pseudoQC: A Regression-Based Simulation Software for Correction and Normalization of Complex Metabolomics and Proteomics Datasets.

abstract：:Porcine follicular fluid (pFF) constitutes the micro-environment of the maturing oocyte. Although pFF is a transudate of serum, in pigs, it is superior to serum in promoting in vitro expansion of the cumulus cells, a specialized cell population surrounding the oocyte. A comparative proteome analysis of autologous seru...

journal_title：Proteomics

authors： Bijttebier J,Tilleman K,Dhaenens M,Deforce D,Van Soom A,Maes D

更新日期：2009-10-01 00:00:00

abstract：:Egr1 (Zif268) is an immediate early gene encoding an inducible transcription factor involved in synaptic plasticity and several forms of memory in rodents. Using 2-DE and MS, we compared proteomes of hippocampal subregions and cortex in Egr1-deficient and wild-type littermates. Two significant differences were identif...

journal_title：Proteomics

authors： Chardonnet S,Decottignies P,Amar L,Le Caer JP,Davis S,Laroche S,Le Maréchal P

更新日期：2007-01-01 00:00:00

abstract：:The Japanese red and pink corals are known to be precious because of their commercial value resulting from their use in ornaments, jewelry, and medicine. Precious corals are very interesting models for biomineralization studies and possess two different skeletal structures: an axial skeleton and an endoskeleton (scler...

journal_title：Proteomics

authors： Rahman MA,Karl K,Nonaka M,Fujimura H,Shinjo R,Oomori T,Wörheide G

更新日期：2014-11-01 00:00:00

abstract：:Protein arginine methylation is a PTM catalyzed by an evolutionarily conserved family of enzymes called protein arginine methyltransferases (PRMTs), with PRMT1 being the most conserved member of this enzyme family. This modification has emerged to be an important regulator of protein functions. To better understand th...

journal_title：Proteomics

authors： Jackson CA,Yadav N,Min S,Li J,Milliman EJ,Qu J,Chen YC,Yu MC

更新日期：2012-11-01 00:00:00

abstract：:Despite affecting up to 70% of HIV-positive patients and being the leading cause of dementia in patients under 40 years, the molecular mechanisms involved in the onset of HIV-associated neurocognitive disorders (HAND) are not well understood. To address this, we performed SILAC-based quantitative proteomic analysis on...

journal_title：Proteomics

authors： Ganief T,Gqamana P,Garnett S,Hoare J,Stein DJ,Joska J,Soares N,Blackburn JM

更新日期：2017-03-01 00:00:00

abstract：:Mitochondria were isolated from whole hearts of Dahl salt sensitive (SS) and chromosome 13 consomic control (SS.13BN/Mcwi) rats using a mechanical homogenization process followed by density centrifugation. The proteins present in the two mitochondria preparations were quantified; equal amounts of protein from each sam...

journal_title：Proteomics

authors： Smith JR,Matus IR,Beard DA,Greene AS

更新日期：2008-02-01 00:00:00

abstract：:Peripheral nerve injury may lead to neuroadaptive changes of cellular signals in spinal cord that are thought to contribute to central mechanisms underlying neuropathic pain. Here we used a 2-DE-based proteomic technique to determine the global expression changes of synaptosome-associated proteins in spinal cord dorsa...

journal_title：Proteomics

authors： Singh OV,Yaster M,Xu JT,Guan Y,Guan X,Dharmarajan AM,Raja SN,Zeitlin PL,Tao YX

更新日期：2009-03-01 00:00:00

abstract：:The flavonoid flavone contained in a variety of fruits and vegetables was identified as a very potent apoptosis inducer in human colonic cancer cells. In search of the molecular targets of flavone action in HT-29 cells we analyzed changes in mRNA and protein expression levels by proteomics and oligonucleotide array te...

journal_title：Proteomics

authors： Herzog A,Kindermann B,Döring F,Daniel H,Wenzel U

更新日期：2004-08-01 00:00:00

abstract：:Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...

journal_title：Proteomics

authors： Westers L,Westers H,Zanen G,Antelmann H,Hecker M,Noone D,Devine KM,van Dijl JM,Quax WJ

