Growth condition-dependent cell surface proteome analysis of Enterococcus faecium.

abstract：:With an emphasis on obtaining a multitude of high quality tandem mass spectrometry spectra for protein identification, instrumental parameters are described for the liquid chromatography-tandem mass spectrometry analysis of trypsin digested unfractionated urine using a hybrid quadrupole-time-of-flight (Q-TOF) mass spe...

journal_title：Proteomics

authors： Davis MT,Spahr CS,McGinley MD,Robinson JH,Bures EJ,Beierle J,Mort J,Yu W,Luethy R,Patterson SD

更新日期：2001-01-01 00:00:00

abstract：:Several lines of evidence suggest that hnRNPs A/B (hnRNPs A1, A2/B1, and A3) play an important role in proliferation, although the functional overlap among members of hnRNPs A/B remains largely unknown. In this study, we have employed RNAi knockdown and proteomic approaches to investigate the biological functions of h...

journal_title：Proteomics

authors： Yeh YM,Chen CY,Huang PR,Hsu CW,Wu CC,Wang TC

更新日期：2014-06-01 00:00:00

abstract：:Recent advances in two-dimensional electrophoresis (2-DE) such as fluorescent 2-D differential gel electrophoresis (2-D DIGE) has made it possible to detect and quantitate the critical changes involved in disease pathogenesis. We have previously identified novel proteins with altered expression in primary colorectal c...

journal_title：Proteomics

authors： Nishimori T,Tomonaga T,Matsushita K,Oh-Ishi M,Kodera Y,Maeda T,Nomura F,Matsubara H,Shimada H,Ochiai T

更新日期：2006-02-01 00:00:00

abstract：:Selected or multiple reaction monitoring is a targeted mass spectrometry method (S/MRM-MS), in which many peptides are simultaneously and consistently analyzed during a single liquid chromatography-mass spectrometry (LC-S/MRM-MS) measurement. These capabilities make S/MRM-MS an attractive method to monitor a consisten...

journal_title：Proteomics

authors： Ebhardt HA,Sabidó E,Hüttenhain R,Collins B,Aebersold R

更新日期：2012-04-01 00:00:00

abstract：:Human galectin-3 (hGal-3) is a mammalian lectin involved in regulation of RNA splicing, apoptosis, cell differentiation, and proliferation. Multimerized extracellular hGal-3 is thought to crosslink cells by binding to glycoproteins and glycosylated cancer antigens on the cell surface or extracellular matrix. Fluoresce...

journal_title：Proteomics

authors： Bogoeva VP,Varriale A,John CM,D'Auria S

更新日期：2010-05-01 00:00:00

abstract：:The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this st...

journal_title：Proteomics

authors： Lubelwana Hafver T,Wanichawan P,Manfra O,de Souza GA,Lunde M,Martinsen M,Louch WE,Sejersted OM,Carlson CR

更新日期：2017-09-01 00:00:00

abstract：:Human butyrophilin (BTN) expression in milk fat globule (MFGM) was evaluated using two dimensional electrophoresis (2-DE) as the separation technique, and peptide mass mapping by matrix-assisted laser desorption/ionisation-mass spectrometry (MALDI-MS) as the identification tool. Since milk composition changes througho...

journal_title：Proteomics

authors： Cavaletto M,Giuffrida MG,Fortunato D,Gardano L,Dellavalle G,Napolitano L,Giunta C,Bertino E,Fabris C,Conti A

更新日期：2002-07-01 00:00:00

abstract：:Staphylococcus aureus, a bacterial, food-borne pathogen of humans, can contaminate raw fruits and vegetables. While physical and chemical methods are available to control S. aureus, scientists are searching for inhibitory phytochemicals from plants. One promising compound from pomegranate is punicalagin, a natural ant...

journal_title：Proteomics

authors： Cooper B,Islam N,Xu Y,Beard HS,Garrett WM,Gu G,Nou X

更新日期：2018-05-01 00:00:00

abstract：:Glycosylphosphatidylinositol-anchored proteins (GPI-APs) are an important class of glycoproteins that are tethered to the surface of mammalian cells via the lipid GPI. GPI-APs have been implicated in many important cellular functions including cell adhesion, cell signaling, and immune regulation. Proteomic identificat...

journal_title：Proteomics

authors： Cortes LK,Vainauskas S,Dai N,McClung CM,Shah M,Benner JS,Corrêa IR Jr,VerBerkmoes NC,Taron CH

