Abstract:
:Serum is an ideal biological sample that contains an archive of information due to the presence of a variety of proteins released by diseased tissue, and serum proteomics has gained considerable interest for the disease biomarker discovery. Easy accessibility and rapid protein changes in response to disease pathogenesis makes serum an attractive sample for clinical research. Despite these advantages, the analysis of serum proteome is very challenging due to the wide dynamic range of proteins, difficulty in finding low-abundance target analytes due to the presence of high-abundance serum proteins, high levels of salts and other interfering compounds, variations among individuals and paucity of reproducibility. Sample preparation introduces pre-analytical variations and poses major challenges to analyze the serum proteome. The label-free detection techniques such as surface plasmon resonance, microcantilever, few nanotechniques and different resonators are rapidly emerging for the analysis of serum proteome and they have exhibited potential to overcome few limitations of the conventional techniques. In this article, we will discuss the current status of serum proteome analysis for the biomarker discovery and address key technological advancements, with a focus on challenges and amenable solutions.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Ray S,Reddy PJ,Jain R,Gollapalli K,Moiyadi A,Srivastava Sdoi
10.1002/pmic.201000460subject
Has Abstractpub_date
2011-06-01 00:00:00pages
2139-61issue
11eissn
1615-9853issn
1615-9861journal_volume
11pub_type
杂志文章,评审相关文献
PROTEOMICS文献大全abstract::Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000316
更新日期:2010-11-01 00:00:00
abstract::In higher plants many different genes encode Lhcb proteins that belong to a highly conserved protein family. Evolutionary conservation of this genetic redundancy suggests that individual gene products play different roles in light harvesting and photoprotection depending on environmental conditions. We have tested the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200402008
更新日期:2005-02-01 00:00:00
abstract::Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800166
更新日期:2019-09-01 00:00:00
abstract::Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic func...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800469
更新日期:2019-06-01 00:00:00
abstract::The butyrate-producing anaerobe Fusobacterium varium is an integral constituent of human gut microflora. Unlike many gut microorganisms, F. varium is capable of fermenting both amino acids and glucose. Although F. varium has been implicated in beneficial as well as pathological bacterium-host interactions, its genome ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700437
更新日期:2008-07-01 00:00:00
abstract::In this work, for the first time, perfluorinated magnetic mesoporous microspheres were designed and synthesized for the highly specific enrichment of fluorous-derivatized phosphopeptides through the unique fluorine-fluorine interactions. The perfluorinated magnetic mesoporous microspheres were prepared through a surfa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500323
更新日期:2016-04-01 00:00:00
abstract::To implement the 2-DE database of serogroup A Neisseria meningitidis (MenA) and improve its potential of investigation in bacterial biology, cell extracts were separated by tricine-SDS-PAGE and 131 novel proteins were identified by microLC-ESI-IT-MS/MS. These identifications extended to 404, the number of MenA gene ex...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700210
更新日期:2007-09-01 00:00:00
abstract::Helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. In addition H. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are a...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200390040
更新日期:2003-03-01 00:00:00
abstract::Blood plasma is the most complex human-derived proteome, containing other tissue proteomes as subsets. This proteome has only been partially characterized due to the extremely wide dynamic range of the plasma proteins of more than ten orders of magnitude. Thus, the reduction in sample complexity prior to mass spectrom...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700166
更新日期:2007-12-01 00:00:00
abstract::Anaeromyxobacter dehalogenans is a microaerophilic member of the delta-proteobacteria which is able to utilize a wide range of electron acceptors, including halogenated phenols, U(VI), Fe(III), nitrate, nitrite, oxygen and fumarate. To date, the knowledge regarding general metabolic activities of this ecologically rel...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900687
更新日期:2010-04-01 00:00:00
abstract::LTQ Orbitrap data analyzed with ProteinPilot can be further improved by MaxQuant raw data processing, which utilizes precursor-level high mass accuracy data for peak processing and MGF creation. In particular, ProteinPilot results from MaxQuant-processed peaklists for Orbitrap data sets resulted in improved spectral u...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100097
更新日期:2012-06-01 00:00:00
abstract::New insights on cellular heterogeneity in the last decade provoke the development of a variety of single cell omics tools at a lightning pace. The resultant high-dimensional single cell data generated by these tools require new theoretical approaches and analytical algorithms for effective visualization and interpreta...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201600267
更新日期:2017-02-01 00:00:00
abstract::Advanced glycation endproduct (AGE) formation is an important mechanism for protein deterioration during diabetic complications and ageing. The effects on AGE formation following dihydroxyacetone (DHA) stress were studied in two model organisms, the yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegan...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700165
更新日期:2007-10-01 00:00:00
abstract::Transmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders characterized by the accumulation in the CNS of a pathological conformer (PrP(TSE)) of the host-encoded cellular prion protein (PrP(C)). PrP(TSE) has a central role in the pathogenesis of the disease but other factors are likely involved ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900085
更新日期:2009-08-01 00:00:00
abstract::The complete sequencing of the Mycobacterium tuberculosis genome offers a unique opportunity to fully elucidate the biology of this human pathogen. One aspect of significant importance is the definition of T cell antigens. This report describes the development and implementation of a proteomic approach to defining suc...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200104)1:4<574::AID-PROT574>3.0.
