Abstract:
:Nitric oxide (NO) is a universal signaling molecule and plays a negative role in the metamorphosis of many biphasic organisms. Recently, the NO/cGMP (cyclic guanosine monophosphate) signaling pathway was reported to repress larval settlement in the barnacle Amphibalanus amphitrite. To understand the underlying molecular mechanism, we analyzed changes in the proteome of A. amphitrite cyprids in response to different concentrations of the NO donor sodium nitroprusside (SNP; 62.5, 250, and 1000 μM) using a label-free proteomics method. Compared with the control, the expression of 106 proteins differed in all three treatments. These differentially expressed proteins were assigned to 13 pathways based on KEGG pathway enrichment analysis. SNP treatment stimulated the expression of heat shock proteins and arginine kinase, which are functionally related to NO synthases, increased the expression levels of glutathione transferases for detoxification, and activated the iron-mediated fatty acid degradation pathway and the citrate cycle through ferritin. Moreover, NO repressed the level of myosins and cuticular proteins, which indicated that NO might inhibit larval settlement in A. amphitrite by modulating the process of muscle locomotion and molting.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Zhang G,Wong YH,Zhang Y,He LS,Xu Y,Qian PYdoi
10.1002/pmic.201500112subject
Has Abstractpub_date
2015-11-01 00:00:00pages
3854-64issue
22eissn
1615-9853issn
1615-9861journal_volume
15pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::The discovery of PTMs in proteins by MS requires nearly complete sequence coverage of the detected proteolytic peptides. Unfortunately, mass spectrometric analysis of the desired sequence fragments is often impeded due to low ionization efficiency and/or signal suppression in complex samples. When several lysine resid...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300309
更新日期:2014-03-01 00:00:00
abstract::SARS-CoV-2 infection results in a mild-to-moderate disease course in most patients, allowing outpatient self-care and quarantine. However, in approx. 10% of cases a two- or three-phasic critical disease course with starting from day 7 to 10 is observed. To facilitate and plan outpatient care, biomarkers prognosing suc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000202
更新日期:2020-09-10 00:00:00
abstract::The rice heterotrimeric G-protein complex, a guanine-nucleotide-dependent on-off switch, mediates vital cellular processes and responses to biotic and abiotic stress. Exchange of bound GDP (resting state) for GTP (active state) is spontaneous in plants including rice and thus there is no need for promoting guanine nuc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800385
更新日期:2019-05-01 00:00:00
abstract::Many types of cells transit in vitro from a two- to a three-dimensional growth, when they are exposed to microgravity. The underlying mechanisms are not yet understood. Hence, we investigated the impact of microgravity on protein content and growth behavior. For this purpose, the human thyroid cancer cells FTC-133 wer...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500067
更新日期:2015-09-01 00:00:00
abstract::The cancer tissue proteome has enormous potential as a source of novel predictive biomarkers in oncology. Progress in the development of mass spectrometry (MS)-based tissue proteomics now presents an opportunity to exploit this by applying the strategies of comprehensive molecular profiling and big-data analytics that...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900109
更新日期:2019-11-01 00:00:00
abstract::In this study, a method, based on dual column affinity chromatography hyphenated to isotope dilution inductively coupled plasma-quadrupole MS, was developed for selenium determination in selenoprotein P, glutathione peroxidase, and selenoalbumin in human serum samples from a group of healthy volunteers (n=399). Method...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900677
更新日期:2010-10-01 00:00:00
abstract::3Z-3-[(1H-pyrrol-2-yl)-methylidene]-1-(1-piperidinylmethyl)-1,3-2H-indol-2-one (Z24), a synthetic anti-angiogenic compound, inhibits the growth and metastasis of certain tumors. Previous works have shown that Z24 induces hepatotoxicity in rodents. We examined the hepatotoxic mechanism of Z24 at the protein level and l...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900699
更新日期:2010-08-01 00:00:00
abstract::A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000218
更新日期:2021-01-01 00:00:00
abstract::MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600682
更新日期:2007-02-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) causes an acute, highly contagious, and devastating viral enteric disease with a high mortality rate in suckling pigs. A large-scale outbreak of PED occurred in China in 2010, with PEDV emerging in the United States in 2013 and spreading rapidly, posing significant economic and p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400458
更新日期:2015-06-01 00:00:00
abstract::Top-down proteomics have recently started to gain attention as a novel method to provide insight into the structure of proteins in their native state, specifically the number and location of disulfide bridges. However, previous techniques still relied on complex and time-consuming protein purification and reduction re...
