Quantitative proteomics characterization on the antitumor effects of isodeoxyelephantopin against nasopharyngeal carcinoma.

Abstract:

:Isolated from Elephantopus scaber L., a Chinese medicinal herb that is widely used to prevent and treat cancers in China, isodeoxyelephantopin (ESI) exerted antitumor effects on several cancer cells. However, its antitumor mechanism is still not clear. In this study, we found that ESI could induce G2/M arrest and subsequently stimulate cell apoptosis in dose- and time-dependent manners. We used SILAC quantitative proteomics to identify ESI-regulated proteins in cancer cells, and found that 124 proteins were significantly altered in expression. Gene ontology and Ingenuity Pathway Analysis revealed that these proteins were mainly involved in the regulation of oxidative stress and inflammation response. Functional studies demonstrated that ESI induced G2/M arrest and apoptosis by inducing ROS generation, and that antioxidant N-acetyl-l-cysteine could block the ESI-induced antitumor effects. Accumulated ROS resulted in DNA breakage, subsequent G2/M arrest and mitochondrial-mediated apoptosis. ESI upregulated the expression of anticancer inflammation factors IL-12a, IFN-α, and IFN-β through ROS-dependent and independent pathways. The current work reveals that ESI exerts its antitumor effects through ROS-dependent DNA damage, mitochondrial-mediated apoptosis mechanism and antitumor inflammation factor pathway.

journal_name

Proteomics

journal_title

Proteomics

authors

Yan GR,Tan Z,Wang Y,Xu ML,Yu G,Li Y,He QY

doi

10.1002/pmic.201300152

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

3222-32

issue

21

eissn

1615-9853

issn

1615-9861

journal_volume

13

pub_type

杂志文章
  • In Depth Quantitative Proteomic and Transcriptomic Characterization of Human Adipocyte Differentiation Using the SGBS Cell Line.

    abstract::Most information on molecular processes accompanying and driving adipocyte differentiation are derived from rodent models. Here, we provide a comprehensive analysis of combined transcriptomic and proteomic alterations during adipocyte differentiation in Simpson-Golabi-Behmel Syndrome (SGBS) cells. The SGBS cells are a...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201900405

    authors: Kalkhof S,Krieg L,Büttner P,Wabitsch M,Küntzel C,Friebe D,Landgraf K,Hanschkow M,Schubert K,Kiess W,Krohn K,Blüher M,von Bergen M,Körner A

    更新日期:2020-05-08 00:00:00

  • A rapid and simple procedure for the depletion of abundant storage proteins from legume seeds to advance proteome analysis: a case study using Glycine max.

    abstract::2-D analysis of plant proteomes containing thousands of proteins has limited dynamic resolution because only abundant proteins can be detected. Proteomic assessment of the non-abundant proteins within seeds is difficult when 60-80% is storage proteins. Resolution can be improved through sample fractionation using sepa...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200800875

    authors: Krishnan HB,Oehrle NW,Natarajan SS

    更新日期:2009-06-01 00:00:00

  • Analysis of the interaction of influenza virus polymerase complex with human cell factors.

    abstract::The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. Here we present the characterisation of the complexes formed intracellularly by the influenza polymerase in human cells. The virus polymerase was expressed ...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200700508

    authors: Jorba N,Juarez S,Torreira E,Gastaminza P,Zamarreño N,Albar JP,Ortín J

    更新日期:2008-05-01 00:00:00

  • N-Terminal methylation of proteasome subunit Rpt1 in yeast.

    abstract::The 26S proteasome is a multicatalytic protease complex that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core (the 20S proteasome) as well as regulatory particles, which contain six ATPase (Rpt) subunits involved in unfolding and translocation of substrates to the catalytic chambe...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201300207

    authors: Kimura Y,Kurata Y,Ishikawa A,Okayama A,Kamita M,Hirano H

    更新日期:2013-11-01 00:00:00

  • Renal cell carcinoma: handling and treatment.

    abstract::The quality of samples and of pre-analytical steps are crucial in all biological tests, this is dramatically true in proteomics analysis. In renal cell carcinoma preparation for two-dimensional gel electrophoresis the time elapsed between sample collection and treatment, and the heterogeneity of tissues are considered...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/1615-9861(200211)2:11<1627::AID-PROT1627>3

    authors: Sarto C,Valsecchi C,Mocarelli P

    更新日期:2002-11-01 00:00:00

  • Comparative proteomic analysis of human pancreatic juice: methodological study.

