Proteomic analysis in human breast cancer: identification of a characteristic protein expression profile of malignant breast epithelium.

abstract：:Citrulline (Cit) actions on muscle metabolism remain unclear. Those latter were investigated using a proteomic approach on Tibialis muscles from male Sprague-Dawley rats. At 23 months of age, rats were either fed ad libitum (AL group) or subjected to dietary restriction for 12 weeks. At the end of the restriction peri...

journal_title：Proteomics

authors： Faure C,Morio B,Chafey P,Le Plénier S,Noirez P,Randrianarison-Huetz V,Cynober L,Aussel C,Moinard C

更新日期：2013-07-01 00:00:00

abstract：:Familial hemiplegic migraine type 1 (FHM1) is caused by missense mutations in the CACNA1A gene that encodes the alpha1A pore-forming subunit of Ca(V)2.1 Ca(2+) channels. Knock-in (KI) transgenic mice expressing Ca(V)2.1 Ca(2+) channels with a human pathogenic FHM1 mutation reveal enhanced glutamatergic neurotransmissi...

journal_title：Proteomics

authors： Klychnikov OI,Li KW,Sidorov IA,Loos M,Spijker S,Broos LA,Frants RR,Ferrari MD,Mayboroda OA,Deelder AM,Smit AB,van den Maagdenberg AM

更新日期：2010-07-01 00:00:00

abstract：:The basic problem of complexity poses a significant challenge for proteomic studies. To date two-dimensional gel electrophoresis (2-DE) followed by enzymatic in-gel digestion of the peptides, and subsequent identification by mass spectrometry (MS) is the most commonly used method to analyze complex protein mixtures. H...

journal_title：Proteomics

authors： Ihling C,Sinz A

更新日期：2005-05-01 00:00:00

abstract：:Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient se...

journal_title：Proteomics

authors： Shi T,Su D,Liu T,Tang K,Camp DG 2nd,Qian WJ,Smith RD

更新日期：2012-04-01 00:00:00

abstract：:Egr1 (Zif268) is an immediate early gene encoding an inducible transcription factor involved in synaptic plasticity and several forms of memory in rodents. Using 2-DE and MS, we compared proteomes of hippocampal subregions and cortex in Egr1-deficient and wild-type littermates. Two significant differences were identif...

journal_title：Proteomics

authors： Chardonnet S,Decottignies P,Amar L,Le Caer JP,Davis S,Laroche S,Le Maréchal P

更新日期：2007-01-01 00:00:00

abstract：:The community working on model organisms is growing steadily and the number of model organisms for which proteome data are being generated is continuously increasing. To standardize efforts and to make optimal use of proteomics data acquired from model organisms, a new Human Proteome Organisation (HUPO) initiative on ...

journal_title：Proteomics

authors： Jones AM,Aebersold R,Ahrens CH,Apweiler R,Baerenfaller K,Baker M,Bendixen E,Briggs S,Brownridge P,Brunner E,Daube M,Deutsch EW,Grossniklaus U,Heazlewood J,Hengartner MO,Hermjakob H,Jovanovic M,Lawless C,Lochnit G,Ma

更新日期：2012-02-01 00:00:00

abstract：:Secretory proteins of Mycobacterium tuberculosis have created more concern, given their dominant immunogenicity and role in pathogenesis. In view of expensive and time-consuming traditional biochemical experiments, an advanced support vector machine model named SecProMTB is constructed in this study and the proteins a...

journal_title：Proteomics

authors： Meng C,Wei L,Zou Q

更新日期：2019-09-01 00:00:00

abstract：:Identification of mucin-type O-glycosylated proteins with known functions in model organisms like Drosophila could provide keys to elucidate functions of the O-glycan moiety and proteomic analyses of O-glycoproteins in higher eukaryotes remain a challenge due to structural heterogeneity and a lack of efficient tools f...

journal_title：Proteomics

authors： Schwientek T,Mandel U,Roth U,Müller S,Hanisch FG

更新日期：2007-09-01 00:00:00

abstract：:CREC proteins constitute a family of EF-hand calcium binding proteins localized to the secretory pathway. Calumenin is the only member known to be secreted. Recently, it was shown that thrombin-activated thrombocytes liberate calumenin, which also is found in atherosclerotic lesions but not in normal vasculature. To s...

journal_title：Proteomics

authors： Ostergaard M,Hansen GA,Vorum H,Honoré B

更新日期：2006-06-01 00:00:00

abstract：:As demonstrated in this study, a CIEF-based multidimensional separation platform not only is compatible with the detergent-based membrane protein preparation protocol, but also achieves both the largest yeast membrane proteome coverage and the most comprehensive analysis of the yeast proteome to date. By using a 1% fa...

