A proteomic approach to verify in vivo expression of a novel gamma-gliadin containing an extra cysteine residue.

abstract：:Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro-organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance ...

journal_title：Proteomics

authors： Christie-Oleza JA,Scanlan DJ,Armengaud J

更新日期：2015-10-01 00:00:00

abstract：:Mitochondria were isolated from whole hearts of Dahl salt sensitive (SS) and chromosome 13 consomic control (SS.13BN/Mcwi) rats using a mechanical homogenization process followed by density centrifugation. The proteins present in the two mitochondria preparations were quantified; equal amounts of protein from each sam...

journal_title：Proteomics

authors： Smith JR,Matus IR,Beard DA,Greene AS

更新日期：2008-02-01 00:00:00

abstract：:Trichomonas vaginalis, a sexually transmitted parasite, has many cysteine proteinases (CPs); some are involved in trichomonal pathogenesis, express during infection, and antibodies against CPs have been detected in patient sera. The goal of this study was to identify the antigenic proteinases of T. vaginalis as potent...

journal_title：Proteomics

authors： Ramón-Luing LA,Rendón-Gandarilla FJ,Cárdenas-Guerra RE,Rodríguez-Cabrera NA,Ortega-López J,Avila-González L,Angel-Ortiz C,Herrera-Sánchez CN,Mendoza-García M,Arroyo R

更新日期：2010-02-01 00:00:00

abstract：:We introduce a new methodology named ligand profiling and identification for effective discovery of bioactive ligands such as peptide hormones. This technology was developed from a new concept of parallel column chromatography and active fraction profiling by nano-LC MS. Traditional methods use sequential column chrom...

journal_title：Proteomics

authors： Baek MC,Kim SJ,Yea K,Kim Y,Lee BD,Kim J,Lee HJ,Kang MH,Choi SK,Kim JI,Lee TG,Suh PG,Ryu SH

更新日期：2006-03-01 00:00:00

abstract：:Helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. In addition H. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are a...

journal_title：Proteomics

authors： Hynes SO,McGuire J,Falt T,Wadström T

更新日期：2003-03-01 00:00:00

abstract：:The sample preparation procedures established for Prorocentrum triestinum were adapted to cover both thecate and athecate dinoflagellates. Further, whether trichloroacetic acid (TCA) precipitation can be used to fix and preserve the harmful or nuisance species from local waters that they infest was tested. Optimized t...

journal_title：Proteomics

authors： Chan LL,Hodgkiss IJ,Lu S,Lo SC

更新日期：2004-01-01 00:00:00

abstract：:Abnormalities of the anterior cingulate cortex have previously been described in schizophrenia, major depressive disorder and bipolar disorder. In this study 2-DE was performed followed by mass spectrometric sequencing to identify disease-specific protein changes within the anterior cingulate cortex in these psychiatr...

journal_title：Proteomics

authors： Beasley CL,Pennington K,Behan A,Wait R,Dunn MJ,Cotter D

更新日期：2006-06-01 00:00:00

abstract：:This study was undertaken to identify growth hormone (GH) responsive proteins and protein expression patterns by short-term recombinant human growth hormone (rhGH) therapy in patients with idiopathic short stature (ISS) using proteomic analysis. Seventeen children (14 males and three females) with ISS were included. T...

journal_title：Proteomics

authors： Heo SH,Choi JH,Kim YM,Jung CW,Lee J,Jin HY,Kim GH,Lee BH,Shin CH,Yoo HW

更新日期：2013-04-01 00:00:00

abstract：:Large-scale interaction studies contribute the largest fraction of protein interactions information in databases. However, co-purification of non-specific or indirect ligands, often results in data sets that are affected by a considerable number of false positives. For the fraction of interactions mediated by short li...

journal_title：Proteomics

authors： Panni S,Montecchi-Palazzi L,Kiemer L,Cabibbo A,Paoluzi S,Santonico E,Landgraf C,Volkmer-Engert R,Bachi A,Castagnoli L,Cesareni G

更新日期：2011-01-01 00:00:00

abstract：:Aging is associated with a progressive loss of skeletal muscular function that often leads to progressive disability and loss of independence. Although muscle aging is well documented, the molecular mechanisms of this condition still remain unclear. To gain greater insight into the changes associated with aging of ske...

