Nitric oxide inhibits larval settlement in Amphibalanus amphitrite cyprids by repressing muscle locomotion and molting.


:Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/cGMP (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecular mechanism, we analyzed changes in the proteome of A. amphitrite cyprids in response to different concentrations of the NO donor sodium nitroprusside (SNP; 62.5, 250, and 1000 μM) using a label-free proteomics method. Compared with the control, the expression of 106 proteins differed in all three treatments. These differentially expressed proteins were assigned to 13 pathways based on KEGG pathway enrichment analysis. SNP treatment stimulated the expression of heat shock proteins and arginine kinase, which are functionally related to NO synthases, increased the expression levels of glutathione transferases for detoxification, and activated the iron-mediated fatty acid degradation pathway and the citrate cycle through ferritin. Moreover, NO repressed the level of myosins and cuticular proteins, which indicated that NO might inhibit larval settlement in A. amphitrite by modulating the process of muscle locomotion and molting.






Zhang G,Wong YH,Zhang Y,He LS,Xu Y,Qian PY




Has Abstract


2015-11-01 00:00:00












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    abstract::Trichomonas vaginalis, a sexually transmitted parasite, has many cysteine proteinases (CPs); some are involved in trichomonal pathogenesis, express during infection, and antibodies against CPs have been detected in patient sera. The goal of this study was to identify the antigenic proteinases of T. vaginalis as potent...


    pub_type: 杂志文章


    authors: Ramón-Luing LA,Rendón-Gandarilla FJ,Cárdenas-Guerra RE,Rodríguez-Cabrera NA,Ortega-López J,Avila-González L,Angel-Ortiz C,Herrera-Sánchez CN,Mendoza-García M,Arroyo R

    更新日期:2010-02-01 00:00:00

  • Identification of specific plant nucleolar phosphoproteins in a functional proteomic analysis.

    abstract::The soluble fraction of nuclear proteins is a functionally significant fraction, since it has been shown that it contains ribonucleoproteins active in nuclear RNA metabolism. The aim of this work was to detect variations associated with cell proliferation, by comparing two-dimensional proteomes obtained from the solub...


    pub_type: 杂志文章


    authors: González-Camacho F,Medina FJ

    更新日期:2004-02-01 00:00:00

  • Comprehensive proteome profiling of the Fe(III)-reducing myxobacterium Anaeromyxobacter dehalogenans 2CP-C during growth with fumarate and ferric citrate.

    abstract::Anaeromyxobacter dehalogenans is a microaerophilic member of the delta-proteobacteria which is able to utilize a wide range of electron acceptors, including halogenated phenols, U(VI), Fe(III), nitrate, nitrite, oxygen and fumarate. To date, the knowledge regarding general metabolic activities of this ecologically rel...


    pub_type: 杂志文章


    authors: Chao TC,Kalinowski J,Nyalwidhe J,Hansmeier N

    更新日期:2010-04-01 00:00:00

  • Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis.

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    authors: Eymann C,Becher D,Bernhardt J,Gronau K,Klutzny A,Hecker M

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  • Study of rat hypothalamic proteome by HPLC/ESI ion trap and HPLC/ESI-Q-TOF MS.

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    pub_type: 杂志文章


    authors: Iqbal J,Li W,Ullah K,Hasan M,Linna G,Awan U,Zhang Y,Batool S,Qing H,Deng Y

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  • A widespread picture of the Streptococcus thermophilus proteome by cell lysate fractionation and gel-based/gel-free approaches.

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    authors: Salzano AM,Arena S,Renzone G,D'Ambrosio C,Rullo R,Bruschi M,Ledda L,Maglione G,Candiano G,Ferrara L,Scaloni A

    更新日期:2007-05-01 00:00:00

  • Differential expression profile of membrane proteins in zebrafish (Danio rerio) brain exposed to methyl parathion.

    abstract::Methyl parathion (MP) is a widely used organophosphorus pesticide, which has been related to a broad spectrum of toxic effects on environmental organisms. The present study investigated the changes in the protein profile of enriched membrane fraction from zebrafish (Danio rerio) brain exposed to three concentrations (...


    pub_type: 杂志文章


    authors: Huang QY,Huang HQ

    更新日期:2011-09-01 00:00:00

  • Experimental annotation of channel catfish virus by probabilistic proteogenomic mapping.

    abstract::Experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. Channel catfish herpesvirus (CCV) is a simple comparative model for understanding herp...


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    authors: Kunec D,Nanduri B,Burgess SC

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  • Protein profiling of epidermal bladder cells from the halophyte Mesembryanthemum crystallinum.

    abstract::Plant epidermal trichomes are as varied in morphology as they are in function. In the halophyte Mesembryanthemum crystallinum, specialized trichomes called epidermal bladder cells (EBC) line the surface of leaves and stems, and increase dramatically in size and volume upon plant salt-treatment. These cells have been p...


