Abstract:
:Changing smooth muscle phenotype and abnormal cell proliferation are important features of vascular pathology, including the failure of saphenous vein bypass grafts. We have characterised and mapped protein expression in human saphenous vein medial smooth muscle, using two-dimensional (2-D) polyacrylamide gel electrophoresis. The 2-D system comprised a nonlinear immobilised pH 3-10 gradient in the first dimension (separating proteins with isoelectric point values between pH 3-10), and 12%T total gel concentration sodium dodecyl sulphate polyacrylamide gel electrophoresis in the second dimension (separating proteins in the range 14,000-200,000 Daltons). Using a combination of peptide mass fingerprinting by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry and partial amino acid sequencing by nanospray tandem mass spectrometry, a subset of 149 protein spots was analysed, with 129 protein spots being identified and mapped. The data presented here are an important addition to the limited knowledge of venous medial smooth muscle protein expression in vivo. Our protein map will facilitate the identification of proteins differentially expressed in human saphenous vein bypass grafts. In turn, this may lead to the elucidation of molecular events involved in saphenous vein bypass graft failure. The map should also provide a basis for comparative studies of protein expression in vascular smooth muscle of varying origins.
journal_name
Proteomicsjournal_title
Proteomicsauthors
McGregor E,Kempster L,Wait R,Welson SY,Gosling M,Dunn MJ,Powel JTdoi
10.1002/1615-9861(200111)1:11<1405::AID-PROT1405>3subject
Has Abstractpub_date
2001-11-01 00:00:00pages
1405-14issue
11eissn
1615-9853issn
1615-9861journal_volume
1pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Gliadins and glutenins are the main protein fractions present in wheat gluten. They are responsible for technological and nutritional quality of wheat based products. In particular, glutenins are mainly responsible for dough visco-elastic properties, whereas gliadins confer extensibility to dough and are the most impo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500236
更新日期:2006-03-01 00:00:00
abstract::In this study, we generated 40 somatic cell cloned (scNT) piglets. Of these, five piglets were stillborn, 22 scNT piglets died suddenly within the first week of life, and 1 piglet died after 40 days. Twelve scNT piglets are still healthy. The birth weights of compromised scNT piglets in comparison with those of normal...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401079
更新日期:2005-05-01 00:00:00
abstract::Non-DNA microarrays, such as protein, peptide and small molecule microarrays, can potentially revolutionize the high-throughput screening tools currently used in basic and pharmaceutical research. However, fundamental obstacles remain that limit their rapid and widespread implementation as an alternative bioanalytical...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401078
更新日期:2005-05-01 00:00:00
abstract::Aluminum (Al) toxicity is a major constraint for plant root development and growth as well as crop yield in acidic soils, which constitute approximately 40% of the potentially arable lands worldwide. The mechanisms of Al tolerance in plants are not well understood. As a whole systems approach, proteomic techniques hav...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201300252
更新日期:2014-03-01 00:00:00
abstract::Currently, there are few predictive biomarkers in key biomonitoring species, such as oysters, that can detect heavy metal pollution in coastal waterways. Several attributes make oysters superior to other organisms for positive biomonitoring of heavy metal pollution. In particular, they are filter feeders with a high c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100417
更新日期:2012-03-01 00:00:00
abstract::Human saliva contains a large number of proteins and peptides (salivary proteome) that help maintain homeostasis in the oral cavity. Global analysis of human salivary proteome is important for understanding oral health and disease pathogenesis. In this study, large-scale identification of salivary proteins was demonst...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401037
更新日期:2005-04-01 00:00:00
abstract::The eukaryotic chromatin/chromosome stores genomic information, controls genetic material distribution, and plays an essential role in the establishment and maintenance of spatial and temporal gene expression profile. Despite over a century of research, the protein composition and higher level structure of chromatin s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700580
更新日期:2007-12-01 00:00:00
abstract::Cnidarians are widely distributed basal metazoans that play an important role in the marine ecosystem. Their genetic diversity and dispersal depends on successful oogenesis, fertilization and embryogenesis. To understand the processes that lead to successful embryogenesis in these basal organisms, we conducted compara...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500255
更新日期:2015-12-01 00:00:00
abstract::To better understand the mechanism underlying hepatocellular carcinoma (HCC) metastasis and to search for potential markers for HCC prognosis, differential proteome analysis on two HCC cell strains with high and low metastatic potentials, MHCC97-H and MHCC97-L, was conducted using two-dimensional (2-D) gel electrophor...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300653
更新日期:2004-04-01 00:00:00
abstract::CD28 delivers a co-stimulatory signal for T cell antigen receptor induced activation of T cells through a mechanism which remains mostly elusive to date. In order to try and gain insight into CD28 function, we therefore applied state-of-the-art mass spectrometric protein identification technology to the analysis of CD...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200101)1:1<70::AID-PROT70>3.0.CO
更新日期:2001-01-01 00:00:00
abstract::We present MassSieve, a Java-based platform for visualization and parsimony analysis of single and comparative LC-MS/MS database search engine results. The success of mass spectrometric peptide sequence assignment algorithms has led to the need for a tool to merge and evaluate the increasing data set sizes that result...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900370
更新日期:2010-08-01 00:00:00
abstract::The allergen content of standardized pollen material is crucial for an effective diagnosis and treatment. However, variations in IgE reactivities of allergic patients to different preparations of Phleum pratense pollen have been reported. In order to define and directly compare the allergen composition of pollen prepa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000451
