Protein sequence tags: a novel solution for comparative proteomics.

abstract：:Due to the lack of precise markers indicative of its occurrence and progression, coronary artery disease (CAD), the most common type of heart diseases, is currently associated with high mortality in the United States. To systemically identify novel protein biomarkers associated with CAD progression for early diagnosis...

journal_title：Proteomics

authors： Jing L,Parker CE,Seo D,Hines MW,Dicheva N,Yu Y,Schwinn D,Ginsburg GS,Chen X

更新日期：2011-07-01 00:00:00

abstract：:Voltage-gated Kv7.2 potassium channels regulate neuronal excitability. The gating of these channels is tightly controlled by various mediators and neurotransmitters acting via G protein-coupled receptors; the underlying signaling cascades involve phosphatidylinositol-4,5-bisphosphate (PIP2 ), Ca2+ /calmodulin, and pho...

journal_title：Proteomics

authors： Erdem FA,Salzer I,Heo S,Chen WQ,Jung G,Lubec G,Boehm S,Yang JW

更新日期：2017-10-01 00:00:00

abstract：:High-density lipoprotein (HDL) is the most abundant lipoprotein particle in the plasma and a negative risk factor of atherosclerosis. By using a proteomic approach it is possible to obtain detailed information about its protein content and protein modifications that may give new information about the physiological rol...

journal_title：Proteomics

authors： Karlsson H,Leanderson P,Tagesson C,Lindahl M

更新日期：2005-04-01 00:00:00

abstract：:Most proteins in all organisms undergo crucial N-terminal modifications involving N-terminal methionine excision, N-alpha-acetylation or N-myristoylation (N-Myr), or S-palmitoylation. We investigated the occurrence of these poorly annotated but essential modifications in proteomes, focusing on eukaryotes. Experimental...

journal_title：Proteomics

authors： Martinez A,Traverso JA,Valot B,Ferro M,Espagne C,Ephritikhine G,Zivy M,Giglione C,Meinnel T

更新日期：2008-07-01 00:00:00

abstract：:Following transmission to the vertebrate host, the protozoan parasite Leishmania donovani differentiates into the pathogenic amastigote stage that is adapted for intracellular survival. This developmental transition is induced by environmental factors including elevated temperature and acidic pH and is likely transduc...

journal_title：Proteomics

authors： Morales MA,Watanabe R,Laurent C,Lenormand P,Rousselle JC,Namane A,Späth GF

更新日期：2008-01-01 00:00:00

abstract：:Rhodococcus sp. strain TFB is a versatile gram-positive bacterium able to grow on a wide variety of aromatic compounds as carbon and energy sources. Since the strain is refractory to genetic analysis, a proteomic approach was used to study the metabolic pathways involved in the catabolism of such compounds by analyzin...

journal_title：Proteomics

authors： Tomás-Gallardo L,Canosa I,Santero E,Camafeita E,Calvo E,López JA,Floriano B

更新日期：2006-04-01 00:00:00

abstract：:Serine/threonine protein phosphatase 1 (PP1) regulates multiple cellular processes. Protein phosphorylation-dephosphorylation is largely altered during ischemia and subsequent reperfusion. The brain is particularly vulnerable to stress resulting from ischemia-reperfusion (IR), however, the acquisition of ischemic tole...

journal_title：Proteomics

authors： Cid C,Garcia-Bonilla L,Camafeita E,Burda J,Salinas M,Alcazar A

更新日期：2007-09-01 00:00:00

abstract：:Two-dimensional electrophoresis is a widely used method for separating a large number of proteins from complex protein mixtures and for revealing differential patterns of protein expressions. In the computer-assisted proteome research, the comparison of protein separation profiles involves several heuristic steps, ran...

journal_title：Proteomics

authors： Salmi J,Aittokallio T,Westerholm J,Griese M,Rosengren A,Nyman TA,Lahesmaa R,Nevalainen O

更新日期：2002-11-01 00:00:00

abstract：:Due to the limited applicability of conventional protein identification methods to the proteomes of organisms with unsequenced genomes, researchers have developed approaches to identify proteins using mass spectrometry and sequence similarity database searches. Both the integration of mass spectrometry with bioinforma...