journal_title：Proteomics

authors： Hwang CY,Kim K,Choi JY,Bahn YJ,Lee SM,Kim YK,Lee C,Kwon KS

更新日期：2014-01-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a major complication of chronic viral hepatitis C. Therapy for HCC is still disappointing. It is thus of great importance to identify novel HCC markers for early detection of the disease, and tumor-specific proteins as potential therapeutic targets. We have used a proteomic approach t...

journal_title：Proteomics

authors： Blanc JF,Lalanne C,Plomion C,Schmitter JM,Bathany K,Gion JM,Bioulac-Sage P,Balabaud C,Bonneu M,Rosenbaum J

更新日期：2005-09-01 00:00:00

abstract：:In 2001, the German Federal Ministry of Education and Research (BMBF) initiated the National Genome Research Network (NGFN; www.ngfn.de) as a nation-wide multidisciplinary networking platform aiming at the analysis of common human diseases and aging. Within the NGFN the Human Brain Proteome Project (HBPP; www.smp-prot...

journal_title：Proteomics

authors： Hamacher M,Hardt T,van Hall A,Stephan C,Marcus K,Meyer HE

更新日期：2008-03-01 00:00:00

abstract：:Arabidopsis thaliana cell suspension cultures have been used to investigate the effects of salinity and hyperosmotic stress on plant cellular proteins. We show that 200 mM NaCl and 400 mM sorbitol treatments induce extracellular medium acidification in Arabidopsis cell cultures, a typical response of plant cells to sa...

journal_title：Proteomics

authors： Ndimba BK,Chivasa S,Simon WJ,Slabas AR

更新日期：2005-11-01 00:00:00

abstract：:The Annual Spring workshop of the HUPO-PSI was this year held at the EMBL International Centre for Advanced Training (EICAT) in Heidelberg, Germany. Delegates briefly reviewed the successes of the group to date. These include the wide spread implementation of the molecular interaction data exchange formats, PSI-MI XML...

journal_title：Proteomics

authors： Orchard S,Albar JP,Deutsch EW,Eisenacher M,Vizcaíno JA,Hermjakob H

更新日期：2011-11-01 00:00:00

abstract：:The array of biomolecules generated by a functioning ecosystem represents both a potential resource for sustainable harvest and a potential indicator of ecosystem health and function. The cupped leaves of the carnivorous pitcher plant, Sarracenia purpurea, harbor a dynamic food web of aquatic invertebrates in a fully ...

journal_title：Proteomics

authors： Gotelli NJ,Smith AM,Ellison AM,Ballif BA

更新日期：2011-06-01 00:00:00

abstract：:Proteins extracted from leaf blades of rice plants infected with blast fungus, Magnaporthe grisea, were separated by two-dimensional polyacrylamide gel electrophoresis. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane, and 63 proteins were analyzed by a gas-phase protein sequencer. ...

journal_title：Proteomics

authors： Konishi H,Ishiguro K,Komatsu S

更新日期：2001-09-01 00:00:00

abstract：:Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 microM Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification....

journal_title：Proteomics

authors： Kieffer P,Dommes J,Hoffmann L,Hausman JF,Renaut J

更新日期：2008-06-01 00:00:00

abstract：:During clinical mastitis in dairy cows, the quantity of milk produced decreases and the composition of the milk is altered. As the severity of inflammation associated with the disease increases, the chemical composition of milk approaches that of blood as a consequence of increased permeability of the blood mammary ba...

journal_title：Proteomics

authors： Hogarth CJ,Fitzpatrick JL,Nolan AM,Young FJ,Pitt A,Eckersall PD

更新日期：2004-07-01 00:00:00

abstract：:Depletion of abundant proteins is one of the effective ways to improve detection and identification of low-abundance proteins. Our previous study showed that protamine sulfate precipitation (PSP) method can deplete abundant ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from leaf proteins and is suitable fo...

journal_title：Proteomics

authors： Kim YJ,Wang Y,Gupta R,Kim SW,Min CW,Kim YC,Park KH,Agrawal GK,Rakwal R,Choung MG,Kang KY,Kim ST

更新日期：2015-05-01 00:00:00

abstract：:Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. sc...

