SILAC Expands its Territory to the Pathogenic Yeast, Candida albicans.

Abstract:

:Quantitative proteomic analysis using stable isotope labeling with amino acids in cell culture (SILAC), as metabolic labeling with MS, has been used as an excellent technique to measure relative abundance change in proteins and post-transitional modifications. Since its development in 2002, SILAC has proven to have unique and specific advantage compared to other labeling methods such as Isobaric tags for relative and absolute quantitation (iTRAQ) and Tandem Mass Tag (TMT). However, SILAC has limitations in its application to human tissue/organ samples and some types of unicellular organisms that convert supplemented heavy amino acids to others. In this issue, Kaneva et al. (Proteomics 2018, 18, 1700278) introduces a new application of SILAC to a pathogen, which allows quantitative proteomics analysis to be performed without the need of arginine auxotrophs for SILAC experiment. In fungal pathogens, such as Candida albicans and other yeast family, arginine metabolism is one of the factors that helps pathogen escape host's defenses. This prevents arginine auxotrophs from being used in C. albicans research and limits SILAC-based MS method as a choice of quantitation. However, possibilities for quantitative proteomic analysis of a pathogenic yeast C. albicans using SILAC has now opened by Kaneva et al.

journal_name

Proteomics

journal_title

Proteomics

authors

Jang WE,Kim MS

doi

10.1002/pmic.201700458

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

e1700458

issue

5-6

eissn

1615-9853

issn

1615-9861

journal_volume

18

pub_type

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