RADARS, a bioinformatics solution that automates proteome mass spectral analysis, optimises protein identification, and archives data in a relational database.

abstract：:A method is introduced to evaluate protein concentrations using the height sum of all MALDI-MS peaks that unambiguously match theoretic tryptic peptide masses of the protein sought after. The method uses native chromatographic protein fractionation prior to digestion but does not require any depletion, labeling, deriv...

journal_title：Proteomics

authors： Bublitz R,Kreusch S,Ditze G,Schulze M,Cumme GA,Fischer C,Winter A,Hoppe H,Rhode H

更新日期：2006-07-01 00:00:00

abstract：:Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...

journal_title：Proteomics

authors： Kim SY,Joglekar MV,Hardikar AA,Phan TH,Khanal D,Tharkar P,Limantoro C,Johnson J,Kalionis B,Chrzanowski W

更新日期：2019-09-01 00:00:00

abstract：:In this work, for the first time, a novel C60-functionalized magnetic silica microsphere (designated C60-f-MS) was synthesized by radical polymerization of C60 molecules on the surface of magnetic silica microspheres. The resulting C60-f-MS microsphere has magnetite core and thin C60 modified silica shell, which endow...

journal_title：Proteomics

authors： Chen H,Qi D,Deng C,Yang P,Zhang X

更新日期：2009-01-01 00:00:00

abstract：:Over the last decade, an increasing prevalence of peanut allergies was observed worldwide. Peanuts are meanwhile categorized among the most dangerous food allergens. This is particularly relevant since peanut-derived ingredients are widely used in industrial food production. To minimize the problem of hidden food alle...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Latendorf T,Nebendahl M,Petersen A,Krause S,Leippe M,Becker WM,Janssen O

更新日期：2009-07-01 00:00:00

abstract：:Changing smooth muscle phenotype and abnormal cell proliferation are important features of vascular pathology, including the failure of saphenous vein bypass grafts. We have characterised and mapped protein expression in human saphenous vein medial smooth muscle, using two-dimensional (2-D) polyacrylamide gel electrop...

journal_title：Proteomics

authors： McGregor E,Kempster L,Wait R,Welson SY,Gosling M,Dunn MJ,Powel JT

更新日期：2001-11-01 00:00:00

abstract：:Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and sub-tropical oceans. It is unique in that it exclusively fixes N(2) at daytime, although it belongs to the non-heterocystous filamentous segment of the...

journal_title：Proteomics

authors： Sandh G,Ran L,Xu L,Sundqvist G,Bulone V,Bergman B

更新日期：2011-02-01 00:00:00

abstract：:Imbalance in protein homeostasis in specific subcellular organelles is alleviated through organelle-specific stress response pathways. As a canonical example of stress activated pathway, accumulation of misfolded proteins in ER activates unfolded protein response (UPR) in almost all eukaryotic organisms. However, very...

journal_title：Proteomics

authors： Maity S,Basak T,Bhat A,Bhasin N,Ghosh A,Chakraborty K,Sengupta S

更新日期：2014-08-01 00:00:00

abstract：:Simple protein separation by 1DE is a widely used method to reduce sample complexity and to prepare proteins for mass spectrometric identification via in-gel digestion. While several automated solutions are available for in-gel digestion particularly of small cylindric gel plugs derived from 2D gels, the processing of...

journal_title：Proteomics

authors： Schmidt C,Hesse D,Raabe M,Urlaub H,Jahn O

更新日期：2013-05-01 00:00:00

abstract：:The gut epithelium formed between an organism and the environment plays an essential role in host-microbe interactions, yet remains one of the least characterized mammalian tissues. Especially the membrane proteins, which are critical to bacterial adhesion, are understudied, because these proteins are low in abundance...

journal_title：Proteomics

authors： Hesselager MO,Codrea MC,Bendixen E

更新日期：2015-07-01 00:00:00

abstract：:The lipid droplet (LD) is an organelle with vital functions found in nearly all organisms. LD proteomic research has provided fundamentally important insights into this organelle's functions. The review provides a summary of LD proteomic studies conducted across diverse organisms and cell and tissue types. The accumul...

