Proteomic analysis in human breast cancer: identification of a characteristic protein expression profile of malignant breast epithelium.

abstract：:Two-dimensional electrophoresis is a widely used method for separating a large number of proteins from complex protein mixtures and for revealing differential patterns of protein expressions. In the computer-assisted proteome research, the comparison of protein separation profiles involves several heuristic steps, ran...

journal_title：Proteomics

authors： Salmi J,Aittokallio T,Westerholm J,Griese M,Rosengren A,Nyman TA,Lahesmaa R,Nevalainen O

更新日期：2002-11-01 00:00:00

abstract：:Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...

journal_title：Proteomics

authors： Ahmed N,Barker G,Oliva K,Garfin D,Talmadge K,Georgiou H,Quinn M,Rice G

更新日期：2003-10-01 00:00:00

abstract：:Anaeromyxobacter dehalogenans is a microaerophilic member of the delta-proteobacteria which is able to utilize a wide range of electron acceptors, including halogenated phenols, U(VI), Fe(III), nitrate, nitrite, oxygen and fumarate. To date, the knowledge regarding general metabolic activities of this ecologically rel...

journal_title：Proteomics

authors： Chao TC,Kalinowski J,Nyalwidhe J,Hansmeier N

更新日期：2010-04-01 00:00:00

abstract：:In recent years, proteomics research has gained importance due to increasingly powerful techniques in protein purification, mass spectrometry and identification, and due to the development of extensive protein and DNA databases from various organisms. Nevertheless, current identification methods from spectrometric dat...

journal_title：Proteomics

authors： Hernandez P,Gras R,Frey J,Appel RD

更新日期：2003-06-01 00:00:00

abstract：:Among the group of lactic acid bacteria, Streptococcus thermophilus has found a wide application in industrial processes used for the manufacture of dairy products. Taking advantage of different proteome extraction and subfractionation protocols, bacterial cytosolic and membrane proteins were isolated and resolved by ...

journal_title：Proteomics

authors： Salzano AM,Arena S,Renzone G,D'Ambrosio C,Rullo R,Bruschi M,Ledda L,Maglione G,Candiano G,Ferrara L,Scaloni A

更新日期：2007-05-01 00:00:00

abstract：:Helicobacter pylori is one of the most common bacterial pathogens and causes a variety of diseases, such as peptic ulcer or gastric cancer. Despite intensive study of this human pathogen in the last decades, knowledge about its membrane proteins and, in particular, those which are putative components of the type IV se...

journal_title：Proteomics

authors： Backert S,Kwok T,Schmid M,Selbach M,Moese S,Peek RM Jr,König W,Meyer TF,Jungblut PR

更新日期：2005-04-01 00:00:00

abstract：:Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. sc...

journal_title：Proteomics

authors： Barnabas L,Ashwin NM,Kaverinathan K,Trentin AR,Pivato M,Sundar AR,Malathi P,Viswanathan R,Rosana OB,Neethukrishna K,Carletti P,Arrigoni G,Masi A,Agrawal GK,Rakwal R

更新日期：2016-04-01 00:00:00

abstract：:2-D analysis of plant proteomes containing thousands of proteins has limited dynamic resolution because only abundant proteins can be detected. Proteomic assessment of the non-abundant proteins within seeds is difficult when 60-80% is storage proteins. Resolution can be improved through sample fractionation using sepa...

journal_title：Proteomics

authors： Krishnan HB,Oehrle NW,Natarajan SS

更新日期：2009-06-01 00:00:00

abstract：:Most proteins execute their functions through interacting with other proteins. Thus, understanding protein-protein interactions (PPIs) is essential to decipher biological functions in a living cell. To predict large-scale PPIs, effective and efficient computational approaches are desirable to integrate heterogeneous d...

journal_title：Proteomics

authors： Lin X,Chen XW

更新日期：2013-01-01 00:00:00

abstract：:In this work, we first immobilized tin(IV) ion on polydopamine-coated magnetic graphene (magG@PDA) to synthesize Sn4+ -immobilized magG@PDA (magG@PDA-Sn4+ ) and successfully applied the material to highly selective enrichment of phosphopeptides. The material gathered the advantages of large surface area of graphene, s...