journal_title：Proteomics

authors： Wang W,Guo T,Song T,Lee CS,Balgley BM

更新日期：2007-04-01 00:00:00

abstract：:We describe the first phosphoproteome of the model filamentous fungus Aspergillus nidulans. Phosphopeptides were enriched using titanium dioxide, separated using a convenient ultra-long reverse phase gradient, and identified using a "high-high" strategy (high mass accuracy on the parent and fragment ions) with higher-...

journal_title：Proteomics

authors： Ramsubramaniam N,Harris SD,Marten MR

更新日期：2014-11-01 00:00:00

abstract：:Proteins extracted from leaf blades of rice plants infected with blast fungus, Magnaporthe grisea, were separated by two-dimensional polyacrylamide gel electrophoresis. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane, and 63 proteins were analyzed by a gas-phase protein sequencer. ...

journal_title：Proteomics

authors： Konishi H,Ishiguro K,Komatsu S

更新日期：2001-09-01 00:00:00

abstract：:Collagen-related peptide (CRP) stimulates powerful activation of platelets through the glycoprotein VI (GPVI)-FcR gamma-chain complex. We have combined proteomics and traditional biochemistry approaches to study the proteome of CRP-activated platelets, focusing in detail on tyrosine phosphorylation. In two separate ap...

journal_title：Proteomics

authors： García A,Senis YA,Antrobus R,Hughes CE,Dwek RA,Watson SP,Zitzmann N

更新日期：2006-10-01 00:00:00

abstract：:Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk-secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins ...

journal_title：Proteomics

authors： Janjanam J,Jamwal M,Singh S,Kumar S,Panigrahi AK,Hariprasad G,Jena MK,Anand V,Kumar S,Kaushik JK,Dang AK,Mukesh M,Mishra BP,Srinivasan A,Reddy VS,Mohanty AK

更新日期：2013-11-01 00:00:00

abstract：:The aim of the study was to evaluate the SELDI-TOF MS technique for pancreatic islet research. Mouse islets were cultured at low or high glucose levels in the absence or presence of oleate and characterized by measuring insulin secretion and oxygen tension. Subsequently, the islets were protein profiled. Up to 200 dif...

journal_title：Proteomics

authors： Ortsäter H,Sundsten T,Lin JM,Bergsten P

更新日期：2007-09-01 00:00:00

abstract：:The nucleus is the organelle where basically all DNA-related processes take place in eukaryotes, such as replication, transcription, and splicing as well as epigenetic regulation. The identification and description of the nuclear proteins is one of the requisites toward a comprehensive understanding of the biological ...

journal_title：Proteomics

authors： Bigeard J,Rayapuram N,Bonhomme L,Hirt H,Pflieger D

更新日期：2014-10-01 00:00:00

abstract：:To date, proteomics approaches have aimed to either identify novel proteins or change in protein expression/modification in various organisms under normal or disease conditions. One major aspect of functional proteomics is to identify protein biological properties in a given context, however, forward proteomics approa...

journal_title：Proteomics

authors： Palcy S,Chevet E

更新日期：2006-10-01 00:00:00

abstract：:In this work, for the first time, perfluorinated magnetic mesoporous microspheres were designed and synthesized for the highly specific enrichment of fluorous-derivatized phosphopeptides through the unique fluorine-fluorine interactions. The perfluorinated magnetic mesoporous microspheres were prepared through a surfa...

journal_title：Proteomics

authors： Zhao M,Deng C

更新日期：2016-04-01 00:00:00

abstract：:The International Protein Index (IPI) database has been one of the most widely used protein databases in MS proteomics approaches. Recently, the closure of IPI in September 2011 was announced. Its recommended replacement is the new UniProt Knowledgebase (UniProtKB) "complete proteome" sets, launched in May 2011. Here,...

journal_title：Proteomics

authors： Griss J,Martín M,O'Donovan C,Apweiler R,Hermjakob H,Vizcaíno JA

更新日期：2011-11-01 00:00:00

abstract：:The array of biomolecules generated by a functioning ecosystem represents both a potential resource for sustainable harvest and a potential indicator of ecosystem health and function. The cupped leaves of the carnivorous pitcher plant, Sarracenia purpurea, harbor a dynamic food web of aquatic invertebrates in a fully ...

