Proteomic analysis in human breast cancer: identification of a characteristic protein expression profile of malignant breast epithelium.


:Gene expression analysis has become a promising tool in predicting the clinical course of malignant disease and the response to antineoplastic therapy. Surprisingly, only little is known about the protein expression pattern of human tumors. Recent advances in proteomic analysis allow proteins of interest to be identified by their expression and/or modification pattern in 2-DE rather than using the traditional approach of translating gene expression data. To identify a proteomic pattern that is characteristic for malignant breast epithelium, we performed differential 2-DE analysis in sets of microdissected malignant breast epithelia and corresponding adjacent normal breast epithelia from five patients with invasive breast carcinoma. Thirty-two protein spots were found to be selectively regulated in malignant epithelium, and were subjected to MALDI-TOF and/or immunoblotting for protein identification. Thirteen of the identified proteins had previously not been associated with breast cancer. The validity of these findings was confirmed by literature review and immunohistochemistry for identified proteins in an independent cohort of 50 breast cancer specimens. We here describe, for the first time, a proteomic analysis of matched normal and malignant epithelia from invasive breast carcinomas. This strategy leads to a better understanding of oncogenesis at an operational level and helps to characterize the malignant phenotype of individual tumors, and thereby to identify novel targets for antineoplastic therapy.






Hudelist G,Singer CF,Pischinger KI,Kaserer K,Manavi M,Kubista E,Czerwenka KF




Has Abstract


2006-03-01 00:00:00












  • Hierarchical grid transformation for image warping in the analysis of two-dimensional electrophoresis gels.

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    pub_type: 杂志文章


    authors: Salmi J,Aittokallio T,Westerholm J,Griese M,Rosengren A,Nyman TA,Lahesmaa R,Nevalainen O

    更新日期:2002-11-01 00:00:00

  • An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum.

    abstract::Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute appro...


    pub_type: 杂志文章


    authors: Ahmed N,Barker G,Oliva K,Garfin D,Talmadge K,Georgiou H,Quinn M,Rice G

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  • Comprehensive proteome profiling of the Fe(III)-reducing myxobacterium Anaeromyxobacter dehalogenans 2CP-C during growth with fumarate and ferric citrate.

    abstract::Anaeromyxobacter dehalogenans is a microaerophilic member of the delta-proteobacteria which is able to utilize a wide range of electron acceptors, including halogenated phenols, U(VI), Fe(III), nitrate, nitrite, oxygen and fumarate. To date, the knowledge regarding general metabolic activities of this ecologically rel...


    pub_type: 杂志文章


    authors: Chao TC,Kalinowski J,Nyalwidhe J,Hansmeier N

    更新日期:2010-04-01 00:00:00

  • Popitam: towards new heuristic strategies to improve protein identification from tandem mass spectrometry data.

    abstract::In recent years, proteomics research has gained importance due to increasingly powerful techniques in protein purification, mass spectrometry and identification, and due to the development of extensive protein and DNA databases from various organisms. Nevertheless, current identification methods from spectrometric dat...


    pub_type: 杂志文章


    authors: Hernandez P,Gras R,Frey J,Appel RD

    更新日期:2003-06-01 00:00:00

  • A widespread picture of the Streptococcus thermophilus proteome by cell lysate fractionation and gel-based/gel-free approaches.

    abstract::Among the group of lactic acid bacteria, Streptococcus thermophilus has found a wide application in industrial processes used for the manufacture of dairy products. Taking advantage of different proteome extraction and subfractionation protocols, bacterial cytosolic and membrane proteins were isolated and resolved by ...


    pub_type: 杂志文章


    authors: Salzano AM,Arena S,Renzone G,D'Ambrosio C,Rullo R,Bruschi M,Ledda L,Maglione G,Candiano G,Ferrara L,Scaloni A

    更新日期:2007-05-01 00:00:00

  • Subproteomes of soluble and structure-bound Helicobacter pylori proteins analyzed by two-dimensional gel electrophoresis and mass spectrometry.

    abstract::Helicobacter pylori is one of the most common bacterial pathogens and causes a variety of diseases, such as peptic ulcer or gastric cancer. Despite intensive study of this human pathogen in the last decades, knowledge about its membrane proteins and, in particular, those which are putative components of the type IV se...


    pub_type: 杂志文章


    authors: Backert S,Kwok T,Schmid M,Selbach M,Moese S,Peek RM Jr,König W,Meyer TF,Jungblut PR

    更新日期:2005-04-01 00:00:00

  • Proteomic analysis of a compatible interaction between sugarcane and Sporisorium scitamineum.

    abstract::Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. sc...


    pub_type: 杂志文章


    authors: Barnabas L,Ashwin NM,Kaverinathan K,Trentin AR,Pivato M,Sundar AR,Malathi P,Viswanathan R,Rosana OB,Neethukrishna K,Carletti P,Arrigoni G,Masi A,Agrawal GK,Rakwal R

