Discovery of biomarker candidates for coronary artery disease from an APOE-knock out mouse model using iTRAQ-based multiplex quantitative proteomics.

abstract：:In an attempt to improve the detection of peroxisome proliferation as a biomarker in environmental pollution assessment, we have applied a novel approach based on peroxisomal proteomics. Peroxisomal proteins from digestive glands of mussels Mytilus galloprovincialis were analyzed using 2-DE and MS. We have generated a...

journal_title：Proteomics

authors： Mi J,Orbea A,Syme N,Ahmed M,Cajaraville MP,Cristóbal S

更新日期：2005-10-01 00:00:00

abstract：:CD28 delivers a co-stimulatory signal for T cell antigen receptor induced activation of T cells through a mechanism which remains mostly elusive to date. In order to try and gain insight into CD28 function, we therefore applied state-of-the-art mass spectrometric protein identification technology to the analysis of CD...

journal_title：Proteomics

authors： Heller M,Watts JD,Aebersold R

更新日期：2001-01-01 00:00:00

abstract：:The skin provides protection against environmental stress. However, intrinsic and extrinsic aging causes significant alteration to skin structure and components, which subsequently impairs molecular characteristics and biochemical processes. Here, we have conducted an immunohistological investigation and established t...

journal_title：Proteomics

authors： Fang JY,Wang PW,Huang CH,Chen MH,Wu YR,Pan TL

更新日期：2016-10-01 00:00:00

abstract：:Sporozoites of the malaria parasite Plasmodium are transmitted by mosquitoes and infect the liver for an initial and obligatory round of replication, before exponential multiplication in the blood and onset of the disease. Sporozoites and liver stages provide attractive targets for malaria vaccines and prophylactic dr...

journal_title：Proteomics

authors： Hamada S,Pionneau C,Parizot C,Silvie O,Chardonnet S,Marinach C

更新日期：2021-01-16 00:00:00

abstract：:The complete sequencing of the Mycobacterium tuberculosis genome offers a unique opportunity to fully elucidate the biology of this human pathogen. One aspect of significant importance is the definition of T cell antigens. This report describes the development and implementation of a proteomic approach to defining suc...

journal_title：Proteomics

authors： Covert BA,Spencer JS,Orme IM,Belisle JT

更新日期：2001-04-01 00:00:00

abstract：:Pollution of soils by heavy metals is an ever-growing problem throughout the world, and is the result of human activities as well as geochemical weathering of rocks and other environmental causes such as volcanic eruptions, acid rain and continental dusts. Plants everywhere are continuously exposed to metal-contaminat...

journal_title：Proteomics

authors： Ahsan N,Renaut J,Komatsu S

更新日期：2009-05-01 00:00:00

abstract：:The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. Here we present the characterisation of the complexes formed intracellularly by the influenza polymerase in human cells. The virus polymerase was expressed ...

journal_title：Proteomics

authors： Jorba N,Juarez S,Torreira E,Gastaminza P,Zamarreño N,Albar JP,Ortín J

更新日期：2008-05-01 00:00:00

abstract：:It becomes increasingly clear that separation of pure cell populations provides a uniquely sensitive and accurate approach to protein profiling in biological systems and opens up a new area for proteomic analysis. The method we described could simultaneously isolate population of hepatocytes (HCs), hepatic stellate ce...

journal_title：Proteomics

authors： Liu W,Hou Y,Chen H,Wei H,Lin W,Li J,Zhang M,He F,Jiang Y

更新日期：2011-09-01 00:00:00

abstract：:The solubilization of a large array of proteins before electrophoresis itself is a very critical point for proteomic analyses. We compared the efficiency of several different solubilization buffers. From this work, we defined a very efficient solubilization buffer, including two chaotropes, two reducing agents (R2), t...

journal_title：Proteomics

authors： Méchin V,Consoli L,Le Guilloux M,Damerval C

更新日期：2003-07-01 00:00:00

abstract：:With recent publications of several large-scale protein-protein interaction (PPI) studies, the realization of the full yeast interaction network is getting closer. Here, we have analysed several yeast protein interaction datasets to understand their strengths and weaknesses. In particular, we investigate the effect of...

journal_title：Proteomics

authors： Björklund AK,Light S,Hedin L,Elofsson A

更新日期：2008-11-01 00:00:00

abstract：:Human colostrum is an important source of protective, nutritional and developmental factors for the newborn. We have investigated the low abundance proteins in the aqueous phase of human colostrum, after depletion of the major proteins secretory IgA, lactoferrin, alpha-lactalbumin and HSA by immunoabsorption, using 2-...