    abstract::Pancreatic cancer is the most lethal of all the common malignancies. Markers for early detection of this disease are urgently needed. Here, we optimized and applied a proteome analysis of human pancreatic juice to identify biomarkers for pancreatic cancer. Pancreatic juice samples, devoid of blood or bile contaminatio...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200600086

    authors: Zhou L,Lu Z,Yang A,Deng R,Mai C,Sang X,Faber KN,Lu X

    更新日期:2007-04-01 00:00:00

  • Proteomic analysis in human breast cancer: identification of a characteristic protein expression profile of malignant breast epithelium.

    abstract::Gene expression analysis has become a promising tool in predicting the clinical course of malignant disease and the response to antineoplastic therapy. Surprisingly, only little is known about the protein expression pattern of human tumors. Recent advances in proteomic analysis allow proteins of interest to be identif...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200500129

    authors: Hudelist G,Singer CF,Pischinger KI,Kaserer K,Manavi M,Kubista E,Czerwenka KF

    更新日期:2006-03-01 00:00:00

  • Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network.

    abstract::Proteolysis affects every protein at some point in its life cycle. Many biomarkers of disease or cancer are stable proteolytic fragments in biological fluids. There is great interest and a challenge in proteolytically modified protein study to identify physiologic protease-substrate relationships and find potential bi...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201200027

    authors: Shen C,Yu Y,Li H,Yan G,Liu M,Shen H,Yang P

    更新日期:2012-06-01 00:00:00

  • New mortalin and histidyl tRNA synthetase isoforms point out a pitfall in proteomic analysis of Egr1 genetically modified mice.

    abstract::Egr1 (Zif268) is an immediate early gene encoding an inducible transcription factor involved in synaptic plasticity and several forms of memory in rodents. Using 2-DE and MS, we compared proteomes of hippocampal subregions and cortex in Egr1-deficient and wild-type littermates. Two significant differences were identif...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200600513

    authors: Chardonnet S,Decottignies P,Amar L,Le Caer JP,Davis S,Laroche S,Le Maréchal P

    更新日期:2007-01-01 00:00:00

  • Subproteomes of soluble and structure-bound Helicobacter pylori proteins analyzed by two-dimensional gel electrophoresis and mass spectrometry.

    abstract::Helicobacter pylori is one of the most common bacterial pathogens and causes a variety of diseases, such as peptic ulcer or gastric cancer. Despite intensive study of this human pathogen in the last decades, knowledge about its membrane proteins and, in particular, those which are putative components of the type IV se...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200401019

    authors: Backert S,Kwok T,Schmid M,Selbach M,Moese S,Peek RM Jr,König W,Meyer TF,Jungblut PR

    更新日期:2005-04-01 00:00:00

  • Expanding the mouse embryonic stem cell proteome: combining three proteomic approaches.

    abstract::The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic dig...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201000039

    authors: Gundry RL,Tchernyshyov I,Sheng S,Tarasova Y,Raginski K,Boheler KR,Van Eyk JE

    更新日期:2010-07-01 00:00:00

  • Ligand-independent orphan receptor TR2 activation by phosphorylation at the DNA-binding domain.

    abstract::In a previous report we demonstrated protein kinase C (PKC)-mediated phosphorylation of the ligand-binding domain (LBD) of orphan nuclear receptor TR2. In this report, we provide the evidence of PKC-mediated phosphorylation of the DNA-binding domain (DBD) of TR2. Two PKC target sites were predicted within the DBD, at ...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200500068

    authors: Khan SA,Park SW,Huq MD,Wei LN

    更新日期:2006-01-01 00:00:00

  • Comprehensive Peptide Analysis of Mouse Brain Striatum Identifies Novel sORF-Encoded Polypeptides.

    abstract::Bio-active peptides are involved in the regulation of most physiological processes in the body. Classical bio-active peptides (CBAPs) are cleaved from a larger precursor protein and stored in secretion vesicles from which they are released in the extracellular space. Recently, another non-classical type of bio-active ...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201700218

    authors: Budamgunta H,Olexiouk V,Luyten W,Schildermans K,Maes E,Boonen K,Menschaert G,Baggerman G

    更新日期:2018-05-01 00:00:00

  • Phosphorylation of the SSBP2 and ABL proteins by the ZNF198-FGFR1 fusion kinase seen in atypical myeloproliferative disorders as revealed by phosphopeptide-specific MS.

