Abstract:
:Recombinant human growth hormone (rhGH) is used for the treatment of several disorders. Structural integrity of rhGH is of critical importance for its clinical use and modifications thereof may act as markers in situations such as rhGH doping, as illegal rhGH-abuse in sports is of increasing interest. In the current study we investigated homogeneity of Norditropin, a recombinant human growth hormone frequently used in medicine, expressed in E. coli, strain MC1061. The most recent proteomics technologies including 2-DE, MALDI-MS followed by MALDI-MS/MS and LC-MS followed by LC-MS/MS were used for the characterisation of rhGH. MALDI-TOF-TOF and electrospray LC-MS analysis revealed one major protein with an average molecular mass of 22 126.0 Da and some additional minor components. Electrospray LC-MS/MS of the enzymatically digested Norditropin sample showed deamidation of N(12)N(149) and N(159), oxidation of M(14), M(125) and M(170) and one amino acid exchange V(14) for M(14) present in <1% of Norditropin. While deamidation and oxidation may be due to technical reasons, the single amino acid exchange may reflect infidelity of translation rather than codon usage and copy editing by E. coli.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Hepner F,Csaszar E,Roitinger E,Pollak A,Lubec Gdoi
10.1002/pmic.200500334subject
Has Abstractpub_date
2006-02-01 00:00:00pages
775-84issue
3eissn
1615-9853issn
1615-9861journal_volume
6pub_type
杂志文章相关文献
PROTEOMICS文献大全abstract::The glycan structures of the major and more than ten minor populated isoforms of antithrombin (AT) were determined after separation of the isoforms by IEF using IPG strips. The bands excised from the gel were reduced, derivatized by iodoacetamide and submitted to tryptic digestion. The digest was analyzed by RP-HPLC-E...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401238
更新日期:2005-10-01 00:00:00
abstract::Integral membrane proteins (IMPs) perform crucial cellular functions and are the primary targets for most pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains and their intimate association with lipids make them difficult to handle. Numerous proteomic platforms that include LC sepa...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800210
更新日期:2008-10-01 00:00:00
abstract::Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic F. oxysporum strains. In this study, F. oxysporum wild-type (WT) MSA 35, a biocontrol multi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900716
更新日期:2010-09-01 00:00:00
abstract::With advances in liquid chromatography coupled to tandem mass spectrometry technologies combined with the continued goals of biomarker discovery, clinical applications of established biomarkers, and integrating large multiomic datasets (i.e. "big data"), there remains an urgent need for robust tools to assess instrume...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201400373
更新日期:2015-03-01 00:00:00
abstract::Trichomonas vaginalis, a sexually transmitted parasite, has many cysteine proteinases (CPs); some are involved in trichomonal pathogenesis, express during infection, and antibodies against CPs have been detected in patient sera. The goal of this study was to identify the antigenic proteinases of T. vaginalis as potent...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900479
更新日期:2010-02-01 00:00:00
abstract::Many acute and chronic lung injuries are incurable and rank as the fourth leading cause of death globally. While stem cell treatment for lung injuries is a promising approach, there is growing evidence that the therapeutic efficacy of stem cells originates from secreted extracellular vesicles (EVs). Consequently, EVs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201800166
更新日期:2019-09-01 00:00:00
abstract::Tumor development and progression consist of a series of complex processes involving multiple changes in gene expression (Paolo et al. Physiol. Rev., 1993, 73, 161-195; Lance et al. Cell., 1991, 64, 327-336). Tumor cells acquire an invasive and metastatic phenotype that is the main cause of death for cancer patients. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401132
更新日期:2005-03-01 00:00:00
abstract::Phosphoproteomics can be defined as the comprehensive study of protein phosphorylation by identification of the phosphoproteins, exact mapping of the phosphorylation sites, quantification of phosphorylation, and eventually, revealing their biological function. Its place in today's research is vitally important to addr...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200600232
更新日期:2006-10-01 00:00:00
abstract::The sample preparation procedures established for Prorocentrum triestinum were adapted to cover both thecate and athecate dinoflagellates. Further, whether trichloroacetic acid (TCA) precipitation can be used to fix and preserve the harmful or nuisance species from local waters that they infest was tested. Optimized t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300548
更新日期:2004-01-01 00:00:00
abstract::Most information on molecular processes accompanying and driving adipocyte differentiation are derived from rodent models. Here, we provide a comprehensive analysis of combined transcriptomic and proteomic alterations during adipocyte differentiation in Simpson-Golabi-Behmel Syndrome (SGBS) cells. The SGBS cells are a...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201900405
更新日期:2020-05-08 00:00:00
abstract::Antibiotics disturb the physiological homeostasis of bacterial cells by interfering with essential cellular functions or structures. The bacterial proteome adjusts quickly in response to antibiotic challenge. This physiological response is specifically tailored to overcome the inflicted damage and, thus, closely linke...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100046
更新日期:2011-08-01 00:00:00
abstract::Antibodies that recognize PTMs of histones play a central role in epigenetic proteomic research. Modification-specific antibodies are employed in chromatin immunoprecipitation, for Western blotting and during the immunoprecipitation steps for MS-based global proteomic analyses. Knowledge about the antibodies' off-targ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200383
更新日期:2013-03-01 00:00:00
abstract::In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well-wat...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800158
更新日期:2009-05-01 00:00:00
abstract::Determination of protein-protein interactions is an important component in assigning function and discerning the biological relevance of proteins within a broader cellular context. In vitro protein-protein interaction methodologies, including affinity chromatography, coimmunoprecipitation, and newer approaches such as...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200500517