journal_title：Proteomics

authors： Zhang C,Liu P

更新日期：2019-05-01 00:00:00

abstract：:With an emphasis on obtaining a multitude of high quality tandem mass spectrometry spectra for protein identification, instrumental parameters are described for the liquid chromatography-tandem mass spectrometry analysis of trypsin digested unfractionated urine using a hybrid quadrupole-time-of-flight (Q-TOF) mass spe...

journal_title：Proteomics

authors： Davis MT,Spahr CS,McGinley MD,Robinson JH,Bures EJ,Beierle J,Mort J,Yu W,Luethy R,Patterson SD

更新日期：2001-01-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is the second cause of cancer-related deaths worldwide. A clearer understanding of the molecular mechanisms underlying tumor growth and invasiveness remains crucial for developing new therapies. Here, the expression of tetraspanins, a family of plasma membrane organizers involved in tumo...

journal_title：Proteomics

authors： Sidahmed-Adrar N,Ottavi JF,Benzoubir N,Ait Saadi T,Bou Saleh M,Mauduit P,Guettier C,Desterke C,Le Naour F

更新日期：2019-11-01 00:00:00

abstract：:To implement the 2-DE database of serogroup A Neisseria meningitidis (MenA) and improve its potential of investigation in bacterial biology, cell extracts were separated by tricine-SDS-PAGE and 131 novel proteins were identified by microLC-ESI-IT-MS/MS. These identifications extended to 404, the number of MenA gene ex...

journal_title：Proteomics

authors： Bernardini G,Arena S,Braconi D,Scaloni A,Santucci A

更新日期：2007-09-01 00:00:00

abstract：:Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...

journal_title：Proteomics

authors： Chaker S,Kashat L,Voisin S,Kaur J,Kak I,MacMillan C,Ozcelik H,Siu KW,Ralhan R,Walfish PG

更新日期：2013-03-01 00:00:00

abstract：:The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse live...

journal_title：Proteomics

authors： Peng L,Kapp EA,Fenyö D,Kwon MS,Jiang P,Wu S,Jiang Y,Aguilar MI,Ahmed N,Baker MS,Cai Z,Chen YJ,Van Chi P,Chung MC,He F,Len AC,Liao PC,Nakamura K,Ngai SM,Paik YK,Pan TL,Poon TC,Salekdeh GH,Simpson RJ,Sirdesh

更新日期：2010-11-01 00:00:00

abstract：:Cardiac conduction defects were found in transgenic mice deficient in urea transporter UT-B. To investigate the molecular mechanisms of the conduction defects caused by UT-B deletion, we studied the protein expression profiles of heart tissue (comprising most conduction system) in wild-type versus UT-B null mice at di...

journal_title：Proteomics

authors： Yu H,Meng Y,Wang LS,Jin X,Gao LF,Zhou L,Ji K,Li Y,Zhao LJ,Chen GQ,Zhao XJ,Yang B

更新日期：2009-02-01 00:00:00

abstract：:Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/cGMP (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecul...

journal_title：Proteomics

authors： Zhang G,Wong YH,Zhang Y,He LS,Xu Y,Qian PY

更新日期：2015-11-01 00:00:00

abstract：:Intrinsic or acquired resistance to vincristine (VCR), an antimicrotubule agent used in the treatment of childhood acute lymphoblastic leukemia (ALL), is a major clinical problem. Using a clinically relevant NOD/SCID mouse xenograft model of ALL, we established that alterations in the actin and tubulin cytoskeleton ar...

journal_title：Proteomics

authors： Verrills NM,Liem NL,Liaw TY,Hood BD,Lock RB,Kavallaris M

更新日期：2006-03-01 00:00:00

abstract：:Antibody microarrays have great potential for significant value in biological research. Cancer research in particular could benefit from the unique experimental capabilities of this technology. This article examines the current state of antibody microarray technological developments and assay formats, along with a rev...

journal_title：Proteomics

authors： Haab BB

更新日期：2003-11-01 00:00:00

abstract：:The hair follicle dermal papilla is composed primarily of extracellular matrix (ECM) proteins secreted by resident fibroblasts. Dermal papilla is endowed with hair morphogenic properties, yet its composition is poorly characterized. In an attempt to understand its specificity better, we compared the protein compositio...

