A rare and often unrecognized cerebromeningitis and hemodynamic disorder: a major cause of sudden death in somatic cell cloned piglets.

abstract：:We describe a new approach for the characterization of a digested protein complex with quantitative aspects. Accurate masses of tryptic peptides in the digested complex were acquired by nano-liquid chromatography Fourier transform-ion cyclotron resonance mass spectrometry (MS). The conditions of the electrospray ion s...

journal_title：Proteomics

authors： Nakamura T,Dohmae N,Takio K

更新日期：2004-09-01 00:00:00

abstract：:With advances in liquid chromatography coupled to tandem mass spectrometry technologies combined with the continued goals of biomarker discovery, clinical applications of established biomarkers, and integrating large multiomic datasets (i.e. "big data"), there remains an urgent need for robust tools to assess instrume...

journal_title：Proteomics

authors： Bereman MS

更新日期：2015-03-01 00:00:00

abstract：:To develop a standard method for separating highly basic proteins in mammalian cells, we established a 2-D LC separation system coupled with chromatofocusing/nonporous RP column chromatography (CF/NPRPC) in a ProteomeLab PF2D system. After standardizing conditions for 2-D LC, a 2-D liquid protein map of uninfected mac...

journal_title：Proteomics

authors： Shin YK,Lee HJ,Lee JS,Paik YK

更新日期：2006-02-01 00:00:00

abstract：:Myelination of the CNS is performed by oligodendrocytes (OLs), which have been implicated in brain disorders, such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an OL reference proteome database. Proteins were prefractionationated by SDS-PAGE and after i...

journal_title：Proteomics

authors： Iwata K,Café-Mendes CC,Schmitt A,Steiner J,Manabe T,Matsuzaki H,Falkai P,Turck CW,Martins-de-Souza D

更新日期：2013-12-01 00:00:00

abstract：:The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better unders...

journal_title：Proteomics

authors： Victor B,Kanobana K,Gabriël S,Polman K,Deckers N,Dorny P,Deelder AM,Palmblad M

更新日期：2012-06-01 00:00:00

abstract：:The complete sequencing of the Mycobacterium tuberculosis genome offers a unique opportunity to fully elucidate the biology of this human pathogen. One aspect of significant importance is the definition of T cell antigens. This report describes the development and implementation of a proteomic approach to defining suc...

journal_title：Proteomics

authors： Covert BA,Spencer JS,Orme IM,Belisle JT

更新日期：2001-04-01 00:00:00

abstract：:We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...

journal_title：Proteomics

authors： Cramer R,Corless S

更新日期：2005-02-01 00:00:00

abstract：:Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A4...

journal_title：Proteomics

authors： Castagna A,Antonioli P,Astner H,Hamdan M,Righetti SC,Perego P,Zunino F,Righetti PG

更新日期：2004-10-01 00:00:00

abstract：:Secreted proteins play a pivotal role in cellular functions. To better understand malignant behavior, we adapted stable isotopic labeling with amino acids in cell culture technology to identify and quantify proteins differentially released into the extracellular media by a pair of normal and malignant breast-cancer ce...

journal_title：Proteomics

authors： Liang X,Huuskonen J,Hajivandi M,Manzanedo R,Predki P,Amshey JR,Pope RM

更新日期：2009-01-01 00:00:00

abstract：:A protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. The chip was developed for the ArrayTube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich...

journal_title：Proteomics

authors： Huelseweh B,Ehricht R,Marschall HJ

更新日期：2006-05-01 00:00:00

abstract：:Identification and characterization of biomarkers in body fluids such as serum or urine serve as a basis for early detection of diseases, particularly of cancer. Performing 2-DE with subsequent MS analyses, conventional immunoblotting and immunohistochemistry we identified two proteins, orosomucoid (ORM) and human zin...

journal_title：Proteomics

authors： Irmak S,Tilki D,Heukeshoven J,Oliveira-Ferrer L,Friedrich M,Huland H,Ergün S

更新日期：2005-11-01 00:00:00

abstract：:This is the first broad proteomic description of Gluconacetobacter diazotrophicus, an endophytic bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in tropical regions. Proteomic coverage of G. diazotrophicus PAL5 was obtained by two independent approaches: 2-DE followed by MA...

journal_title：Proteomics

authors： Lery LM,Coelho A,von Kruger WM,Gonçalves MS,Santos MF,Valente RH,Santos EO,Rocha SL,Perales J,Domont GB,Teixeira KR,Bertalan M,Ferreira PC,Bisch PM

更新日期：2008-04-01 00:00:00

abstract：:Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...

journal_title：Proteomics

authors： Corrigan L,Jefferies C,Clive Lee T,Daly J

更新日期：2011-08-01 00:00:00

abstract：:Integral membrane proteins (IMPs) perform crucial cellular functions and are the primary targets for most pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains and their intimate association with lipids make them difficult to handle. Numerous proteomic platforms that include LC sepa...

journal_title：Proteomics

authors： Blackler AR,Speers AE,Wu CC

更新日期：2008-10-01 00:00:00

abstract：:Automated multidimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly applied in various large scale proteome profiling efforts. However, comprehensive global proteome analysis remains technically challenging due to issues associated with sample complexity and dynamic ran...

journal_title：Proteomics

authors： Liu T,Qian WJ,Chen WN,Jacobs JM,Moore RJ,Anderson DJ,Gritsenko MA,Monroe ME,Thrall BD,Camp DG 2nd,Smith RD

更新日期：2005-04-01 00:00:00

abstract：:The quality of samples and of pre-analytical steps are crucial in all biological tests, this is dramatically true in proteomics analysis. In renal cell carcinoma preparation for two-dimensional gel electrophoresis the time elapsed between sample collection and treatment, and the heterogeneity of tissues are considered...