    abstract::The ZNF198-fibroblast growth factor receptor-1 (FGFR1) fusion kinase is a constitutively activated tyrosine kinase associated with a specific atypical myeloproliferative disease. The chimeric protein localizes to the cytoplasm, unlike the wild type FGFR1 receptor kinase, and presumably inappropriately phosphorylates s...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200800852

    authors: Kasyapa C,Gu TL,Nagarajan L,Polakiewicz R,Cowell JK

    更新日期:2009-08-01 00:00:00

  • Secretome proteins as candidate biomarkers for aggressive thyroid carcinomas.

    abstract::Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201200356

    authors: Chaker S,Kashat L,Voisin S,Kaur J,Kak I,MacMillan C,Ozcelik H,Siu KW,Ralhan R,Walfish PG

    更新日期:2013-03-01 00:00:00

  • An interaction proteomics survey of transcription factor binding at recurrent TERT promoter mutations.

    abstract::Aberrant telomerase reactivation in differentiated cells represents a major event in oncogenic transformation. Recurrent somatic mutations in the human telomerase reverse transcriptase (TERT) promoter region, predominantly localized to two nucleotide positions, are highly prevalent in many cancer types. Both mutations...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201500327

    authors: Makowski MM,Willems E,Fang J,Choi J,Zhang T,Jansen PW,Brown KM,Vermeulen M

    更新日期:2016-02-01 00:00:00

  • Proteomic analysis of primary esophageal squamous cell carcinoma reveals downregulation of a cell adhesion protein, periplakin.

    abstract::Recent advances in two-dimensional electrophoresis (2-DE) such as fluorescent 2-D differential gel electrophoresis (2-D DIGE) has made it possible to detect and quantitate the critical changes involved in disease pathogenesis. We have previously identified novel proteins with altered expression in primary colorectal c...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200500262

    authors: Nishimori T,Tomonaga T,Matsushita K,Oh-Ishi M,Kodera Y,Maeda T,Nomura F,Matsubara H,Shimada H,Ochiai T

    更新日期:2006-02-01 00:00:00

  • Ubiquitin specific peptidase 5 mediates Histidine-rich protein Hpn induced cell apoptosis in hepatocellular carcinoma through P14-P53 signaling.

    abstract::Hpn is a small histidine-rich cytoplasmic protein from Helicobacter pylori and has been recognized as a high-risk factor for several cancers including gastric cancer, colorectal cancer, and MALT lymphoma. However, the relationship between Hpn and cancers remains elusive. In this study, we discovered that Hpn protein e...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201600350

    authors: Liu Y,Wang WM,Zou LY,Li L,Feng L,Pan MZ,Lv MY,Cao Y,Wang H,Kung HF,Pang JX,Fu WM,Zhang JF

    更新日期:2017-06-01 00:00:00

  • Proteomics of aluminum tolerance in plants.

    abstract::Aluminum (Al) toxicity is a major constraint for plant root development and growth as well as crop yield in acidic soils, which constitute approximately 40% of the potentially arable lands worldwide. The mechanisms of Al tolerance in plants are not well understood. As a whole systems approach, proteomic techniques hav...

    journal_title:Proteomics

    pub_type: 杂志文章,评审

    doi:10.1002/pmic.201300252

    authors: Zheng L,Lan P,Shen RF,Li WF

    更新日期:2014-03-01 00:00:00

  • High-sensitivity HLA class I peptidome analysis enables a precise definition of peptide motifs and the identification of peptides from cell lines and patients' sera.

    abstract::The characterization of peptides bound to human leukocyte antigen (HLA) class I is of fundamental importance for understanding CD8+ T cell-driven immunological processes and for the development of immunomodulatory therapeutic strategies. However, until now, the mass spectrometric analysis of HLA-bound peptides has typ...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201500445

    authors: Ritz D,Gloger A,Weide B,Garbe C,Neri D,Fugmann T

    更新日期:2016-05-01 00:00:00

  • Stage-dependent increase of orosomucoid and zinc-alpha2-glycoprotein in urinary bladder cancer.

    abstract::Identification and characterization of biomarkers in body fluids such as serum or urine serve as a basis for early detection of diseases, particularly of cancer. Performing 2-DE with subsequent MS analyses, conventional immunoblotting and immunohistochemistry we identified two proteins, orosomucoid (ORM) and human zin...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200402005

    authors: Irmak S,Tilki D,Heukeshoven J,Oliveira-Ferrer L,Friedrich M,Huland H,Ergün S