journal_title：Proteomics

authors： Gotelli NJ,Smith AM,Ellison AM,Ballif BA

更新日期：2011-06-01 00:00:00

abstract：:Colorectal cancer risk is increased when dietary folate intake is low, with or without a deficiency in methylenetetrahydrofolate reductase (MTHFR). We have observed that intestinal tumors are induced in mice fed low-folate diets, and that tumor incidence is increased when these mice also have MTHFR deficiency. This st...

journal_title：Proteomics

authors： Leclerc D,Dejgaard K,Mazur A,Deng L,Wu Q,Nilsson T,Rozen R

更新日期：2014-11-01 00:00:00

abstract：:Protein-ligand interactions serve as fundamental regulators of numerous biological processes. Among protein-ligand pairs, glycan binding proteins (GBPs) and the glycans they recognize represent unique and highly complex interactions implicated in a broad range of regulatory activities. With few exceptions, cell surfac...

journal_title：Proteomics

authors： Kamili NA,Arthur CM,Gerner-Smidt C,Tafesse E,Blenda A,Dias-Baruffi M,Stowell SR

更新日期：2016-12-01 00:00:00

abstract：:The hair follicle dermal papilla is composed primarily of extracellular matrix (ECM) proteins secreted by resident fibroblasts. Dermal papilla is endowed with hair morphogenic properties, yet its composition is poorly characterized. In an attempt to understand its specificity better, we compared the protein compositio...

journal_title：Proteomics

authors： Pflieger D,Chabane S,Gaillard O,Bernard BA,Ducoroy P,Rossier J,Vinh J

更新日期：2006-11-01 00:00:00

abstract：:Geobacter sulfurreducens, generally considered to be a strict anaerobe, is a predominant microbe in subsurface environments, where it utilizes available metals as electron acceptors. To better understand the metabolic processes involved in the metal-reduction capability of this microbe, the proteins expressed by cells...

journal_title：Proteomics

authors： Khare T,Esteve-Núñez A,Nevin KP,Zhu W,Yates JR 3rd,Lovley D,Giometti CS

更新日期：2006-01-01 00:00:00

abstract：:Recombinant human growth hormone (rhGH) is used for the treatment of several disorders. Structural integrity of rhGH is of critical importance for its clinical use and modifications thereof may act as markers in situations such as rhGH doping, as illegal rhGH-abuse in sports is of increasing interest. In the current s...

journal_title：Proteomics

authors： Hepner F,Csaszar E,Roitinger E,Pollak A,Lubec G

更新日期：2006-02-01 00:00:00

abstract：:When the p-value is set at <0.05 in statistical group comparisons, a 5% rate of "false significant" results is expected. In order to test the reliability of our 2-DE method, we loaded each of 24 gels with equal-sized samples (200 mug protein from pooled rat brain, pH 4-7, stained with ruthenium fluorescent stain for v...

journal_title：Proteomics

authors： Fuxius S,Eravci M,Broedel O,Weist S,Mansmann U,Eravci S,Baumgartner A

更新日期：2008-05-01 00:00:00

abstract：:In order to discover novel protein markers indicative of disease processes or drug effects, the proteomics technology platform most commonly used consists of high resolution protein separation by two-dimensional electrophoresis (2-DE), mass spectrometric identification of proteins from stained gel spots and a bioinfor...

journal_title：Proteomics

authors： Pieper R,Su Q,Gatlin CL,Huang ST,Anderson NL,Steiner S

更新日期：2003-04-01 00:00:00

abstract：:In a comparative study, we investigated the influence of nine sample preparation workflows and seven different lysis buffers for qualitative and quantitative analysis of the human adipose tissue proteome. Adipose tissue is not just a fat depot but also an endocrine organ, which cross-talks with other tissue types and ...

journal_title：Proteomics

authors： Pasing Y,Colnoe S,Hansen T

更新日期：2017-03-01 00:00:00

abstract：:Treatment of patients with malignant melanoma with interferon-alpha achieves a response in a small but significant subset of patients. Currently, although much is known about interferon biology, little is known about either the particular mechanisms of interferon-alpha activity that are crucial for response or why onl...

journal_title：Proteomics

authors： Craven RA,Stanley AJ,Hanrahan S,Totty N,Jackson DP,Popescu R,Taylor A,Frey J,Selby PJ,Patel PM,Banks RE

更新日期：2004-12-01 00:00:00

abstract：:Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage ...

journal_title：Proteomics

authors： Franco CF,Santos R,Coelho AV

更新日期：2011-09-01 00:00:00