更新日期：2014-11-01 00:00:00

abstract：:Campylobacter jejuni is a major gastrointestinal pathogen that colonizes host mucosa via interactions with extracellular matrix proteins, such as fibronectin (Fn). Fn-binding is mediated by a 37 kDa outer membrane protein termed Campylobacter adherence Factor (CadF). The outer membrane protein profile of a recent gast...

journal_title：Proteomics

authors： Scott NE,Marzook NB,Deutscher A,Falconer L,Crossett B,Djordjevic SP,Cordwell SJ

更新日期：2010-01-01 00:00:00

abstract：:Protein interactions have been at the focus of computational biology in recent years. In particular, interest has come from two different communities--structural and systems biology. Here, we will discuss key systems and structural biology methods that have been used for analysis and prediction of protein-protein inte...

journal_title：Proteomics

authors： Hooda Y,Kim PM

更新日期：2012-05-01 00:00:00

abstract：:In recent years, a new paradigm for genome annotation has emerged, termed "proteogenomics," that leverages peptide MS to annotate a genome. This is achieved by mapping peptides to a six-frame translation of a genome, including available splice databases, which may suggest refinements to gene models. Using this approac...

journal_title：Proteomics

authors： Chapman B,Bellgard M

更新日期：2014-12-01 00:00:00

abstract：:We here present the jmzReader library: a collection of Java application programming interfaces (APIs) to parse the most commonly used peak list and XML-based mass spectrometry (MS) data formats: DTA, MS2, MGF, PKL, mzXML, mzData, and mzML (based on the already existing API jmzML). The library is optimized to be used i...

journal_title：Proteomics

authors： Griss J,Reisinger F,Hermjakob H,Vizcaíno JA

更新日期：2012-03-01 00:00:00

abstract：:Aedes aegypti females ingest sugar or blood to obtain the nutrients needed to maintain cellular homeostasis. During human blood ingestion, female mosquitoes may transmit different viruses such as dengue, yellow fever and, more recently, zika and chikungunya. Here, we report changes in protein expression in the heads o...

journal_title：Proteomics

authors： Nunes AT,Brito NF,Oliveira DS,Araujo GD,Nogueira FC,Domont GB,Moreira MF,Moreira LM,Soares MR,Melo AC

更新日期：2016-10-01 00:00:00

abstract：:The International Plant Proteomics Organization (INPPO) is a global platform of the plant proteomics community or, more generally, the scientific community that uses proteomics to address plant biology. Organizing an international conference is one of its initiatives to promote plant proteomics by involving and gather...

journal_title：Proteomics

authors： Lüthje S,Renaut J,Job D,Hajduch M,Carpentier S,Sarkar A,Agrawal R,Dunn MJ,Rakwal R,Agrawal GK

更新日期：2015-05-01 00:00:00

abstract：:Proteins and peptides serve as biomarkers in the context of multiple pathologies. The hypothesis that protein or peptide biomarkers may also be of value in the context of the Covid-19 pandemic appears self-evident. Proteome based biomarkers are not expected to display significant added value in the detection of viral ...

journal_title：Proteomics

authors： Jankowski J

更新日期：2020-12-01 00:00:00

abstract：:Exercise training has been recommended as a nonpharmacological strategy for the prevention and attenuation of skeletal muscle atrophy in distinct pathophysiological conditions. Despite the well-established phenotypic alterations, the molecular mechanisms underlying exercise-induced skeletal muscle remodeling are poorl...

journal_title：Proteomics

authors： Padrão AI,Ferreira R,Amado F,Vitorino R,Duarte JA

更新日期：2016-03-01 00:00:00

abstract：:Mammalian cells secrete two types of extracellular vesicles either constitutively or in a regulated manner: exosomes (50-100 nm in diameter) released from the intracellular compartment and ectosomes (also called microvesicles, 100-1000 nm in diameter) shed directly from the plasma membrane. Extracellular vesicles are ...

journal_title：Proteomics

authors： Choi DS,Kim DK,Kim YK,Gho YS

更新日期：2013-05-01 00:00:00

abstract：:Absolute protein concentration determination is becoming increasingly important in a number of fields including diagnostics, biomarker discovery and systems biology modeling. The recently introduced quantification concatamer methodology provides a novel approach to performing such determinations, and it has been appli...

journal_title：Proteomics

authors： Swainston N,Jameson D,Carroll K

更新日期：2011-01-01 00:00:00

abstract：:A proteomic analysis of a wild-type and of a phoB mutant showed that Vibrio cholerae expresses genes of two major regulons in response to phosphate starvation. The Pho regulon, expressed by the wild-type, allowed the cells to adapt to the new environment. Induction of the general stress regulon was mainly observed in ...

