On-chip microextraction for proteomic sample preparation of in-gel digests.

abstract：:The proteomic profile of hypothalamus, a key organ of CNS, is explored here by employing two widely used MS techniques, i.e. HPLC/ESI-ion trap and HPLC/ESI-quadrupole-TOF MS. Strong cation exchange is used for the fractionation of peptides and protein search engine MASCOT is employed for data query. One hundred and th...

journal_title：Proteomics

authors： Iqbal J,Li W,Ullah K,Hasan M,Linna G,Awan U,Zhang Y,Batool S,Qing H,Deng Y

更新日期：2013-08-01 00:00:00

abstract：:In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons: Low price, Visibl...

journal_title：Proteomics

authors： Westermeier R

更新日期：2006-09-01 00:00:00

abstract：:Liquid Chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS) is a powerful analytical technique for the identification and mass analysis of complex protein mixtures. Here, we present a combination of methods developed for the extensive/deep proteomic analysis of purified ribosome/mRNA particles assembled i...

journal_title：Proteomics

authors： Chicher J,Simonetti A,Kuhn L,Schaeffer L,Hammann P,Eriani G,Martin F

更新日期：2015-07-01 00:00:00

abstract：:In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...

journal_title：Proteomics

authors： Juan HF,Chen JH,Hsu WT,Huang SC,Chen ST,Yi-Chung Lin J,Chang YW,Chiang CY,Wen LL,Chan DC,Liu YC,Chen YJ

更新日期：2004-09-01 00:00:00

abstract：:Transcription factors (TFs) are a family of DNA-binding proteins whose gene regulatory capabilities are of vital importance in defining the molecular state of a cell. Despite their biological significance, our understanding of TF behavior and function is still limited. This is because we have so far mostly relied on g...

journal_title：Proteomics

authors： Simicevic J,Deplancke B

更新日期：2017-02-01 00:00:00

abstract：:Sandwich ELISA microarrays have great potential for validating disease biomarkers. Each ELISA relies on robust-affinity reagents that retain activity when immobilized on a solid surface or when labeled for detection. Single-chain antibodies (scFv) are affinity reagents that have greater potential for high-throughput p...

journal_title：Proteomics

authors： Seurynck-Servoss SL,Baird CL,Miller KD,Pefaur NB,Gonzalez RM,Apiyo DO,Engelmann HE,Srivastava S,Kagan J,Rodland KD,Zangar RC

更新日期：2008-06-01 00:00:00

abstract：:Following transmission to the vertebrate host, the protozoan parasite Leishmania donovani differentiates into the pathogenic amastigote stage that is adapted for intracellular survival. This developmental transition is induced by environmental factors including elevated temperature and acidic pH and is likely transduc...

journal_title：Proteomics

authors： Morales MA,Watanabe R,Laurent C,Lenormand P,Rousselle JC,Namane A,Späth GF

更新日期：2008-01-01 00:00:00

abstract：:In this study, various proteomics-based methods were utilized to examine the 14-3-3 protein family in Arabidopsis thaliana. A protein extract was prepared from an Arabidopsis hypocotyl suspension culture and analyzed by two-dimensional gel electrophoresis and immunoblotting with a 14-3-3 monoclonal antibody that recog...

journal_title：Proteomics

authors： Fuller B,Stevens SM Jr,Sehnke PC,Ferl RJ

更新日期：2006-05-01 00:00:00

abstract：:Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic func...

journal_title：Proteomics

authors： Guergues J,Zhang P,Liu B,Stevens SM Jr

更新日期：2019-06-01 00:00:00

abstract：:Helicobacter pylori is one of the most common bacterial pathogens and causes a variety of diseases, such as peptic ulcer or gastric cancer. Despite intensive study of this human pathogen in the last decades, knowledge about its membrane proteins and, in particular, those which are putative components of the type IV se...

journal_title：Proteomics

authors： Backert S,Kwok T,Schmid M,Selbach M,Moese S,Peek RM Jr,König W,Meyer TF,Jungblut PR

更新日期：2005-04-01 00:00:00

abstract：:A novel strain of influenza A H1N1 emerged in the spring of 2009 and has spread rapidly throughout the world. Although vaccines have recently been developed that are expected to be protective, their availability was delayed until well into the influenza season. Although anti-influenza drugs such as neuraminidase inhib...

