In vitro oxidative footprinting provides insight into apolipoprotein B-100 structure in low-density lipoprotein.

abstract：:Cells are a fundamental unit of life, and the ability to study the phenotypes and behaviors of individual cells is crucial to understanding the workings of complex biological systems. Cell phenotypes (epigenomic, transcriptomic, proteomic, and metabolomic) exhibit dramatic heterogeneity between and within the differen...

journal_title：Proteomics

authors： Mincarelli L,Lister A,Lipscombe J,Macaulay IC

更新日期：2018-09-01 00:00:00

abstract：:In a comparative study, we investigated the influence of nine sample preparation workflows and seven different lysis buffers for qualitative and quantitative analysis of the human adipose tissue proteome. Adipose tissue is not just a fat depot but also an endocrine organ, which cross-talks with other tissue types and ...

journal_title：Proteomics

authors： Pasing Y,Colnoe S,Hansen T

更新日期：2017-03-01 00:00:00

abstract：:With advances in liquid chromatography coupled to tandem mass spectrometry technologies combined with the continued goals of biomarker discovery, clinical applications of established biomarkers, and integrating large multiomic datasets (i.e. "big data"), there remains an urgent need for robust tools to assess instrume...

journal_title：Proteomics

authors： Bereman MS

更新日期：2015-03-01 00:00:00

abstract：:We describe a new approach for the characterization of a digested protein complex with quantitative aspects. Accurate masses of tryptic peptides in the digested complex were acquired by nano-liquid chromatography Fourier transform-ion cyclotron resonance mass spectrometry (MS). The conditions of the electrospray ion s...

journal_title：Proteomics

authors： Nakamura T,Dohmae N,Takio K

更新日期：2004-09-01 00:00:00

abstract：:Heat shock proteins (HSP) are families of highly conserved proteins which are induced in cells and tissues upon exposure to extreme conditions causing acute or chronic stress. They exhibit distinct functions and have been implicated in the pathogenesis of a number of diseases, including cancer. A causal relationship b...

journal_title：Proteomics

authors： Lichtenfels R,Kellner R,Bukur J,Beck J,Brenner W,Ackermann A,Seliger B

更新日期：2002-05-01 00:00:00

abstract：:The skin provides protection against environmental stress. However, intrinsic and extrinsic aging causes significant alteration to skin structure and components, which subsequently impairs molecular characteristics and biochemical processes. Here, we have conducted an immunohistological investigation and established t...

journal_title：Proteomics

authors： Fang JY,Wang PW,Huang CH,Chen MH,Wu YR,Pan TL

更新日期：2016-10-01 00:00:00

abstract：:The use of isobaric tags such as iTRAQ allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. More classical techniques such as 2-DE can offer a complimentary approach for the analysis of complex protein samples. In this study, the proteomes of...

journal_title：Proteomics

authors： Mehaffy C,Hess A,Prenni JE,Mathema B,Kreiswirth B,Dobos KM

更新日期：2010-05-01 00:00:00

abstract：:Serum is an ideal biological sample that contains an archive of information due to the presence of a variety of proteins released by diseased tissue, and serum proteomics has gained considerable interest for the disease biomarker discovery. Easy accessibility and rapid protein changes in response to disease pathogenes...

journal_title：Proteomics

authors： Ray S,Reddy PJ,Jain R,Gollapalli K,Moiyadi A,Srivastava S

更新日期：2011-06-01 00:00:00

abstract：:Among the group of lactic acid bacteria, Streptococcus thermophilus has found a wide application in industrial processes used for the manufacture of dairy products. Taking advantage of different proteome extraction and subfractionation protocols, bacterial cytosolic and membrane proteins were isolated and resolved by ...

journal_title：Proteomics

authors： Salzano AM,Arena S,Renzone G,D'Ambrosio C,Rullo R,Bruschi M,Ledda L,Maglione G,Candiano G,Ferrara L,Scaloni A

更新日期：2007-05-01 00:00:00

abstract：:Biology is increasingly dependent on large-scale analysis, such as proteomics, creating a requirement for efficient bioinformatics. Bioinformatic predictions of biological functions rely upon correctly annotated database sequences, and the presence of inaccurately annotated or otherwise poorly described sequences intr...

journal_title：Proteomics

authors： Bengtsson-Palme J,Boulund F,Edström R,Feizi A,Johnning A,Jonsson VA,Karlsson FH,Pal C,Pereira MB,Rehammar A,Sanchez J,Sanli K,Thorell K

