Abstract:
:The last years have seen the emergence of many large-scale proteomics initiatives that have identified thousands of new protein interactions and macromolecular assemblies. However, unfortunately, only a few among the discovered complexes meet the high-quality standards required to be promptly used in structural studies. This has thus created an increasing gap between the number of known protein interactions and complexes and those for which a high-resolution 3-D structure is available. Here, we present and validate a computational strategy to distinguish those complexes found in high-throughput affinity purification experiments that will stand the best chances to successfully express, purify and crystallize with little further intervention. Our method suggests that there are some 50 complexes recently discovered in yeast that could readily enter the structural biology pipelines.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Pache RA,Aloy Pdoi
10.1002/pmic.200700966subject
Has Abstractpub_date
2008-05-01 00:00:00pages
1959-64issue
10eissn
1615-9853issn
1615-9861journal_volume
8pub_type
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