Identification and characterization of proteins interacting with SIRT1 and SIRT3: implications in the anti-aging and metabolic effects of sirtuins.

abstract：:In recent years, standardization and quality control have become important key points in industry, e.g. in drug discovery and for developing medical products. Is quality control in academic Proteomics a minor problem nowadays, where standard data formats and public repositories for data sharing exist? In this article,...

journal_title：Proteomics

authors： Eisenacher M,Schnabel A,Stephan C

更新日期：2011-03-01 00:00:00

abstract：:In recent years, proteomics research has gained importance due to increasingly powerful techniques in protein purification, mass spectrometry and identification, and due to the development of extensive protein and DNA databases from various organisms. Nevertheless, current identification methods from spectrometric dat...

journal_title：Proteomics

authors： Hernandez P,Gras R,Frey J,Appel RD

更新日期：2003-06-01 00:00:00

abstract：:Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...

journal_title：Proteomics

authors： Ahsan N,Renaut J,Komatsu S

更新日期：2009-05-01 00:00:00

abstract：:Affinity purification coupled to mass spectrometry (AP-MS) is gaining widespread use for the identification of protein-protein interactions. It is unclear, however, whether typical AP sample complexity is limiting for the identification of all protein components using standard one-dimensional LC-MS/MS. Multidimensiona...

journal_title：Proteomics

authors： Dunham WH,Larsen B,Tate S,Badillo BG,Goudreault M,Tehami Y,Kislinger T,Gingras AC

更新日期：2011-07-01 00:00:00

abstract：:The field of biochemistry is currently faced with the enormous challenge of assigning functional significance to more than thirty thousand predicted protein products encoded by the human genome. In order to accomplish this daunting task, methods will be required that facilitate the global analysis of proteins in compl...

journal_title：Proteomics

authors： Patricelli MP,Giang DK,Stamp LM,Burbaum JJ

更新日期：2001-09-01 00:00:00

abstract：:Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial-embryo cross talk and facilitate implantation remain largely unknown. Extracellular vesicles (EVs)...

journal_title：Proteomics

authors： Evans J,Rai A,Nguyen HPT,Poh QH,Elglass K,Simpson RJ,Salamonsen LA,Greening DW

更新日期：2019-12-01 00:00:00

abstract：:Helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. In addition H. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are a...

journal_title：Proteomics

authors： Hynes SO,McGuire J,Falt T,Wadström T

更新日期：2003-03-01 00:00:00

abstract：:The presence of high-abundance proteins in complex protein mixtures often masks low-abundance proteins and causes loss of resolution of 2DE. Protein fractionation steps conducted prior to 2DE can enhance the detection of low-abundance proteins and improve the resolution of 2DE. Here, we report a method to prefractiona...

journal_title：Proteomics

authors： Wang W,Wu X,Xiong E,Tai F

更新日期：2012-04-01 00:00:00

abstract：:Cells are a fundamental unit of life, and the ability to study the phenotypes and behaviors of individual cells is crucial to understanding the workings of complex biological systems. Cell phenotypes (epigenomic, transcriptomic, proteomic, and metabolomic) exhibit dramatic heterogeneity between and within the differen...

journal_title：Proteomics

authors： Mincarelli L,Lister A,Lipscombe J,Macaulay IC

更新日期：2018-09-01 00:00:00

abstract：:Transmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders characterized by the accumulation in the CNS of a pathological conformer (PrP(TSE)) of the host-encoded cellular prion protein (PrP(C)). PrP(TSE) has a central role in the pathogenesis of the disease but other factors are likely involved ...

journal_title：Proteomics

authors： Giorgi A,Di Francesco L,Principe S,Mignogna G,Sennels L,Mancone C,Alonzi T,Sbriccoli M,De Pascalis A,Rappsilber J,Cardone F,Pocchiari M,Maras B,Schininà ME

更新日期：2009-08-01 00:00:00

abstract：:Quantitative proteomic studies are contributing greatly to the understanding of the spermatozoon through the provision of detailed information on the proteins spermatozoa acquire and shed in the acquisition of fertility. Extracellular vesicles (EVs) are thought to aid in the delivery of proteins to spermatozoa in the ...