更新日期：2016-09-01 00:00:00

abstract：:Cancer cells maintain their intracellular ROS concentrations at required levels for their survival. Changes in ROS concentrations can regulate biochemical signaling mechanisms that control cell function. It has been demonstrated that ROS regulate the cellular events through redox regulation of redox-sensitive proteins...

journal_title：Proteomics

authors： Yuan K,Liu Y,Chen HN,Zhang L,Lan J,Gao W,Dou Q,Nice EC,Huang C

更新日期：2015-01-01 00:00:00

abstract：:In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of adipocyte-secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of murine differentiated 3T3-L1 adipocytes ...

journal_title：Proteomics

authors： Laria AE,Messineo S,Arcidiacono B,Varano M,Chiefari E,Semple RK,Rocha N,Russo D,Cuda G,Gaspari M,Brunetti A,Foti DP

更新日期：2018-04-01 00:00:00

abstract：:Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...

journal_title：Proteomics

authors： Hare NJ,Chan B,Chan E,Kaufman KL,Britton WJ,Saunders BM

更新日期：2015-09-01 00:00:00

abstract：:Lysine crotonylation (Kcr) is a recently discovered post-translational modification that potentially regulates multiple biological processes. With an objective to expand the available crotonylation datasets, LC-MS/MS is performed using mouse liver samples under normal physiological conditions to obtain in vivo crotony...

journal_title：Proteomics

authors： Liu JF,Wu SF,Liu S,Sun X,Wang XM,Xu P,Chen HZ,Yang JT

更新日期：2020-10-01 00:00:00

abstract：:In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well-wat...

journal_title：Proteomics

authors： Grimplet J,Wheatley MD,Jouira HB,Deluc LG,Cramer GR,Cushman JC

更新日期：2009-05-01 00:00:00

abstract：:In prokaryotes, acyl carrier protein (ACP) is a cofactor central to a myriad of syntheses, including fatty acid and phospholipid synthesis. To fulfill its function, ACP must therefore interact with a multitude of different enzymes, which includes the thioesterase YbgC. We found a specific interaction between ACP and Y...

journal_title：Proteomics

authors： Gully D,Bouveret E

更新日期：2006-01-01 00:00:00

abstract：:Long-lived conifer trees depend on both constitutive and induced defenses for resistance against a myriad of potential pathogens and herbivores. In species of spruce (Picea spp.), several of the late events of pathogen-, insect-, or elicitor-induced defense responses have previously been characterized at the anatomica...

journal_title：Proteomics

authors： Lippert DN,Ralph SG,Phillips M,White R,Smith D,Hardie D,Gershenzon J,Ritland K,Borchers CH,Bohlmann J

更新日期：2009-01-01 00:00:00

abstract：:Aluminum (Al) toxicity is a major constraint for plant root development and growth as well as crop yield in acidic soils, which constitute approximately 40% of the potentially arable lands worldwide. The mechanisms of Al tolerance in plants are not well understood. As a whole systems approach, proteomic techniques hav...

journal_title：Proteomics

authors： Zheng L,Lan P,Shen RF,Li WF

更新日期：2014-03-01 00:00:00

abstract：:T helper cells (Th) are divided into Th1 and Th2 subsets based upon their cytokine profiles and function. Naïve Th cells differentiate into Th1 and Th2 subsets depending on the antigens, costimulatory molecules, and cytokines they encounter. Cytokine interleukin (IL)-12 enhances the generation of Th1 lymphocytes and i...

journal_title：Proteomics

authors： Filén JJ,Nyman TA,Korhonen J,Goodlett DR,Lahesmaa R

更新日期：2005-12-01 00:00:00

abstract：:Top-down proteomics have recently started to gain attention as a novel method to provide insight into the structure of proteins in their native state, specifically the number and location of disulfide bridges. However, previous techniques still relied on complex and time-consuming protein purification and reduction re...

journal_title：Proteomics

authors： Khairallah RJ,Sadayappan S

更新日期：2013-11-01 00:00:00

abstract：:Leukaemic transformation is frequently associated with the aberrant activity of a protein tyrosine kinase (PTK). As such it is of clinical relevance to be able to map the effects of these leukaemogenic PTKs on haemopoietic cells at the level of phosphorylation modulation. In this paradigm study we have employed a rang...

journal_title：Proteomics

authors： Lee CF,Griffiths S,Rodríguez-Suárez E,Pierce A,Unwin RD,Jaworska E,Evans CA,J Gaskell S,Whetton AD