journal_title：Proteomics

authors： Barnabas L,Ashwin NM,Kaverinathan K,Trentin AR,Pivato M,Sundar AR,Malathi P,Viswanathan R,Rosana OB,Neethukrishna K,Carletti P,Arrigoni G,Masi A,Agrawal GK,Rakwal R

更新日期：2016-04-01 00:00:00

abstract：:The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...

journal_title：Proteomics

authors： Ortsäter H,Sundsten T,Lin JM,Bergsten P

更新日期：2007-09-01 00:00:00

abstract：:Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected by Citrus exocortis viroid (CEVd). Among the differentially expressed proteins detected, 45 were su...

journal_title：Proteomics

authors： Lisón P,Tárraga S,López-Gresa P,Saurí A,Torres C,Campos L,Bellés JM,Conejero V,Rodrigo I

更新日期：2013-03-01 00:00:00

abstract：:Despite increasing evidence of behavioural manipulation of their vectors by pathogens, the underlying mechanisms causing infected vectors to act in ways that benefit pathogen transmission remain enigmatic in most cases. Here, 2-D DIGE coupled with MS were employed to analyse and compare the head proteome of mosquitoes...

journal_title：Proteomics

authors： Lefevre T,Thomas F,Schwartz A,Levashina E,Blandin S,Brizard JP,Le Bourligu L,Demettre E,Renaud F,Biron DG

更新日期：2007-06-01 00:00:00

abstract：:Currently, there are few predictive biomarkers in key biomonitoring species, such as oysters, that can detect heavy metal pollution in coastal waterways. Several attributes make oysters superior to other organisms for positive biomonitoring of heavy metal pollution. In particular, they are filter feeders with a high c...

journal_title：Proteomics

authors： Muralidharan S,Thompson E,Raftos D,Birch G,Haynes PA

更新日期：2012-03-01 00:00:00

abstract：:Two-dimensional difference gel electrophoresis (2-D DIGE) allows for reliable quantification of global protein abundance changes. The threshold of significance for protein abundance changes depends on the experimental variation (biological and technical). This study estimates biological, technical and total variation ...

journal_title：Proteomics

authors： Zech H,Echtermeyer C,Wöhlbrand L,Blasius B,Rabus R

更新日期：2011-08-01 00:00:00

abstract：:The comprehension of sexually different responses in dioecious plants to excess manganese (Mn) stress requires molecular explanation. Physiological and proteomic changes in leaves of Populus cathayana males and females were analyzed after 4 wk of exposure to Mn stress. Under excess Mn conditions, shoot height and phot...

journal_title：Proteomics

authors： Chen F,Zhang S,Zhu G,Korpelainen H,Li C

更新日期：2013-08-01 00:00:00

abstract：:Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...

journal_title：Proteomics

authors： Ahrné E,Müller M,Lisacek F

更新日期：2010-02-01 00:00:00

abstract：:Low-density lipoprotein (LDL) is a major cholesterol carrier in human blood. Oxidations of apolipoprotein B-100 (apo B-100, LDL protein) could be proatherogenic and play critical roles in early stages of plaque formation in the arterial wall. The structure of apo B-100 is still poorly understood, partially due to its ...

journal_title：Proteomics

authors： Chakraborty S,Cai Y,Tarr MA

更新日期：2014-11-01 00:00:00

abstract：:The plenary session of the Proteomics Standards Initiative of the Human Proteome Organisation at the 6th annual HUPO world congress updated the delegates on the current status of the ongoing work of this group. The new MS interchange format, mzML, had been entered into the HUPO document process immediately prior to th...

journal_title：Proteomics

authors： Orchard S,Martens L,Tasman J,Binz PA,Albar JP,Hermjakob H

更新日期：2008-04-01 00:00:00

abstract：:The hypothalamus is the central regulatory unit that balances a number of body functions including metabolic rate, hunger, and satiety signals. Hypothalamic neurons monitor and respond to alterations of circulating nutrients and hormones that reflect the peripheral energy status. These extracellular signals are integr...

journal_title：Proteomics

authors： Kuhla B,Kuhla S,Rudolph PE,Albrecht D,Metges CC

更新日期：2007-10-01 00:00:00