更新日期：2008-07-01 00:00:00

abstract：:The discovery of PTMs in proteins by MS requires nearly complete sequence coverage of the detected proteolytic peptides. Unfortunately, mass spectrometric analysis of the desired sequence fragments is often impeded due to low ionization efficiency and/or signal suppression in complex samples. When several lysine resid...

journal_title：Proteomics

authors： Markoutsa S,Bahr U,Papasotiriou DG,Häfner AK,Karas M,Sorg BL

更新日期：2014-03-01 00:00:00

abstract：:Prevotella intermedia binds and invades a variety of host cells. This binding is most probably mediated through cell surface proteins termed adhesins. To identify proteins binding to the host extracellular matrix (ECM) component, fibronectin, and study the molecular mechanism underlying bacterial colonization, we appl...

journal_title：Proteomics

authors： Yu F,Iyer D,Anaya C,Lewis JP

更新日期：2006-11-01 00:00:00

abstract：:Panax ginseng is an important herb that has clear effects on the treatment of diverse diseases. Until now, the natural peptide constitution of this herb remains unclear. Here, we conduct an extensive characterization of Ginseng peptidome using MS-based data mining and sequencing. The screen on the charge states of pre...

journal_title：Proteomics

authors： Ye X,Zhao N,Yu X,Han X,Gao H,Zhang X

更新日期：2016-11-01 00:00:00

abstract：:Quantitative proteomics technology based on isobaric tags is playing an important role in proteomic investigation. In this paper, we present an automated software, named IQuant, which integrates a postprocessing tool of protein identification and advanced statistical algorithms to process the MS/MS signals generated f...

journal_title：Proteomics

authors： Wen B,Zhou R,Feng Q,Wang Q,Wang J,Liu S

更新日期：2014-10-01 00:00:00

abstract：:We report on a novel strategy for identification of specific sulfation motifs in chondroitin/dermatan sulfate (CS/DS) chain derived from decorin (Dcn), based on enzyme cleavage and multistage MS (MS(n)). Released CS/DS chains were digested with chondroitin B and in parallel with AC I lyases to obtain oligosaccharides ...

journal_title：Proteomics

authors： Zamfir AD,Flangea C,Sisu E,Serb AF,Dinca N,Bruckner P,Seidler DG

更新日期：2009-07-01 00:00:00

abstract：:Daphnia pulex is a keystone species for aquatic habitats and an ecological/evolution model organism. Although significant progress has been made on characterizing its genome, the D. pulex proteome remains largely uncharacterized partially due to abnormally high protein degradation during homogenization and emphasis on...

journal_title：Proteomics

authors： Steevensz A,Gombar R,Vergilino R,Cristescu ME,Vacratsis PO

更新日期：2018-08-01 00:00:00

abstract：:In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of adipocyte-secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of murine differentiated 3T3-L1 adipocytes ...

journal_title：Proteomics

authors： Laria AE,Messineo S,Arcidiacono B,Varano M,Chiefari E,Semple RK,Rocha N,Russo D,Cuda G,Gaspari M,Brunetti A,Foti DP

更新日期：2018-04-01 00:00:00

abstract：:New insights on cellular heterogeneity in the last decade provoke the development of a variety of single cell omics tools at a lightning pace. The resultant high-dimensional single cell data generated by these tools require new theoretical approaches and analytical algorithms for effective visualization and interpreta...

journal_title：Proteomics

authors： Su Y,Shi Q,Wei W

更新日期：2017-02-01 00:00:00

abstract：:FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...

journal_title：Proteomics

authors： Gattiker A,Bienvenut WV,Bairoch A,Gasteiger E

更新日期：2002-10-01 00:00:00

abstract：:Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60 min at 8...

journal_title：Proteomics

authors： Egan B,Dowling P,O'Connor PL,Henry M,Meleady P,Zierath JR,O'Gorman DJ

更新日期：2011-04-01 00:00:00

abstract：:Intrinsic or acquired resistance to vincristine (VCR), an antimicrotubule agent used in the treatment of childhood acute lymphoblastic leukemia (ALL), is a major clinical problem. Using a clinically relevant NOD/SCID mouse xenograft model of ALL, we established that alterations in the actin and tubulin cytoskeleton ar...