    更新日期:2016-04-01 00:00:00

  • A rapid and simple procedure for the depletion of abundant storage proteins from legume seeds to advance proteome analysis: a case study using Glycine max.

    abstract::2-D analysis of plant proteomes containing thousands of proteins has limited dynamic resolution because only abundant proteins can be detected. Proteomic assessment of the non-abundant proteins within seeds is difficult when 60-80% is storage proteins. Resolution can be improved through sample fractionation using sepa...


    pub_type: 杂志文章


    authors: Krishnan HB,Oehrle NW,Natarajan SS

    更新日期:2009-06-01 00:00:00

  • Heterogeneous data integration by tree-augmented naïve Bayes for protein-protein interactions prediction.

    abstract::Most proteins execute their functions through interacting with other proteins. Thus, understanding protein-protein interactions (PPIs) is essential to decipher biological functions in a living cell. To predict large-scale PPIs, effective and efficient computational approaches are desirable to integrate heterogeneous d...


    pub_type: 杂志文章


    authors: Lin X,Chen XW

    更新日期:2013-01-01 00:00:00

  • Development of immobilized Sn4+ affinity chromatography material for highly selective enrichment of phosphopeptides.

    abstract::In this work, we first immobilized tin(IV) ion on polydopamine-coated magnetic graphene (magG@PDA) to synthesize Sn4+ -immobilized magG@PDA (magG@PDA-Sn4+ ) and successfully applied the material to highly selective enrichment of phosphopeptides. The material gathered the advantages of large surface area of graphene, s...


    pub_type: 杂志文章


    authors: Lin H,Deng C

    更新日期:2016-11-01 00:00:00

  • Performance validation of an improved Xenon-arc lamp-based CCD camera system for multispectral imaging in proteomics.

    abstract::Advances in gel-based nonradioactive protein expression and PTM detection using fluorophores has served as the impetus for developing analytical instrumentation with improved imaging capabilities. We describe a CCD camera-based imaging instrument, equipped with both a high-pressure Xenon arc lamp and a UV transillumin...


    pub_type: 杂志文章


    authors: Scrivener E,Boghigian BA,Golenko E,Bogdanova A,Jackson P,Mikulskis A,Denoyer E,Courtney P,Lopez MF,Patton WF

    更新日期:2005-11-01 00:00:00

  • Massspectrometrical analysis of recombinant human growth hormone Norditropin reveals amino acid exchange at M14_V14 rhGH.

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    pub_type: 杂志文章


    authors: Hepner F,Csaszar E,Roitinger E,Pollak A,Lubec G

    更新日期:2006-02-01 00:00:00

  • Phosphoproteomic analysis of Leishmania donovani pro- and amastigote stages.

    abstract::Following transmission to the vertebrate host, the protozoan parasite Leishmania donovani differentiates into the pathogenic amastigote stage that is adapted for intracellular survival. This developmental transition is induced by environmental factors including elevated temperature and acidic pH and is likely transduc...


    pub_type: 杂志文章


    authors: Morales MA,Watanabe R,Laurent C,Lenormand P,Rousselle JC,Namane A,Späth GF

    更新日期:2008-01-01 00:00:00

  • Screening the human serum proteome for genotype-phenotype associations: an analysis of the IL6 -174G>C polymorphism.

    abstract::Interleukin (IL)-6 is a circulatory, pleiotropic cytokine with multiple roles in the immune system. Both IL-6 and the IL6 -174G>C promoter polymorphism have been linked to various diseases associated with inflammation. However, the mechanism by which the polymorphism influences disease risk is unclear. We postulated t...


    pub_type: 杂志文章


    authors: Hegedus CM,Skibola CF,Bracci P,Holly EA,Smith MT

    更新日期:2007-02-01 00:00:00

  • Subunit-subunit interactions in the human 26S proteasome.

    abstract::Ubiquitin-dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two-hybrid analysis of their interactions with each other. We observed that there are 114 interacting-pairs ...


    pub_type: 杂志文章


    authors: Chen C,Huang C,Chen S,Liang J,Lin W,Ke G,Zhang H,Wang B,Huang J,Han Z,Ma L,Huo K,Yang X,Yang P,He F,Tao T

    更新日期:2008-02-01 00:00:00

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    pub_type: 杂志文章


    authors: Wen B,Zhou R,Feng Q,Wang Q,Wang J,Liu S

    更新日期:2014-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Schmidt H,Gelhaus C,Latendorf T,Nebendahl M,Petersen A,Krause S,Leippe M,Becker WM,Janssen O

    更新日期:2009-07-01 00:00:00

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    pub_type: 杂志文章


    authors: LaBonia GJ,Lockwood SY,Heller AA,Spence DM,Hummon AB

    更新日期:2016-06-01 00:00:00

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    abstract::Protein-ligand interactions serve as fundamental regulators of numerous biological processes. Among protein-ligand pairs, glycan binding proteins (GBPs) and the glycans they recognize represent unique and highly complex interactions implicated in a broad range of regulatory activities. With few exceptions, cell surfac...