journal_title：Proteomics

authors： Sarto C,Valsecchi C,Mocarelli P

更新日期：2002-11-01 00:00:00

abstract：:MALDI-MS Imaging is a novel label-free technique that can be used to visualize the changes in multiple mass responses following treatment. Following treatment with proinflammatory cytokine interleukin-22 (IL-22), the epidermal differentiation of Labskin, a living skin equivalent (LSE), successfully modeled psoriasis i...

journal_title：Proteomics

authors： Harvey A,Cole LM,Day R,Bartlett M,Warwick J,Bojar R,Smith D,Cross N,Clench MR

更新日期：2016-06-01 00:00:00

abstract：:Heat shock proteins (HSP) are families of highly conserved proteins which are induced in cells and tissues upon exposure to extreme conditions causing acute or chronic stress. They exhibit distinct functions and have been implicated in the pathogenesis of a number of diseases, including cancer. A causal relationship b...

journal_title：Proteomics

authors： Lichtenfels R,Kellner R,Bukur J,Beck J,Brenner W,Ackermann A,Seliger B

更新日期：2002-05-01 00:00:00

abstract：:Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic F. oxysporum strains. In this study, F. oxysporum wild-type (WT) MSA 35, a biocontrol multi...

journal_title：Proteomics

authors： Moretti M,Grunau A,Minerdi D,Gehrig P,Roschitzki B,Eberl L,Garibaldi A,Gullino ML,Riedel K

更新日期：2010-09-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is the second cause of cancer-related deaths worldwide. A clearer understanding of the molecular mechanisms underlying tumor growth and invasiveness remains crucial for developing new therapies. Here, the expression of tetraspanins, a family of plasma membrane organizers involved in tumo...

journal_title：Proteomics

authors： Sidahmed-Adrar N,Ottavi JF,Benzoubir N,Ait Saadi T,Bou Saleh M,Mauduit P,Guettier C,Desterke C,Le Naour F

更新日期：2019-11-01 00:00:00

abstract：:Niemann-Pick disease, type C1 (NPC1) is a fatal, autosomal recessive, neurodegenerative disorder caused by mutations in the NPC1 gene. As a result of the genetic defect, there is accumulation of unesterified cholesterol and sphingolipids in the late endosomal/lysosomal system causing both visceral and neurological def...

journal_title：Proteomics

authors： Pergande MR,Serna-Perez F,Mohsin SB,Hanek J,Cologna SM

更新日期：2019-09-01 00:00:00

abstract：:The soluble fraction of nuclear proteins is a functionally significant fraction, since it has been shown that it contains ribonucleoproteins active in nuclear RNA metabolism. The aim of this work was to detect variations associated with cell proliferation, by comparing two-dimensional proteomes obtained from the solub...

journal_title：Proteomics

authors： González-Camacho F,Medina FJ

更新日期：2004-02-01 00:00:00

abstract：:Ovarian cancer is one of the leading causes of mortality by gynecological cancer. Despite good response to surgery and initial chemotherapy, essentially based on cisplatin (cis-diamino-dichloro-platinum(II) (CDDP)) compounds, frequent recurrences with chemoresistance acquisition are responsible for poor prognosis. Sev...

journal_title：Proteomics

authors： Le Moguen K,Lincet H,Marcelo P,Lemoisson E,Heutte N,Duval M,Poulain L,Vinh J,Gauduchon P,Baudin B

更新日期：2007-11-01 00:00:00

abstract：:Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic func...

journal_title：Proteomics

authors： Guergues J,Zhang P,Liu B,Stevens SM Jr

更新日期：2019-06-01 00:00:00

abstract：:Proteins extracted from leaf blades of rice plants infected with blast fungus, Magnaporthe grisea, were separated by two-dimensional polyacrylamide gel electrophoresis. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane, and 63 proteins were analyzed by a gas-phase protein sequencer. ...

journal_title：Proteomics

authors： Konishi H,Ishiguro K,Komatsu S

更新日期：2001-09-01 00:00:00

abstract：:The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by d...

journal_title：Proteomics

authors： Schaumburg J,Diekmann O,Hagendorff P,Bergmann S,Rohde M,Hammerschmidt S,Jänsch L,Wehland J,Kärst U

更新日期：2004-10-01 00:00:00

abstract：:Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. Ho...

journal_title：Proteomics

authors： Zavan L,Bitto NJ,Johnston EL,Greening DW,Kaparakis-Liaskos M

更新日期：2019-01-01 00:00:00

abstract：:Identification of mucin-type O-glycosylated proteins with known functions in model organisms like Drosophila could provide keys to elucidate functions of the O-glycan moiety and proteomic analyses of O-glycoproteins in higher eukaryotes remain a challenge due to structural heterogeneity and a lack of efficient tools f...

journal_title：Proteomics

authors： Schwientek T,Mandel U,Roth U,Müller S,Hanisch FG

更新日期：2007-09-01 00:00:00

abstract：:A diverse range of proteins are modified by the post-translational covalent attachment of a glycosyl-phosphatidylinositol (GPI) membrane anchor. Hydropathy plots and other computer algorithms can be used to predict the presence of a GPI anchor attachment signal in the nascent polypeptide chain. However, the presence o...

journal_title：Proteomics

authors： Hooper NM

更新日期：2001-06-01 00:00:00

abstract：:The hypothalamus is the central regulatory unit that balances a number of body functions including metabolic rate, hunger, and satiety signals. Hypothalamic neurons monitor and respond to alterations of circulating nutrients and hormones that reflect the peripheral energy status. These extracellular signals are integr...

journal_title：Proteomics

authors： Kuhla B,Kuhla S,Rudolph PE,Albrecht D,Metges CC

更新日期：2007-10-01 00:00:00