Additional...


    pub_type: 杂志文章


    authors: Holtappels M,Noben JP,Valcke R

    更新日期:2016-09-01 00:00:00

  • Proteomic identification of mammalian cell surface derived glycosylphosphatidylinositol-anchored proteins through selective glycan enrichment.

    abstract::Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are an important class of glycoproteins that are tethered to the surface of mammalian cells via the lipid GPI. GPI-APs have been implicated in many important cellular functions including cell adhesion, cell signaling, and immune regulation. Proteomic identificat...


    pub_type: 杂志文章


    authors: Cortes LK,Vainauskas S,Dai N,McClung CM,Shah M,Benner JS,Corrêa IR Jr,VerBerkmoes NC,Taron CH

    更新日期:2014-11-01 00:00:00

  • Serum biomarker discovery in renal cancer using 2-DE and prefractionation by immunodepletion and isoelectric focusing; increasing coverage or more of the same?

    abstract::As an initial screen for novel markers of renal cancer and to minimise background heterogeneity, we have compared the within-patient profiles of serum samples from seven patients pre- and post-nephrectomy. Samples were depleted of six of the most abundant proteins using Agilent's multiple affinity removal system (MARS...


    pub_type: 杂志文章


    authors: Vasudev NS,Ferguson RE,Cairns DA,Stanley AJ,Selby PJ,Banks RE

    更新日期:2008-12-01 00:00:00

  • Partial hepatectomy induced liver proteome changes in mice.

    abstract::Acceleration of liver regeneration could be of great clinical benefit in various liver-associated diseases. However, at present little is known about therapeutic interventions to enhance this regenerative process. Our limited understanding and the complexity of the mechanisms involved have prevented the identification...


    pub_type: 杂志文章


    authors: Strey CW,Winters MS,Markiewski MM,Lambris JD

    更新日期:2005-01-01 00:00:00

  • Albumin depletion of human plasma also removes low abundance proteins including the cytokines.

    abstract::The use of proteomics for efficient, accurate, and complete analysis of clinical samples poses a variety of technical challenges. The presence of higher abundance proteins in the plasma, such as albumin, may mask the detection of lower abundance proteins such as the cytokines. Methods have been proposed to deplete the...


    pub_type: 杂志文章


    authors: Granger J,Siddiqui J,Copeland S,Remick D

    更新日期:2005-12-01 00:00:00

  • Proteomics of hydrophobic samples: Fast, robust and low-cost workflows for clinical approaches.

    abstract::In a comparative study, we investigated the influence of nine sample preparation workflows and seven different lysis buffers for qualitative and quantitative analysis of the human adipose tissue proteome. Adipose tissue is not just a fat depot but also an endocrine organ, which cross-talks with other tissue types and ...


    pub_type: 杂志文章


    authors: Pasing Y,Colnoe S,Hansen T

    更新日期:2017-03-01 00:00:00

  • Placenta Stem/Stromal Cell-Derived Extracellular Vesicles for Potential Use in Lung Repair.

    abstract::Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...


    pub_type: 杂志文章


    authors: Kim SY,Joglekar MV,Hardikar AA,Phan TH,Khanal D,Tharkar P,Limantoro C,Johnson J,Kalionis B,Chrzanowski W

    更新日期:2019-09-01 00:00:00

  • NanoLC-FT-ICR MS improves proteome coverage attainable for approximately 3000 laser-microdissected breast carcinoma cells.

    abstract::Proteomics assays hold great promise for unraveling molecular events that underlie human diseases. Effective analysis of clinical samples is essential, but this task is considerably complicated by tissue heterogeneity. Laser capture microdissection (LCM) can be used to selectively isolate target cells from their nativ...


    pub_type: 杂志文章


    authors: Umar A,Luider TM,Foekens JA,Pasa-Tolić L

    更新日期:2007-01-01 00:00:00

  • Predicting adverse drug reaction profiles by integrating protein interaction networks with drug structures.

    abstract::The prediction of adverse drug reactions (ADRs) has become increasingly important, due to the rising concern on serious ADRs that can cause drugs to fail to reach or stay in the market. We proposed a framework for predicting ADR profiles by integrating protein-protein interaction (PPI) networks with drug structures. W...


    pub_type: 杂志文章


    authors: Huang LC,Wu X,Chen JY

    更新日期:2013-01-01 00:00:00

  • A detergent- and cyanogen bromide-free method for integral membrane proteomics: application to Halobacterium purple membranes and the human epidermal membrane proteome.

    abstract::A simple and rapid method for characterizing hydrophobic integral membrane proteins and its utility for membrane proteomics using microcapillary liquid chromatography coupled on-line with tandem mass spectrometry (microLC-MS/MS) is described. The present technique does not rely on the use of detergents, strong organic...


    pub_type: 杂志文章


    authors: Blonder J,Conrads TP,Yu LR,Terunuma A,Janini GM,Issaq HJ,Vogel JC,Veenstra TD