journal_title：Proteomics

authors： Leahy T,Rickard JP,Pini T,Gadella BM,de Graaf SP

更新日期：2020-06-01 00:00:00

abstract：:Accumulation of proteins in inclusions in neurological disorders is partly due to dysfunction of the ubiquitin-proteasome system. Proteasomal dysfunction may be caused by misexpression of one or more of its subunits. A large number of antibodies reactive with proteasome subunits were screened on material from patients...

journal_title：Proteomics

authors： Zouambia M,Fischer DF,Hobo B,De Vos RA,Hol EM,Varndell IM,Sheppard PW,Van Leeuwen FW

更新日期：2008-03-01 00:00:00

abstract：:The last years have seen the emergence of many large-scale proteomics initiatives that have identified thousands of new protein interactions and macromolecular assemblies. However, unfortunately, only a few among the discovered complexes meet the high-quality standards required to be promptly used in structural studie...

journal_title：Proteomics

authors： Pache RA,Aloy P

更新日期：2008-05-01 00:00:00

abstract：:Basal-like breast cancers are commonly negative for expression of estrogen and progesterone receptors and HER-2 (triple-negative breast cancer), which makes this subtype of breast cancers more aggressive and less responsive to standard treatment. We have applied a small-scale chemical proteomics method using bisindoly...

journal_title：Proteomics

authors： Dolai S,Xu Q,Liu F,Molloy MP

更新日期：2011-07-01 00:00:00

abstract：:Articles assembled in the second part of this Special Issue describe some experimental and computational approaches for the structural and functional characterization of intrinsically disordered proteins. Since these tools represent specialized gear for the focused analysis of various aspects of dark proteome, they ca...

journal_title：Proteomics

authors： Uversky VN

更新日期：2019-03-01 00:00:00

abstract：:Proteolysis affects every protein at some point in its life cycle. Many biomarkers of disease or cancer are stable proteolytic fragments in biological fluids. There is great interest and a challenge in proteolytically modified protein study to identify physiologic protease-substrate relationships and find potential bi...

journal_title：Proteomics

authors： Shen C,Yu Y,Li H,Yan G,Liu M,Shen H,Yang P

更新日期：2012-06-01 00:00:00

abstract：:The Japanese red and pink corals are known to be precious because of their commercial value resulting from their use in ornaments, jewelry, and medicine. Precious corals are very interesting models for biomineralization studies and possess two different skeletal structures: an axial skeleton and an endoskeleton (scler...

journal_title：Proteomics

authors： Rahman MA,Karl K,Nonaka M,Fujimura H,Shinjo R,Oomori T,Wörheide G

更新日期：2014-11-01 00:00:00

abstract：:The HUPO Brain Proteome Project (HUPO BPP) held its 12th workshop in Toronto on 26 September 2009 prior to the HUPO VIII World Congress. The principal aim of this project is to obtain a better understanding of neurodiseases and ageing, with the ultimate objective of discovering prognostic and diagnostic biomarkers, in...

journal_title：Proteomics

authors： Gröttrup B,Eisenacher M,Stephan C,Marcus K,Lee B,Meyer HE,Park YM

更新日期：2010-06-01 00:00:00

abstract：:A diverse range of proteins are modified by the post-translational covalent attachment of a glycosyl-phosphatidylinositol (GPI) membrane anchor. Hydropathy plots and other computer algorithms can be used to predict the presence of a GPI anchor attachment signal in the nascent polypeptide chain. However, the presence o...

journal_title：Proteomics

authors： Hooper NM

更新日期：2001-06-01 00:00:00

abstract：:Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age-related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its...

journal_title：Proteomics

authors： Kamita M,Mori T,Sakai Y,Ito S,Gomi M,Miyamoto Y,Harada A,Niida S,Yamada T,Watanabe K,Ono M

更新日期：2015-05-01 00:00:00

abstract：:Protein expression changes induced in thioglycolate-elicited peritoneal murine macrophages (M Phi) by infection with type III Group B Streptococcus (GBS) are described. Proteins from control M Phi and M Phi incubated 2 h with live or heat-inactivated GBS were separated by 2-DE. Proteins whose expression was significan...

journal_title：Proteomics

authors： Susta F,Chiasserini D,Fettucciari K,Orvietani PL,Quotadamo F,Noce R,Bartoli A,Marconi P,Corazzi L,Binaglia L