journal_title：Proteomics

authors： Palmer DJ,Kelly VC,Smit AM,Kuy S,Knight CG,Cooper GJ

更新日期：2006-04-01 00:00:00

abstract：:Identification of mucin-type O-glycosylated proteins with known functions in model organisms like Drosophila could provide keys to elucidate functions of the O-glycan moiety and proteomic analyses of O-glycoproteins in higher eukaryotes remain a challenge due to structural heterogeneity and a lack of efficient tools f...

journal_title：Proteomics

authors： Schwientek T,Mandel U,Roth U,Müller S,Hanisch FG

更新日期：2007-09-01 00:00:00

abstract：:Non-DNA microarrays, such as protein, peptide and small molecule microarrays, can potentially revolutionize the high-throughput screening tools currently used in basic and pharmaceutical research. However, fundamental obstacles remain that limit their rapid and widespread implementation as an alternative bioanalytical...

journal_title：Proteomics

authors： Ofir K,Berdichevsky Y,Benhar I,Azriel-Rosenfeld R,Lamed R,Barak Y,Bayer EA,Morag E

更新日期：2005-05-01 00:00:00

abstract：:Isolated from Elephantopus scaber L., a Chinese medicinal herb that is widely used to prevent and treat cancers in China, isodeoxyelephantopin (ESI) exerted antitumor effects on several cancer cells. However, its antitumor mechanism is still not clear. In this study, we found that ESI could induce G2/M arrest and subs...

journal_title：Proteomics

authors： Yan GR,Tan Z,Wang Y,Xu ML,Yu G,Li Y,He QY

更新日期：2013-11-01 00:00:00

abstract：:Puccinia triticina causes leaf rust, a disease that causes annual yield losses in wheat. It is an obligate parasite that invades the host leaf and forms intracellular structures called haustoria, which obtain nutrients and suppress host immunity using secreted proteins called effectors. Since effector proteins act at ...

journal_title：Proteomics

authors： Rampitsch C,Günel A,Beimcik E,Mauthe W

更新日期：2015-04-01 00:00:00

abstract：:High hydrostatic pressure (HHP) exerts diverse effects on microorganisms, leading to stress response and cell death. While inactivation of microorganisms by lethal HHP is well investigated in the context of food preservation and the hygienic safety of minimal food processes, sublethal HHP stress response and its effec...

journal_title：Proteomics

authors： Hörmann S,Scheyhing C,Behr J,Pavlovic M,Ehrmann M,Vogel RF

更新日期：2006-03-01 00:00:00

abstract：:Comparative proteome profiling using stable isotope peptide labelling and mass spectrometry has emerged as a promising strategy. Here, we show the broad potential of our proprietary protein sequence tag (PST) technology. A special feature of PST is its ability to detect a wide variety of proteins including the pharmac...

journal_title：Proteomics

authors： Kuhn K,Prinz T,Schäfer J,Baumann C,Schärfke M,Kienle S,Schwarz J,Steiner S,Hamon C

更新日期：2005-06-01 00:00:00

abstract：:Glycosylation is a widespread PTM of proteins; the carbohydrate moieties provide various functional, immunological and structural aspects of both eukaryotic and prokaryotic glycoproteins. Traditional strategies used to analyse glycoprotein O-glycans involve glycoprotein isolation, followed by glycan release using solu...

journal_title：Proteomics

authors： Taylor AM,Holst O,Thomas-Oates J

更新日期：2006-05-01 00:00:00

abstract：:In an accompanying study (in this issue, DOI 10.1002/pmic.200402044), we have characterised the proteome of Sca-1(+) progenitor cells, which may function as precursors of vascular smooth muscle cells (SMCs). In the present study, we have analysed and mapped protein expression in aortic SMCs of mice, using 2-DE, MALDI-...

journal_title：Proteomics

authors： Mayr U,Mayr M,Yin X,Begum S,Tarelli E,Wait R,Xu Q

更新日期：2005-11-01 00:00:00

abstract：:Two-dimensional electrophoresis is a widely used method for separating a large number of proteins from complex protein mixtures and for revealing differential patterns of protein expressions. In the computer-assisted proteome research, the comparison of protein separation profiles involves several heuristic steps, ran...

journal_title：Proteomics

authors： Salmi J,Aittokallio T,Westerholm J,Griese M,Rosengren A,Nyman TA,Lahesmaa R,Nevalainen O