journal_title：Proteomics

authors： von Krüger WM,Lery LM,Soares MR,de Neves-Manta FS,Batista e Silva CM,Neves-Ferreira AG,Perales J,Bisch PM

更新日期：2006-03-01 00:00:00

abstract：:Until now, no data are available on the outer membrane (OM) proteome of Erwinia amylovora, a Gram-negative plant pathogen, causing fire blight in most of the members of the Rosaceae family. Since the OM forms the interface between the bacterial cell and its environment it is in direct contact with the host. Additional...

journal_title：Proteomics

authors： Holtappels M,Noben JP,Valcke R

更新日期：2016-09-01 00:00:00

abstract：:Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins ...

journal_title：Proteomics

authors： Janjanam J,Jamwal M,Singh S,Kumar S,Panigrahi AK,Hariprasad G,Jena MK,Anand V,Kumar S,Kaushik JK,Dang AK,Mukesh M,Mishra BP,Srinivasan A,Reddy VS,Mohanty AK

更新日期：2013-11-01 00:00:00

abstract：:Proteomics is increasingly being used to understand enzyme expression and regulatory mechanisms involved in the accumulation of storage reserves in crops with sequenced genomes. During the past six years, considerable progress has been made to characterize proteomes of both mature and developing seeds, particularly oi...

journal_title：Proteomics

authors： Hajduch M,Matusova R,Houston NL,Thelen JJ

更新日期：2011-05-01 00:00:00

abstract：:Legume symbiosis with rhizobia results in the formation of a specialized organ, the root nodule, where atmospheric dinitrogen is reduced to ammonia. In Lotus japonicus (Lotus), several genes involved in nodule development or nodule function have been defined using biochemistry, genetic approaches, and high-throughput ...

journal_title：Proteomics

authors： Dam S,Dyrlund TF,Ussatjuk A,Jochimsen B,Nielsen K,Goffard N,Ventosa M,Lorentzen A,Gupta V,Andersen SU,Enghild JJ,Ronson CW,Roepstorff P,Stougaard J

更新日期：2014-02-01 00:00:00

abstract：:The Ser/Thr/Tyr phosphoproteome of Bacillus subtilis was analyzed by a 2-D gel-based approach combining Pro-Q Diamond staining and [(33)P]-labeling. In exponentially growing B. subtilis cells 27 proteins could be identified after staining with Pro-Q Diamond and/or [(33)P]-labeling and one additional protein was labele...

journal_title：Proteomics

authors： Eymann C,Becher D,Bernhardt J,Gronau K,Klutzny A,Hecker M

更新日期：2007-10-01 00:00:00

abstract：:In spite of the high sensitivity of silver staining and the wide dynamic range of various fluorescent detection methods, Coomassie Brilliant Blue staining is still the most widely used protein detection technique for proteins separated by polyacrylamide gel electrophoresis. There are several reasons: Low price, Visibl...

journal_title：Proteomics

authors： Westermeier R

更新日期：2006-09-01 00:00:00

abstract：:The eukaryotic chromatin/chromosome stores genomic information, controls genetic material distribution, and plays an essential role in the establishment and maintenance of spatial and temporal gene expression profile. Despite over a century of research, the protein composition and higher level structure of chromatin s...

journal_title：Proteomics

authors： Tan F,Li G,Chitteti BR,Peng Z

更新日期：2007-12-01 00:00:00

abstract：:As mass-spectrometry-based proteomics has matured during the past decade, a growing emphasis has been placed on quality control. For this purpose, multiple computational quality control tools have been introduced. These tools generate a set of metrics that can be used to assess the quality of a mass spectrometry exper...

journal_title：Proteomics

authors： Bittremieux W,Valkenborg D,Martens L,Laukens K

更新日期：2017-02-01 00:00:00

abstract：:This study describes the gel-free phosphoproteomic analysis of the phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea grown in vitro under nonlimiting conditions. Using a combination of strong cation exchange and IMAC prior to LC-MS, we identified over 1350 phosphopeptides per fungus representing over ...

journal_title：Proteomics

authors： Davanture M,Dumur J,Bataillé-Simoneau N,Campion C,Valot B,Zivy M,Simoneau P,Fillinger S

更新日期：2014-07-01 00:00:00

abstract：:The CpG motif of bacterial DNA (CpG-DNA) is a potent immunostimulating agent whose mechanism of action is not yet clear. Here, we used both DNA microarray and proteomic approaches to investigate the effects of oligodeoxynucleotides containing the CpG motif (CpG-ODN) on gene transcription and protein expression profile...

journal_title：Proteomics

authors： Kuo CC,Kuo CW,Liang CM,Liang SM

更新日期：2005-03-01 00:00:00