journal_title：Proteomics

authors： Liska AJ,Shevchenko A

更新日期：2003-01-01 00:00:00

abstract：:The 26S proteasome is a multicatalytic protease complex that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core (the 20S proteasome) as well as regulatory particles, which contain six ATPase (Rpt) subunits involved in unfolding and translocation of substrates to the catalytic chambe...

journal_title：Proteomics

authors： Kimura Y,Kurata Y,Ishikawa A,Okayama A,Kamita M,Hirano H

更新日期：2013-11-01 00:00:00

abstract：:As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restr...

journal_title：Proteomics

authors： Ge P,Hao P,Cao M,Guo G,Lv D,Subburaj S,Li X,Yan X,Xiao J,Ma W,Yan Y

更新日期：2013-10-01 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are implicated in a range of human diseases, some of which are associated with the ability to bind to lipids. Although the presence of solvent-exposed hydrophobic regions in IDPs should favor their interactions with low-molecular-weight hydrophobic/amphiphilic compounds, this h...

journal_title：Proteomics

authors： Deryusheva E,Nemashkalova E,Galloux M,Richard CA,Eléouët JF,Kovacs D,Van Belle K,Tompa P,Uversky V,Permyakov S

更新日期：2019-03-01 00:00:00

abstract：:Modification through beta-elimination has proven to be a reliable first step in the approach for enrichment of serine/threonine-phopshorylated (Ser-/Thr) peptides. However, under harsh basic conditions, Ser-/Thr-glycosylated peptides are susceptible to beta-elimination as well. Therefore, we have optimized these condi...

journal_title：Proteomics

authors： Poot AJ,Ruijter E,Nuijens T,Dirksen EH,Heck AJ,Slijper M,Rijkers DT,Liskamp RM

更新日期：2006-12-01 00:00:00

abstract：:Mitochondria were isolated from whole hearts of Dahl salt sensitive (SS) and chromosome 13 consomic control (SS.13BN/Mcwi) rats using a mechanical homogenization process followed by density centrifugation. The proteins present in the two mitochondria preparations were quantified; equal amounts of protein from each sam...

journal_title：Proteomics

authors： Smith JR,Matus IR,Beard DA,Greene AS

更新日期：2008-02-01 00:00:00

abstract：:Plasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two-thirds of the known protein targets for existing drugs. Consequently, defining membrane proteomes ...

journal_title：Proteomics

authors： Josic D,Clifton JG

更新日期：2007-08-01 00:00:00

abstract：:White spot syndrome virus (WSSV) is a pathogen that causes considerable mortality of the farmed shrimp, Penaeus monodon. Candidate 'vaccines', WSSV envelope protein VP28 and formalin-inactivated WSSV, can provide short-lived protection against the virus. In this study, P. monodon was orally intubated with the aforemen...

journal_title：Proteomics

authors： Kulkarni AD,Kiron V,Rombout JH,Brinchmann MF,Fernandes JM,Sudheer NS,Singh BI

更新日期：2014-07-01 00:00:00

abstract：:The ubiquitin-proteasome system allows the targeted degradation of proteins and plays a critical role in the regulation of many cellular processes. Proteasome inhibition is a recent antitumor therapeutic strategy and bortezomib was the first proteasome inhibitor approved for clinical use. In this study, we used the NB...

journal_title：Proteomics

authors： Uttenweiler-Joseph S,Bouyssié D,Calligaris D,Lutz PG,Monsarrat B,Burlet-Schiltz O

更新日期：2013-01-01 00:00:00

abstract：:Simpson-Golabi-Behmel syndrome (SGBS) is an X-linked condition shown to be the result of deletions of the glypican-3 (GPC3) gene. GPC3 is a proteoglycan localized to the cell membrane via a glycosylphosphatidyl-inositol (GPI) anchor. To further elucidate the GPC3 function(s), we have screened various cell lines for pr...

journal_title：Proteomics

authors： Davoodi J,Kelly J,Gendron NH,MacKenzie AE

更新日期：2007-06-01 00:00:00

abstract：:The phosphorylation of heat shock protein 27 (HSP27) occurs differently in human renal cell carcinoma (RCC) compared to homologous normal kidney tissue. Two-dimensional electrophoresis was used to separate and visualize HSP27, via immunostaining with anti-HSP27 antibody, in tumor and normal renal samples, obtained aft...