journal_title：Proteomics

authors： Chen CH,Zhang XQ,Lo CW,Liu PF,Liu YT,Gallo RL,Hsieh MF,Schooley RT,Huang CM

更新日期：2010-06-01 00:00:00

abstract：:Microparticles (MPs) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M. tb) infection of macrophages increased the release of MPs, and these M...

journal_title：Proteomics

authors： Hare NJ,Chan B,Chan E,Kaufman KL,Britton WJ,Saunders BM

更新日期：2015-09-01 00:00:00

abstract：:O-GlcNAcylation is a dynamic PTM of nuclear and cytoplasmic proteins, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase, which catalyze the addition and removal of O-GlcNAc, respectively. This modification is associated with glucose metabolism, which plays important roles in many diseases including cancer. Altho...

journal_title：Proteomics

authors： Champattanachai V,Netsirisawan P,Chaiyawat P,Phueaouan T,Charoenwattanasatien R,Chokchaichamnankit D,Punyarit P,Srisomsap C,Svasti J

更新日期：2013-07-01 00:00:00

abstract：:Transmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders characterized by the accumulation in the CNS of a pathological conformer (PrP(TSE)) of the host-encoded cellular prion protein (PrP(C)). PrP(TSE) has a central role in the pathogenesis of the disease but other factors are likely involved ...

journal_title：Proteomics

authors： Giorgi A,Di Francesco L,Principe S,Mignogna G,Sennels L,Mancone C,Alonzi T,Sbriccoli M,De Pascalis A,Rappsilber J,Cardone F,Pocchiari M,Maras B,Schininà ME

更新日期：2009-08-01 00:00:00

abstract：:In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator(®) system, we identified 1340 unique proteins. Functional classification showed that most of these were associated ...

journal_title：Proteomics

authors： Yang X,Levin Y,Rahmoune H,Ma D,Schöffmann S,Umrania Y,Guest PC,Bahn S

更新日期：2011-02-01 00:00:00

abstract：:Advances in gel-based nonradioactive protein expression and PTM detection using fluorophores has served as the impetus for developing analytical instrumentation with improved imaging capabilities. We describe a CCD camera-based imaging instrument, equipped with both a high-pressure Xenon arc lamp and a UV transillumin...

journal_title：Proteomics

authors： Scrivener E,Boghigian BA,Golenko E,Bogdanova A,Jackson P,Mikulskis A,Denoyer E,Courtney P,Lopez MF,Patton WF

更新日期：2005-11-01 00:00:00

abstract：:Glycan-binding proteins (GBPs) play an important role in cell adhesion, bacterial/viral infection, and cellular signaling pathways. However, little is known about the precision alteration of GBPs referred to pathological changes in hepatic stellate cells (HSCs) during liver fibrosis. Here, the carbohydrate microarrays...

journal_title：Proteomics

authors： Zhong Y,Qin Y,Dang L,Jia L,Zhang Z,Wu H,Cui J,Bian H,Li Z

更新日期：2015-10-01 00:00:00

abstract：:This is the first broad proteomic description of Gluconacetobacter diazotrophicus, an endophytic bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in tropical regions. Proteomic coverage of G. diazotrophicus PAL5 was obtained by two independent approaches: 2-DE followed by MA...

journal_title：Proteomics

authors： Lery LM,Coelho A,von Kruger WM,Gonçalves MS,Santos MF,Valente RH,Santos EO,Rocha SL,Perales J,Domont GB,Teixeira KR,Bertalan M,Ferreira PC,Bisch PM

更新日期：2008-04-01 00:00:00

abstract：:Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional, immunological and structural aspects of both eukaryotic and prokaryotic glycoproteins. Traditional strategies used to analyse glycoprotein O-glycans involve glycoprotein isolation, followed by glycan release using solu...

journal_title：Proteomics

authors： Taylor AM,Holst O,Thomas-Oates J

更新日期：2006-05-01 00:00:00

abstract：:The ascomycete Botrytis cinerea is a phytopathogenic fungus infecting and causing significant yield losses in a number of crops. Moreover, in the last few years, B. cinerea has been adopted as an important model system in molecular phytopathology. In spite of these contributions, the molecular basis of the infection c...