    更新日期:2004-01-01 00:00:00

  • Use of two-dimensional gel electrophoresis proteome reference maps of dinoflagellates for species recognition of causative agents of harmful algal blooms.

    abstract::The sample preparation procedures established for Prorocentrum triestinum were adapted to cover both thecate and athecate dinoflagellates. Further, whether trichloroacetic acid (TCA) precipitation can be used to fix and preserve the harmful or nuisance species from local waters that they infest was tested. Optimized t...


    pub_type: 杂志文章


    authors: Chan LL,Hodgkiss IJ,Lu S,Lo SC

    更新日期:2004-01-01 00:00:00

  • An efficient solubilization buffer for plant proteins focused in immobilized pH gradients.

    abstract::The solubilization of a large array of proteins before electrophoresis itself is a very critical point for proteomic analyses. We compared the efficiency of several different solubilization buffers. From this work, we defined a very efficient solubilization buffer, including two chaotropes, two reducing agents (R2), t...


    pub_type: 杂志文章


    authors: Méchin V,Consoli L,Le Guilloux M,Damerval C

    更新日期:2003-07-01 00:00:00

  • Proteomic trajectory mapping of biological transformation: Application to developmental mouse retina.

    abstract::In this report we introduce a new concept "proteomic trajectory mapping" for the investigation of a complex phenomenon underlying biological transformation and transition. We define proteomic trajectory to be the kinetic trace of protein expression and present a successful proteomic trajectory mapping of complex molec...


    pub_type: 杂志文章


    authors: Haniu H,Komori N,Takemori N,Singh A,Ash JD,Matsumoto H

    更新日期:2006-06-01 00:00:00

  • Proteomic Analysis of Endothelial Cells Exposed to Ultrasmall Nanoparticles Reveals Disruption in Paracellular and Transcellular Transport.

    abstract::The large interactive surfaces of nanoparticles (NPs) increase the opportunities to develop NPs for vascular targeting. Proteomic analysis of endothelial cells exposed to NPs reveals the cellular response and turns the focus into the impairment of the endothelial permeability. Here, quantitative proteomics and transcr...


    pub_type: 杂志文章


    authors: Kuruvilla J,Bayat N,Cristobal S

    更新日期:2019-03-01 00:00:00

  • A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli.

    abstract::In prokaryotes, acyl carrier protein (ACP) is a cofactor central to a myriad of syntheses, including fatty acid and phospholipid synthesis. To fulfill its function, ACP must therefore interact with a multitude of different enzymes, which includes the thioesterase YbgC. We found a specific interaction between ACP and Y...


    pub_type: 杂志文章


    authors: Gully D,Bouveret E

    更新日期:2006-01-01 00:00:00

  • Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma.

    abstract::Systematic detection of low-abundance proteins in human blood that may be putative disease biomarkers is complicated by an extremely wide range of protein abundances. Hence, depletion of major proteins is one potential strategy for enhancing detection sensitivity in serum or plasma. This study compared a recently comm...


    pub_type: 杂志文章


    authors: Echan LA,Tang HY,Ali-Khan N,Lee K,Speicher DW

    更新日期:2005-08-01 00:00:00

  • RADARS, a bioinformatics solution that automates proteome mass spectral analysis, optimises protein identification, and archives data in a relational database.

    abstract::RADARS, a rapid, automated, data archiving and retrieval software system for high-throughput proteomic mass spectral data processing and storage, is described. The majority of mass spectrometer data files are compatible with RADARS, for consistent processing. The system automatically takes unprocessed data files, iden...


    pub_type: 杂志文章


    authors: Field HI,Fenyö D,Beavis RC

    更新日期:2002-01-01 00:00:00

  • The Equine PeptideAtlas: a resource for developing proteomics-based veterinary research.

    abstract::Progress in MS-based methods for veterinary research and diagnostics is lagging behind compared to the human research, and proteome data of domestic animals is still not well represented in open source data repositories. This is particularly true for the equine species. Here we present a first Equine PeptideAtlas enco...


    pub_type: 杂志文章


    authors: Bundgaard L,Jacobsen S,Sørensen MA,Sun Z,Deutsch EW,Moritz RL,Bendixen E

    更新日期:2014-03-01 00:00:00

  • Quantitative Proteomic Analysis of Staphylococcus aureus Treated With Punicalagin, a Natural Antibiotic From Pomegranate That Disrupts Iron Homeostasis and Induces SOS.

    abstract::Staphylococcus aureus, a bacterial, food-borne pathogen of humans, can contaminate raw fruits and vegetables. While physical and chemical methods are available to control S. aureus, scientists are searching for inhibitory phytochemicals from plants. One promising compound from pomegranate is punicalagin, a natural ant...


    pub_type: 杂志文章


    authors: Cooper B,Islam N,Xu Y,Beard HS,Garrett WM,Gu G,Nou X

    更新日期:2018-05-01 00:00:00