Growth condition-dependent cell surface proteome analysis of Enterococcus faecium.


:The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was grown until mid-log phase in brain heart infusion medium (BHI) with, or without 0.02% bile salts, Tryptic Soy Broth with 1% glucose (TSBg) and urine, and its cell surface was "shaved" using immobilized trypsin. Peptides were identified using MS/MS. Mapping against the translated E1162 whole genome sequence identified 67 proteins that were differentially detected in different conditions. In urine, 14 proteins were significantly more and nine proteins less abundant relative to the other conditions. Growth in BHI-bile and TSBg, revealed four and six proteins, respectively, which were uniquely present in these conditions while two proteins were uniquely present in both conditions. Thus, proteolytic shaving of E. faecium cells identified differentially surface exposed proteins in different growth conditions. These proteins are of special interest as they provide more insight in the adaptive mechanisms and may serve as targets for the development of novel therapeutics against this multi-resistant emerging pathogen. All MS data have been deposited in the ProteomeXchange with identifier PXD002497 (






Sinnige JC,de Been M,Zhou M,Bonten MJ,Willems RJ,Top J




Has Abstract


2015-11-01 00:00:00












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    authors: Heller M,Watts JD,Aebersold R

    更新日期:2001-01-01 00:00:00

  • Efficient prefractionation of low-abundance proteins in human plasma and construction of a two-dimensional map.

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    更新日期:2005-08-01 00:00:00

  • Contribution of the antibodies response induced by a low virulent Candida albicans strain in protection against systemic candidiasis.

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    authors: Fernández-Arenas E,Molero G,Nombela C,Diez-Orejas R,Gil C

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    authors: Guergues J,Zhang P,Liu B,Stevens SM Jr

    更新日期:2019-06-01 00:00:00

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    authors: Xu Z,Zhou X,Lu H,Wu N,Zhao H,Zhang L,Zhang W,Liang YL,Wang L,Liu Y,Yang P,Zha X

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  • 2-D DIGE analysis of the mitochondrial proteome from human skeletal muscle reveals time course-dependent remodelling in response to 14 consecutive days of endurance exercise training.

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    authors: Egan B,Dowling P,O'Connor PL,Henry M,Meleady P,Zierath JR,O'Gorman DJ

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    authors: Beasley CL,Pennington K,Behan A,Wait R,Dunn MJ,Cotter D

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  • Proteomics analyses of prostate cancer cells reveal cellular pathways associated with androgen resistance.

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    authors: Höti N,Shah P,Hu Y,Yang S,Zhang H

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    authors: Sarto C,Déon C,Doro G,Hochstrasser DF,Mocarelli P,Sanchez JC

    更新日期:2001-10-01 00:00:00

  • Drug penetration and metabolism in 3D cell cultures treated in a 3D printed fluidic device: assessment of irinotecan via MALDI imaging mass spectrometry.

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    pub_type: 杂志文章


    authors: LaBonia GJ,Lockwood SY,Heller AA,Spence DM,Hummon AB

    更新日期:2016-06-01 00:00:00

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    authors: Niggeweg R,Köcher T,Gentzel M,Buscaino A,Taipale M,Akhtar A,Wilm M

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    authors: Casado B,Pannell LK,Iadarola P,Baraniuk JN

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  • Proteomic analysis of interactors for yeast protein arginine methyltransferase Hmt1 reveals novel substrate and insights into additional biological roles.

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    authors: Jackson CA,Yadav N,Min S,Li J,Milliman EJ,Qu J,Chen YC,Yu MC

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    authors: Tolin S,Arrigoni G,Trentin AR,Veljovic-Jovanovic S,Pivato M,Zechman B,Masi A

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    authors: Eisenacher M,Schnabel A,Stephan C

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    authors: Deighton RF,Kerr LE,Short DM,Allerhand M,Whittle IR,McCulloch J

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    authors: Wang W,Wu X,Xiong E,Tai F

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    authors: Helsens K,Martens L,Vandekerckhove J,Gevaert K

    更新日期:2007-02-01 00:00:00

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    authors: Iqbal J,Li W,Ullah K,Hasan M,Linna G,Awan U,Zhang Y,Batool S,Qing H,Deng Y

    更新日期:2013-08-01 00:00:00

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    pub_type: 杂志文章


    authors: Wu X,Gong F,Wang W

    更新日期:2014-03-01 00:00:00

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    authors: Li HD,Menon R,Omenn GS,Guan Y

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    authors: Ferry N,Stavroulakis S,Guan W,Davison GM,Bell HA,Weaver RJ,Down RE,Gatehouse JA,Gatehouse AM

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  • Maize (Zea mays L.) seedling leaf nuclear proteome and differentially expressed proteins between a hybrid and its parental lines.

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    pub_type: 杂志文章


    authors: Guo B,Chen Y,Li C,Wang T,Wang R,Wang B,Hu S,Du X,Xing H,Song X,Yao Y,Sun Q,Ni Z

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  • Urinary Proteomics Associates with COVID-19 Severity: Pilot Proof-of-Principle Data and Design of a Multicentric Diagnostic Study.

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    authors: Wendt R,Kalbitz S,Lübbert C,Kellner N,Macholz M,Schroth S,Ermisch J,Latosisnka A,Arnold B,Mischak H,Beige J,Metzger J

    更新日期:2020-09-10 00:00:00

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    pub_type: 杂志文章,评审


    authors: Ray S,Reddy PJ,Jain R,Gollapalli K,Moiyadi A,Srivastava S

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    pub_type: 杂志文章


    authors: Parada C,Gato A,Aparicio M,Bueno D

    更新日期:2006-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Victor B,Kanobana K,Gabriël S,Polman K,Deckers N,Dorny P,Deelder AM,Palmblad M

    更新日期:2012-06-01 00:00:00

  • Microdissection-An Essential Prerequisite for Spatial Cancer Omics.

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    pub_type: 杂志文章,评审


    authors: von Eggeling F,Hoffmann F

    更新日期:2020-09-01 00:00:00

  • Two-dimensional liquid chromatography/tandem mass spectrometry analysis of Gradiflow fractionated native human plasma.

    abstract::One of the major challenges facing protein analysis is the dynamic range of protein expression within massively complex samples (Corthals, G. L. et al.., Electrophoresis 2000, 21, 1104-1115). In plasma this difference is as great as ten orders of magnitude, and this is currently beyond the range of detection achievabl...


    pub_type: 杂志文章


    authors: Wasinger VC,Locke VL,Raftery MJ,Larance M,Rothemund D,Liew A,Bate I,Guilhaus M

    更新日期:2005-08-01 00:00:00

  • Optimization of evanescent-field fluorescence-assisted lectin microarray for high-sensitivity detection of monovalent oligosaccharides and glycoproteins.

    abstract::Lectin microarray is an emerging technique, which will accelerate glycan profiling and discovery of glycan-related biomarkers. One of the most important stages in realizing the potential of the technique is to achieve sufficiently high sensitivity to detect even the low concentrations of some target glycoproteins whic...


    pub_type: 杂志文章


    authors: Uchiyama N,Kuno A,Tateno H,Kubo Y,Mizuno M,Noguchi M,Hirabayashi J

    更新日期:2008-08-01 00:00:00