journal_title:Proteomics
pub_type: 评论,杂志文章
doi:10.1002/pmic.201300445
更新日期:2013-11-01 00:00:00
abstract::Gan et al. (Proteomics 2013, 13, 3117-3123) described a new "macropore" protocol for effective protein digestion by trypsin suitable for a wide range of pH including acidic pH. It was effective not only in experiments with solutions of a model protein (myoglobin), but also with a subfraction of rat liver cytosol. This...
journal_title:Proteomics
pub_type: 评论,杂志文章
doi:10.1002/pmic.201300440
更新日期:2013-11-01 00:00:00
abstract::Malt derived from barley malting is an essential raw material for beer brewing. In this study, we performed the first dynamic proteome survey during barley malting using a gel-free proteomics approach. This entailed in-solution tryptic digestion of precipitated proteins and analysis of peptides by nanoliquid chromatog...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800025
更新日期:2018-08-01 00:00:00
abstract::The explosion of site- and context-specific in vivo phosphorylation events presents a potentially rich source of biological knowledge and calls for novel data analysis and modeling paradigms. Perhaps the most immediate challenge is delineating detected phosphorylation sites to their effector kinases. This is important...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200900266
更新日期:2009-12-01 00:00:00
abstract::Of all protein PTMs, glycosylation is by far the most common, and is a target for proteomic research. Glycosylation plays key roles in controlling various cellular processes and the modifications of the glycan structures in diseases highlight the clinical importance of this PTM. Glycosylation analysis remains a diffic...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200600129
更新日期:2006-07-01 00:00:00
abstract::Myelination of the CNS is performed by oligodendrocytes (OLs), which have been implicated in brain disorders, such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an OL reference proteome database. Proteins were prefractionationated by SDS-PAGE and after i...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300201
更新日期:2013-12-01 00:00:00
abstract::Two-dimensional difference gel electrophoresis (2-D DIGE) allows for reliable quantification of global protein abundance changes. The threshold of significance for protein abundance changes depends on the experimental variation (biological and technical). This study estimates biological, technical and total variation ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100071
更新日期:2011-08-01 00:00:00
abstract::High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effec...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200402086
更新日期:2006-03-01 00:00:00
abstract::Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500510
更新日期:2016-06-01 00:00:00
abstract::Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +8...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000194
更新日期:2011-03-01 00:00:00
abstract::To develop a standard method for separating highly basic proteins in mammalian cells, we established a 2-D LC separation system coupled with chromatofocusing/nonporous RP column chromatography (CF/NPRPC) in a ProteomeLab PF2D system. After standardizing conditions for 2-D LC, a 2-D liquid protein map of uninfected mac...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500433
更新日期:2006-02-01 00:00:00
abstract::Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000316
更新日期:2010-11-01 00:00:00
abstract::The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200800138
更新日期:2008-06-01 00:00:00
abstract::Protein complexes carry out almost the entire signaling and functional processes in the cell. The protein complex complement of a cell, and its network of complex-complex interactions, is referred to here as the complexome. Computational methods to predict protein complexes from proteomics data, resulting in network r...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201300230
更新日期:2014-01-01 00:00:00
abstract::Proteolytic digestion is an essential step in proteomic sample processing. While this step has traditionally been implemented in homogeneous (solution) format, there is a growing trend to use heterogeneous systems in which the enzyme is immobilized on hydrogels or other solid supports. Here, we introduce the use of im...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100608
更新日期:2012-05-01 00:00:00
abstract::The ZNF198-fibroblast growth factor receptor-1 (FGFR1) fusion kinase is a constitutively activated tyrosine kinase associated with a specific atypical myeloproliferative disease. The chimeric protein localizes to the cytoplasm, unlike the wild type FGFR1 receptor kinase, and presumably inappropriately phosphorylates s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800852
更新日期:2009-08-01 00:00:00
abstract::The transfection of glycoprotein glycosyltransferase genes into cells leads to modification of both the structure and function of the glycoproteins and as a result, changes in glycome patterns. N-glycan branching enzymes hold some promise as a model system for the identification of glycome patterns. Both N-acetylgluco...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/1615-9861(200102)1:2<239::AID-PROT239>3.0.
更新日期:2001-02-01 00:00:00
abstract::An aim of proteome research is to identify the entire complement of proteins expressed in defined cell types of humans, animals, plants, and microorganisms. The approach requires searching for low abundant or even rarely expressed proteins in many cell types, as well as the determination of the protein expression leve...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201300253
更新日期:2014-03-01 00:00:00
abstract::In this work, for the first time, a novel C60-functionalized magnetic silica microsphere (designated C60-f-MS) was synthesized by radical polymerization of C60 molecules on the surface of magnetic silica microspheres. The resulting C60-f-MS microsphere has magnetite core and thin C60 modified silica shell, which endow...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800335
更新日期:2009-01-01 00:00:00
abstract::DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800298
更新日期:2008-12-01 00:00:00