更新日期：2010-09-01 00:00:00

abstract：:The 2-D peptide separations employing mixed mode reversed phase anion exchange (MM (RP-AX)) HPLC in the first dimension in conjunction with RP chromatography in the second dimension were developed and utilised for shotgun proteome analysis. Compared with strong cation exchange (SCX) typically employed for shotgun prot...

journal_title：Proteomics

authors： Phillips HL,Williamson JC,van Elburg KA,Snijders AP,Wright PC,Dickman MJ

更新日期：2010-08-01 00:00:00

abstract：:RADARS, a rapid, automated, data archiving and retrieval software system for high-throughput proteomic mass spectral data processing and storage, is described. The majority of mass spectrometer data files are compatible with RADARS, for consistent processing. The system automatically takes unprocessed data files, iden...

journal_title：Proteomics

authors： Field HI,Fenyö D,Beavis RC

更新日期：2002-01-01 00:00:00

abstract：:Current knowledge of the physiology and phylogeny of polycyclic aromatic hydrocarbon (PAH) degrading bacteria often relies on laboratory enrichments and isolations. In the present study, in situ microcosms consisting of activated carbon pellets (BACTRAP®s) were loaded with either (13) C-naphthalene or (13) C-fluorene ...

journal_title：Proteomics

authors： Herbst FA,Bahr A,Duarte M,Pieper DH,Richnow HH,von Bergen M,Seifert J,Bombach P

更新日期：2013-10-01 00:00:00

abstract：:Proteolytic digestion is an essential step in proteomic sample processing. While this step has traditionally been implemented in homogeneous (solution) format, there is a growing trend to use heterogeneous systems in which the enzyme is immobilized on hydrogels or other solid supports. Here, we introduce the use of im...

journal_title：Proteomics

authors： Luk VN,Fiddes LK,Luk VM,Kumacheva E,Wheeler AR

更新日期：2012-05-01 00:00:00

abstract：:The soluble fraction of nuclear proteins is a functionally significant fraction, since it has been shown that it contains ribonucleoproteins active in nuclear RNA metabolism. The aim of this work was to detect variations associated with cell proliferation, by comparing two-dimensional proteomes obtained from the solub...

journal_title：Proteomics

authors： González-Camacho F,Medina FJ

更新日期：2004-02-01 00:00:00

abstract：:The E7 protein produced by high-risk human papillomavirus (HPV) induces a degradation of the retinoblastoma tumor suppressor RB through direct interaction, which suggests that an inhibitor for the interaction can be a potential anticancer drug. A surface plasmon resonance (SPR) imaging-based protein array chip was dev...

journal_title：Proteomics

authors： Jung SO,Ro HS,Kho BH,Shin YB,Kim MG,Chung BH

更新日期：2005-11-01 00:00:00

abstract：:Familial hemiplegic migraine type 1 (FHM1) is caused by missense mutations in the CACNA1A gene that encodes the alpha1A pore-forming subunit of Ca(V)2.1 Ca(2+) channels. Knock-in (KI) transgenic mice expressing Ca(V)2.1 Ca(2+) channels with a human pathogenic FHM1 mutation reveal enhanced glutamatergic neurotransmissi...

journal_title：Proteomics

authors： Klychnikov OI,Li KW,Sidorov IA,Loos M,Spijker S,Broos LA,Frants RR,Ferrari MD,Mayboroda OA,Deelder AM,Smit AB,van den Maagdenberg AM

更新日期：2010-07-01 00:00:00

abstract：:ESAT-6 (the 6 kDa early secreted antigenic target) protein species in short-term culture filtrate of Mycobacterium tuberculosis were separated in a 4-5 narrow range pI gradient two-dimensional gel electrophoresis (2-DE). Eight ESAT-6 protein species were analyzed in detail by peptide mass fingerprinting matrix-assiste...

journal_title：Proteomics

authors： Okkels LM,Müller EC,Schmid M,Rosenkrands I,Kaufmann SH,Andersen P,Jungblut PR

更新日期：2004-10-01 00:00:00

abstract：:The lipid droplet (LD) is an organelle with vital functions found in nearly all organisms. LD proteomic research has provided fundamentally important insights into this organelle's functions. The review provides a summary of LD proteomic studies conducted across diverse organisms and cell and tissue types. The accumul...

journal_title：Proteomics

authors： Zhang C,Liu P

更新日期：2019-05-01 00:00:00