更新日期：2010-06-01 00:00:00

abstract：:Abnormal phosphorylation contributes to the formation of neurofibrillary tangles in Alzheimer's disease (AD), but may play other signaling roles during AD pathogenesis. In this study, we employed IMAC followed by LC-MS/MS to identify phosphopeptides from eight individual AD and eight age-matched control postmortem hum...

journal_title：Proteomics

authors： Dammer EB,Lee AK,Duong DM,Gearing M,Lah JJ,Levey AI,Seyfried NT

更新日期：2015-01-01 00:00:00

abstract：:Isolated from Elephantopus scaber L., a Chinese medicinal herb that is widely used to prevent and treat cancers in China, isodeoxyelephantopin (ESI) exerted antitumor effects on several cancer cells. However, its antitumor mechanism is still not clear. In this study, we found that ESI could induce G2/M arrest and subs...

journal_title：Proteomics

authors： Yan GR,Tan Z,Wang Y,Xu ML,Yu G,Li Y,He QY

更新日期：2013-11-01 00:00:00

abstract：:MS-based protein identification is an important part of both gel-based and gel-free proteome studies. The MASCOT search engine (http://www.matrixscience.com) provides one of the most popular automated algorithms for this task. Here we present an open-source software library written in Java that parses raw MASCOT resul...

journal_title：Proteomics

authors： Helsens K,Martens L,Vandekerckhove J,Gevaert K

更新日期：2007-02-01 00:00:00

abstract：:Warm ischemia reperfusion (IR) injury of the liver is associated with changes in the expression and/or post-translational modification of numerous proteins. Only a few of these have been identified. We used 2-D DIGE to identify cytosolic proteins altered in the early stage of IR in an established rat model of segmenta...

journal_title：Proteomics

authors： Wilson CH,Zeile S,Chataway T,Nieuwenhuijs VB,Padbury RT,Barritt GJ

更新日期：2011-11-01 00:00:00

abstract：:In proteomic research, two-dimensional electrophoresis (2-D) is an important tool for investigating differential patterns of qualitative and quantitative protein expression. The strength of the technique is due to its unrivalled power of being able to separate simultaneously thousands of proteins. The key to the compa...

journal_title：Proteomics

authors： Veeser S,Dunn MJ,Yang GZ

更新日期：2001-07-01 00:00:00

abstract：:Most information on molecular processes accompanying and driving adipocyte differentiation are derived from rodent models. Here, we provide a comprehensive analysis of combined transcriptomic and proteomic alterations during adipocyte differentiation in Simpson-Golabi-Behmel Syndrome (SGBS) cells. The SGBS cells are a...

journal_title：Proteomics

authors： Kalkhof S,Krieg L,Büttner P,Wabitsch M,Küntzel C,Friebe D,Landgraf K,Hanschkow M,Schubert K,Kiess W,Krohn K,Blüher M,von Bergen M,Körner A

更新日期：2020-05-08 00:00:00

abstract：:Sustainable innovations in sequencing technologies have resulted in a torrent of microbial genome sequencing projects. However, the prokaryotic genomes sequenced so far are unequally distributed along their phylogenetic tree; few phyla contain the majority, the rest only a few representatives. Accurate genome annotati...

journal_title：Proteomics

authors： Kumar D,Mondal AK,Kutum R,Dash D

更新日期：2016-01-01 00:00:00

abstract：:Most MS-based glycomic and glycoproteomic analyses focus on identifying changes in terminal glyco-epitopes represented by sialylation and fucosylation at specific positions of the terminal N-acetyllactosamine units. Much less attention was accorded to the underlying linear or branched poly-N-acetyllactosamine extensio...

journal_title：Proteomics

authors： Wang SH,Wu SW,Khoo KH

更新日期：2011-07-01 00:00:00

abstract：:Recent advances in two-dimensional electrophoresis (2-DE) such as fluorescent 2-D differential gel electrophoresis (2-D DIGE) has made it possible to detect and quantitate the critical changes involved in disease pathogenesis. We have previously identified novel proteins with altered expression in primary colorectal c...

journal_title：Proteomics

authors： Nishimori T,Tomonaga T,Matsushita K,Oh-Ishi M,Kodera Y,Maeda T,Nomura F,Matsubara H,Shimada H,Ochiai T

更新日期：2006-02-01 00:00:00