    pub_type: 杂志文章,评审


    authors: Kamili NA,Arthur CM,Gerner-Smidt C,Tafesse E,Blenda A,Dias-Baruffi M,Stowell SR

    更新日期:2016-12-01 00:00:00

  • GASP and FASP are Complementary for LC-MS/MS Proteomic Analysis of Drug-Metabolizing Enzymes and Transporters in Pig Liver.

    abstract::Sample preparation is a critical step in the proteomic workflow. Numerous different approaches are used, tailored to the type of sample, the aims of the experiment, analytical method, and to an extent, user preference. This has resulted in large variation in reported protein abundances. In this study, the complementar...


    pub_type: 杂志文章


    authors: Howard M,Achour B,Al-Majdoub Z,Rostami-Hodjegan A,Barber J

    更新日期:2018-12-01 00:00:00

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    pub_type: 杂志文章


    authors: Nguyen-Kim H,San Clemente H,Balliau T,Zivy M,Dunand C,Albenne C,Jamet E

    更新日期:2016-02-01 00:00:00

  • Surface plasmon resonance imaging protein arrays for analysis of triple protein interactions of HPV, E6, E6AP, and p53.

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    pub_type: 杂志文章


    authors: Ro HS,Koh BH,Jung SO,Park HK,Shin YB,Kim MG,Chung BH

    更新日期:2006-04-01 00:00:00

  • Proteomic Analysis of Endothelial Cells Exposed to Ultrasmall Nanoparticles Reveals Disruption in Paracellular and Transcellular Transport.

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    pub_type: 杂志文章


    authors: Kuruvilla J,Bayat N,Cristobal S

    更新日期:2019-03-01 00:00:00

  • Investigating the macropinocytic proteome of Dictyostelium amoebae by high-resolution mass spectrometry.

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    pub_type: 杂志文章


    authors: Journet A,Klein G,Brugière S,Vandenbrouck Y,Chapel A,Kieffer S,Bruley C,Masselon C,Aubry L

    更新日期:2012-01-01 00:00:00

  • Protein pattern of exhaled breath condensate and saliva.

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    pub_type: 杂志文章


    authors: Griese M,Noss J,von Bredow C

    更新日期:2002-06-01 00:00:00

  • Experimental annotation of channel catfish virus by probabilistic proteogenomic mapping.

    abstract::Experimental identification of expressed proteins by proteomics constitutes the most reliable approach to identify genomic location and structure of protein-coding genes and substantially complements computational genome annotation. Channel catfish herpesvirus (CCV) is a simple comparative model for understanding herp...


    pub_type: 杂志文章


    authors: Kunec D,Nanduri B,Burgess SC

    更新日期:2009-05-01 00:00:00

  • Arrestin-Domain Containing Protein 1 (Arrdc1) Regulates the Protein Cargo and Release of Extracellular Vesicles.

    abstract::Extracellular vesicles (EVs) are lipid-bilayered vesicles that are released by multiple cell types and contain nucleic acids and proteins. Very little is known about how the cargo is packaged into EVs. Ubiquitination of proteins is a key posttranslational modification that regulates protein stability and trafficking t...


    pub_type: 杂志文章


    authors: Anand S,Foot N,Ang CS,Gembus KM,Keerthikumar S,Adda CG,Mathivanan S,Kumar S

    更新日期:2018-09-01 00:00:00

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    abstract::Intracellular serine protease-1 (Isp-1) is a major intracellular serine protease of Bacillus subtilis, whose functions still remain largely unknown. Furthermore, physiological substrates are yet to be determined. To identify Isp-1 substrates, we digested extract obtained from an Isp-1 deficient Bacillus mutant with pu...


    pub_type: 杂志文章


    authors: Lee AY,Goo Park S,Kho CW,Young Park S,Cho S,Lee SC,Lee DH,Myung PK,Park BC

    更新日期:2004-11-01 00:00:00

  • Quantitative iTRAQ proteome and comparative transcriptome analysis of elicitor-induced Norway spruce (Picea abies) cells reveals elements of calcium signaling in the early conifer defense response.

    abstract::Long-lived conifer trees depend on both constitutive and induced defenses for resistance against a myriad of potential pathogens and herbivores. In species of spruce (Picea spp.), several of the late events of pathogen-, insect-, or elicitor-induced defense responses have previously been characterized at the anatomica...


    pub_type: 杂志文章


    authors: Lippert DN,Ralph SG,Phillips M,White R,Smith D,Hardie D,Gershenzon J,Ritland K,Borchers CH,Bohlmann J

    更新日期:2009-01-01 00:00:00

  • Growth condition-dependent cell surface proteome analysis of Enterococcus faecium.

    abstract::The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was...


    pub_type: 杂志文章


    authors: Sinnige JC,de Been M,Zhou M,Bonten MJ,Willems RJ,Top J

    更新日期:2015-11-01 00:00:00