更新日期:2001-04-01 00:00:00
abstract::Malt derived from barley malting is an essential raw material for beer brewing. In this study, we performed the first dynamic proteome survey during barley malting using a gel-free proteomics approach. This entailed in-solution tryptic digestion of precipitated proteins and analysis of peptides by nanoliquid chromatog...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800025
更新日期:2018-08-01 00:00:00
abstract::Transcription factors (TFs) are a family of DNA-binding proteins whose gene regulatory capabilities are of vital importance in defining the molecular state of a cell. Despite their biological significance, our understanding of TF behavior and function is still limited. This is because we have so far mostly relied on g...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201600317
更新日期:2017-02-01 00:00:00
abstract::The hepatitis B virus X-protein (HBx), a multifunctional viral regulator, participates in the viral life cycle and in the development of hepatocellular carcinoma (HCC). We previously reported a high incidence of HCC in transgenic mice expressing HBx. In this study, proteomic analysis was performed to identify proteins...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800779
更新日期:2009-11-01 00:00:00
abstract::Absolute protein concentration determination is becoming increasingly important in a number of fields including diagnostics, biomarker discovery and systems biology modeling. The recently introduced quantification concatamer methodology provides a novel approach to performing such determinations, and it has been appli...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000454
更新日期:2011-01-01 00:00:00
abstract::Abnormalities of the anterior cingulate cortex have previously been described in schizophrenia, major depressive disorder and bipolar disorder. In this study 2-DE was performed followed by mass spectrometric sequencing to identify disease-specific protein changes within the anterior cingulate cortex in these psychiatr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500069
更新日期:2006-06-01 00:00:00
abstract::The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200800138
更新日期:2008-06-01 00:00:00
abstract::Due to the limited applicability of conventional protein identification methods to the proteomes of organisms with unsequenced genomes, researchers have developed approaches to identify proteins using mass spectrometry and sequence similarity database searches. Both the integration of mass spectrometry with bioinforma...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200390004
更新日期:2003-01-01 00:00:00
abstract::The present review deals with the use of combinatorial ligand libraries, composed by hexapeptides, in the capture and concentration of the low-abundance proteome. This method, first reported in 2005, is compared with other current methodologies aimed at exploring the "deep proteome", such as: depletion of high-abundan...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800389
更新日期:2009-03-01 00:00:00
abstract::Brucella melitensis is a facultative intracellular bacterial pathogen that causes brucellosis, a zoonotic disease primarily infecting sheep and goats, characterized by undulant fever, arthritic pain and other neurological disorders in humans. A comprehensive proteomic study of strain 16M was conducted to identify and ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200208)2:8<1047::AID-PROT1047>3.
更新日期:2002-08-01 00:00:00
abstract::Long-lived conifer trees depend on both constitutive and induced defenses for resistance against a myriad of potential pathogens and herbivores. In species of spruce (Picea spp.), several of the late events of pathogen-, insect-, or elicitor-induced defense responses have previously been characterized at the anatomica...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800252
更新日期:2009-01-01 00:00:00
abstract::Shewanella inhabit a wide variety of niches in nature and can utilize a broad spectrum of electron acceptors under anaerobic conditions. How they modulate their gene expression to adapt is poorly understood. ArcA, homologue of a global regulator controlling hundreds of genes involved in aerobic and anaerobic respirati...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100651
更新日期:2012-06-01 00:00:00
abstract::We report on quality control performed in the context of a large-scale, multi-institutional study of the immune response in blood samples from prostate cancer patients. The measurements were performed by two commercially available techniques/services: protein arrays and an automated bead-based ELISA-like technique on ...
journal_title:Proteomics
pub_type: 杂志文章,多中心研究
doi:10.1002/pmic.201200082
更新日期:2012-09-01 00:00:00
abstract::The G-protein complex is a cytoplasmic on-off molecular switch that is set by plasma membrane receptors that activate upon binding of its cognate extracellular agonist. In animals, the default setting is the "off" resting state, while in plants, the default state is constitutively "on" but repressed by a plasma membra...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800323
更新日期:2018-12-01 00:00:00
abstract::To investigate the dynamic cellular response to a condition change, selective labeling of the nascent proteome is necessary. Here, we report a method combining click chemistry protein labeling with 2D DIGE. To test the relevance of the method, we compared nascent proteomes of actively growing bacterial cells with that...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200393
更新日期:2013-01-01 00:00:00
abstract::Tectona grandis L. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree select...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100183
更新日期:2012-04-01 00:00:00