journal_title：Proteomics

authors： Pflieger D,Chabane S,Gaillard O,Bernard BA,Ducoroy P,Rossier J,Vinh J

更新日期：2006-11-01 00:00:00

abstract：:This paper proposes a novel profiling method for SELDI-TOF and MALDI-TOF MS data that integrates a novel peak detection method based on modified smoothed non-linear energy operator, correlation-based peak selection and Bayesian additive regression trees. The peak detection and classification performance of the propose...

journal_title：Proteomics

authors： He S,Li X,Viant MR,Yao X

更新日期：2009-09-01 00:00:00

abstract：:Advances in gel-based nonradioactive protein expression and PTM detection using fluorophores has served as the impetus for developing analytical instrumentation with improved imaging capabilities. We describe a CCD camera-based imaging instrument, equipped with both a high-pressure Xenon arc lamp and a UV transillumin...

journal_title：Proteomics

authors： Scrivener E,Boghigian BA,Golenko E,Bogdanova A,Jackson P,Mikulskis A,Denoyer E,Courtney P,Lopez MF,Patton WF

更新日期：2005-11-01 00:00:00

abstract：:Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized ...

journal_title：Proteomics

authors： Holzlechner M,Reitschmidt S,Gruber S,Zeilinger S,Marchetti-Deschmann M

更新日期：2016-06-01 00:00:00

abstract：:The existence of a gamma-glutamyl cycle consisting of intracellular GSH synthesis, extrusion to the apoplastic space and recovery by gamma-glutamyl transferase (GGT)-assisted degradation into its constituent amino acids, has been demonstrated in plants. To address the significance of this cycle in plant cells, we perf...

journal_title：Proteomics

authors： Tolin S,Arrigoni G,Trentin AR,Veljovic-Jovanovic S,Pivato M,Zechman B,Masi A

更新日期：2013-06-01 00:00:00

abstract：:Protein ubiquitination is a powerful modulator of cellular functions. Classically linked to the degradation of proteins, it also plays a role in intracellular localization, DNA damage response, vesicle fusion events, and the immune and transcriptional responses. Ubiquitin is versatile and can code for several distinct...

journal_title：Proteomics

authors： Beaudette P,Popp O,Dittmar G

更新日期：2016-01-01 00:00:00

abstract：:Sample preparation is a critical step in the proteomic workflow. Numerous different approaches are used, tailored to the type of sample, the aims of the experiment, analytical method, and to an extent, user preference. This has resulted in large variation in reported protein abundances. In this study, the complementar...

journal_title：Proteomics

authors： Howard M,Achour B,Al-Majdoub Z,Rostami-Hodjegan A,Barber J

更新日期：2018-12-01 00:00:00

abstract：:Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +8...

journal_title：Proteomics

authors： Grozav AG,Willard BB,Kozuki T,Chikamori K,Micluta MA,Petrescu AJ,Kinter M,Ganapathi R,Ganapathi MK

更新日期：2011-03-01 00:00:00

abstract：:High-density lipoprotein (HDL) is the most abundant lipoprotein particle in the plasma and a negative risk factor of atherosclerosis. By using a proteomic approach it is possible to obtain detailed information about its protein content and protein modifications that may give new information about the physiological rol...

journal_title：Proteomics

authors： Karlsson H,Leanderson P,Tagesson C,Lindahl M

更新日期：2005-04-01 00:00:00

abstract：:A low virulent Candida albicans mutant, CNC13, deleted in the Mitogen Activated Protein (MAP) kynase HOG1 was used to immunize BALB/c mice. Hog1p is essential for the oxidative stress and hyperosmolarity responses. Several doses and immunization procedures were employed. The protection capacity of the different sera g...

journal_title：Proteomics

authors： Fernández-Arenas E,Molero G,Nombela C,Diez-Orejas R,Gil C

更新日期：2004-04-01 00:00:00

abstract：:Quantitative proteomic studies are contributing greatly to the understanding of the spermatozoon through the provision of detailed information on the proteins spermatozoa acquire and shed in the acquisition of fertility. Extracellular vesicles (EVs) are thought to aid in the delivery of proteins to spermatozoa in the ...

journal_title：Proteomics

authors： Leahy T,Rickard JP,Pini T,Gadella BM,de Graaf SP

更新日期：2020-06-01 00:00:00