journal_title：Proteomics

authors： Tremolada L,Magni F,Valsecchi C,Sarto C,Mocarelli P,Perego R,Cordani N,Favini P,Galli Kienle M,Sanchez JC,Hochstrasser DF,Corthals GL

更新日期：2005-02-01 00:00:00

abstract：:Most proteins execute their functions through interacting with other proteins. Thus, understanding protein-protein interactions (PPIs) is essential to decipher biological functions in a living cell. To predict large-scale PPIs, effective and efficient computational approaches are desirable to integrate heterogeneous d...

journal_title：Proteomics

authors： Lin X,Chen XW

更新日期：2013-01-01 00:00:00

abstract：:Brain development and aging is a complex process involving proliferation, differentiation and apoptosis. Elucidating proteome changes in these processes can help to understand the mechanisms of brain development and maintenance as well as neurodegenerative diseases. The research reported here is a contribution to the ...

journal_title：Proteomics

authors： Föcking M,Boersema PJ,O'Donoghue N,Lubec G,Pennington SR,Cotter DR,Dunn MJ

更新日期：2006-09-01 00:00:00

abstract：:To investigate the dynamic cellular response to a condition change, selective labeling of the nascent proteome is necessary. Here, we report a method combining click chemistry protein labeling with 2D DIGE. To test the relevance of the method, we compared nascent proteomes of actively growing bacterial cells with that...

journal_title：Proteomics

authors： Osterman IA,Ustinov AV,Evdokimov DV,Korshun VA,Sergiev PV,Serebryakova MV,Demina IA,Galyamina MA,Govorun VM,Dontsova OA

更新日期：2013-01-01 00:00:00

abstract：:Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...

journal_title：Proteomics

authors： ten Have S,Boulon S,Ahmad Y,Lamond AI

更新日期：2011-03-01 00:00:00

abstract：:GlycoMod (http://www.expasy.ch/tools/glycomod/) is a software tool designed to find all possible compositions of a glycan structure from its experimentally determined mass. The program can be used to predict the composition of any glycoprotein-derived oligosaccharide comprised of either underivatised, methylated or ac...

journal_title：Proteomics

authors： Cooper CA,Gasteiger E,Packer NH

更新日期：2001-02-01 00:00:00

abstract：:Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60 min at 8...

journal_title：Proteomics

authors： Egan B,Dowling P,O'Connor PL,Henry M,Meleady P,Zierath JR,O'Gorman DJ

更新日期：2011-04-01 00:00:00

abstract：:Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...

journal_title：Proteomics

authors： Corrigan L,Jefferies C,Clive Lee T,Daly J

更新日期：2011-08-01 00:00:00

abstract：:The ability to obtain the accurate mass of a protein in a complex sample mixture aids in determining its correct in vivo form. This is important when identifying post-translationally modified proteins, protein variants or isoforms. The central technique used to separate proteins, 2-dimensional gel electrophoresis offe...

journal_title：Proteomics

authors： Fung KY,Askovic S,Basile F,Duncan MW

更新日期：2004-10-01 00:00:00

abstract：:Carcinoma of the prostate (CaP) is the second leading cause of cancer-related mortality among American men. While high cure rates are associated with localized CaP, no cure exists for advanced recurrent disease. At present there are no known serologic biomarkers specific to this stage of the disease. Several groups ha...

journal_title：Proteomics

authors： Lam YW,Mobley JA,Evans JE,Carmody JF,Ho SM

更新日期：2005-07-01 00:00:00

abstract：:Acceleration of liver regeneration could be of great clinical benefit in various liver-associated diseases. However, at present little is known about therapeutic interventions to enhance this regenerative process. Our limited understanding and the complexity of the mechanisms involved have prevented the identification...

journal_title：Proteomics

authors： Strey CW,Winters MS,Markiewski MM,Lambris JD

更新日期：2005-01-01 00:00:00

abstract：:The proteins recovered in exhaled breath condensate (EBC) might be used to non-invasively monitor respiratory diseases. However, the range of proteins and their source are still unresolved and contamination by saliva or a similar protein pattern in the nasal and bronchial compartments may make interpretation of the da...

journal_title：Proteomics

authors： Griese M,Noss J,von Bredow C

更新日期：2002-06-01 00:00:00