journal_title：Proteomics

authors： Lin H,Deng C

更新日期：2016-11-01 00:00:00

abstract：:Advances in gel-based nonradioactive protein expression and PTM detection using fluorophores has served as the impetus for developing analytical instrumentation with improved imaging capabilities. We describe a CCD camera-based imaging instrument, equipped with both a high-pressure Xenon arc lamp and a UV transillumin...

journal_title：Proteomics

authors： Scrivener E,Boghigian BA,Golenko E,Bogdanova A,Jackson P,Mikulskis A,Denoyer E,Courtney P,Lopez MF,Patton WF

更新日期：2005-11-01 00:00:00

abstract：:Recombinant human growth hormone (rhGH) is used for the treatment of several disorders. Structural integrity of rhGH is of critical importance for its clinical use and modifications thereof may act as markers in situations such as rhGH doping, as illegal rhGH-abuse in sports is of increasing interest. In the current s...

journal_title：Proteomics

authors： Hepner F,Csaszar E,Roitinger E,Pollak A,Lubec G

更新日期：2006-02-01 00:00:00

abstract：:Following transmission to the vertebrate host, the protozoan parasite Leishmania donovani differentiates into the pathogenic amastigote stage that is adapted for intracellular survival. This developmental transition is induced by environmental factors including elevated temperature and acidic pH and is likely transduc...

journal_title：Proteomics

authors： Morales MA,Watanabe R,Laurent C,Lenormand P,Rousselle JC,Namane A,Späth GF

更新日期：2008-01-01 00:00:00

abstract：:Interleukin (IL)-6 is a circulatory, pleiotropic cytokine with multiple roles in the immune system. Both IL-6 and the IL6 -174G>C promoter polymorphism have been linked to various diseases associated with inflammation. However, the mechanism by which the polymorphism influences disease risk is unclear. We postulated t...

journal_title：Proteomics

authors： Hegedus CM,Skibola CF,Bracci P,Holly EA,Smith MT

更新日期：2007-02-01 00:00:00

abstract：:Ubiquitin-dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two-hybrid analysis of their interactions with each other. We observed that there are 114 interacting-pairs ...

journal_title：Proteomics

authors： Chen C,Huang C,Chen S,Liang J,Lin W,Ke G,Zhang H,Wang B,Huang J,Han Z,Ma L,Huo K,Yang X,Yang P,He F,Tao T

更新日期：2008-02-01 00:00:00

abstract：:Quantitative proteomics technology based on isobaric tags is playing an important role in proteomic investigation. In this paper, we present an automated software, named IQuant, which integrates a postprocessing tool of protein identification and advanced statistical algorithms to process the MS/MS signals generated f...

journal_title：Proteomics

authors： Wen B,Zhou R,Feng Q,Wang Q,Wang J,Liu S

更新日期：2014-10-01 00:00:00

abstract：:Over the last decade, an increasing prevalence of peanut allergies was observed worldwide. Peanuts are meanwhile categorized among the most dangerous food allergens. This is particularly relevant since peanut-derived ingredients are widely used in industrial food production. To minimize the problem of hidden food alle...

journal_title：Proteomics

authors： Schmidt H,Gelhaus C,Latendorf T,Nebendahl M,Petersen A,Krause S,Leippe M,Becker WM,Janssen O

更新日期：2009-07-01 00:00:00

abstract：:Realistic in vitro models are critical in the drug development process. In this study, a novel in vitro platform is employed to assess drug penetration and metabolism. This platform, which utilizes a 3D printed fluidic device, allows for dynamic dosing of three dimensional cell cultures, also known as spheroids. The p...

journal_title：Proteomics

authors： LaBonia GJ,Lockwood SY,Heller AA,Spence DM,Hummon AB

更新日期：2016-06-01 00:00:00

abstract：:Protein-ligand interactions serve as fundamental regulators of numerous biological processes. Among protein-ligand pairs, glycan binding proteins (GBPs) and the glycans they recognize represent unique and highly complex interactions implicated in a broad range of regulatory activities. With few exceptions, cell surfac...

journal_title：Proteomics

authors： Kamili NA,Arthur CM,Gerner-Smidt C,Tafesse E,Blenda A,Dias-Baruffi M,Stowell SR

更新日期：2016-12-01 00:00:00

abstract：:Sample preparation is a critical step in the proteomic workflow. Numerous different approaches are used, tailored to the type of sample, the aims of the experiment, analytical method, and to an extent, user preference. This has resulted in large variation in reported protein abundances. In this study, the complementar...