    更新日期:2005-11-01 00:00:00

  • Identification of caspase-3 degradome by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight analysis.

    abstract::The activation of caspases is a critical event for the execution phase of programmed cell death. Caspases are highly specific in their ability to activate or inhibit many crucial proteins in the cell via site-specific cleavage. To date, more than 60 proteins have been shown to be substrates of one or more caspases in ...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200400979

    authors: Lee AY,Park BC,Jang M,Cho S,Lee DH,Lee SC,Myung PK,Park SG

    更新日期:2004-11-01 00:00:00

  • Comparative proteomic analysis of extracellular matrix proteins secreted by two types of skin fibroblasts.

    abstract::The hair follicle dermal papilla is composed primarily of extracellular matrix (ECM) proteins secreted by resident fibroblasts. Dermal papilla is endowed with hair morphogenic properties, yet its composition is poorly characterized. In an attempt to understand its specificity better, we compared the protein compositio...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200402040

    authors: Pflieger D,Chabane S,Gaillard O,Bernard BA,Ducoroy P,Rossier J,Vinh J

    更新日期:2006-11-01 00:00:00

  • Plant phosphoproteomics: a long road ahead.

    abstract::Phosphoproteomics can be defined as the comprehensive study of protein phosphorylation by identification of the phosphoproteins, exact mapping of the phosphorylation sites, quantification of phosphorylation, and eventually, revealing their biological function. Its place in today's research is vitally important to addr...

    journal_title:Proteomics

    pub_type: 杂志文章,评审

    doi:10.1002/pmic.200600232

    authors: Kersten B,Agrawal GK,Iwahashi H,Rakwal R

    更新日期:2006-10-01 00:00:00

  • Human colostrum: identification of minor proteins in the aqueous phase by proteomics.

    abstract::Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha-lactalbumin and HSA by immunoabsorption, using 2-...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200500558

    authors: Palmer DJ,Kelly VC,Smit AM,Kuy S,Knight CG,Cooper GJ

    更新日期:2006-04-01 00:00:00

  • Protein sequence tags: a novel solution for comparative proteomics.

    abstract::Comparative proteome profiling using stable isotope peptide labelling and mass spectrometry has emerged as a promising strategy. Here, we show the broad potential of our proprietary protein sequence tag (PST) technology. A special feature of PST is its ability to detect a wide variety of proteins including the pharmac...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200401306

    authors: Kuhn K,Prinz T,Schäfer J,Baumann C,Schärfke M,Kienle S,Schwarz J,Steiner S,Hamon C

    更新日期:2005-06-01 00:00:00

  • Protein profiling of epidermal bladder cells from the halophyte Mesembryanthemum crystallinum.

    abstract::Plant epidermal trichomes are as varied in morphology as they are in function. In the halophyte Mesembryanthemum crystallinum, specialized trichomes called epidermal bladder cells (EBC) line the surface of leaves and stems, and increase dramatically in size and volume upon plant salt-treatment. These cells have been p...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201200152

    authors: Barkla BJ,Vera-Estrella R,Pantoja O

    更新日期:2012-09-01 00:00:00

  • Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis.

    abstract::The Ser/Thr/Tyr phosphoproteome of Bacillus subtilis was analyzed by a 2-D gel-based approach combining Pro-Q Diamond staining and [(33)P]-labeling. In exponentially growing B. subtilis cells 27 proteins could be identified after staining with Pro-Q Diamond and/or [(33)P]-labeling and one additional protein was labele...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.200700232

    authors: Eymann C,Becher D,Bernhardt J,Gronau K,Klutzny A,Hecker M

    更新日期:2007-10-01 00:00:00

  • Proteomic profiling of extracellular vesicles secreted from Toxoplasma gondii.

    abstract::Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201600477

    authors: Wowk PF,Zardo ML,Miot HT,Goldenberg S,Carvalho PC,Mörking PA

    更新日期:2017-08-01 00:00:00

  • pseudoQC: A Regression-Based Simulation Software for Correction and Normalization of Complex Metabolomics and Proteomics Datasets.

    abstract::Various types of unwanted and uncontrollable signal variations in MS-based metabolomics and proteomics datasets severely disturb the accuracies of metabolite and protein profiling. Therefore, pooled quality control (QC) samples are often employed in quality management processes, which are indispensable to the success ...

    journal_title:Proteomics

    pub_type: 杂志文章

    doi:10.1002/pmic.201900264

    authors: Wang S,Yang H

    更新日期:2019-10-01 00:00:00