更新日期：2002-11-01 00:00:00

abstract：:Affinity purification coupled to mass spectrometry (AP-MS) is gaining widespread use for the identification of protein-protein interactions. It is unclear, however, whether typical AP sample complexity is limiting for the identification of all protein components using standard one-dimensional LC-MS/MS. Multidimensiona...

journal_title：Proteomics

authors： Dunham WH,Larsen B,Tate S,Badillo BG,Goudreault M,Tehami Y,Kislinger T,Gingras AC

更新日期：2011-07-01 00:00:00

abstract：:Surface-enhanced laser desorption/ionization (SELDI) time of flight (TOF) is a mass spectrometry technology for measuring the composition of a sampled protein mixture. A mass spectrum contains peaks corresponding to proteins in the sample. The peak areas are proportional to the measured concentrations of the correspon...

journal_title：Proteomics

authors： Dijkstra M,Roelofsen H,Vonk RJ,Jansen RC

更新日期：2006-10-01 00:00:00

abstract：:Cofilin, an actin-binding protein, regulates the rate, nature and extent of assembly of the actin cytoskeleton. Native Phast gels show that the addition of cofilin to an actin-DNase I complex (74 kDa) results in the formation of a ternary complex of 94 kDa indicating an equimolar stoichiometry in the ternary complex. ...

journal_title：Proteomics

authors： Nosworthy NJ,Kekic M,dos Remedios CG

更新日期：2001-12-01 00:00:00

abstract：:We report on quality control performed in the context of a large-scale, multi-institutional study of the immune response in blood samples from prostate cancer patients. The measurements were performed by two commercially available techniques/services: protein arrays and an automated bead-based ELISA-like technique on ...

journal_title：Proteomics

authors： Ghevaria N,Visser M,Hoffmann R

更新日期：2012-09-01 00:00:00

abstract：:Aspergillus fumigatus faces abrupt changes in oxygen concentrations at the site of infection. An increasing number of studies has demonstrated that elevated production of intracellular reactive oxygen species (ROS) under low oxygen conditions plays a regulatory role in modulating cellular responses for adaptation to h...

journal_title：Proteomics

authors： Shekhova E,Ivanova L,Krüger T,Stroe MC,Macheleidt J,Kniemeyer O,Brakhage AA

更新日期：2019-03-01 00:00:00

abstract：:As one of the most important post-translational modifications, the discovery, isolation, and identification of glycoproteins are becoming increasingly important. In this study, a Con A-magnetic particle conjugate-based method was utilized to selectively isolate the glycoproteins and their glycomes from the healthy don...

journal_title：Proteomics

authors： Yang G,Cui T,Wang Y,Sun S,Ma T,Wang T,Chen Q,Li Z

更新日期：2013-05-01 00:00:00

abstract：:Long-lived conifer trees depend on both constitutive and induced defenses for resistance against a myriad of potential pathogens and herbivores. In species of spruce (Picea spp.), several of the late events of pathogen-, insect-, or elicitor-induced defense responses have previously been characterized at the anatomica...

journal_title：Proteomics

authors： Lippert DN,Ralph SG,Phillips M,White R,Smith D,Hardie D,Gershenzon J,Ritland K,Borchers CH,Bohlmann J

更新日期：2009-01-01 00:00:00

abstract：:A vast number of human cell lines are available for cell culture model-based studies, and as such the potential exists for discrepancies in findings due to cell line selection. To investigate this concept, the authors determine the relative protein abundance profiles of a panel of eight diverse, but commonly studied h...

journal_title：Proteomics

authors： Liu X,Gygi SP,Paulo JA

更新日期：2021-01-01 00:00:00

abstract：:ESAT-6 (the 6 kDa early secreted antigenic target) protein species in short-term culture filtrate of Mycobacterium tuberculosis were separated in a 4-5 narrow range pI gradient two-dimensional gel electrophoresis (2-DE). Eight ESAT-6 protein species were analyzed in detail by peptide mass fingerprinting matrix-assiste...

journal_title：Proteomics

authors： Okkels LM,Müller EC,Schmid M,Rosenkrands I,Kaufmann SH,Andersen P,Jungblut PR

更新日期：2004-10-01 00:00:00

abstract：:The proteins recovered in exhaled breath condensate (EBC) might be used to non-invasively monitor respiratory diseases. However, the range of proteins and their source are still unresolved and contamination by saliva or a similar protein pattern in the nasal and bronchial compartments may make interpretation of the da...

journal_title：Proteomics

authors： Griese M,Noss J,von Bredow C

更新日期：2002-06-01 00:00:00