Abstract:
:The proteins recovered in exhaled breath condensate (EBC) might be used to non-invasively monitor respiratory diseases. However, the range of proteins and their source are still unresolved and contamination by saliva or a similar protein pattern in the nasal and bronchial compartments may make interpretation of the data difficult. We studied nasal EBC (collected through a "free of touch" technique by negative pressure), oral tidal, and oral forced EBC (collected through a rebreathing valve as a saliva trap connected to tubing submerged into ice) and matched saliva samples from five healthy adult subjects. The protein samples were separated by two-dimensional electrophoresis and the silver stained gels were analyzed by Melanie 2 software. In both nasal and oral EBC, three spots (72 kDa/isoelectric point (pI) 6.6-7.0, 66 kDa/pI 5.9-6.7 and 45-48 kDa/pI 8.0-8.6) were consistently present in all subjects. Several other proteins were only sporadically detected. Despite improbable saliva contamination (no phosphorus contamination in the same oral and nasal EBC, no amylase activity in 10 pairs of nasal and oral EBC collected by the same technique), on average 63% and 71% of the spots identified in oral and nasal EBC were also found in the matched individual saliva samples. Compared to saliva, the range and amount of protein in all types of EBC was very small. Even when collected free of saliva contamination the majority of proteins present in EBC was also found in saliva, suggesting that these proteins are present in both compartments, e.g. saliva and secretions of the lower airspaces. The quantification and identification of specific proteins in the various compartments is warranted in future studies to determine the practical value of EBC.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Griese M,Noss J,von Bredow Cdoi
10.1002/1615-9861(200206)2:6<690::AID-PROT690>3.0.subject
Has Abstractpub_date
2002-06-01 00:00:00pages
690-6issue
6eissn
1615-9853issn
1615-9861journal_volume
2pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200600373
更新日期:2006-12-01 00:00:00
abstract::To date, proteomics approaches have aimed to either identify novel proteins or change in protein expression/modification in various organisms under normal or disease conditions. One major aspect of functional proteomics is to identify protein biological properties in a given context, however, forward proteomics approa...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200600211
更新日期:2006-10-01 00:00:00
abstract::Previous investigations of bovine seminal plasma (BSP) have revealed the identities of the three major proteins, BSP-PDC109, BSP-A3 and BSP-30 kDa, which together constitute about half of the total protein, as well as about 30 of the minor proteins. Analyses of BSP by 2-DE have revealed about 250 protein spots, sugges...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500830
更新日期:2006-11-01 00:00:00
abstract::Brucella melitensis is a facultative intracellular bacterial pathogen that causes brucellosis, a zoonotic disease primarily infecting sheep and goats, characterized by undulant fever, arthritic pain and other neurological disorders in humans. A comprehensive proteomic study of strain 16M was conducted to identify and ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200208)2:8<1047::AID-PROT1047>3.
更新日期:2002-08-01 00:00:00
abstract::The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse live...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000126
更新日期:2010-11-01 00:00:00
abstract::Intrinsically disordered proteins (IDPs) are implicated in a range of human diseases, some of which are associated with the ability to bind to lipids. Although the presence of solvent-exposed hydrophobic regions in IDPs should favor their interactions with low-molecular-weight hydrophobic/amphiphilic compounds, this h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800098
更新日期:2019-03-01 00:00:00
abstract::The use of proteomics for efficient, accurate, and complete analysis of clinical samples poses a variety of technical challenges. The presence of higher abundance proteins in the plasma, such as albumin, may mask the detection of lower abundance proteins such as the cytokines. Methods have been proposed to deplete the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401331
更新日期:2005-12-01 00:00:00
abstract::New insights on cellular heterogeneity in the last decade provoke the development of a variety of single cell omics tools at a lightning pace. The resultant high-dimensional single cell data generated by these tools require new theoretical approaches and analytical algorithms for effective visualization and interpreta...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201600267
更新日期:2017-02-01 00:00:00
abstract::Mechanisms of milk lipid secretion are highly controversial. Analyzing the fine protein composition of the "milk fat globule membrane" (MFGM), the triple-layered membrane surrounding milk lipid droplets (LDs) can provide mechanistic clues to better understand LD biosynthesis and secretion pathways in mammary epithelia...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400245
更新日期:2015-07-01 00:00:00
abstract::Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. Ho...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800209
更新日期:2019-01-01 00:00:00
abstract::Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60 min at 8...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000597
更新日期:2011-04-01 00:00:00
abstract::Each postnatal hair follicle (HF) perpetually goes through three phases: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still largely unknown. Our previous study shows that the keratinocyte specific Smad4 knockout mice exhibit progressive alopecia due ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700760
更新日期:2008-06-01 00:00:00
abstract::We have combined high-resolution two-dimensional (2-D) gel electrophoresis and mass spectrometry with the aim of identifying proteins represented in the 2-D gel database of the wing imaginal discs of Drosophila melanogaster. First, we obtained a high-resolution 2-D gel pattern of [35S]methionine + [35S]cysteine-labele...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400923
更新日期:2005-02-01 00:00:00