journal_title：Proteomics

authors： Fernández-Acero FJ,Colby T,Harzen A,Cantoral JM,Schmidt J

更新日期：2009-05-01 00:00:00

abstract：:We describe the first phosphoproteome of the model filamentous fungus Aspergillus nidulans. Phosphopeptides were enriched using titanium dioxide, separated using a convenient ultra-long reverse phase gradient, and identified using a "high-high" strategy (high mass accuracy on the parent and fragment ions) with higher-...

journal_title：Proteomics

authors： Ramsubramaniam N,Harris SD,Marten MR

更新日期：2014-11-01 00:00:00

abstract：:Porcine epidemic diarrhea virus (PEDV) causes an acute, highly contagious, and devastating viral enteric disease with a high mortality rate in suckling pigs. A large-scale outbreak of PED occurred in China in 2010, with PEDV emerging in the United States in 2013 and spreading rapidly, posing significant economic and p...

journal_title：Proteomics

authors： Zeng S,Zhang H,Ding Z,Luo R,An K,Liu L,Bi J,Chen H,Xiao S,Fang L

更新日期：2015-06-01 00:00:00

abstract：:The severe pulmonary form of leptospirosis (SPFL) is an especially serious and rapid disease process characterized by alveolar hemorrhage and acute respiratory failure. The outer membrane of Leptospira facilitates direct interactions with the environs and likely contains important constituents involved during infectio...

journal_title：Proteomics

authors： Nally JE,Whitelegge JP,Aguilera R,Pereira MM,Blanco DR,Lovett MA

更新日期：2005-01-01 00:00:00

abstract：:Caulobacter crescentus, a Gram negative alpha-purple bacterium that displays an invariant asymmetric cell division pattern, has become a key model system for the study of bacterial development. Membrane proteins play key roles in cell cycle events, both as components of landmark morphological structures and as critica...

journal_title：Proteomics

authors： Phadke ND,Molloy MP,Steinhoff SA,Ulintz PJ,Andrews PC,Maddock JR

更新日期：2001-05-01 00:00:00

abstract：:The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...

journal_title：Proteomics

authors： Yamamoto T,Langham RG,Ronco P,Knepper MA,Thongboonkerd V

更新日期：2008-06-01 00:00:00

abstract：:An aim of proteome research is to identify the entire complement of proteins expressed in defined cell types of humans, animals, plants, and microorganisms. The approach requires searching for low abundant or even rarely expressed proteins in many cell types, as well as the determination of the protein expression leve...

journal_title：Proteomics

authors： Wildgruber R,Weber G,Wise P,Grimm D,Bauer J

更新日期：2014-03-01 00:00:00

abstract：:This review provides a brief overview of the development of data-independent acquisition (DIA) mass spectrometry-based proteomics and selected DIA data analysis tools. Various DIA acquisition schemes for proteomics are summarized first including Shotgun-CID, DIA, MSE , PAcIFIC, AIF, SWATH, MSX, SONAR, WiSIM, BoxCar, S...

journal_title：Proteomics

authors： Zhang F,Ge W,Ruan G,Cai X,Guo T

更新日期：2020-09-01 00:00:00

abstract：:Protein arrays permit the parallel analysis of many different markers in a small sample volume. However, the problem of cross-reactivity limits the degree of multiplexing in parallel sandwich immunoassays (using monoclonal antibodies (mAbs)), meaning antibodies must be prescreened in order to reduce false positives. I...

journal_title：Proteomics

authors： Gilbert I,Schiffmann S,Rubenwolf S,Jensen K,Mai T,Albrecht C,Lankenau A,Beste G,Blank K,Gaub HE,Clausen-Schaumann H

更新日期：2004-05-01 00:00:00

abstract：:The function of a protein is modulated by its abundance and its degree of specific post-translational modifications such as phosphorylation, glycosylation or truncation. Consequently, changes of protein concentration and the extent of their post-translational modifications has a great influence on the activity of intr...

journal_title：Proteomics

authors： Moritz B,Meyer HE

更新日期：2003-11-01 00:00:00

abstract：:Plant tissues contain large amounts of secondary compounds that significantly interfere with protein extraction and 2DE analysis. Thus, sample preparation is a crucial step prior to 2DE in plant proteomics. This tutorial highlights the guidelines that need to be followed to perform an adequate total protein extraction...

journal_title：Proteomics

authors： Wu X,Gong F,Wang W

更新日期：2014-03-01 00:00:00