Abstract:
:The quantitative analysis of signaling networks requires highly sensitive methods for the time-resolved determination of protein phosphorylation. For this reason, we developed a quantitative protein microarray that monitors the activation of multiple signaling pathways in parallel, and at high temporal resolution. A label-free sandwich approach was combined with near infrared detection, thus permitting the accurate quantification of low-level phosphoproteins in limited biological samples corresponding to less than 50,000 cells, and with a very low standard deviation of approximately 5%. The identification of suitable antibody pairs was facilitated by determining their accuracy and dynamic range using our customized software package Quantpro. Thus, we are providing an important tool to generate quantitative data for systems biology approaches, and to drive innovative diagnostic applications.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Korf U,Derdak S,Tresch A,Henjes F,Schumacher S,Schmidt C,Hahn B,Lehmann WD,Poustka A,Beissbarth T,Klingmüller Udoi
10.1002/pmic.200800112subject
Has Abstractpub_date
2008-11-01 00:00:00pages
4603-12issue
21eissn
1615-9853issn
1615-9861journal_volume
8pub_type
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