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    authors: Barkla BJ,Vera-Estrella R,Pantoja O

    更新日期:2012-09-01 00:00:00

  • The methylproteome and the intracellular methylation network.

    abstract::Since its discovery more than 50 years ago, post-translational modification (PTM) of proteins via methylation has grown in prominence, its involvement having been recognised in a number of central processes in the cell. Of these, the best characterised is its role in the epigenetic code. However, there is increasing e...


    pub_type: 杂志文章,评审


    authors: Erce MA,Pang CN,Hart-Smith G,Wilkins MR

    更新日期:2012-02-01 00:00:00

  • Proteome analysis of chick embryonic cerebrospinal fluid.

    abstract::During early stages of embryo development, the brain cavity is filled with embryonic cerebrospinal fluid (E-CSF), a complex fluid containing different protein fractions that contributes to the regulation of the survival, proliferation and neurogenesis of the neuroectodermal stem cells. Using 2-DE, protein sequencing a...


    pub_type: 杂志文章


    authors: Parada C,Gato A,Aparicio M,Bueno D

    更新日期:2006-01-01 00:00:00

  • Mass spectrometry-based immuno-precipitation proteomics - the user's guide.

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    authors: ten Have S,Boulon S,Ahmad Y,Lamond AI

    更新日期:2011-03-01 00:00:00

  • The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard.

    abstract::The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse live...


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    authors: Peng L,Kapp EA,Fenyö D,Kwon MS,Jiang P,Wu S,Jiang Y,Aguilar MI,Ahmed N,Baker MS,Cai Z,Chen YJ,Van Chi P,Chung MC,He F,Len AC,Liao PC,Nakamura K,Ngai SM,Paik YK,Pan TL,Poon TC,Salekdeh GH,Simpson RJ,Sirdesh

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  • Proteomics indicates modulation of tubulin polymerization by L-menthol inhibiting human epithelial colorectal adenocarcinoma cell proliferation.

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    authors: Faridi U,Sisodia BS,Shukla AK,Shukla RK,Darokar MP,Dwivedi UN,Shasany AK

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    authors: da Silva AJ,Gómez-Mendoza DP,Junqueira M,Domont GB,Ximenes Ferreira Filho E,de Sousa MV,Ricart CA

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    authors: Hendriks MM,Smit S,Akkermans WL,Reijmers TH,Eilers PH,Hoefsloot HC,Rubingh CM,de Koster CG,Aerts JM,Smilde AK

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  • Purification and proteomic analysis of outer membrane vesicles from a clinical isolate of Leptospira interrogans serovar Copenhageni.

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    authors: Nally JE,Whitelegge JP,Aguilera R,Pereira MM,Blanco DR,Lovett MA

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  • The phosphate-starvation response in Vibrio cholerae O1 and phoB mutant under proteomic analysis: disclosing functions involved in adaptation, survival and virulence.

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    authors: von Krüger WM,Lery LM,Soares MR,de Neves-Manta FS,Batista e Silva CM,Neves-Ferreira AG,Perales J,Bisch PM

    更新日期:2006-03-01 00:00:00

  • Secretome proteins as candidate biomarkers for aggressive thyroid carcinomas.

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    pub_type: 杂志文章


    authors: Chaker S,Kashat L,Voisin S,Kaur J,Kak I,MacMillan C,Ozcelik H,Siu KW,Ralhan R,Walfish PG

    更新日期:2013-03-01 00:00:00

  • Requirements for the valid quantification of immunostains on tissue microarray materials using image analysis.

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    pub_type: 杂志文章


    authors: Decaestecker C,Lopez XM,D'Haene N,Roland I,Guendouz S,Duponchelle C,Berton A,Debeir O,Salmon I

    更新日期:2009-10-01 00:00:00

  • Proteomic analysis in human breast cancer: identification of a characteristic protein expression profile of malignant breast epithelium.

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    pub_type: 杂志文章


    authors: Hudelist G,Singer CF,Pischinger KI,Kaserer K,Manavi M,Kubista E,Czerwenka KF

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    abstract::Proteins undergo PTM, which modulates their structure and regulates their function. Estimates of the PTM occurrence vary but it is safe to assume that there is an important gap between what is currently known and what remains to be discovered. The highest throughput and most comprehensive efforts to catalogue protein ...


    pub_type: 杂志文章


    authors: Ahrné E,Müller M,Lisacek F

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    pub_type: 杂志文章,评审


    authors: Ruhen O,Meehan K

    更新日期:2019-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Ahn DH,Singaravelu G,Lee S,Ahnn J,Shim YH

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    pub_type: 杂志文章


    authors: Holtappels M,Noben JP,Valcke R

    更新日期:2016-09-01 00:00:00

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    authors: Karp NA,Lilley KS

    更新日期:2007-09-01 00:00:00

  • The Origins of Organellar Mapping by Protein Correlation Profiling.

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    pub_type: 杂志文章


    authors: Mann M

    更新日期:2020-08-03 00:00:00

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    abstract::White spot syndrome virus (WSSV) is a pathogen that causes considerable mortality of the farmed shrimp, Penaeus monodon. Candidate 'vaccines', WSSV envelope protein VP28 and formalin-inactivated WSSV, can provide short-lived protection against the virus. In this study, P. monodon was orally intubated with the aforemen...


    pub_type: 杂志文章


    authors: Kulkarni AD,Kiron V,Rombout JH,Brinchmann MF,Fernandes JM,Sudheer NS,Singh BI

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