更新日期:2010-12-01 00:00:00
abstract::Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000548
更新日期:2011-03-01 00:00:00
abstract::In this work, for the first time, perfluorinated magnetic mesoporous microspheres were designed and synthesized for the highly specific enrichment of fluorous-derivatized phosphopeptides through the unique fluorine-fluorine interactions. The perfluorinated magnetic mesoporous microspheres were prepared through a surfa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500323
更新日期:2016-04-01 00:00:00
abstract::In mass spectrometry-based proteomics, protein identification results usually consist of peptide sequences and database-dependent accession identifiers of the matching proteins. Often certain annotations are only available in particular databases that in turn must be queried by a certain identifier. In order to simpli...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600018
更新日期:2006-08-01 00:00:00
abstract::The activation of caspases is a critical event for the execution phase of programmed cell death. Caspases are highly specific in their ability to activate or inhibit many crucial proteins in the cell via site-specific cleavage. To date, more than 60 proteins have been shown to be substrates of one or more caspases in ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400979
更新日期:2004-11-01 00:00:00
abstract::Total parenteral nutrition (TPN) is provided as the primary nitrogen source to manage patients with intestinal failure who were not able to sustain themselves on enteral feeds. The most common complication of long-term TPN use is hepatitis. A proteomic approach was used to identify proteins that are differentially exp...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500128
更新日期:2015-11-01 00:00:00
abstract::FindPept (http://www.expasy.org/tools/findpept.html) is a software tool designed to identify the origin of peptide masses obtained by peptide mass fingerprinting which are not matched by existing protein identification tools. It identifies masses resulting from unspecific proteolytic cleavage, missed cleavage, proteas...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200210)2:10<1435::AID-PROT1435>3
更新日期:2002-10-01 00:00:00
abstract::Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300465
更新日期:2003-10-01 00:00:00
abstract::Due to their extraordinary capacity to hypertolerate and hyperaccumulate heavy metals in above-ground tissues, hyperaccumulator plant species have gained wide attention from researchers seeking biotechnologies to manage environmental heavy metal pollution. However, the molecular basis of hyperaccumulation is still far...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201600456
更新日期:2017-05-01 00:00:00
abstract::The glycan structures of the major and more than ten minor populated isoforms of antithrombin (AT) were determined after separation of the isoforms by IEF using IPG strips. The bands excised from the gel were reduced, derivatized by iodoacetamide and submitted to tryptic digestion. The digest was analyzed by RP-HPLC-E...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401238
更新日期:2005-10-01 00:00:00
abstract::MS protein identification and quantitation are key proteomic techniques in biological research. Besides identification of proteins, MS is used increasingly to characterize secondary protein modifications. This often requires trimming the analytical strategy to a specific type of modification. Direct analysis of protei...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200501332
更新日期:2006-01-01 00:00:00
abstract::Intrinsically disordered proteins (IDPs) are implicated in a range of human diseases, some of which are associated with the ability to bind to lipids. Although the presence of solvent-exposed hydrophobic regions in IDPs should favor their interactions with low-molecular-weight hydrophobic/amphiphilic compounds, this h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800098
更新日期:2019-03-01 00:00:00
abstract::The 26S proteasome is a multicatalytic protease complex that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core (the 20S proteasome) as well as regulatory particles, which contain six ATPase (Rpt) subunits involved in unfolding and translocation of substrates to the catalytic chambe...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300207
更新日期:2013-11-01 00:00:00
abstract::More than 300 different types of protein post-translational modifications (PTMs) have been described, many of which are known to have pivotal roles in cellular physiology and disease. Nevertheless, only a handful of PTMs have been extensively investigated at the proteome level. Knowledge of protein substrates and thei...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200900398
更新日期:2009-10-01 00:00:00
abstract::Sarcopenia, defined as age-associated decline of muscle mass and function, is a risk factor for mortality and disability, and comorbid with several chronic diseases such as type II diabetes and cardiovascular diseases. Clinical trials showed that nutritional supplements had positive effects on muscle mass, but not on ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800411
更新日期:2020-03-01 00:00:00
abstract::Identification of differential proteomic responses to cadmium (Cd) would provide a means for better understanding the survival mechanisms of the earthworm, Eisenia fetida, living in a Cd-polluted environment, and the stress responses can be assessed by ecotoxiptoromics approaches. Extracts of whole earthworm collected...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000209
更新日期:2010-12-01 00:00:00
abstract::In the postgenomic era, many researchers and organizations have been engaged in structural and functional analyses of proteins. As a part of these efforts, searching for small organic compounds that bind specifically to target proteins is quite important. In this study, we have developed a rational strategy for ligand...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401032
更新日期:2005-04-01 00:00:00
abstract::The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic dig...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000039
更新日期:2010-07-01 00:00:00
abstract::Sequencing of the human genome opened the way to the exploration of the proteome and this has lead to the identification of large numbers of proteins in complex biological samples. The identification of diagnostic patterns in samples taken from patients to aid diagnosis is in the early stages of development. The solut...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700665
更新日期:2008-02-01 00:00:00