Abstract:
:A large repertoire of immunological methods permits monitoring the interaction of antibodies with their specific antigen. However, recognition of a protein by a conformation-specific antibody represents a challenge because native conditions must be kept throughout the assay. Native immunoblotting of blue native gels conserves the native state by using Tween 20 instead of methanol for the obligatory destaining of the blot membrane. We validate the new technique with a set of monoclonal antibodies against respiratory NADH:ubiquinone oxidoreductase.
journal_name
Proteomicsjournal_title
Proteomicsauthors
Zickermann V,Wumaier Z,Wrzesniewska B,Hunte C,Schägger Hdoi
10.1002/pmic.200900468subject
Has Abstractpub_date
2010-01-01 00:00:00pages
159-63issue
1eissn
1615-9853issn
1615-9861journal_volume
10pub_type
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