journal_title：Proteomics

authors： Verrills NM,Liem NL,Liaw TY,Hood BD,Lock RB,Kavallaris M

更新日期：2006-03-01 00:00:00

abstract：:Along with unequivocal hits produced by matching multiple MS/MS spectra to database sequences, LC-MS/MS analysis often yields a large number of hits of borderline statistical confidence. To simplify their validation, we propose to use rapid de novo interpretation of all acquired MS/MS spectra and, with the help of a s...

journal_title：Proteomics

authors： Thomas H,Shevchenko A

更新日期：2008-10-01 00:00:00

abstract：:The endothelium is a single layer of cells lining the inside face of all blood vessels. It constitutes a major metabolic organ which is critically involved in the generation and the regulation of multiple physiological and pathological processes such as coagulation, hemostasis, inflammation, atherosclerosis, angiogene...

journal_title：Proteomics

authors： Bruneel A,Labas V,Mailloux A,Sharma S,Vinh J,Vaubourdolle M,Baudin B

更新日期：2003-05-01 00:00:00

abstract：:The HUPO Brain Proteome Project (HUPO BPP) held its 12th workshop in Toronto on 26 September 2009 prior to the HUPO VIII World Congress. The principal aim of this project is to obtain a better understanding of neurodiseases and ageing, with the ultimate objective of discovering prognostic and diagnostic biomarkers, in...

journal_title：Proteomics

authors： Gröttrup B,Eisenacher M,Stephan C,Marcus K,Lee B,Meyer HE,Park YM

更新日期：2010-06-01 00:00:00

abstract：:Colorectal cancer risk is increased when dietary folate intake is low, with or without a deficiency in methylenetetrahydrofolate reductase (MTHFR). We have observed that intestinal tumors are induced in mice fed low-folate diets, and that tumor incidence is increased when these mice also have MTHFR deficiency. This st...

journal_title：Proteomics

authors： Leclerc D,Dejgaard K,Mazur A,Deng L,Wu Q,Nilsson T,Rozen R

更新日期：2014-11-01 00:00:00

abstract：:The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was dev...

journal_title：Proteomics

authors： Jung SO,Ro HS,Kho BH,Shin YB,Kim MG,Chung BH

更新日期：2005-11-01 00:00:00

abstract：:Cancer cells maintain their intracellular ROS concentrations at required levels for their survival. Changes in ROS concentrations can regulate biochemical signaling mechanisms that control cell function. It has been demonstrated that ROS regulate the cellular events through redox regulation of redox-sensitive proteins...

journal_title：Proteomics

authors： Yuan K,Liu Y,Chen HN,Zhang L,Lan J,Gao W,Dou Q,Nice EC,Huang C

更新日期：2015-01-01 00:00:00

abstract：:Clostridium cellulolyticum is a model mesophilic anaerobic bacterium that efficiently degrades plant cell walls. The recent genome release offers the opportunity to analyse its complete degradation system. A total of 148 putative carbohydrate-active enzymes were identified, and their modular structures and activities ...

journal_title：Proteomics

authors： Blouzard JC,Coutinho PM,Fierobe HP,Henrissat B,Lignon S,Tardif C,Pagès S,de Philip P

更新日期：2010-02-01 00:00:00

abstract：:The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...

journal_title：Proteomics

authors： Marengo E,Robotti E,Antonucci F,Cecconi D,Campostrini N,Righetti PG

更新日期：2005-02-01 00:00:00

abstract：:In this work, for the first time, a novel C60-functionalized magnetic silica microsphere (designated C60-f-MS) was synthesized by radical polymerization of C60 molecules on the surface of magnetic silica microspheres. The resulting C60-f-MS microsphere has magnetite core and thin C60 modified silica shell, which endow...

journal_title：Proteomics

authors： Chen H,Qi D,Deng C,Yang P,Zhang X

更新日期：2009-01-01 00:00:00

abstract：:The basic problem of complexity poses a significant challenge for proteomic studies. To date two-dimensional gel electrophoresis (2-DE) followed by enzymatic in-gel digestion of the peptides, and subsequent identification by mass spectrometry (MS) is the most commonly used method to analyze complex protein mixtures. H...

journal_title：Proteomics

authors： Ihling C,Sinz A

更新日期：2005-05-01 00:00:00