journal_title：Proteomics

authors： Howard M,Achour B,Al-Majdoub Z,Rostami-Hodjegan A,Barber J

更新日期：2018-12-01 00:00:00

abstract：:Plant cell walls (CWs) contain a large proportion of polysaccharides (90-95% of CW mass) and proteins (5-10%) that play major roles in CW plasticity during development and in response to environmental cues. Here, we present CW proteomics data of Arabidopsis thaliana roots. Plants were cultivated in hydroponic conditio...

journal_title：Proteomics

authors： Nguyen-Kim H,San Clemente H,Balliau T,Zivy M,Dunand C,Albenne C,Jamet E

更新日期：2016-02-01 00:00:00

abstract：:We have developed a surface plasmon resonance (SPR)-based protein microarray to study protein-protein interactions in a high-throughput mode. As a model system, triple protein interactions have been explored with human papillomaviral E6 protein, tumor suppressor p53, and ubiquitin ligase E6AP. Human papillomavirus (HP...

journal_title：Proteomics

authors： Ro HS,Koh BH,Jung SO,Park HK,Shin YB,Kim MG,Chung BH

更新日期：2006-04-01 00:00:00

abstract：:The large interactive surfaces of nanoparticles (NPs) increase the opportunities to develop NPs for vascular targeting. Proteomic analysis of endothelial cells exposed to NPs reveals the cellular response and turns the focus into the impairment of the endothelial permeability. Here, quantitative proteomics and transcr...

journal_title：Proteomics

authors： Kuruvilla J,Bayat N,Cristobal S

更新日期：2019-03-01 00:00:00

abstract：:The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis proce...

journal_title：Proteomics

authors： Journet A,Klein G,Brugière S,Vandenbrouck Y,Chapel A,Kieffer S,Bruley C,Masselon C,Aubry L

更新日期：2012-01-01 00:00:00

abstract：:The proteins recovered in exhaled breath condensate (EBC) might be used to non-invasively monitor respiratory diseases. However, the range of proteins and their source are still unresolved and contamination by saliva or a similar protein pattern in the nasal and bronchial compartments may make interpretation of the da...

journal_title：Proteomics

authors： Griese M,Noss J,von Bredow C

更新日期：2002-06-01 00:00:00

abstract：:Experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. Channel catfish herpesvirus (CCV) is a simple comparative model for understanding herp...

journal_title：Proteomics

authors： Kunec D,Nanduri B,Burgess SC

更新日期：2009-05-01 00:00:00

abstract：:Extracellular vesicles (EVs) are lipid-bilayered vesicles that are released by multiple cell types and contain nucleic acids and proteins. Very little is known about how the cargo is packaged into EVs. Ubiquitination of proteins is a key posttranslational modification that regulates protein stability and trafficking t...

journal_title：Proteomics

authors： Anand S,Foot N,Ang CS,Gembus KM,Keerthikumar S,Adda CG,Mathivanan S,Kumar S

更新日期：2018-09-01 00:00:00

abstract：:Intracellular serine protease-1 (Isp-1) is a major intracellular serine protease of Bacillus subtilis, whose functions still remain largely unknown. Furthermore, physiological substrates are yet to be determined. To identify Isp-1 substrates, we digested extract obtained from an Isp-1 deficient Bacillus mutant with pu...

journal_title：Proteomics

authors： Lee AY,Goo Park S,Kho CW,Young Park S,Cho S,Lee SC,Lee DH,Myung PK,Park BC

更新日期：2004-11-01 00:00:00

abstract：:Long-lived conifer trees depend on both constitutive and induced defenses for resistance against a myriad of potential pathogens and herbivores. In species of spruce (Picea spp.), several of the late events of pathogen-, insect-, or elicitor-induced defense responses have previously been characterized at the anatomica...

journal_title：Proteomics

authors： Lippert DN,Ralph SG,Phillips M,White R,Smith D,Hardie D,Gershenzon J,Ritland K,Borchers CH,Bohlmann J

更新日期：2009-01-01 00:00:00

abstract：:The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was...

journal_title：Proteomics

authors： Sinnige JC,de Been M,Zhou M,Bonten MJ,Willems RJ,Top J

更新日期：2015-11-01 00:00:00