更新日期:2006-04-01 00:00:00
abstract::Bio-active peptides are involved in the regulation of most physiological processes in the body. Classical bio-active peptides (CBAPs) are cleaved from a larger precursor protein and stored in secretion vesicles from which they are released in the extracellular space. Recently, another non-classical type of bio-active ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700218
更新日期:2018-05-01 00:00:00
abstract::The proteomic profile of hypothalamus, a key organ of CNS, is explored here by employing two widely used MS techniques, i.e. HPLC/ESI-ion trap and HPLC/ESI-quadrupole-TOF MS. Strong cation exchange is used for the fractionation of peptides and protein search engine MASCOT is employed for data query. One hundred and th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201300073
更新日期:2013-08-01 00:00:00
abstract::The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse live...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000126
更新日期:2010-11-01 00:00:00
abstract::Proteome analysis by two-dimensional gel electrophoresis (2-DE) together with mass spectrometry was applied to screen acute phase response (APR)-related proteins with low molecular weight in loach skin following injury. Furthermore, Western blotting and function tests were applied to confirm the results obtained from ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300864
更新日期:2004-12-01 00:00:00
abstract::Staphylococcus aureus, a bacterial, food-borne pathogen of humans, can contaminate raw fruits and vegetables. While physical and chemical methods are available to control S. aureus, scientists are searching for inhibitory phytochemicals from plants. One promising compound from pomegranate is punicalagin, a natural ant...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201700461
更新日期:2018-05-01 00:00:00
abstract::Mycobacterium avium subsp. paratuberculosis has long been recognized as the causative agent of Johne's disease, a chronic inflammatory intestinal disease of sheep, cattle and other ruminants. Mycobacterial cells are extremely hardy, and proteomic analyses require the use of harsh conditions to effect their disruption....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300672
更新日期:2004-04-01 00:00:00
abstract::The last 30 years Enterococcus faecium has become an important nosocomial pathogen in hospitals worldwide. The aim of this study was to obtain insight in the cell surface proteome of E. faecium when grown in laboratory and clinically relevant conditions. Enterococcus faecium E1162, a clinical blood stream isolate, was...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500138
更新日期:2015-11-01 00:00:00
abstract::Many tumors arising from epithelial tissues produce mucins, which readily come into contact with infiltrating cells in cancer tissues. MUC2 mucins were purified from the conditioned medium of a colorectal cancer cell line, LS180 cells. It is known that in cancer patients, the number of dendritic cells (DCs) is reduced...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800039
更新日期:2008-08-01 00:00:00
abstract::Staphylococcus aureus is an opportunistic human pathogen, which can cause life-threatening disease. Proteome analyses of the bacterium can provide new insights into its pathophysiology and important facets of metabolic adaptation and, thus, aid the recognition of targets for intervention. However, the value of such pr...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500091
更新日期:2015-11-01 00:00:00
abstract::The accurate and precise absolute abundance of proteins can be determined using mass spectrometry by spiking the sample with stable isotope-labeled standards. In this study, we developed a strategy of hierarchical use of peptide-concatenated standards (PCSs) to quantify more proteins over a wider dynamic range. Multip...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201500414
更新日期:2016-05-01 00:00:00
abstract::Over the past decade, considerable progress has been made with respect to the analytical methods for analysis of glycans from biological sources. Regardless of the specific methods that are used, glycan analysis includes isolation, identification, and quantitation. Derivatization is indispensable to increase their ide...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201500266
更新日期:2016-01-01 00:00:00
abstract::Protein expression changes induced in thioglycolate-elicited peritoneal murine macrophages (M Phi) by infection with type III Group B Streptococcus (GBS) are described. Proteins from control M Phi and M Phi incubated 2 h with live or heat-inactivated GBS were separated by 2-DE. Proteins whose expression was significan...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900642
更新日期:2010-06-01 00:00:00
abstract::Ischemia/reperfusion (I/R) injury is a major consequence of a cardiovascular intervention. The study of changes of the left and right ventricle proteomes from hearts subjected to I/R may be a key to revealing the pathological mechanisms underlying I/R-induced heart contractile dysfunction. Isolated rat hearts were per...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100604
更新日期:2012-08-01 00:00:00
abstract::Lysine crotonylation (Kcr) is a recently discovered post-translational modification that potentially regulates multiple biological processes. With an objective to expand the available crotonylation datasets, LC-MS/MS is performed using mouse liver samples under normal physiological conditions to obtain in vivo crotony...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.202000049
更新日期:2020-10-01 00:00:00
abstract::Human embryonic stem cells (hESCs) have received considerable attention due to their therapeutic potential and usefulness in understanding early development and cell fate commitment. In order to appreciate the unique properties of these pluripotent, self-renewing cells, we have performed an in-depth multidimensional f...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400132
更新日期:2015-01-01 00:00:00
abstract::Roots, leaves, and intermediate sections between roots and leaves (ISRL) of wheat seedlings show different physiological functions at the protein level. We performed the first integrative proteomic analysis of different tissues of the drought-tolerant wheat cultivar Hanxuan 10 (HX-10) and drought-sensitive cultivar Ch...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201400179
更新日期:2015-05-01 00:00:00