abstract::Unconjugated bilirubin (UCB) is a powerful antioxidant and a modulator of cell growth through the interaction with several signal transduction pathways. Although newborns develop a physiological jaundice, in case of severe hyperbilirubinemia UCB may become neurotoxic causing severe long-term neuronal damages, also kno...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900579
更新日期:2010-04-01 00:00:00
abstract::Aphids are major insect pests of cereal crops, acting as virus vectors as well as causing direct damage. The responses of wheat to infestation by cereal aphid (Sitobion avenae) were investigated in a proteomic analysis. Approximately, 500 protein spots were reproducibly detected in the extracts from leaves of wheat se...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900801
更新日期:2011-05-01 00:00:00
abstract::The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis proce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100313
更新日期:2012-01-01 00:00:00
abstract::High-density lipoprotein (HDL) is the most abundant lipoprotein particle in the plasma and a negative risk factor of atherosclerosis. By using a proteomic approach it is possible to obtain detailed information about its protein content and protein modifications that may give new information about the physiological rol...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401010
更新日期:2005-04-01 00:00:00
abstract::Citrulline (Cit) actions on muscle metabolism remain unclear. Those latter were investigated using a proteomic approach on Tibialis muscles from male Sprague-Dawley rats. At 23 months of age, rats were either fed ad libitum (AL group) or subjected to dietary restriction for 12 weeks. At the end of the restriction peri...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200262
更新日期:2013-07-01 00:00:00
abstract::Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% of CW mass) and proteins (5-10%) that play major roles in CW plasticity during development and in response to environmental cues. Here, we present CW proteomics data of Arabidopsis thaliana roots. Plants were cultivated in hydroponic conditio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500129
更新日期:2016-02-01 00:00:00
abstract::Sarcopenia, defined as age-associated decline of muscle mass and function, is a risk factor for mortality and disability, and comorbid with several chronic diseases such as type II diabetes and cardiovascular diseases. Clinical trials showed that nutritional supplements had positive effects on muscle mass, but not on ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800411
更新日期:2020-03-01 00:00:00
abstract::The use of anthrax spores as a bioweapon has spurred efforts aimed at identifying key proteins expressed in Bacillus anthracis. Because spore germination and outgrowth occur prior to and are required for disease manifestations, blocking germination and early outgrowth with novel vaccines or inhibitors targeting critic...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400831
更新日期:2004-09-01 00:00:00
abstract::Sporozoites of the malaria parasite Plasmodium are transmitted by mosquitoes and infect the liver for an initial and obligatory round of replication, before exponential multiplication in the blood and onset of the disease. Sporozoites and liver stages provide attractive targets for malaria vaccines and prophylactic dr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000305
更新日期:2021-01-16 00:00:00
abstract::"Liquid biopsies" have received attention as a complementary tool for traditional tissue biopsies that may enhance the spectrum of analysis for tumor-derived factors. One such factor gaining prominence in the liquid biopsy field is extracellular vesicles (EVs), membrane-bound nanovesicles which are secreted by cells i...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800155
更新日期:2019-01-01 00:00:00
abstract::Plant tissues contain large amounts of secondary compounds that significantly interfere with protein extraction and 2DE analysis. Thus, sample preparation is a crucial step prior to 2DE in plant proteomics. This tutorial highlights the guidelines that need to be followed to perform an adequate total protein extraction...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300239
更新日期:2014-03-01 00:00:00
abstract::The hepatitis B virus X-protein (HBx), a multifunctional viral regulator, participates in the viral life cycle and in the development of hepatocellular carcinoma (HCC). We previously reported a high incidence of HCC in transgenic mice expressing HBx. In this study, proteomic analysis was performed to identify proteins...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800779
更新日期:2009-11-01 00:00:00
abstract::Changing smooth muscle phenotype and abnormal cell proliferation are important features of vascular pathology, including the failure of saphenous vein bypass grafts. We have characterised and mapped protein expression in human saphenous vein medial smooth muscle, using two-dimensional (2-D) polyacrylamide gel electrop...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/1615-9861(200111)1:11<1405::AID-PROT1405>3
更新日期:2001-11-01 00:00:00
abstract::The gut microbiome has been shown to play a significant role in human healthy and diseased states. The dynamic signaling that occurs between the host and microbiome is critical for the maintenance of host homeostasis. Analyzing the human microbiome with metaproteomics, metabolomics, and integrative multi-omics analyse...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201800363
更新日期:2019-08-01 00:00:00
abstract::The pilot phase of the Human Brain Proteome Project as a part of the Human Proteome Organisation has just been started. In two pilot studies, 18 different laboratories are analyzing mouse brains of three age stages and human brain autopsy versus biopsy material, respectively. The overall aim is to elucidate the portfo...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400867
更新日期:2004-08-01 00:00:00
abstract::High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effec...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200402086
更新日期:2006-03-01 00:00:00
abstract::Aberrant telomerase reactivation in differentiated cells represents a major event in oncogenic transformation. Recurrent somatic mutations in the human telomerase reverse transcriptase (TERT) promoter region, predominantly localized to two nucleotide positions, are highly prevalent in many cancer types. Both mutations...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500327
更新日期:2016-02-01 00:00:00